The results of a field experiment to study the influence of biological preparations on the dynamics of mobile forms of nitrogen in 2021-2023 are presented. The tests were carried out in the conditions of the Rostov region, on ordinary carbonate chernozems. The experimental design included bacterial (“Planriz”, “Geostim”) and humic (“BIO-Don 10”, potassium humate) preparations, as well as different nutrition levels (without fertilizers and azofoska 250 kg/ha). Mineral nitrogen is characterized by high mobility; data on hydrothermal conditions and the dynamics of ammonium and nitrate nitrogen by year and nutrition level are presented. It has been established that the use of humic preparations stimulates plant nutrition, which is expressed in negative dynamics compared to the control; this effect is enhanced by the combined use of bacterial and humic preparations. As a result, an increase in yield up to 28.0 % compared to the control and up to 24.9 % compared to the background.