This paper analyzes the spatiotemporal patterns, water yield and water conservation function of different land use types in Poyang Lake Region, China, during 1990–2020 by using national land use, meteorological, soil, DEM data, etc., based on the InVEST model. The results showed that: (1) Cultivated land, forestland and water area were the main land use types in Poyang Lake Region during 1990–2020. Construction land and forestland were increasing, while grassland, unused land, cultivated land and water area were decreasing. (2) The increasing construction land was mainly derived from cultivated land. Mutual transfer existed between cultivated land and forestland, as well as between cultivated land and water area. (3) With a downward–upward–downward fluctuating trend, the average annual water yield of Poyang Lake Region was 16.17 × 109 m³, and the water conservation was 53.11 × 108 m³. The average water conservation capacity was 270.98 mm. The vegetation cover area with high water conservation value was mainly concentrated in the northwest of Jiujiang City and the northeast of Poyang County. (4) The average water conservation of different land use types during 1990–2020 was ranked as follows: water area > cultivated land > forestland > construction land > grassland > unused land. The water conservation capacity was ranked as follows: water area > grassland > forestland > cultivated land > construction land > unused land.
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