The orthogonal frequency and code division multiplexing (OFCDM) system with 2-D spreading (time- and frequency-domain spreading) is becoming a promising candidate for future broadband wireless communication systems. OFCDM is more attractive than orthogonal frequency-division multiplexing (OFDM) both by introducing frequency-domain spreading for frequency diversity provision and time-domain spreading for flexible data rate provision. To provide high-speed mobile services, multicode transmission is employed in conjunction with OFCDM. In a Gaussian or flat-fading channel, multicode channels are orthogonal. However, in a realistic wireless channel, the orthogonality no longer maintains. Thus, multicode interference (MCI) is caused. This paper focuses on the investigation of the effect of Doppler shift on the downlink transmission of high-speed mobile OFCDM systems. A practical channel estimation algorithm based on a code-multiplexed pilot channel is employed to track the variations of fading channels. Hybrid MCI cancellation and minimum mean-square error (MMSE) detection proposed by the authors is employed as an efficient way to eliminate the MCI in the frequency domain. The system performance is analytically studied with imperfect channel estimation to show how it is affected by parameters such as the window size in the channel estimation, Doppler shift, the number of stages of the hybrid detection, the power ratio of pilot to data channels, spreading factor, and so on