Caloric Density Research Articles

Erratum: Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using <em>Drosophila</em> Larvae

An erratum was issued for:Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae. A figure was updated. Figure 1was updated from: Figure 1: The sucrose-yeast (SY) diets used in our protocol. (A) The blue dots represent the isocaloric (248 calories/L) macronutrient balancing diets used in the feeding assay, which differ in the protein to carbohydrate (P:C) ratios: 1:1, 1:4 and 1:16. The beige dot represents the diet used to rear the experimental third-instar larvae (L3), which contained a P:C ratio of 1:2 and a caloric density of 495 calories/L. (B) Detailed composition and nutritional information of the sucrose-yeast (SY) based diets. The components are the same for all the diets: agar, sucrose and yeast. The amount in grams of the components needed to prepare 1 L of diet is shown. Note that 1% (v/v) of blue dye must be added to the macronutrient balancing diets and to the L3 rearing diet nipagin and propionic acid solutions must be added to a final concentration (v/v) of 3% and 0.3%, respectively. Please click here to view a larger version of this figure. to: Figure 1: The sucrose-yeast (SY) diets used in our protocol. (A) The blue dots represent the isocaloric (248 calories/L) macronutrient balancing diets used in the feeding assay, which differ in the protein to carbohydrate (P:C) ratios: 1:1, 1:4 and 1:16. The beige dot represents the diet used to rear the experimental third-instar larvae (L3), which contained a P:C ratio of 1:2 and a caloric density of 495 calories/L. (B) Detailed composition and nutritional information of the sucrose-yeast (SY) based diets. The components are the same for all the diets: agar, sucrose and yeast. The amount in grams of the components needed to prepare 1 L of diet is shown. Note that 1% (v/v) of blue dye must be added to the macronutrient balancing diets and to the L3 rearing diet nipagin and propionic acid solutions must be added to a final concentration (v/v) of 3% and 0.3%, respectively. Please click here to view a larger version of this figure.

Supplementation Practices and Donor Milk Use in US Well-Newborn Nurseries.

Guidelines encourage exclusive breastfeeding for healthy newborns but lack specificity regarding criteria for medically indicated supplementation, including type, timing, and best practices. We set out to describe practice patterns and provider perspectives regarding medically indicated supplementation of breastfeeding newborns across the United States. From 2017 to 2018, we surveyed the Better Outcomes through Research for Newborns representative from each Better Outcomes through Research for Newborns hospital regarding practices related to medically indicated supplementation. We used descriptive statistics to compare practices between subgroups defined by breastfeeding prevalence and used qualitative methods and an inductive approach to describe provider opinions. Of 96 providers representing discrete hospitals eligible for the study, 71 participated (74% response rate). Practices related to criteria for supplementation and pumping and to type and caloric density of supplements varied widely between hospitals, especially for late preterm infants, whereas practices related to lactation consultant availability and hand expression education were more consistent. The most commonly reported criterion for initiating supplementation was weight loss of ≥10% from birth weight, and bottle-feeding was the most commonly reported method; however, practices varied widely. Donor milk use was reported at 20 (44%) hospitals with ≥81% breastfeeding initiation and 1 (4%) hospital with <80% breastfeeding initiation (P = .001). Strategies related to supplementation vary among US hospitals. Donor milk availability is concentrated in hospitals with the highest prevalence of breastfeeding. Implementation of evidence-based management of supplementation among US hospitals has the potential to improve the care of term and late preterm newborns.

Effect of Feeding Strategies on the Development of Fulminant Necrotizing Enterocolitis

Necrotizing enterocolitis (NEC) causes significant neonatal morbidity. A subset of infants experience precipitous decline and death from fulminant-NEC (F-NEC). We sought to determine the effect of feeding practices on the development of this more virulent form of NEC. Premature neonates developing Bell's stage II or III NEC between May 2011 and June 2017 were reviewed. Infants were stratified as having NEC or F-NEC, defined as NEC-totalis or NEC causing rapid decline and death within 72 hours. Risk factors extracted included demographics, gestational age, and weight at NEC diagnosis. Feeding data extracted included age at first feed, caloric density, type of feed (breast milk or formula), and whether full volume feeds were reached. Univariate analysis and multivariate analysis were performed. A total of 98 patients were identified, of which 80 were included. In total, 57 patients had NEC and 23 had F-NEC. Reaching full volume feeds was associated with F-NEC on both univariate and multivariate analysis (37.9 vs. 4.5%; odds ratio: 67, 95% confidence interval: 6.606-2041, p = 0.003). Infants developing F-NEC achieved full feeds earlier (22.5 vs. 19.8 days, p = 0.025) on univariate but not multivariate analysis. There was no difference in the rates of NEC and F-NEC among infants receiving breast milk (standard or fortified) or formula (standard or increased caloric density; p = 0.235). Among premature neonates with NEC, reaching full volume feedings was associated with a nearly 70-fold increased risk of F-NEC. Assuming it was possible to predict an infant's development of NEC, alternative feeding regimens might reduce the risk of F-NEC in this population.

Cutting Calories: The Preference for Lower Caloric Density Versus Smaller Quantities Among Restrained and Unrestrained Eaters

The marketplace includes many attractive high-calorie indulgent food offerings. Despite their appeal, consumers may often be prompted to consider lower-calorie-package offerings instead (e.g., 100-calorie packs). The question thus arises: What predicts consumers’ preferences between different kinds of lower-calorie offerings? The authors conceptualize two different routes to lower-calorie versions of indulgent foods: a lower-caloric-density version (e.g., baked potato chips) or a smaller-portion-size version (e.g., a smaller bag of potato chips). The authors examine how such versions are differentially preferred and why, focusing on the key role of dietary restraint. The authors show that as dietary restraint increases, the preference for a lower-calorie version created via lower caloric density (vs. a smaller portion size) increases. Differential weights placed on health and fullness goals help explain differing preferences across dietary restraint (as the lower-caloric-density version is perceived as healthier and more filling, albeit less tasty, than the smaller-portion-size version). This framework offers theoretical implications for understanding two routes to cutting calories, practical implications for food marketers, and methodological implications for studying food choices.

Evaluation of nutrient content and caloric density in commercially available foods formulated for senior cats.

BackgroundCat foods marketed for senior cats (≥7 years) are available to owners. The variability in the nutritional content of these foods is unknown.ObjectivesTo measure the caloric density and caloric distribution of crude protein, crude fiber, crude fat, phosphorus, calcium, magnesium, sodium, potassium, and vitamin D3 in commercially available foods for senior cats and to compare nutrient content with foods for adult cats.SamplesThirty‐one senior and 59 adult commercial nontherapeutic cat food products.MethodsDescriptive study. Crude protein, crude fiber, and crude fat were measured using Dumas nitrogen combustion, Ankom filter bag technique, and acid hydrolysis, respectively. Mineral concentrations were measured using inductively coupled argon plasma‐optical emission spectroscopy. Vitamin D3 was determined by liquid chromatography with tandem mass spectrometry. Caloric density was calculated using modified Atwater values.ResultsThe evaluated nutrient concentrations in all foods for senior cats met the values of the Association of American Feed Control Officials Cat Food Nutrient Profile for adult maintenance. Foods for senior cats had significantly higher crude fiber content when compared to foods for adult cats (P < .0001). No significant difference in crude protein, crude fat and mineral concentrations was found between foods for senior and adult cats.Conclusions and Clinical ImportanceFoods marketed for senior cats are highly variable in their caloric density and nutrient content and, except for crude fiber, are similar to foods for adult cats. Veterinarians should avoid broad recommendations regarding commercially available foods for senior cats, and dietary recommendations should reflect the patient's individual needs.

Influencia del método de deshidratación sobre la calidad de snacks de zapallito redondo [Cucurbita maxima var. Zapallito (Carr.) Millán)

El creciente interés por los snacks de vegetales no amiláceos, preparados sin fritura se explica por su menordensidad calórica, respecto a la papa deshidratada y frita. Los métodos convencionales de secado a altatemperatura en ciertos casos pueden provocar pérdidas de componentes bioactivos. Es por ello que existeinterés en la búsqueda de nuevos métodos de procesamiento que reduzcan el desperdicio de estos productos. En este trabajo, se evaluó la calidad (antioxidantes fenólicos, carotenoides, azúcares, acidez, color, aceptabilidad sensorial e higroscopicidad) de snacks de zapallito redondo obtenidos por dos métodos diferentes: 1) secado con aire caliente (SAC) o 2) por un método combinado de deshidratación osmótica (DO) con NaCl seguido de una etapa de secado por aire caliente (DO+SAC). Dependiendo de la concentración de NaCl empleada, la etapa de DO permitió reducir la humedad inicial del vegetal en 15-30%. La incorporación de un pre-tratamiento de DO antes del secado por aire caliente no afectó la acidez, azúcares ni contenido de compuestos fenólicos del producto final. Los snacks obtenidos por el método combinado DO+SAC, mostraron mejor color y aceptabilidad sensorial, mayor nivel de carotenoides extraíbles y menor higroscopicidad que los producidos por SAC. Los resultados del presente trabajo muestran que los tratamientos combinados de DO y aire caliente, resultan en snacks de zapallito redondo con mejores propiedades físicas (color, higroscopicidad), nutricionales (carotenoides) y sensoriales (aceptabilidad) que aquellos obtenidos sólo por secado convencional con aire caliente.

Effects of enteral feeding on liver injury after small bowel resection

Background: The optimal regimen for enteral nutritional support in the management of children with short gut syndrome (SGS) is not well characterized. Elemental formulas do not require as much intestinal digestive and absorptive capacity. However, enteral feeding with a diet high in fat is considered beneficial due greater caloric density and greater structural adaptation. We therefore sought to determine the long-term effects of high fat diet (HFD) on liver steatosis and injury, a common complication of SGS, compared …

Factors in Early Feeding Practices That May Influence Growth and the Challenges that Arise in Growth Outcomes Research.

Growth in preterm infants is associated with improved outcomes during both the initial hospitalization and long-term. A single center investigation evaluated preterm infant in-hospital growth trajectory and how it related to early nutritional practices as a way to identify areas for quality improvement. Infants born <34 weeks’ gestational age and with discharge at or prior to 40 weeks’ gestational age were included and were divided into quartiles based on their change in weight z-score through hospitalization. Early nutritional factors were compared between the lowest and highest growth quartiles. Although the highest growth quartile experienced less days of parenteral nutrition and higher enteral caloric density in the first week, as the data was analyzed, potential statistical pitfalls became apparent. Therefore, the aim of this study was to investigate selection bias and cohort effect related to both the inclusion criteria for this cohort as well as the inherent challenges in comparison of preterm infant growth trajectories by standard fetal growth charts.

Food approach bias is moderated by the desire to eat specific foods

In a post-scarcity world, energy intake and excesses therein are mediated by psychological mechanisms, such as implicit inclinations to approach certain foods. We investigated how food deprivation, calorie content and individual food preferences affect this approach bias. Sixty women performed a touchscreen-based approach-avoidance task featuring a wide range of food items, once while satiated and once while food-deprived for 15 h. We found an overall approach bias towards food that was not influenced by food deprivation or calorie density of the food items. Instead, we found that approach bias related to the participants’ (lack of) desire to eat specific food items, and to a lesser extent to how much their general desire to eat changed due to food deprivation. Links with food preference were selective to trials in which foods had to be approached, and were absent in trials in which foods had to be avoided, pointing to selectivity to appetitive brain systems and clarifying the nature of the bias. Approach bias was unrelated to overall state or trait food craving. We conclude approach bias for appetitive stimuli may primarily express itself as speeded approach rather than slowed avoidance. Additionally, our results show there is merit in personalizing stimulus selection for approach bias measurement and retraining, as approach bias was concordant with individual food preferences, rather than objective calorie content.

Reformulation of Pastry Products to Improve Effects on Health.

Obesity is increasing at an alarming rate and has been described as a global pandemic. This increase has several explanations, including an increase in caloric intake, low levels of physical activity and the nutritional composition of our diets. In addition to public health policies based on healthy dietary patterns and recommendations based on the Mediterranean and other healthy diets, food reformulation, especially of commonly consumed processed foods, such as bakery products and pastries, is needed in the fight against obesity. Among nutritional reformulation strategies, reductions in caloric density, salt, added sugar, saturated and trans-fats are important in order to reduce the associated risk of developing chronic diseases, including cardiovascular diseases, diabetes and cancer.

Seasonal variation of total mercury and condition indices of Arctic charr (Salvelinus alpinus) in Northern Québec, Canada

The winter ecology of anadromous Arctic charr, an important fish species for Indigenous populations, has remained poorly detailed in the literature beyond descriptions of seasonal fasting and resulting declines in condition. However, prolonged periods of reduced feeding can have significant consequences for other variables, such as tissue contaminant levels. To more thoroughly detail seasonal changes, biological information (fork length, total weight, age, sex, somatic condition), stable isotopes (δ13C, % carbon, δ15N, % nitrogen), dorsal muscle % lipid, caloric densities, and total mercury (THg) concentrations were assessed in anadromous Arctic charr collected from Deception Bay, Canada, during the summer and over-wintering periods. Significant reductions in somatic condition, total weight, and % nitrogen, consistent with prolonged periods of fasting, were found for post-winter captured Arctic charr, but % lipid and caloric densities were significantly higher in these fish. THg also varied seasonally and was significantly higher in summer collected tissue. When tested individually via linear regression, significant relationships were seasonally dependent, but limited in number. All previously mentioned parameters were then incorporated into multi-variable models which better explained variations in the data. While there was no clear best model for explaining the % lipid values, caloric densities, and THg, season, condition, and stable isotope values (% carbon and % nitrogen) were the best indicators of % lipid content and caloric densities. THg concentrations were best explained by total weight, somatic condition, and δ13C. Seasonal variation in fish condition measures and THg may be indicative of condition selective mortality that yields apparent improvement through the disproportionate removal of poorer conditioned fish from the population during the over-wintering period. This hypothesis was further supported by mortality estimates and the results of the multi-predictor variable models. Collectively, this research highlights the importance of understanding seasonal dynamics for anadromous Arctic charr populations.

Effect of Maternal Health Literacy and WIC Participation on Selection of Sugar-Sweetened Beverages and Energy Dense Foods

Background: In the United States almost a quarter of children 2-5 years of age are considered overweight or obese. Many potential causes have been implicated including low maternal health literacy and high intake of sugar-sweetened beverages (SSBs). The effects of maternal health literacy, Women, Infant, and Children (WIC) participation, on selection of SSBs and energy dense foods needs to be examined. Aims: To examine correlations between maternal health literacy and selection of SSBs and energy dense foods during a feeding simulation (FS) for their children. WIC participation and SSB (or 100% juice) selection was also examined. Methods: Thirty-two mothers of 2 and 3-year-old children completed a demographic survey, and health literacy assessment (Newest Vital Sign, NVS) and a child feeding simulation (FS). For the FS, participants were presented with 37 discreet food and beverage choices with representation from each food group and some non-food group (e.g. soda, non-carbonated sugar-sweetened beverage, butter). The outcome variable comprised ratings of respondents from the NVS scale. The scale was dichotomized into two separate groups, representing “low-health-literacy” and “high-health-literacy”. Stratified analyses were conducted to examine whether percentage of calories as well as total number of calories selected daily varied by caloric density grouping of foods and beverages into “Go” (‘Almost Anytime’ foods), “Slow” (‘Sometimes’ foods), and “Whoa” (‘Once in a While’ foods). Results: Results indicate about a third of mothers had either high likelihood/possible limited health literacy. Mothers with lower scores on a health literacy assessment were not more likely to select less healthy choices. Examination of distribution of the percentage of calories consumed in three different caloric density groups were not statistically significant, indicating that there is no difference in the types of calories which are dense, or healthy by how health literate a respondent was. In addition, there was a not statistically significant difference among mean total number of daily calories consumed by health literacy level. Parents who were more likely to choose an SSB or juice during breakfast had a lower rating on the NVS questionnaire (p=0.108), whereas parents who choose a sugar-sweetened beverage or juice for lunch (p=0.305) or dinner (p=0.323) actually had a higher NVS score. However, none of these findings were statistically significant. WIC participants were significantly more likely to choose a SSB or juice for breakfast than were non-WIC participants (p=0.004). There were no significant differences in whether parents chose sugar-sweetened beverages or juice for lunch (p=0.427), dinner (p=0.433), or snack time (p=1.000). Conclusions: Health literacy and WIC participation does not appear to have an impact on selection of energy-dense foods and SSBs. Clinical implications: Health literacy is an important parameter to consider when educating patients but dietary implications may not be relevant.

Modifications to Infant Formula Instructions Improve the Accuracy of Formula Dispensing.

Readability of infant formula preparation instructions is universally poor, which may result in inaccurate infant feeding. Given that inaccurate formula dispensing can lead to altered infant growth and increased adiposity, there is an increased need for easy to follow instructions for formula preparation. We hypothesize that altering infant formula instruction labels using feedback from iterative focus groups will improve the preparation accuracy of powdered infant formula in a randomized controlled trial. Participants were recruited from the community, 18 years of age or older, willing to disclose demographic information for focus group matching, and willing to participate freely in the first (n = 21) or second (n = 150) phase of the study. In the second phase, participants were randomized to use the standard manufacturer instructions or to use the modified instructions created in the first phase. Accuracy was defined as the percent error between manufacturer-intended powder formula quantity and the amount dispensed by the participant. Participants who were assigned to the modified instructions were able to dispense the powdered formula more accurately than participants who used the standard manufacturer instructions (−0.67 ± 0.76 vs. −4.66 ± 0.74% error; p < 0.0001). Accuracy in powdered formula dispensing was influenced by bottle size (p = 0.02) but not by body mass index (p = 0.17), education level (p = 0.75), income (p = 0.7), age (p = 0.89) or caregiver status (p = 0.18). Percent error of water measurement was not different between the groups (standard: −1.4 ± 0.6 vs. modified: 0.7 ± 0.6%; p = 0.38). Thus, caloric density was more accurate in the modified instructions group compared to the standard manufacturer instructions group (−0.3 ± 0.6 vs.−2.9 ± 0.9%; p = 0.03). Infant formula label modifications using focus group feedback increased infant formula preparation accuracy.

Nutritional Value of Traditional Syrian Sweets and their Calorie Density

Information on food composition is of great importance for scientists and professionals working in the fields of nutrition and public health. The most apparent role of food composition is to provide the basis for dietary assessment and the formulation of healthier diets. Ready meals and food served in canteens are increasingly included in this approachconsidering their contribution to daily nutrition. There have been no studies that presented the nutritional values of some traditional Syrian sweets. This study was the first study to shed some lights about the nutritional facts of the traditional Syrian sweets. Seventeen differenttraditional Syrian sweets both regular calorie content (regular where sucrose was used as sweetener) and reduced calorie content (diet as Aspartame sweetener was used) were analyzed for moisture, ash, carbohydrates, protein and fat content and finally calorie density per 100 g was calculated. The study also measured the calorie content of few sweets which we did not find diet ones similar to them.The results have shown that Syrian sweets in general are very calorie dense foods due to their high content of sugar, fat and other sweeteners such as honey and High Fructose Corn Syrup (HFCS). The calorie density ranges from 347.5 Kcal to 516.2 Kcal/ 100 g serving for diet sweets and 372.8 Kcal to 532.2 Kcal/100 g serving for regular sweets. Protein ranged from 5.6 g to 18.4 g and fat from 5.5 to 29.8 g/100g serving. How to cite this article: Labban L, Thallaj N, Masri MA. Nutritional Value of Traditional Syrian Sweets and their Calorie Density. J Adv Res Food Sci Nutr 2020; 3(1): 34-41. DOI: https://doi.org/10.24321/2582.3892.202005

Dietary Protein Source and Phosphate Levels in Patients on Hemodialysis

To determine the sources of protein and phosphorus levels from the food consumed by patients on dialysis. This is a retrospective, secondary data analysis of the Comprehensive Dialysis Survey study participants who had a baseline food frequency questionnaire and baseline lab data (N=358). We examined intake of protein, phosphorus, and 7 other key nutrients from a subcohort of the Comprehensive Dialysis Survey based on the published National Kidney Foundation Kidney Dialysis Outcome Quality Initiative's nutrition recommendations. We studied the relationship of dietary protein source (plant or animal) with phosphorus intake using self-reported data from food frequency questionnaires. Variables included in final analysis are demographic, lab variables (albumin and prealbumin, alpha-1 acid glycoprotein, and C-reactive protein), and nutrition variables (calorie density, protein density, total fat, saturated fat, cholesterol, carbohydrates, phosphorus, calcium, sodium, potassium, plant-based protein, animal-based protein, and daily protein intake). Most of the patients had a lower than recommended consumption of calories, protein, phosphorus, and potassium while sodium, total, and saturated fats were overconsumed. Patients intake of dietary protein and calories was proportional to the amount of food consumed for both plant- and animal-based food. The levels of dietary protein and phosphorus were significantly (P<.05) lower in patients who primarily consumed a plant-based diet than in those who mainly consumed an animal-based diet. Consuming more plant-based protein as part of a varied diet could be nutritionally adequate while limiting intake of absorbable dietary phosphorus. More research in plant-based protein diets and their impact on patients with end-stage renal disease is needed.

A Whole Food Plant-Based Diet Is Effective for Weight Loss: The Evidence.

What does the best available balance of scientific evidence show is the optimum way to lose weight? Calorie density, water content, protein source, and other components significantly influence the effectiveness of different dietary regimes for weight loss. By "walling off your calories," preferentially deriving your macronutrients from structurally intact plant foods, some calories remain trapped within indigestible cell walls, which then blunts the glycemic impact, activates the ileal brake, and delivers prebiotics to the gut microbiome. This may help explain why the current evidence indicates that a whole food, plant-based diet achieves greater weight loss compared with other dietary interventions that do not restrict calories or mandate exercise. So, the most effective diet for weight loss appears to be the only diet shown to reverse heart disease in the majority of patients. Plant-based diets have also been found to help treat, arrest, and reverse other leading chronic diseases such as type 2 diabetes and hypertension, whereas low-carbohydrate diets have been found to impair artery function and worsen heart disease, the leading killer of men and women in the United States. A diet centered on whole plant foods appears to be a safe, simple, sustainable solution to the obesity epidemic.

Seasonal Abundance and Nutritional Concentration of Grassland Arthropods

A comprehensive understanding of foraging choices within an optimal framework requires a basic understanding of the differential nutritive concentrations and availability of prey items. Herein we present both nutritional data (e.g., the macronutrient, gross energy, and mineral concentrations) and temporal abundance of wild-caught arthropods from Nebraska prairies. Additionally, we report the size-mediated nutritional concentrations of 2 Orthoptera families: Acrididae and Tettigoniidae. Crude fat concentrations were 56%, 63%, and 53% higher in small, medium, and large Tettigoniidae than in Acrididae, respectively. Crude protein concentration varied minimally among arthropod groups (range 60.32%–76.00%), while caloric densities (gross energy) were similar among the majority of arthropod groups that were analyzed (range 16.54–23.36 kJ/g). In addition, small (early instar) orthropterans peaked synchronous to the mean first clutch hatch date for 2 species of grassland birds at the study site. Our results suggest that size and insect taxonomy influence crude fat and protein concentrations but do not appear to influence gross energy concentrations. Peak fat and protein concentration occurred during the early nestling phase for both avian species, implying synchrony between food availability and the nestling provisioning period for grassland nesting birds.

When does diet matter? The roles of larval and adult nutrition in regulating adult size traits in Drosophila melanogaster

Adult body size is determined by the quality and quantity of nutrients available to animals. In insects, nutrition affects adult size primarily during the nymphal or larval stages. However, measures of adult size like body weight are likely to also change with adult nutrition. In this study, we sought to explore the roles of nutrition throughout the life cycle on adult body weight and the size of two appendages, the wing and the femur, in the fruit fly Drosophila melanogaster. We manipulated nutrition in two ways: by varying the protein to carbohydrate content of the diet, called macronutrient restriction, and by changing the caloric density of the diet, termed caloric restriction. We employed a fully factorial design to manipulate both the larval and adult diets for both diet types. We found that manipulating the larval diet had greater impacts on all measures of adult size. Further, macronutrient restriction was more detrimental to adult size than caloric restriction. For adult body weight, a rich adult diet mitigated the negative effects of poor larval nutrition for both types of diets. In contrast, small wing and femur size caused by poor larval diet could not be increased with the adult diet. Taken together, these results suggest that appendage size is fixed by the larval diet, while those related to body composition remain sensitive to adult diet. Further, our studies provide a foundation for understanding how the nutritional environment of juveniles affects how adults respond to diet.

Influence of Megasphaera elsdenii and feeding strategies on feedlot performance, compositional growth, and carcass parameters of early weaned, beef calves.

Simmental–Angus calves [n = 135; 72 steers and 63 heifers; body weight (BW) = 212.4 kg ± 36.1] were early weaned (~5 mo) to evaluate multiple feeding regimens (conventional vs. aggressive energy diets ± Megasphaera elsdenii NCIMB 41125 (M. elsdenii culture (MEC); Lactipro Advance; MS Biotec Inc., Wamego, KS) in order to elucidate the optimal development strategy. Objectives were measured by tracking the effects of caloric density and oral drenching of growing phase performance and subsequent carcass traits. The 72-d experiment featured three groups: 1) control (CON), fed exclusively a 35% roughage diet; 2) aggressive (AGR), fed a blend of a 10% and 35% roughage diets; 3) MEC, fed the same diet as AGR and drenched with 50 mL of M. elsdenii NCIMB 41125 on day 1. A subset of calves (n = 45) was equipped with wireless rumination tags (Allflex Flex Tag; SCR Engineers, Ltd; Netanya, Israel), which logged daily rumination and general activity. Skeletal growth variables were assessed by measuring wither and hip height pretrial and posttrial. Ultrasonography provided additional resolution concerning growing phase compositional gain, which was later verified by carcass data collection. Data were analyzed as a nested analysis of variance with BW and gender serving as blocking factors. The increased caloric density of the diets administered to MEC and AGR calves resulted in greater average daily gain and gain:feed values compared with CON even during the first 21 d of diet acclimation (P ≤ 0.05). Additional fiber concentration of CON diets led to increased rumination times in 9 of the 10 wk of trial (P ≤ 0.10). No differences amongst treatments were detected for skeletal variables or ultrasound 12th rib fat. Cattle fed CON diets posted 3.4% inferior BW at the end of the growing period trial and a 3.8% reduction in hot carcass weight (HCW), reinforcing the theory that intensifying caloric intake during the growing phase does not compromise future feedlot performance. Ultrasound marbling scores for MEC-treated cattle were 19° greater than AGR treated cattle (P ≤ 0.05) at the end of the growing phase trial. Nearly the exact same advantage (22°) was observed in the cooler 5 mo later (P = 0.42). Implying MEC metabolically imprinted cattle to favor marbling development. It appears that maximizing dietary caloric density in light-weight calves does not adversely affect the growth curve, while oral dosing of MEC during the growing period may be a precursor for enhanced quality grade.

The Relation between Eating Habits and Abdominal Fat, Anthropometry, PON1 and IL-6 Levels in Patients with Multiple Sclerosis.

Background: Multiple sclerosis (MS) is a chronic neurodegenerative disease of an inflammatory, demyelinating and autoimmune nature. Diets with a high caloric density could be especially relevant in terms of the pathogenesis related to an increase in adipose tissue that is metabolically active and releases mediators, which can induce systemic inflammation and an increased oxidation state. The aim of this study was to analyse the eating habits related to calorie intake and their impact on abdominal obesity associated with anthropometric variables, the activity of the oxidation marker paraoxonase 1 (PON1), and interleukin 6 (IL-6) levelsin MS patients. Methods: An analytical and quantitative observational study was conducted with a population of 57 MS patients. The dietary-nutritional anamnesis was gained through the Food Frequency Questionnaire and a food diary. Diet and eating habits have been analysed through the Easy Diet–Programa de gestión de la consulta® software. Anthropometric measurements were taken in order to determine the presence of abdominal obesity. In addition, PON1 was quantified in serum by means of automated spectrophotometric assays and IL-6 was quantified using the ELISA technique. Results: A normal calorie intake was determined for women, yet a slightly lower intake was observed in men. Carbohydrate consumption was below what was established, and protein and lipids were over, in both cases. Furthermore, most patients had abdominal obesity, with significantly higher body mass index (BMI), waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), fat percentage and IL-6 levels. IL-6 is greatly correlated with waist circumference and WHtR. Conclusion: MS patients’ nutrient intake shows an imbalance between macronutrients. This seems to favour the abdominal obesity associated with high values of proinflammatory IL-6 that is not correlated with a lower activity of PON1.