The main intent of this study is ‘to examine the level of managers’ professional efficiency in cooperative union to enhance Agricultural cooperative unions’ business performances’. The study covers 228 respondents of sampled agricultural cooperative union. The study were employed both quantitative and qualitative research approach to answer the research question. The pertinent data related to the study was collected with the help of survey questionnaire, key informant interview and focus group discussion from the study participants. The pertinent data collected were analyzed with the help of descriptive statistics. Accordingly, the study result reveals that there were managers professional efficiency gap among managers of the sampled cooperative unions in the study. Therefore, the government, specifically the Cooperative promotion agency, concerned committees of the union must give due attention to the hiring of managers in respective of their technical and political professional efficiency and other human resource development like continuous trainings for cooperative managers on cooperative concepts, principles, values and business activities. Consequently, these actions will make sure the business competence of the cooperatives unions in the regional and national wise. Keywords: Manager, Professional Efficiency, Cooperative, Business Performance DOI: 10.7176/JAAS/77-01 Publication date: January 31 st 2022