Burn Therapy Research Articles

Effect of H. perforatum oil containing membranes on the second degree burn wounds in rats

Burn is one of the most frequently occurring cases of skin injury which needs immediate medical intervention. The aim of this study is quick and efficient therapy of second degree burns using H.perforatum oil releasing polymeric membranes. 35 adult Wistar Albino rats were used for in vivo experiments. Second degree burn wounds were created on the dorsal side of the rats using a preheated brass rod. Wound zones were photographed and wound areas was calculated on 1st, 4th, 7th, 11th and 16th days of wound formation. Situation of the wound zones were evaluated macroscopically. No adverse effects were met during treatment. At the end of experiment, skin wound zone was dissected with neighbor healthy tissues. Fixed tissues were blocked in paraffin after routine tissue inspection. Microscopic evaluations of the wound situation were done by histopathological and immunohistochemical analyses of the thin tissue specimens taken from blocked tissues. It was confirmed with macroscopic and microscopic evaluations that a more distinct wound healing and a better ratio of newly formed tissue was evident for the wound zones especially treated with oil releasing membranes.

Chemically defined and xenogeneic-free culture method for human epidermal keratinocytes on laminin-based matrices.

The basal keratinocyte progenitor cells in cultured epithelial autografts (CEAs) regenerate human epidermis after transplantation, a curative therapy for severe burns and, recently, diseases with epidermal loss, such as junctional epidermolysis bullosa (EB). Although a culturing technique for skin keratinocytes was developed four decades ago, the xenogeneic nature of that conventional CEA culture system restricts its use to the treatment of critical and life-threatening cases, such as severe burns on >30% of total body surface area and EB. In the present protocol, we describe how to implement a defined, xeno-free culture system that supports long-term ex vivo expansion of functional human epidermal keratinocytes. Skin-specific basement membrane proteins called laminins play important roles in the maintenance of phenotypic integrity and in supporting the survival of keratinocytes that are adhered to them. This fully human keratinocyte culture system is 'regulatory friendly' and increases the potential of epithelial cellular therapy, which can be expanded to treat less severe burns and other skin defects, such as chronic diabetic wounds. It takes between 7 and 14 d to obtain an initial culture. Conservatively, a secondary culture from the primary culture can be expanded up to 20-fold within 4-5 d once cells reach confluency.

Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds

Context Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healing by regulating autophagy. Objective To explore the mechanism of moist exposed MEBT/MEBO promoting diabetic wound repair. Materials and methods Eighty male Wistar rats were randomly assigned to control (n = 20) and diabetic group induced by intraperitoneal injection of STZ (n = 60), which were further randomly assigned to MEBO, Kangfuxin and model groups (n = 20 each). All rats underwent full-thickness skin resection in the back. Wound healing was dynamically observed and wound tissues were collected at five time points for pathological examination, autophagosome and the expression of PI3K, Akt and mTOR. Results The healing time in the control group was the shortest, no statistically significant difference was found between the MEBO and the Kangfuxin group (p = 0.76). The morphology of autophagosomes ranged large to small, which was the most obvious in the MEBO group. The mRNA and protein expression of PI3K, Akt and mTOR in each group reached the peak on Day 5, the levels in the MEBO group were the highest (F = 18.43, 19.97, 15.36, p < 0.05). On Day 11, the expression levels in each group began to decline. Discussion and conclusions In this study, we discussed the molecular mechanism of MEBT/MEBO promoting the repair of diabetic ulcer wounds through autophagy and PI3K-Akt-mTOR signalling pathway, which provides a new way for drug design in the future.

Ozone therapy in burns: our experience in Egypt [abstract

BACKGROUND: Burn injuries are one of the major health problems all over the world. The problem of infection in burn wounds is considered a major problem and the main cause of death in many burned patients. Ozone has been used with good results in the treatment of patients with diabetic foot, because of its germicidal properties and its influence on the processes of oxygen metabolism, and other effects.&#x0D; &#x0D; PURPOSE: We report the effect of Ozone therapy on the healing process of recent burns in the Burn unit of the Alexandria Main University Hospital and Plastic Surgery Department Faculty of Medicine, Alexandria University.&#x0D; Patients and methods: A review of two RCTs of 140 patients with recent burns done to investigate the effects of topical application of ozonized water, ozone gas (Sauna or Bagging), and ozonized olive oil in the treatment of recent burns.&#x0D; &#x0D; RESULTS: All patients manifested clinical improvement by Topical Ozone therapy. The healing time of the ozone treated wounds was significantly lower than that of the control wounds. The full-thickness infected necrotic wounds that were treated with ozone showed earlier clearance of necrotic tissue and infection than the control wounds and became ready for skin grafting in a significantly lower time than the control wounds. Histopathological studies revealed marked increase in the expression of the fibronectin and transforming growth factor-B after treatment in comparison to the control wounds.&#x0D; &#x0D; CONCLUSION: Topical application of ozone causes a highly significant reduction in the healing time of the recent burn injuries.

Protective properties of kefir on burn wounds of mice that were infected with S. aureus, P. auroginasa and E. coli

Burns and burn wounds are very sensitive to infections and cause a large amount of death worldwide. Although burn wound is sterile at the beginning, because of the risk factors such as prolonged hospital stay, immune suppression and burn affecting large surface area, colonisation with Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli occur. For the burn therapy, one of the most important ways is to control bacterial infections. A probiotic fermented milk product kefir has antioxidant, antimicrobial, antiinflammatory, anticancer and various health promoting features. This study aims to examine possible protective properties of kefir which was used on the burn wounds that were infected with S. aureus, P. auroginasa and E. coli. Swiss albino / Balb-c mice were seperated into four groups: (1) used as control group, (2) second-degree burn model+ burn wounds were infected with P.aeruginosa + S.aureus + E.coli, (3) second-burn wounds were treated with sterile pads dressed with kefir and (4) second-degree burn+burn wounds were infected with P. aeruginosa + S.aureus +E.coli before being treated with sterile pads dressed with kefir. The serum biochemical results verified the histopathological results and our findings showed that kefir is an effective product with cell-protecting properties.

Comparing the effects of silver sulfadiazine and cerium nitrate silver sulfadiazine on burn-wound healing and survival rate of rat animal model

Aim: Current studies about burn therapy are focusing on survival rate of patients with severe burned. So, here we aim to assess the effects of cerium nitrate silver sulfadiazine (CN+SSD) and compare with SSD ointment on mortality and burn healing in rat animal model. Methods: Twenty rats were used in this study. After inducing burn wounds on back skin of animals, they were classified into two groups randomly. In one group, SSD was administrated topically immediately after inducing burn wound and in the other the combination of CN+SSD was applied. The treatments in both groups were continued as administration of the ointments two times per day for 28 days. Animal’s weight and wound sizes were measured 7, 14, 21 and 28 days after burn induction in all animals. The mortality of rats as well as their wound histopathology was also evaluated. Results: On day 28, wound’s average size was decreased to 25 and 27% of initial area in SSD and CN +SSD groups, respectively. In CN+SSD group, the wound size was not smaller than SSD group significantly. Histological comparison has demonstrated no significant differences between CN+SSD group and SSD treated. Conclusion: The results of our study did not show any differences between CN+SSD and SSD topical treatments in terms of wound healing and mortality rate in rat animal model significantly.

Rehabilitation of the Burn Patient

Burn rehabilitation begins the moment the patient contacts the healthcare system and, for many patients, it never truly ends. Returning severely burned patients to their pre-injury level of function has become more challenging, as more severely burned patients survive their injury. The need for rehabilitation does not simply correspond with burn depth, total body surface area involved, or injury severity. Other factors also impact need for rehabilitation, such as anatomic location of injury (eg, across finger joints, face), additional psychosocial strain from a house fire or having an injured loved one, or body image concerns related to visible scarring. To meet these and other challenges posed by burn injuries, rehabilitation requires a multidisciplinary team including, but not limited to, nursing, surgery, burn therapy, rehabilitation medicine, vocational counseling, rehabilitation psychology, psychiatry, and nutrition. Accord­ingly, verification as a burn center by the American Burn Association requires a goal-oriented, multidisciplinary rehabilitation program. This chapter broadly discusses the spectrum and integral components of burn rehabilitation. This review contains 13 figures, 8 tables, and 71 references. Key Words: burn, community integration, function, mental health, multidisciplinary, outcome, pain, quality of life, reconstruction, rehabilitation, therapy.

Rehabilitation of the Burn Patient

Burn rehabilitation begins the moment the patient contacts the healthcare system and, for many patients, it never truly ends. Returning severely burned patients to their pre-injury level of function has become more challenging, as more severely burned patients survive their injury. The need for rehabilitation does not simply correspond with burn depth, total body surface area involved, or injury severity. Other factors also impact need for rehabilitation, such as anatomic location of injury (eg, across finger joints, face), additional psychosocial strain from a house fire or having an injured loved one, or body image concerns related to visible scarring. To meet these and other challenges posed by burn injuries, rehabilitation requires a multidisciplinary team including, but not limited to, nursing, surgery, burn therapy, rehabilitation medicine, vocational counseling, rehabilitation psychology, psychiatry, and nutrition. Accord­ingly, verification as a burn center by the American Burn Association requires a goal-oriented, multidisciplinary rehabilitation program. This chapter broadly discusses the spectrum and integral components of burn rehabilitation. This review contains 13 figures, 8 tables, and 71 references. Key Words: burn, community integration, function, mental health, multidisciplinary, outcome, pain, quality of life, reconstruction, rehabilitation, therapy.

First aid therapy for corrosive chemical eye burns: results of a 30-year longitudinal study with two different decontamination concepts

PurposeThere is currently uncertainty about the most efficacious decontamination solution for corrosive chemical eye burns. This 30-year longitudinal study evaluated the relative efficacy of two different decontamination methods. Passive decontamination consists of rinsing with tap water, 0.9% normal saline, isotonic buffered phosphate solution, or Ringer’s lactate. Active decontamination adds an amphoteric, polyvalent, and chelating component with Previn® (Diphoterine®) solution (Laboratoire Prevor, Valmondois, France).MethodsA prospective evaluation of patients treated in two specialized eye clinics for eye burns was begun in 1988. Recorded data included exposure circumstances, type of corrosive, different types of first therapy, and clinical treatment and outcome. Patients were treated from clinic admission and up to 24 h after the corrosive chemical burn with rinsing for 15 min using two different protocols. From 1988 to 2005, sterile 0.9% normal saline or Ringer’s lactate was used. Since 2006, sterile, hypertonic, amphoteric Previn® solution was used. Comparative statistical analysis was done with the Fisher contingency tables and Wilcoxon tests.ResultsThere were a total of 1495 patients with 2194 chemically burned eyes. In 1988–2005, the annual incidence was 66.1/year; in 2006–2017, it was 65.5/year. Similar incidences were noted when initial rinsing was with tap water or isotonic buffered phosphate solutions. There was a significantly more severe outcome of corrosive chemical eye burns with any first aid rinsing solutions other than Previn® solution or tap water was used (p < 0.001). Previn® solution or tap water rinsing in the pre-hospital setting and secondary rinsing with Previn® solution in the hospital decreased lesion severity in comparison with all other rinsing solutions (p < 0.001).ConclusionThe frequency of corrosive chemical eye burns was comparatively high despite tightening of occupational health and safety regulations over the past 30 years. The severity of corrosive chemical eye burns has been dramatically decreased since the introduction of Previn® solution for initial and secondary rinsing. A new protocol for immediate Previn® solution use by the Cologne Fire Brigade and secondary Previn® solution rinsing in hospital has reduced the frequency of severe corrosive chemical eye burns to less than 60% as compared to the period of 1988–2005 when other rinsing solutions were utilized.

Denatured acellular dermal matrix seeded with bone marrow mesenchymal stem cells for wound healing in mice

It is the basic task of burn therapy to cover the wound with self-healthy skin timely and effectively. However, for patients with extensive burns, autologous skin is usually insufficient, and allogenic or heterogeneous skin leads to strong immune response. It is vital to choose an appropriate treatment for deep extensive burns. Nowadays, the dermal substitute combined with bone marrow mesenchymal stem cells (BM-MSCs) is a prospective strategy for burn wound healing. Denatured acellular dermal matrix (DADM), as one of dermal substitutes, which prepared by burn skin discarded in escharotomy, not only maintains a certain degree of 3D structure of collagen, but also has good biocompatibility. In this study, the preparation method of DADM was improved and DADM was seeded with BM-MSCs. Then BM-MSCs-seeded DADM (DADM/MSCs) was implanted into mice cutaneous wound, and the effect of DADM/MSCs dermal substitute was assessed on skin regeneration. As a result, BM-MSCs survived well and DADM/MSCs scaffolds significantly promoted wound healing in terms of angiogenesis, re-epithelialization and skin appendage regeneration. DADM/MSCs scaffold may represent an alternative promising therapy for wound healing in deep extensive burns.

Multipotent adult progenitor cells improve healing of mouse burn wounds

Background & Aim Although survival rates are increasing, burn injuries remain a challenge in the field of cutaneous wound healing. Stem cells have been shown to be a potential new therapy for burns and promote wound healing through a decrease in the inflammation and increase in collagen production. Multipotent adult progenitor cells (MAPC®) are a subpopulation of bone marrow-derived stem cells with outstanding self-renewal and differentiation capacity. MAPC cells also secrete a wide range of growth factors and cytokines which have effects on cellular activities. This study aimed to examine the effects of MAPC treatment and its secretome on burn injury repair using in vitro and in vivomodels. Methods, Results & Conclusion The effect of MAPC secretome on the capacity of keratinocytes, fibroblast and endothelial cells to migrate and proliferate was determined in vitro using scratch wound closure and WST1 assay respectively. Secretome-treated fibroblasts were also immunostained for collagen 1 and 3 to investigate its effect on matrix production. Additionally, second degree burns were created on the dorsal surface of mice (n=8/group) and 5 × 105 MAPCs in 100µl PBS were administered via intradermal injection to the wound margins, 24h post-burn injury. The burns were imaged daily until day 7, for macroscopic wound area determination, when they were collected and analysed by H&E staining to determine dermal wound width and rate of re-epithelialisation. Masson's trichrome staining was also performed to investigate the total amount of collagen deposited within the burns. Cells treated with MAPC-secretome showed improved rate of scratch closure compared to controls. The proliferation of these cells was also increased when treated with MAPC-secretome. Moreover, fibroblasts treated with MAPC-secretome deposited more collagen 1 and 3. Burns intradermally injected with MAPCs showed a significant reduction in macroscopic wound area compared with controls at day 3 and day 7. Analysis of day 7 burn tissues showed significant decrease in the dermal wound width and increased rate of reepithelialisation. Masson's trichrome staining showed that collagen deposition in the wounds was significantly elevated following MAPC cell treatment. This study demonstrates the beneficial effects of MAPC cell therapy on burn injury repair including reduced time to healing and increased collagen deposition. The beneficial effect of MAPC cells may be due in part to the factors they secrete which improve skin cell proliferation and migration as well as collagen deposition.

216 Risk Stratification of Orbital Compartment Syndrome: A Comparison of Intra-Ocular Pressure and the Baux Score

Early fluid resuscitation is a mainstay in acute burn care. Aggressive fluid therapy in significant thermal burns can lead to third spacing of fluid into the orbit thus increasing the risk of orbital compartment syndrome. The Baux score is a simple tool often used in burn care to help predict mortality and thus indicate severity of the burn. This study aims to quantify the intra-ocular pressures (IOP) as it relates to the patient’s Baux score, in an effort to stratify the risk for orbital compartment syndrome. This study is a retrospective analysis of 70 patients with thermal burns at a level one burn center. The initial Baux score was noted for each patient as well as the highest measured IOP within the first 72 hour period. Linear regression was used to test for an association between peak intraocular pressure and the Baux score. Patients requiring orbital decompression were compared with those who did not, using the Fisher exact test. Eleven of 70 patients required emergent orbital decompression with a lateral canthotomy and cantholysis and had a higher Baux score than patients who did not receive the procedure (p = 0.03). The average IOP was 18.7 +/- 5mmHg but patients who underwent an orbital decompression all had IOP greater than 40. There was a positive relationship between the Baux score and IOP for Baux scores greater than 70; however, this was no longer statistically significant when adjusted for periocular burns. The risk of orbital compartment syndrome linearly increases with beaux scores greater than 70 and additionally in patients with periocular burns. As such, a high index of suspicion and early evaluation by an ophthalmologist is warranted in these patients to preserve vision. An elevated Beaux score can predict the necessity for ophthalmic intervention in patients at a high risk for vision loss secondary orbital compartment syndrome

358 Silk-Elastin Sponge is Effective for Burn Therapy after Tangential Excision

358 Silk-Elastin Sponge is Effective for Burn Therapy after Tangential Excision

Study of negative\xa0pressure wound therapy as an adjunct treatment for acute burns in children (SONATA in C): protocol for a randomised controlled trial

BackgroundAlthough negative pressure wound therapy (NPWT) is widely used in the management of several wound types, its efficacy as a primary therapy for acute burns has not yet been adequately investigated, with research in the paediatric population particularly lacking. There is limited evidence, however, that NPWT might benefit children with burns, amongst whom scar formation, wound progression and pain continue to present major management challenges. The purpose of this trial is to determine whether NPWT in conjunction with standard therapy accelerates healing, reduces wound progression and decreases pain more effectively than standard treatment alone.Methods/designA total of 104 children will be recruited for this trial. To be eligible, candidates must be under 17 years of age and present to the participating children’s hospital within 7 days of their injury with a thermal burn covering <5% of their total body surface area. Facial and trivial burns will be excluded. Following a randomised controlled parallel design, participants will be allocated to either an active control or intervention group. The former will receive standard therapy consisting of Acticoat™ and Mepitel™. The intervention arm will be treated with silver-impregnated dressings in addition to NPWT via the RENASYS TOUCH™ vacuum pump. Participants’ dressings will be changed every 3 to 5 days until their wounds are fully re-epithelialised. Time to re-epithelialisation will be studied as the primary outcome. Secondary outcomes will include pain, pruritus, wound progression, health-care-resource use (and costs), ease of management, treatment satisfaction and adverse events. Wound fluid collected during NPWT will also be analysed to generate a proteomic profile of the burn microenvironment.DiscussionThe study will be the first randomised controlled trial to explore the clinical effects of NPWT on paediatric burns, with the aim of determining whether the therapy warrants implementation as an adjunct to standard burns management.Trial registrationAustralian New Zealand Clinical Trials Registry, ACTRN12618000256279. Registered on 16 February 2018.

Efficiency of Multiparticulate Delivery Systems Loaded with Flufenamic Acid Designed for Burn Wound Healing Applications.

Burns are soft tissue injuries that require particular care for wound healing. Current tissue engineering approaches are aimed at identifying the most efficient treatment combinations to restore the tissue properties and function by using adapted scaffolds or delivery platforms for tissue repair and regeneration by triggering molecules. To reduce the inflammation associated with skin burns, the addition of an anti-inflammatory factor in these scaffolds would greatly increase the quality of the therapy. Therefore, this study is aimed at obtaining and validating a novel multiparticulate system based on a collagen matrix with controlled delivery of flufenamic acid anti-inflammatory drug for burn wound healing applications. In this work, we have characterized the properties and biocompatibility of these multiparticulate drug delivery systems (MDDS) and we have demonstrated their efficiency against burns and soft tissue lesions, particularly when the drug was microencapsulated, and thus with a controlled release. This study contributes to the advancement in therapy of burns and burn wound healing applications.

Does the transplantation of keratinocytes really reduce the risk of death? Survival analysis of patients hospitalized at the Dr Stanisław Sakiel Centre for Burns Treatment in 2008–2015

Keratinocyte transplantation is an adjuvant procedure in the extensive burn therapy method. However, it must be taken into consideration that clinical results of keratinocyte transplantation are ambiguous and progress achieved in this method is still being verified, especially due to the high cost of cultured epithelial autograft (CEA) transplants. The aim of this study was to verify the impact of cultured keratinocyte application on patients' survival. This study included a group of patients with the highest chance for a successful outcome of the therapy and excluded patients with no compelling reason to apply for such an expensive therapy. This study included all the patients with burns diagnosed between January 1, 2008 and January 1, 2016, who were treated with cultured skin cells. Patients' age and gender, percentage of total body surface area (TBSA) affected, percentage of burn depth of the 3rd/4th degree, number of days between admission and surgery, and need for rehabilitation were analyzed. The cultured cell application did not significantly affect the risk of death (p > 0.05). Keratinocytes should be applied as an adjunctive method for the treatment of burns with at least 40% TBSA affected, but with a maximal burn depth of the 2nd degree. In the group of patients below 50 years of age, a higher number of transplants with a cell population above 20 million/mL and a significantly lower mortality rate were observed, which means that in the mentioned age group, this graft was more effective. It has been suggested that patients older than 50 years of age with burns deeper than of the 2nd degree should be treated with more advanced methods like, e.g., the application of stem cells.

Effectiveness of ECMO for burn-related acute respiratory distress syndrome

IntroductionAcute respiratory distress syndrome (ARDS) is a complication that affects approximately 40% of burn patients and is associated with high mortality rates. Extracorporeal membrane oxygenation (ECMO) therapy is a management option for severe refractory hypoxemic respiratory failure; however, there is little literature reporting the effectiveness of this therapy in burns. Our study objective was to review patient outcomes in burns following severe ARDS treated with ECMO. MethodsWe retrospectively reviewed all patients treated with ECMO for ARDS who received their burn care at a single regional burn center between 9/1/2006 and 8/31/2016. Primary patient outcome examined was discharge disposition. ResultsWe identified 8 patients who had ARDS secondary to burn who were placed onto ECMO during this 10-year period. The average APACHE score, SOFA score, and P/F ratio were 21±3, 9±2, and 59±8, respectively, at the time of decision for ECMO. No ECMO-related complications were identified. Out of the 8 patients reviewed, 1 died, 4 were discharged to acute rehabilitation or a long-term acute care facility, and 3 were discharged to home. ConclusionMortality in burn patients with ARDS who are managed with ECMO is extremely low. Careful selection and timely intervention with ECMO contributed to good clinical outcomes.

Accelerate Healing of Severe Burn Wounds by Mouse Bone Marrow Mesenchymal Stem Cell-Seeded Biodegradable Hydrogel Scaffold Synthesized from Arginine-Based Poly(ester amide) and Chitosan.

Severe burns are some of the most challenging problems in clinics and still lack ideal modalities. Mesenchymal stem cells (MSCs) incorporated with biomaterial coverage of burn wounds may offer a viable solution. In this report, we seeded MSCs to a biodegradable hybrid hydrogel, namely ACgel, that was synthesized from unsaturated arginine-based poly(ester amide) (UArg-PEA) and chitosan derivative. MSC adhered to ACgels. ACgels maintained a high viability of MSCs in culture for 6 days. MSC seeded to ACgels presented well in third-degree burn wounds of mice at 8 days postburn (dpb) after the necrotic full-thickness skin of burn wounds was debrided and filled and covered by MSC-carrying ACgels. MSC-seeded ACgels promoted the closure, reepithelialization, granulation tissue formation, and vascularization of the burn wounds. ACgels alone can also promote vascularization but less effectively compared with MSC-seeded ACgels. The actions of MSC-seeded ACgels or ACgels alone involve the induction of reparative, anti-inflammatory interleukin-10, and M2-like macrophages, as well as the reduction of inflammatory cytokine TNFα and M1-like macrophages at the late inflammatory phase of burn wound healing, which provided the mechanistic insights associated with inflammation and macrophages in burn wounds. For the studied regimens of these treatments, no toxicity was identified to MSCs or mice. Our results indicate that MSC-seeded ACgels have potential use as a novel adjuvant therapy for severe burns to complement commonly used skin grafting and, thus, minimize the downsides of grafting.

Low level laser as an adjunct therapy for second degree superficial burns

Low level laser as an adjunct therapy for second degree superficial burns

Application Of Allo Mesenchymal Stem Cells On Chronic Burn Wound: Case Series Report

&#x0D; &#x0D; &#x0D; &#x0D; Background : Severe burns are among the commonly occurring trauma with lethal outcome. One of the important aspects of severe burn therapy is to quickly achieve wound healing. Previous reports indicated that mesenchymal stem cells (MSCs) therapy contributes in facilitating better wound healing. In this report, we investigated the effects of MSCs derived from human bone marrow and umbilical cord on wound healing in patients with severe burns and its mechanism.&#x0D; Method : We performed human bone marrow and human umbilical cord MSCs therapy on 3 severe burns patients. Two of the patients had inadequate donor to close raw surface with skin graft, whilst one patient had infected chronic burn wound which have failed to epithelialize despite repeated attempts of skin graft and wound care.&#x0D; Result : We observed that MSCs therapy significantly accelerated wound healing. The effects after MSCs migrated into wound were decreased infiltration of inflammatory cells and faster epithelialization.&#x0D; Conclusion : This study suggests that MSCs therapy has positive effects in improving wound healing in severe burns patients. Data provided by this research may serve as theoretical basis for further study of MSCs application in burn wound therapy.&#x0D; &#x0D; &#x0D; &#x0D;