Burned Rats Research Articles

Bufalin Regulates STAT3 Signaling Pathway to Inhibit Corneal Neovascularization and Fibrosis After Alkali Burn in Rats

Purpose Bufalin (BU) is a bioactive ingredient extracted from the skin and parotid venom glands of Bufo raddei, which can effectively inhibit angiogenesis. The aim of this study was to investigate whether BU could affect corneal neovascularization (CoNV). Methods A rat CoNV model (right eye) was constructed by administration of NaOH, and the left eye served as a control. Corneal damage scores of rats were detected. Hematoxylin & eosin, TUNEL, and Masson staining examined pathological changes, apoptosis, and fibrosis of corneal tissues. Immunohistochemistry and western blotting assessed the expression of proteins. Results BU intervention resulted in a significant reduction in corneal inflammatory cells, repair of corneal epithelial hyperplasia, significant reduction in stromal edema, and reduction in vascular proliferation. BU can inhibit corneal neovascularization. Conclusion This study demonstrated that BU inhibits CoNV, fibrosis, and inflammation by modulating the STAT3 signaling pathway, elucidating the intrinsic mechanism of its protective effect. BU has great potential in the treatment of CoNV caused by corneal alkali burns.

Enhanced Third Degree Burn Wound Healing by Hypoxic Mesenchymal Stem Cells' Secretome: IL-10 Upregulation and TNF-α/PGE2 Suppression

Background: Severe third-degree burns pose a notable obstacle in clinical settings, frequently leading to hindered healing of wounds and the development of scars. In this research, we explored how the secretome of hypoxic mesenchymal stem cells (HS-MSCs) could be improved the healing of burn wounds. Methods: In this study, we used 20 male Wistar rats that induced third degree wound by metal 80 exposure for 10 s. These rats divided into 4 groups: Healthy, control, HS-MSCs 100 µL, and HS-MSCs 200 µL groups. We injected the HS-MSCs, which carry various beneficial molecules like IL-10, TGF-β, PDGF, and VEGF, directly into the skin in a third-degree burn rat model on day 1 after burn induction, and harvest on day 14. Results: Our research discovered that using HS-MSCs resulted in a dosage-related rise in IL-10, an anti-inflammatory cytokine, alongside a notable decrease in TNF-α and PGE2, pro-inflammatory cytokines. These effects quickened the healing process by improving re-epithelialization and minimizing scar formation. Conclusions: Our findings indicate that HS-MSCs show promise as a treatment for severe burns by potentially controlling inflammation and fostering a regenerative healing process. More investigation is needed to understand the exact mechanisms and to explore how HS-MSCs could be applied in clinical treatments. HIGHLIGHTS HS-MSCs suppress inflammation and promote regenerative healing in severe burns HS-MSCs accelerated re-epithelialization and reduced scarring GRAPHICAL ABSTRACT

Stereological Investigation of the Effects of Hypericum Perforatum L. Plant on Skin Tissue in Second Degree Burns in Rats

The aim of this study was to examine stereologically the effect of Hypericum perforatum L. oil on skin injuries resulting from burns. Experimental groups were selected 18 healthy rats with an average weight of 250-300 grams as material. The rats were divided into 3 groups: control, burn and burn+treatment, with 6 in each group. In the control group, second-degree burns were created and the rats were perfused. Control group rats were kept in formaldehyde for a week. Burns were applied to the burn and burn+treatment groups with 100ºC water. 4gr/kg of Hypericum perforatum L. oil was given via gavage to the burn+treatment group only for 21 days. The burn group was not intervened. At the end of the process, rats in both the burn and burn+treatment groups were perfused. The skin of the animals, which were kept in formaldehyde for a week, and where second-degree burns occurred were dissected. After tissue tracking and tissue embedding procedures for all animals in the group, 5µm thick sections were taken to obtain 8-10 sections at a ratio of 1/75. Volume values were calculated using stereological methods from the sections photographed with an x4 objective. Calculations were made using the Shtereom I program in accordance with the Cavalieri Principle. Non-parametric tests and Kruskal Wallis test were used in statistical evaluations. When the results were analyzed, it was observed that the administration of Hypericum Perforatum L. oil by gavage had an effect on the healing process of second degree burn wounds.

The effect of acellular scaffold loaded with Wharton's jelly-derived stem cells and mineral pitch on healing of burn model in rat.

Severe burns often result in an exacerbated inflammatory response, which can contribute to further injury. This inflammatory response may lead to an increased risk of infection, multiple organ failure, and death. This study aimed to investigate the potential of reducing inflammation to enhance burn wound healing in rats using ovine's small intestinal submucosa as a carrier for Wharton's jelly mesenchymal stem cells (WJ-MSCs) and Mineral Pitch (MP). A rat burn model was developed, and the animals were divided into four groups: control group: burn, placebo group: scaffold-treated burn, cell experimental group: WJ-MSCs seeded scaffold-treated burn, and cell and MP experimental group: scaffolds loaded with WJ-MSCs and MP-treated burn. After treating the wounds in the relevant groups and sampling them on days 5, 14 and 21, histological and pathological parameters, and the expression of genes involved in angiogenesis and epithelialization were evaluated. The study results revealed several findings in the burn wounds. These included changes in mast cell populations, a decrease in inflammatory neutrophils and lymphocytes, an increase in fibroblasts and blood vessels, and upregulation of angiogenesis and epithelialization genes. These changes collectively contributed to enhanced wound healing in cell and MP experimental group compared to the other groups. The findings suggest that scaffolds loaded with Wharton's jelly-derived stem cells and MP can serve as engineered tools to modulate inflammatory conditions during the burn wound healing process. These interventions can improve burn wound management and promote better outcomes.

Formulation and evaluation of ointment containing hydroalcoholic extract derived from the bark of Moringa oleifera for wound healing activity in rat model

Background: This study aimed to assess the effectiveness of a hydroalcoholic extract derived from the bark of Moringa oleifera in facilitating the healing process of second-degree burns wounds. Moreover, a comprehensive assessment was carried out on standardized M. oleifera bark to ascertain its physiochemical characteristics, botanical compound layout, and antioxidant activity, all of which play a crucial role in its capacity to facilitate the healing process of burns. Methods: For 14 days, the efficacy of ointments containing a hydroalcoholic extract of M. oleifera bark at concentrations of 5% and 10% was evaluated for treating second-degree burns in rats. Additionally, histological analysis was conducted on skin tissue samples. Results: The M. oleifera bark extract exhibited TPC (52.56 mg/gm of dried extract) and TFC (84.33 mg/gm of dried extract) value along with antioxidant activity (IC50 value of 0.98 µg/ml) for radical scavenging, in the presence of several phytochemicals. The most favorable outcomes were achieved using a 10% ointment composition, demonstrating a wound closure and tissue repair rate of 83.04 ± 0.89%, along with a noteworthy decrease in tissue oxidative stress indicators. Histological investigations have verified the wound-healing properties of M. oleifera bark extract. Conclusion: Due to its significant antioxidant properties and its capacity to create a moist environment for wounds, M. oleifera has the potential to serve as a natural treatment for burns. Additional clinical trials are recommended to validate the efficacy of M. oleifera bark extract as a therapeutic agent for wound healing.

Effects of parecoxib sodium on early cognitive impairment and inflammation levels in burned rats.

To study the effect of parecoxib sodium in alleviating inflammation in burned rats and restoring cognitive function in burned rats. 30 SPF grade SD rats were randomly divided into 6 groups: (1) Blank control group (Group C). (2) Sham surgery group (Group Sham). (3) Second-degree burn model (Group B). (4) Low-dose (1mg/kg/d) parecoxib sodium (Group L+B). (5) Medium-dose (10mg/kg/d) parecoxib sodium (Group M+B). (6) High-dose (20mg/kg/d) parecoxib sodium (Group H+B). ELISA measures inflammatory factor IL-2, IL-6, TNF-α and IFN-γ, cognitive function factor NSE, cortisol and S-100β. Combined with water maze and dark avoidance experiments to further verify the recovery of cognitive function in rats. The contents of IL-2, TNF-α and IL-6 in Group M+B were significantly lower than those in Group Sham (P<0.05), and the content of IFN-γ was significantly lower than that in Group Sham (P<0.05). The cognitive markers NSE, S-100β and cortisol levels in Group M+B were significantly higher than those in Group Sham at 2h, 1d, 5d and 10d after operation (P<0.05). In the Group M+B dark-avoidance experiment, the number of probes and errors were not significantly different than those in Group Sham and Group C (P>0.05), and the number of times Group M+B found a platform in the water maze experiment and crossed the platform was second only to Group B and Group C. Parecoxib sodium can effectively reduce inflammation in burn rats and promote cognitive recovery in burn rats, and the optimal dose of parecoxib sodium for burn rats is 10mg/kg.

Exploring the potential mechanisms of the ethyl acetate fraction of Hippophae rhamnoides L. seeds as a natural healing agent for wound repair

Ethnopharmacological relevanceSea buckthorn (Hippophae rhamnoides L.) has been designated a "medicine food homology" fruit by the National Health Commission of China due to its nutritional value. In traditional Chinese ethnomedicine, Hippophae rhamnoides L. is commonly used to treat nonhealing wounds such as burns, sores, and gastric ulcers. The aim of this study was to explore the healing effects of the ethyl acetate extract of sea buckthorn seeds (SBS-EF) on burn wounds. Aim of the studyThe primary objectives of this research were to determine the most effective medicinal site of action for treating burns with sea buckthorn seeds (SBS) and to investigate the underlying material basis and mechanisms of their therapeutic effects. Materials and methodsThe effects of different components of SBS-EF on the proliferation and migration of human skin fibroblasts (HSFs) were evaluated via MTT assays, scratch assays, transwell assays, and hydroxyproline secretion analysis. SBS-EF displayed the greatest activity amongst the extracts. Subsequent analyses included network pharmacology methodology, molecular docking studies, ultraperformance liquid chromatography UPLC-Orbitrap-Exploris-120-MS and a severe second-degree burn rat model to investigate the chemical constituents and potential therapeutic mechanisms of the SBS-EF. ResultsIn vitro studies demonstrated the efficacy of SBS-EF in promoting HSF growth and migration. UPLC-Orbitrap-Exploris-120-MS analysis revealed that SBS-EF had ten major constituents, with flavonoids being the predominant compounds, especially catechin, quercetin, and kaempferol derivatives. Network pharmacology and molecular docking analyses indicated that SBS-EF may exert its healing effects by modulating the Wnt/β-catenin signalling pathway. Subsequent in vivo experiments demonstrated that SBS-EF accelerated burn wound healing in rats, increased hydroxyproline expression in skin tissue, facilitated skin structure repair, and enhanced collagen production and organisation over a 21 d period. Additionally, exposure to SBS-EF upregulated WNT3a and β-catenin while downregulating GSK-3β levels in rat skin tissue. ConclusionsThe wound healing properties of SBS-EF were attributed to its ability to enhance HSF growth and migration, increase hydroxyproline levels in the skin, promote collagen accumulation, reduce scarring, and decrease the skin water content. SBS-EF may also provide therapeutic benefits for burns by modulating the Wnt/β-catenin signalling pathway, as evidenced by its effective site and likely mechanism of action in the treatment of burned rats.

The effect of platelet-rich plasma and sodium alginate hydrogel on corneal wound healing after corneal alkali burns in rats with computer-assisted anterior segment optical coherence tomography image analysis

Our objective was to determine the effect of a semi-synthetic sodium alginate hydrogel and its combination with platelet-rich plasma (PRP) on histopathological, biochemical, clinical, and anterior segment optical coherence tomography (AS-OCT) data. Alkali chemical burn of the cornea was induced. Injured rats were randomly divided into five equal groups and topically treated with phosphate-buffered saline (sham), platelet-rich plasma (PRP), 0.5% sodium citrate, a semi-synthetic sodium alginate hydrogel, or a combination of PRP and hydrogel (combined group) three times daily. The degree of corneal opacity (CO), corneal epithelial staining (CES), percentage of corneal epithelial defects (CEDP), degree of ciliary hyperemia (CH), neovascularization size (NVS), and extent of neovascularization (NVE) were evaluated. AS-OCT was performed at nine days, and then rats were sacrificed. Histological examination and enzyme-linked immunosorbent assays were performed to detect the concentrations of IL-1β and MMP-9 in the cornea. There were no significant differences between the groups regarding CEDP, CO, CES, CH, NVS, or NVE on the first day after corneal alkali burn injury (p > 0,05). At the last examination, CO was significantly lower in the PRP group than in the sham group (p = 0,044), while the CO concentrations were similar in terms of NVS (p > 0,05). Similarly, in terms of tissue MMP-9 levels, there were no significant differences between groups (p > 0,05). However, there was a significant difference in tissue IL-1β levels between the groups (p < 0,001). In the PRP and combined groups, the level of IL-1β was significantly lower than that in the sham group (p = 0,043 and p = 0,036, respectively). There was a significant difference in epithelial necrosis between the PRP, and it was the lowest in the combined group (p = 0,003). Epithelial thickness was highest in the combined group (p = 0,002). CEDP was significantly different at the last visit between the groups (p = 0.042). The fastest epithelial closing rate was observed for the combined group (p = 0,026). There was a significant negative correlation between tissue MMP-9 levels and corneal solidity and between tissue MMP-9 levels and the corneal area according to the AS-OCT measurements (p = 0,012 and p = 0,027, respectively). When used alone, topical hydrogel application did not significantly enhance the healing of corneal wounds. However, when combined with PRP, it leads to an increased rate of epithelial closure and neovascularization. This combination did not exacerbate inflammation or corneal opacity compared to PRP alone. The anticoagulant citrate solution in the PRP tube did not prove effective. The synergistic use of PRP and hydrogel could enhance epithelial thickness and reduce epithelial necrosis. The use of new parameters for corneal wound healing assessment was facilitated through AS-OCT image processing.

Dexmedetomidine on the interplay of IL-6 and STAT3 pathways in adrenal gland damage-induced scalding burns in rats.

Scalding burns are a common form of thermal injury that often leads to systemic complications. Pro-inflammatory cytokines like interleukin-6 (IL-6) and the activation of signal transducer and activator of transcription 3 (STAT3) pathways have been linked to the pathophysiology of organ damage caused by burns. This study aimed to investigate the potential therapeutic effects of dexmedetomidine, an α2-adrenergic receptor agonist with anti-inflammatory properties, on the interplay of IL-6 and STAT3 pathways in adrenal gland damage following scalding burns in rats. Twenty-eight rats were divided randomly into four groups. Rats in group 1 (n=7, control) were given only 0.9% intraperitoneal (i.p.) NaCl. Rats in group 2 (n=7, DEX) were exposed to 25°C water for 17 s on day 1 and received 100 mcg/kg/day dexmedetomidine i.p. for 3 days; for rats in group 3 (n=7, Burn), boiling water of 94°C was applied inside for 17 s. Rats in group 4 (n=7, Burn+DEX) were exposed to 94°C water for 17 s and received 100 mcg/kg/day dexmedetomidine i.p. for 3 days. Adrenal gland tissues were histopathological examined, and STAT3, IL-6, and TUNEL staining were performed using immunohistochemically. Our results revealed that scalding burns increased IL-6 and STAT3 expression in the adrenal glands of rats. Histological analysis demonstrated that dexmedetomidine administration ameliorated adrenal gland damage and reduced inflammatory cell infiltration. Our findings suggest that dexmedetomidine protects the adrenal glands in scalding burns. This protection appears to be mediated, at least in part, by its modulation of IL-6 and STAT3 pathways.

Influence of gel matrix on the wound healing activity of adhesive dressings filled with silver nanoparticles and humic acid applied on a rat burn model

BACKGROUND: Thermal injuries to the skin (burns) are common household and man-made traumatic injuries in humans. In wartime, thermal injuries are additional damaging factors in combined skin lesions caused by the damaging elements of modern weapons. AIM: This study aimed to evaluate the effectiveness of gels based on silver nanoparticles in combination with humic acid on the healing of thermal skin injuries (burn wounds) in rats. MATERIALS AND METHODS: In the experiments on 57 white nonlinear male rats weighing 218–260 g, the wound healing effects of gels with 10% humic acid modified with nanosilver on various bases, prepared in a pharmacopoeial manner, on a grade IIIB thermal skin burn model were examined. The gel bases used in three experimental groups were as follows: sodium carboxymethylcellulose (group Ia, n = 12), polyacrylamide (group Ib, n = 12), and polyethylene glycol (group Ic, n = 12). The comparison group (group II, n = 12) received Levomekol ointment, whereas the control group (group III, n = 8) did not receive treatment. A grade III thermal burn was made on the proximal part of the back under general anesthesia using a thermal applicator with a circular-shaped flat working part with a diameter of 20 mm. Seventy-two hours after the burn, the wound was freed from scab by complete excision along the border of the intact skin, a splinting ring was applied, and the study drugs were administered. The dynamics of the changes in the burn wound were assessed on days 10, 14, 17, 21, 24, and 28 using the Universal Desktop Ruler program. RESULTS: The average times (median) to reach 75% epithelialization of wounds in groups Ia, Ic, and II were 29.4 ± 0.2, 34 (33–34), and 33.3 ± 0.45 days, respectively, whereas in groups Ib and III, the median time to 75% epithelization was not achieved. The 28-day healing index (%/day) values were 8.6, 4.8, 6.5, 6.1, and 4.4 in groups Ia, Ib, Ic, II, and III, respectively. The dynamics of epithelialization of thermal burns were most pronounced in group Ia, where the sodium carboxymethylcellulose acted as a gel base for humic substances modified with nanosilver. CONCLUSIONS: The results indicated that the gel added with nanosilver-modified humic substances based on sodium carboxymethylcellulose has a wound healing effect on thermal skin injuries, superior to the effect of the reference drug Levomekol in terms of the healing time of burn wounds.

The Effect of Giving Mangrove Leaf Extract (Rhizophora apiculata) on the Healing of Burn Wounds in Male White Rats (Rattus novergicus) of the Sprague Dawley Strain

A burn wound is damage or loss of tissues caused by contact with a heat source and can be classified into four degrees: I, IIA, IIB, and III. Anti-inflammatory and antioxidant agents such as mangrove leaves can be applied to aid the healing process of burns. One alternative method is to use mangrove leaf extract. This study aims to understand the acceleration of burn healing by applying mangrove leaf extract (Rhizophora apiculata). This study was a true experimental study with a post-test-only control group design, divided into five groups: Knormal (without treatment), K+ (bioplacenton), P1 (20% extract), P2 (30% extract), and P3 (40% extract). Burn wounds were observed for 21 days and assessed with average wound shortening, healing time, and the Bates-Jensen wound assessment tool. Based on the results of statistical tests, it was found that the application of Rhizophora apiculata leaf extract affected the healing of burns in white rats, resulting in a reduction in the burn area (P Value: 0.001). The application of mangrove leaf extract had an effect on the healing time of burns in white rats (P Value: 0.001). An effective dose of mangrove leaf extract was a concentration of 40% for healing burns, a higher effectiveness in healing burns compared to the standard drug, bioplacenton. The administration of mangrove leaf extract positively affects the healing of burns in male white rats (Rattus novergicus) of the Sprague Dawley strain, especially with an extract concentration of 40%.

Regulating Tissue Growth Factors for Healing with Etherified Carboxymethylcellulose Matrix.

Etherified Carboxymethylcellulose Matrix (eCMC) is a revolutionary application of carboxymethylcellulose (CMC) in wound care, known for its potential in hemostasis and tissue regeneration. This study aims to investigate the mechanism of eCMC in tissue healing by establishing a rat burn model and administering eCMC as a treatment. The objective is to analyze cytokines and inflammatory mediators using a Cytokine Array and histochemical staining to understand the effects of eCMC on tissue regeneration. A rat burn model was created, and eCMC was applied as a treatment. Tissue samples were collected at multiple time points to assess the expression of cytokines and inflammatory mediators using a Cytokine Array. Additionally, histochemical staining was performed to evaluate tissue regeneration factors. eCMC induced the expression of endogenous cytokines, particularly VEGF and PDGF, while inhibiting inflammatory cytokines such as CINC-1, CINC-2, and MMP-8. This dual action facilitated wound healing and mitigated the risk of infection. eCMC demonstrates promising potential for enhancing skin regeneration. Further research is warranted to delve into the precise mechanism of eCMC's cytokine regulation. In vitro and in vivo studies should be conducted to comprehensively investigate the therapeutic capabilities of eCMC in wound healing.

Computational Drug Discovery in Diaphragm Dysfunction via Text Mining and Biomedical Database.

The diaphragm, which is crucial for ventilation, is the primary muscle responsible for inspiration. Patients with severe burns who experience diaphragmatic dysfunction have an increased risk of mortality. Unfortunately, there are currently no effective medications available to prevent or treat this condition. The objective of our study is to utilize bioinformatics to identify potential genes and drugs associated with diaphragmatic dysfunction. In this study, text-mining techniques were utilized to identify genes associated with diaphragmatic dysfunction and recovery. Common genes were then analyzed using GO and KEGG pathway analysis, as well as protein-protein interaction network analysis. The obtained hub genes were processed using Cytoscape software, and their expression levels in diaphragmatic dysfunction were validated using quantitative real-time polymerase chain reaction (qRT-PCR) in severe burn rats. Genes that were confirmed were then examined in drug-gene interaction databases to identify potential drugs associated with these genes. Our analysis revealed 96 genes that were common to both the "diaphragm dysfunction" and "functional recovery" text mining concepts. Gene enrichment analysis identified 19 genes representing 10 pathways. qRT-PCR showed a significant increase in expression levels of 13 genes, including CCL2, CCL3, CD4, EGF, HGF, IFNG, IGF1, IL17A, IL6, LEP, PTGS2, TGFB1, and TNF, in samples with diaphragmatic dysfunction. Additionally, we found that a total of 56 drugs targeted 5 potential genes. These findings provide new insights into the development of more effective drugs for treating diaphragmatic dysfunction and also present substantial opportunities for researching new target pharmacology and promoting drugs in the pharmaceutical industry.

Pharmaceutical composition of 1,2,4-trioxolanes with betulin in fish oil. Effect on oxidative and energy metabolism in the treatment of burns in rats

Background. Ozonation products of unsaturated fatty acids in fats and oils – 1,2,4-trioxolanes – which contribute to the normalization of oxidative and energy metabolism in various diseases, may be a promising substance for the creation of new combined anti-burn drugs.The aim. To study the effect of 1,2,4-trioxolanes in a pharmaceutical composition with betulin, which exhibits wound-healing properties, on indicators of oxidative stress and on energy metabolism in the treatment of burns in an experiment onrats. Methods. The experiments were carried out on 50 male Wistar rats with seconddegree skin burns. We assessed the activity of glutathione reductase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, aldehyde dehydrogenase, superoxide dismutase and catalase, as well as the level of lipid peroxidation indicators.Results. The properties of 1,2,4-trioxolanes were studied and a pharmaceutical composition of 1,2,4-trioxolanes with betulin from birch bark in fish oil was developed. The effect of this composition on oxidative and energy metabolism in rats during the treatment of burns was studied. The activation of NADP/H and NAD/H dependent enzymes (glutathione reductase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, aldehyde dehydrogenase), as well as superoxide dismutase and catalase, and the normalization of lipid peroxidation parameters under the influence of the studied composition in the treatment of burns were revealed. The effect of the composition on the energy metabolism of erythrocytes was noted, which was assessed by changes in the ratio of lactate dehydrogenase activity in the reverse and direct reactions. Morphohistological studies showed that using the composition under normal conditions and in case of a burn had no effect on the structure of the stomach wall, and also improved the condition of the burn wound on the day 10 compared to the wound condition in animals without treatment.Conclusion. The proposed composition of 1,2,4-trioxolanes and betulin in fish oil, which allows normalizing oxidative and energy metabolism and improving the condition of a burn wound without toxic effects on the stomach wall, can be used as an element of additional therapy in the treatment of burns.

Comparison of burn and incision wound healing in rat given crude extract of porcupine quill (Hystrix sp)

Comparison of burn and incision wound healing in rat given crude extract of porcupine quill (Hystrix sp)

Superoxide dismutase-contained solid lipid nanoparticles: Formulation development and In-vivo evaluation for second-degree burn wound healing in rats

IntroductionSuperoxide dismutase (SOD), a natural enzyme with high antioxidant activity, reduces injury and accelerates wound healing by scavenging superoxide radicals. This enzyme plays an important role in cellular defense against oxidative stress such as burn injury. The aim of this study was to load SOD into solid lipid nanoparticles for the treatment of rat burn wounds. MethodsSolid lipid nanoparticles were prepared by Solvent Emulsification Diffusion method and evaluated for particle size, enzyme activity and enzyme entrapment efficiency. Twenty-seven rats in 3 different groups were induced with deep second-degree burns and then treated with SOD-loaded solid lipid nanoparticles, solid lipid nanoparticles without enzyme, or SOD solution. After the treatment period, the wounds were evaluated macroscopically for the area of healing and microscopically for indices of re-epithelialization, granulation tissue and angiogenesis. ResultsThe optimized SOD-loaded solid lipid nanoparticles showed a particle size of 35–85 ± 2.41 nm, 78.4 ± 4.31 % entrapment efficiency and 90 % initial enzyme activity. Macroscopic examination showed that the best recovery rate belonged to the solid lipid nanoparticle group. Pathological studies also showed that angiogenesis and granulation tissue were significantly better in this group. Compared to the other two groups, SOD-loaded solid lipid nanoparticles showed a significant improvement in pathological factors, particularly angiogenesis and granulation tissue, as well as a faster reduction in the number of inflammatory cells. ConclusionBased on this study, solid lipid nanoparticles could be used as an effective delivery system for SOD in the treatment of second-degree burns.

Wound Healing Effects of Liposomal Nanocurcumin and PL Pro Nanocurcumin on Thermal Burn and Skin Ulcer

Background Burn injuries and skin ulcers are important health problems resulting in physical and psychological scars and chronic disabilities. This study investigated the wound-healing effects of liposomal nanocurcumin and PL pro nanocurcumin on thermal burns in rats and doxorubicin-induced skin ulcers in mice and their systemic toxicity. Methods Having subjected to a cylindrical hot steel rod onto the dorsum, burned lesions were covered topically with silver sulfadiazine/liposomal nanocurcumin/PL pro nanocurcumin twice a day for 21 days. Besides, the other skin lesions which were induced by a single intradermal injection of doxorubicin on the dorsal region were topically administered with dimethyl sulfoxide/liposomal nanocurcumin/PL pro nanocurcumin twice a day for 21 days. Results The results indicated that liposomal nanocurcumin and PL pro nanocurcumin significantly reduced the wound size, increased the hydroxyproline content in animals’ skin, and improved the histopathological structure of the affected tissues. Specifically, liposomal nanocurcumin demonstrated better healing results than PL pro nanocurcumin on thermal burns. Furthermore, topical administration of liposomal and PL pro nanocurcumin was deemed not to exert any systemic toxicity to the wounded animals by not influencing considerably the hematological parameters and renal and hepatic functions and altering the histology of the liver and kidney. Additionally, liposomal nanocurcumin and PL pro nanocurcumin with average sizes of 206 nm and 344 nm were well-dispersed in water, accentuating that the disadvantages of limited water solubility have been overcome. Conclusion Thus, liposomal nanocurcumin and PL pro nanocurcumin exerted effective effects on burned wounds and skin ulcers whilst triggering no systemic toxicity in wounded animals.

Healing Effect of Hydroalcoholic Extracts of Thymbra Spicata and Ferulago Angulata on Second-degree Burn Wounds in Rats

Objective: Burns can be physically and mentally painful, making them one of the worst experiences. In this study, hydroalcoholic extracts of Thymbra spicata and Ferulago angulata were investigated as treatments for second-degree burns in male Wistar rats. Methods: To create the burn wounds, a circular metal tool with a diameter of 2 cm was used, followed by treatment with prepared ointments including Ferulago angulata (1%), Thymbra spicata (1%), Thymbra spicata plus Ferulago angulata (1%), and Adib derm ointment. The wound area was measured and the wound tissue was examined histopathologically on days 1, 7, 14, and 21 in each group. The ointments were applied every day for 21 days to determine the wound healing rate. Results: The experiment showed that on the sixth and eleventh days, the groups treated with a combination of Ferulago angulata and Thymbra spicata, and Ferulago angulata alone, demonstrated significant reductions in wound area. According to histopathology, both Ferulago angulata alone and in combination with Thymbra spicata significantly improved the inflammatory condition, epithelialization process, fibroplasia, arrangement, and density of collagen fibers when compared to the other groups. Conclusion: The study showed that Ferulago angulata extract, either on its own or in combination with Thymbra spicata extract, has significant healing and restorative effects on seconddegree burn wounds in animal models.

Fermented Polyherbal Formulation Ameliorates the Severity of Acute Multiple Antibiotics-Resistant Pseudomonas aeruginosa-Infected Burn Wound in a Rat Burn Model.

Pseudomonas aeruginosa, a Gram-negative opportunistic bacterium, has emerged as a cause of life-threatening infections in burn wounds. Current therapeutic approaches through wound dressings and systemic medicines are far from satisfactory; resistance to more than two antibiotics shown by pathogens contributes to failures of therapy causing mortality. This animal study was conducted to check the efficacy of one Ayurveda-based fermented polyherbal preparation (AP 01) against multiple antibiotics-resistant (MAR) P. aeruginosa HW01-infected rat burn wounds. AP-01 was applied on artificially infected burn wound on a rat model infected with MAR P. aeruginosa to register the healing effects in terms of reduction in residual wound area percentage, the presence of C-reactive protein in blood, and the presence of viable bacteria colony. Topical application with conventional antibiotics served as a positive control. The polyherbal preparation had reduced the infected residual burn wound area at 40.63% ± 0.69 from the initial burn wound area within two weeks after a single intervention, whereas residual burn wound area remained much higher in the case of animals left untreated and in the case of the animals treated with control drug. Restoration to the normalcy of serum C-reactive protein level was also achieved earlier in the case of polyherbal AP-01-treated groups than in other groups. Fermented formulations using components of AP-01 singly or in different combinations had never been tested earlier for topical application in infected burn wound. The formulation of AP-01 was found superior in terms of the rate of healing and control of infection by MAR P. aeruginosa strains in burn wounds in rat models.

The Effect of Melatonin on Platelet Levels in Wistar Rat After Burns in Two Days

&lt;strong&gt;Background &lt;/strong&gt;: Burns can caused by high temperatures. Burns have an impact on platelet levels and hemostatic regulation. Melatonin is a therapeutic agent that can increase platelet levels on burns by neutralizing Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) so it can suppress tissue damage due to burns. This study aims to determine the effect of giving melatonin on the platelet levels of burnt wistar rats in two days&lt;em&gt;&lt;/em&gt;&lt;p&gt;&lt;strong&gt;Methods &lt;/strong&gt;: This is experimental study with a sample of 12 Wistar rats that matched the inclusion and exclusion criteria. Rats were divided into two groups, namely K1 as control and K2 as a group that was given melatonin at a dose of 10 mg/kg. Platelet levels were measured at T1 (0th hour post burn), T2 (24th hour post burn), and T3 (48th hour post burn). The data were analyzed by the Shapiro-Wilk normality test, followed by Parametric Paired t-Test and the Independent t-Test&lt;em&gt;.&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Result &lt;/strong&gt;: This experimental results there was a significant increase in the number of platelets between T2 and T3 and between T1 and T3 in control group. And in K2, there was a significant decrease in the number of platelets between T1 and T2 and between T2 and T3. Meanwhile, between T1 and T3 there was a significant increase in platelet levels.&lt;strong&gt;&lt;em&gt;&lt;/em&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion&lt;/strong&gt;: Melatonin can significantly increase the platelet levels of burn Wistar rats at 48 hours post-burn.&lt;em&gt;&lt;/em&gt;&lt;/p&gt;