Stereological Investigation of the Effects of Hypericum Perforatum L. Plant on Skin Tissue in Second Degree Burns in Rats

Abstract

The aim of this study was to examine stereologically the effect of Hypericum perforatum L. oil on skin injuries resulting from burns. Experimental groups were selected 18 healthy rats with an average weight of 250-300 grams as material. The rats were divided into 3 groups: control, burn and burn+treatment, with 6 in each group. In the control group, second-degree burns were created and the rats were perfused. Control group rats were kept in formaldehyde for a week. Burns were applied to the burn and burn+treatment groups with 100ºC water. 4gr/kg of Hypericum perforatum L. oil was given via gavage to the burn+treatment group only for 21 days. The burn group was not intervened. At the end of the process, rats in both the burn and burn+treatment groups were perfused. The skin of the animals, which were kept in formaldehyde for a week, and where second-degree burns occurred were dissected. After tissue tracking and tissue embedding procedures for all animals in the group, 5µm thick sections were taken to obtain 8-10 sections at a ratio of 1/75. Volume values were calculated using stereological methods from the sections photographed with an x4 objective. Calculations were made using the Shtereom I program in accordance with the Cavalieri Principle. Non-parametric tests and Kruskal Wallis test were used in statistical evaluations. When the results were analyzed, it was observed that the administration of Hypericum Perforatum L. oil by gavage had an effect on the healing process of second degree burn wounds.