Abstract
Purpose: To investigate the healing effect of Paris polyphylla extract (PPE) on second-degree burns in rats. Methods : Male Sprague Dawley (SD) rats, weighing 200 – 220 g, were subjected to deep seconddegree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) second-degree burn model (control) group, (2) burn model treated with 1 % silver sulfadiazine (SSD) group, and (3) burn model treated with 120 mg·mL -1 PPE group. On days 3, 7 and 14 following the administration of the drug/extract, wound area and histopathological changes in rat epidermis were evaluated for the three groups. The minimum inhibitory concentration (MIC) of PPE on Staphyloccocus aureus , Pseudomonas aeruginosa and Escherichia coli were also assessed. Results: On day 14, the mean wound area of PPE treatment group (0.21 ± 0.04 cm 2 ) was significantly smaller than that of the control rats (2.78 ± 0.18 cm 2 , p < 0.01). Histological results indicate that inflammatory cells disappeared and were replaced by new granulation tissue in the group treated with 120 mg·mL -1 PPE by day 14. Compared with SSD group rats, the inflammatory cells and fibroblast and granulation tissues of burnt rats with burns and treated with 120 mg·mL -1 PPE decreased significantly. The antibacterial data revealed that the MIC of PPE against S. aureus , P. aeruginosa and E. coli was 2.35, 8.2 and 4.70 mg·mL -1 , respectively. Conclusion: Paris polyphylla is an effective medicinal herb that holds promise for the treatment of second-degree burns. Keywords: Paris polyphylla, Second-degree burns, Wound healing, Antibacterial, Inflammatory cells, Granulation tissues, Silver sulfadiazine
Highlights
Every year, millions of people suffer major disabilities or even death from burns, caused by hot water, flame and boiling oil
The average wound area of polyphylla extract (PPE) and silver sulfadiazine (SSD) treatment groups healed more quickly than the control groups by day 14 (p < 0.01, Figure 1), suggesting that both treatment probably accelerated the process of wound healing, there was a significant reduction in the wound area
On day 0, collagen fiber was necrotic, inflammatory cells infiltrated below the striated muscles and vascular engorgement and necrosis were seen in the burn skin of model rats
Summary
Millions of people suffer major disabilities or even death from burns, caused by hot water, flame and boiling oil. People suffer from burns due to domestic and industrial accidents, which along with enormous cost of treatment, cause mortality and considerable morbidity [1]. According to the World Health Organization (WHO), there were 300,000 deaths worldwide due to burns in 2012, with 96 % of these deaths occurring in developing countries [2]. Burns are one of the health problems in modern societies associated with irreparable damage to patients and family relationships [3]. SSD is the most used topical treatment for burns due to its potent antimicrobial efficacy. It was necessary to find more efficient agents with fewer side effects for treatment of burns
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