Abstract
Purpose: To investigate the healing effect of Sanguisorba officinalis L. extract (SOLE) on seconddegree burns in rats. Methods: Male Sprague Dawley rats, weighing 200 – 220 g, were subjected to deep second-degree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) second-degree burns model (control), (2) burns model treated with 1 % silver sulfadiazine (SSD), and (3) burns model treated with SOLE. On days 3, 7 and 14, following administration of drug/extract, the wound area and histopathological changes of rat epidermis were evaluated in all the groups. Minimum inhibitory concentration (MIC) of SOLE on Staphylococcus aureus , Pseudomonas aeruginosa and Escherichia coli was also assessed separately. Results: On day 14, the mean wound area of SOLE treatment group (0.22 ± 0.05 cm 2 ) was significantly smaller than that of control rats (2.67 ± 0.18 cm 2 , p < 0.01). Histological data indicate that inflammatory cells of burnt rats disappeared and were replaced by new granulation tissue by day 14 for the group treated with SOLE. The antibacterial results revealed that the MIC of SOLE for Staphylococcus aureus , Pseudomonas aeruginosa and Escherichia coli was 3.5, 14.0 and 7.0 mg·mL -1 , respectively. Conclusion: Sanguisorba officinalis L. appears to be an effective medicinal herb for the treatment of second-degree burns. Keywords: Sanguisorba officinalis , Burns, Healing, Antibacterial, Silver sulfadiazine
Highlights
Every year, millions of people suffer major disability or even death from burns caused by hot water, flame and boiling oil
The wound area of rats treated with silver sulfadiazine (SSD) or Sanguisorba officinalis L. extract (SOLE) decreased progressively
The average wound area of SOLE and SSD treatment groups resolved more quickly than control groups by day 14 (p < 0.01), suggesting that both treatment probably accelerated the process of wound healing there was significant reduction in the wound area
Summary
Millions of people suffer major disability or even death from burns caused by hot water, flame and boiling oil. Sanguisorba officinalis L. extract was investigated on the healing activity on burn wounds in rats. On days 3, 7 and 14 following treatment, average wound areas of control, SSD and SOLE group rats were measured respectively. The top of electrical scald instrument (Changhai Hospital of Second Military Medical University, China) was pressed on the back skin with a certain force for 15 s at 75 oC In this way, the second-degree burn model was prepared in rats. Wound skin tissue samples were taken by a scalpel from the control, SSD and SOLE groups on days 7 and 14 for histological observation. On day 0, collagen fiber was necrotic, inflammatory cell infiltrated below striated muscles and vascular engorgement and necrosis were seen in the burn skin of model rats. On day 7, there was severe infiltration of inflammatory cells and some fibroblast and granulation tissues were found in the burnt skin of control rats
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