This study presents experimental results on the seismic behavior of a large-scale two-bay, two-story RC frame with RC infill walls, including openings typical of low-rise RC row buildings in Taiwan. The test findings indicated that the specimen failed due to the failure of the vertical members of the first story. Specifically, the critical wall segments located between openings and between an opening and a boundary column and short columns resulting from the presence of these openings were shown to be the main contributors to the failure. Based on the observed phenomenon during the test, an analytical model was verified and proposed for the tested specimen’s pushover analysis. The selection of plastic hinges in the analytical model was determined according to the Taiwan Earthquake Assessment for Structures by Pushover Analysis (TEASPA). Pushover analysis performed by commercially available software indicated that the analytical model could well capture the specimen's behavior regarding peak base shear, initial stiffness, displacement of each story, and development of plastic hinges.