Breeding Activity Research Articles

Racial status of Orobanche cumana Wallr. in some countries other the world

Abstract The study represents a review of the evolution of the parasitic plant Orobanche cumana Wallr. and current racial status in some sunflower cultivating countries. Identification of racial status of broomrape populations belonging to eight sunflower cultivating countries from Europe and Asia (Republic of Moldova, Romania, Bulgaria, Ukraine, Spain, Turkey, Serbia and China) revealed the presence of high virulent races G and H in the majority of countries, except Serbia, where accessions belonging to race E or less virulent than E were found. The most virulent race H was identified particularly in the Black Sea area (Romania, Ukraine and Turkey). Additionally, the emergence of new biotypes characterized by high virulence, overcoming the resistance genes to race H was observed in some areas of the Republic of Moldova, Romania and Turkey, which signifies the importance of periodic evaluations of racial status for the prevention of occurrence and dissemination of new races. Analyzing the occurrence chronology of broomrape races in different countries we conclude that by the mid-1990s, the state of play was relatively stable, the most aggressive race being E. After this period the process of O. cumana development and appearance of new more aggressive and virulent biotypes became faster, which may be explained by the intensification of sunflower breeding activities and, respectively by the selection pressure exerted on the parasite by new resistant hybrids.

Association between Phenotypic and Genotypic Distance Matrices of Advanced Breeding Lines of Lowland Rice (Oryza sativa L.) from Diverse Parents

Background: The major staple food of the world, rice, is known for preferences in dietary diversification among the communities. Evaluation of advanced breeding lines developed from a set of diverse parents involved in different cross combination facilitates selection of promising genotypes, resource management and planning further breeding activities.
 Methods: Totally, 22 advanced breeding lines of lowland rice in F7 were evaluated based on agro-morphologically and genotypically with molecular markers. Here we report correspondence between different distance matrices subjected to correlation based on Mantel test.
 Results: Non significant (p-value > 0.05) correlation was observed between the matrices due to causal factors such as data type, markers used and genetic properties of lines as explained in the text. However, certain promising lines were selected from phenotypic performance as well as molecular fingerprints and grain quality evaluation relative to checks (CAUR1 and Shasarang) with specific context to north eastern hill region.

Effect of Gamma Ray Radiation 60Co Generation M1 on Growth and Production of Cayenne Pepper (Capsicum frutescens L) Prentul Kediri Variety

Prentul cayenne pepper is a local chili in Kediri which is widely cultivated by local farmers, the continuity of chili needs to be maintained by increasing productivity. This can be overcome by improving local varieties through plant breeding activities. This study aims to determine the effect of radiation and obtain the optimal dose on the growth and production of cayenne pepper plants in the M1 generation. This research was carried out with the single plant method and using the T test, with a radiation dose of 100 Gy to 1000 Gy, and without a radiation dose or 0 Gy. Observation parameters included plant growth percentage, plant height, number of leaves, leaf width, flowering age and fruit weight. The results showed that the LD50 value of chili (Capsicum anuum L) was 572,917 Gy. The irradiation dose of 100 Gy gave the best effect on the growth and production of chili plants which had a plant height of 83.85 cm, a number of 35 leaves, a faster flowering age of 63 DAP and a fruit weight of 10.26. Gamma radiation affects plant growth. Different results were shown in the phenotypic appearance of leaf shape and fruit size. Meanwhile, at a dose of 300 Gy, it gave an effect that caused the plants to become stunted and have a longer harvest age, with the highest diversity value based on the standard deviation value of 23.93.

Evaluation of the collection material of tomato varieties in dagestan in order to identify the most promising forms for tomato breeding

Relevance. Tomato is one of the main vegetable crops and is grown everywhere in the open and protected ground in the North Caucasus region of Russia. Therefore, increasing the production of tomato fruits in the open ground is an important task for vegetable growers in the south of our country. There is too much dependence on imported seeds. To get rid of this, it is necessary to increase breeding activity, create domestic varieties and hybrids of tomatoes with high potential productivity, resistant to adverse environmental factors and adapted to local growing conditions.Methods. The research was carried out at the experimental site of the Federal Agrarian Scientific Center of the Republic of Dagestan, located in the Primorsky lowland, at an altitude of 17 m above sea level. Agrotechnics is generally accepted in the Republic of Dagestan for tomato culture.Results. The results of the research indicate that most of the studied varieties and hybrids belong to plants of the determinant type of early maturation with average stability, with large and medium fruits of flat-rounded and rounded shape, with medium and high yields and relative resistance to fungal diseases. According to the largest number of fruits on the first brush, four varieties differ: Topaz, Yana, Chelnok, Talisman (6.5-5.7). On the basis of the "number of fruits" on the second brush, almost all varieties are marked, which is of interest to breeding. On the basis of the "distance between the brushes" varieties Yana, Marti, Grant, Perst, Topaz – are characterized by a generative (shortened internodes) type of plant (12-16 cm). The source material also differs in the shape of the fruit – 8 samples have a rounded, 3 samples have an elongated oval, 5 samples have a flatrounded and 2 samples have a cuboid shape of the fruit. The color of the fruits in the technical phase of ripeness in the studied varieties and lines was with (8 samples) or without (12 samples) an intense green spot. In biological ripeness 12 varieties and lines have a red color of the fruit.

Effect of seasonal changes on the innate immunity of wild Pseudois nayaur: potential reason for its endangerment.

Seasonal climate change may impose stress on mammal's immune system, but this has never been connected to wildlife endangerment reasons. This study focuses on Pseudois nayaur, a rare Chinese sheep facing a severe situation of disappearing. Wild Pseudois nayaurs in Sichuan, China were observed in this research by an integrated method including habitat environment, foraging rules, and breeding activities investigation in ecology filed, accompanied with immunity research by peripheral blood, including erythrocyte, neutrophils, natural killer cells, and macrophages immunity by means of erythrocyte rosette, phagocytosis, lactate dehydrogenase release and acid alpha-naphthyl acetate esterase method, respectively. The results show that Pseudois nayaurs's innate and red blood cell immunity in autumn and winter Pseudois nayaurs and it may be the results of energy reallocation between reproduction and the immune system. In addition, the environment pressure found in Zhu Balong, Sichuan, China including food reduction, human disturbance, low temperature, and severe drought still existed, which may prevent the Pseudois nayaur from normal immunity enhancement before winter. In conclusion, the innate immunosuppression found in this study especially at their breeding season in autumn and winter may be the ignored cause of wild Pseudois nayaur's endangerment and should be considered in their protection strategy.

Correlations among morphological traits of sweet chestnut (Castanea sativa Miller) from Bosnia and Herzegovina

Abstract Sweet chestnut forests in Bosnia and Herzegovina are underrepresented in the total forest fund, and their area is declining today. The research aims to determine the correlation relations of morphological traits of chestnut leaves, fruit and cupule in the population of Bosnian Krajina (northwestern Bosnia and Herzegovina) to undertake selection and breeding activities. Eleven traits of fruits, leaves and cupules were measured, seven assessed and six calculated. A mutual correlation for the researched traits was calculated using the Pearson correlation coefficient r. When the fruit traits and cupule traits were compared, the needle length showed a statistically significant correlation with most of the fruit traits. The traits of the number of fruits in the cupule and needle length had a statistically significant correlation coefficient with leaf blade width and leaf petiole length. The correlated traits can be distinguished as important for further selection and breeding of sweet chestnuts. The research results indicate that the genetic material of sweet chestnut is a rich source of genetic diversity and can be used in selection to obtain new varieties and cultivars in Bosnia and Herzegovina and for the protection of the indigenous gene pool.

BUDAYA DAN KEARIFAN LOKAL DALAM PENGELOLAAN LAHAN DENGAN POLA AGROFORESTRI DI KESATUAN PENGELOLAAN HUTAN LINDUNG BATUTEGI PROVINSI LAMPUNG

Agroforestry is a land management system widely applied in forest land utilization by incorporating forestry and agricultural crops. In its management, farmers have their way that is influenced by the local culture and wisdom inherited by their parents. Therefore, this research aims to identify farmers local culture and wisdom in land management with agroforestry patterns. Data retrieval has been conducted in February-March 2021 with case studies in 3 combined farmer groups (Gapoktan), namely Gapoktan Hijau Makmur, Gapoktan Mahardika, and Gapoktan Cempaka which are, in the protected forest management unit (KPHL) Batutegi, Lampung Province. Data collection is conducted with structured interviews and in-depth interviews. In this study it was identified that the way the culture of ngoret influences farmers land management, angin-anginan, nunas, perantingan, and setek on plant maintenance, mutil and ngunduh on harvesting and breeding activities. In addition, this culture also contains wisdom in babat belukar on the preparation of land and nandur on planting. This local wisdom is still applied in a limited way so that it needs to be developed to be widely applied to support forest sustainability in KPHL Batutegi.Key words: Breeding, Crop maintenance, Harvesting, Land preparation, Planting.

Initial Trial Test of Potential Yield on 22 New Lines of Maize (Zea mays. L)

Corn (Zea mays L.) is an important food ingredient as one of the second carbohydrate producers after rice in Indonesia. The use of the corn plant is not only limited to the seeds, but other parts of the corn plant can also be used. To increase national maize production, intensification methods can be applied. One of the intensification activities can be realized by selecting planting materials obtained from crossing. The superior hybrid seeds can be obtained from intensive plant development and breeding activities. The purpose of this study was to determine the yield potential and characteristics of the new hybrid lines on the maize plant being tested. The research was conducted from January 2019 to May 2019 on an experimental plot owned by PT. BISI International Tbk. in Sambirejo Village, Bendo District, Kediri Regency. The design used in this experiment was a randomized block design with 2 replications. The number of treatments used consisted of 25 treatments with 22 new lines collected by PT. BISI International Tbk. and 3 comparison varieties such as BISI 18, NK6172 and P35. Observational variables observed included plant height (cm), plant vigor, flowering age of male plants (DAS), flowering age of female plants (DAS), harvest age (DAS), ear length (cm), moisture content (%), potential yield (tonnes / ha), seed yield (%), number of rows per ear, number of seeds per row, ear diameter (cm), percentage of seed filling (%), weight of 1000 seeds (g) and density (g / ml). The prospective hybrid candidates that have higher yield components than other hybrid lines are H6 and H11. The hopeful hybrid candidate excels in the potential outcome observation variables from the observed data.

Evaluation of the frost tolerance of Hungarian-bred walnut cultivars

At present very few walnut cultivars can be said to be cosmopolitan cultivars, grown widely in the walnut-producing countries of the world. Walnut (Juglans regia L.) has poor ecological adaptability, as its cultivation is greatly influenced by low temperatures during the winter dormancy period and in early spring. The breeding activities conducted in various countries are therefore of great significance. Choosing suitable locations for cultivation is of key importance if optimum yield stability is to be achieved. The introduction of foreign walnut cultivars regularly runs into problems if, despite their high yielding ability, they are unable to adapt to the Hungarian climate. In Hungary the most critical weather events for walnuts are the frequent frosts in early spring. Buds therefore need to burst late to avoid damage to the flowers. Many papers have dealt with the frost tolerance of stone fruit, but only limited information has been published on the frost tolerance of walnut. For the first time in Hungary, artificial freezing tests were performed in the present work to determine the frost tolerance of the cultivars available in Hungary. The results could be of service to Hungarian growers in choosing the most suitable cultivar.

Evaluation of the frost tolerance of Hungarian-bred walnut cultivars

At present very few walnut cultivars can be said to be cosmopolitan cultivars, grown widely in the walnut-producing countries of the world. Walnut (Juglans regia L.) has poor ecological adaptability, as its cultivation is greatly influenced by low temperatures during the winter dormancy period and in early spring. The breeding activities conducted in various countries are therefore of great significance. Choosing suitable locations for cultivation is of key importance if optimum yield stability is to be achieved. The introduction of foreign walnut cultivars regularly runs into problems if, despite their high yielding ability, they are unable to adapt to the Hungarian climate. In Hungary the most critical weather events for walnuts are the frequent frosts in early spring. Buds therefore need to burst late to avoid damage to the flowers. Many papers have dealt with the frost tolerance of stone fruit, but only limited information has been published on the frost tolerance of walnut. For the first time in Hungary, artificial freezing tests were performed in the present work to determine the frost tolerance of the cultivars available in Hungary. The results could be of service to Hungarian growers in choosing the most suitable cultivar.

Reproductive Biology and Population Dynamics of Indian Gerbil Rat Tatera indica in Wheat Crop

Tatera indica, the Indian gerbil rat trapped during different growth stages of wheat crop, revealed that maximum mature gerbils (80-100%) were trapped during later growth stages (panicle initiation preharvest + lean period). While, 25 and 50% pregnant ones as well as 100 and 75% cyclic gerbils were collected during dough and preharvest stages, respectively. The weight of ovaries (13.00-19.00 mg/100 gbw), number and diameter of various follicles such as primordial (13.00-16.75; 19.57-19.98 μm), primary (6.50-7.33; 93.12-96.07 μm), secondary (2.75-3.67; 204.42-216.95 μm), pre-antral (2.75-3.50; 277.63-332.57 μm), antral (3.25-3.67; 474.44-499.98 μm), atretic (3.00-3.50; 211.49-247.33 μm) and corpus luteum (3.50- 5.50; 622.24-629.76 μm) were significantly more during later growth stages i.e. dough, preharvest stages and lean period. The sperm motility (89.50-89.67%), viability (92.50-92.67%) and count (119.67-131.75 millions/ml) was significantly more during dough and preharvest stages. The diameter of seminiferous tubules (203.68- 229.44 μm), number of spermatogonia (16.67-17.50), elongating spermatids (85.67-88.50) and elongated spermatids (81.00- 89.50) were significantly more during panicle initiation, dough and preharvest stages. Thus, control operations of T. indica should be done during tillering/panicle initiation stage before the initiation of their peak breeding activity during dough/ preharvest stages

Seeds Phenotypes Characterization of Promising Tungro-Resistant Rice Lines

Breeding activities to produce a new site-specific rice varieties resistant to tungro require of suitable genetic materials as cross-breeding materials. This research aims to determine the seed phenotypic characters of promising tungro resistant rice lines and their similarity. The research was conducted using a completely randomized design with treatment of 42 promising tungro-resistant rice lines, 1 resistant rice variety (Inpari 7) and 1 susceptible rice variety (TN1), that each of which was repeated 3 times. The characters were observed included of quantitative and qualitative characters. Observation of seed characters was carried out based on the standard system of characterization and evaluation of rice plants. The similarity of seed characters was analyzed by using the NTSYS pc version 2.1 program and displayed in the form of a dendrogram. The results showed that most of the tested lines had the same quantitative characters as the Inpari 7 variety. The value of variability of diversity was included in the broad category of >4, with the lowest similarity coefficient of 63% and the highest of 94%. The similarity analysis based on the seed characters resulted in 2 large groups consisting of 41 lines belonging to the Inpari 7 group and 1 line belonging to the TN1 group. The lines that were incorporated in the Inpari 7 group were further divided into two subgroups of 39 and 2 lines, respectively. The results of this research are preliminary data to obtain an information of the relationship between seed characters and tungro resistance properties.

Favorable spring conditions can buffer the impact of winter carryover effects on a key breeding decision in an Arctic-breeding seabird.

The availability and investment of energy among successive life‐history stages is a key feature of carryover effects. In migratory organisms, examining how both winter and spring experiences carryover to affect breeding activity is difficult due to the challenges in tracking individuals through these periods without impacting their behavior, thereby biasing results.Using common eiders Somateria mollissima, we examined whether spring conditions at an Arctic breeding colony (East Bay Island, Nunavut, Canada) can buffer the impacts of winter temperatures on body mass and breeding decisions in birds that winter at different locations (Nuuk and Disko Bay, Greenland, and Newfoundland, Canada; assessed by analyzing stable isotopes of 13‐carbon in winter‐grown claw samples). Specifically, we used path analysis to examine how wintering and spring environmental conditions interact to affect breeding propensity (a key reproductive decision influencing lifetime fitness in female eiders) within the contexts of the timing of colony arrival, pre‐breeding body mass (body condition), and a physiological proxy for foraging effort (baseline corticosterone).We demonstrate that warmer winter temperatures predicted lower body mass at arrival to the nesting colony, whereas warmer spring temperatures predicted earlier arrival dates and higher arrival body mass. Both higher body mass and earlier arrival dates of eider hens increased the probability that birds would initiate laying (i.e., higher breeding propensity). However, variation in baseline corticosterone was not linked to either winter or spring temperatures, and it had no additional downstream effects on breeding propensity.Overall, we demonstrate that favorable pre‐breeding conditions in Arctic‐breeding common eiders can compensate for the impact that unfavorable wintering conditions can have on breeding investment, perhaps due to greater access to foraging areas prior to laying.

Conservation of genetic resources for sustainable aquaculture

Conservation of genetic resources for sustainable aquaculture

Population structure and dynamics, breeding activity and phenology of the blue‐sided treefrog (Agalychnis annae)

Abstract Agalychnis annae is a species endemic to Costa Rica and Panama with populations shrinking or disappearing from several locations. We studied the reproduction of a relict population of A. annae in a 1,300 m2 natural pond in Costa Rica during 2007–2008. We marked adults with a visible implant alphanumeric tag injected in the thigh. We measured reproductive effort considering calling males per night and per hour, number of pairs in amplexus, and number of egg masses. We summed 366 individual observations: 57% juveniles, 39% adult males, and 4% adult females. Average snout–vent length was 29 mm (juveniles), 63 mm (males), and 78 mm (females). Sex ratio (males:females) was 4.85:1, while operational sex ratio varied between one and six males per female when both sexes were present at the pond. Maximum estimated census population size varied between 27 and 59 individuals, with a Ne = 43.11. Calling activity occurred mostly from 1800–2000 h, and we counted 65 egg masses. Number of adults and calling males were positively correlated with accumulated rainfall and average temperature. Number of egg masses was positively correlated with the number of adults, accumulated rainfall, and average temperature. The causes of the long‐term population decline must be identified to conserve A. annae and similar species, as well as establishing long‐term monitoring programs. Required conservation actions include translocation individuals from risky areas, creation of artificial ponds to increase ecological connectivity, elimination of introduced species, particularly in small habitat remnants, and establishment of ex situ conservation programs.Abstract in Spanish is available with online material.

Development and Validation of a 36K SNP Array for Radiata Pine (Pinus radiata D.Don)

Radiata pine (Pinus radiata D.Don) is one of the world’s most domesticated pines and a key economic species in New Zealand. Thus, the development of genomic resources for radiata pine has been a high priority for both research and commercial breeding. Leveraging off a previously developed exome capture panel, we tested the performance of 438,744 single nucleotide polymorphisms (SNPs) on a screening array (NZPRAD01) and then selected 36,285 SNPs for a final genotyping array (NZPRAD02). These SNPs aligned to 15,372 scaffolds from the Pinus taeda L. v. 1.01e assembly, and 20,039 contigs from the radiata pine transcriptome assembly. The genotyping array was tested on more than 8000 samples, including material from archival progenitors, current breeding trials, nursery material, clonal lines, and material from Australia. Our analyses indicate that the array is performing well, with sample call rates greater than 98% and a sample reproducibility of 99.9%. Genotyping in two linkage mapping families indicated that the SNPs are well distributed across the 12 linkage groups. Using genotypic data from this array, we were also able to differentiate representatives of the five recognized provenances of radiata pine, Año Nuevo, Monterey, Cambria, Cedros and Guadalupe. Furthermore, principal component analysis of genotyped trees revealed clear patterns of population structure, with the primary axis of variation driven by provenance ancestry and the secondary axis reflecting breeding activities. This represents the first commercial use of genomics in a radiata pine breeding program.

Sistem Pakar Mendiagnosa Penyakit Ayam Kampung Menggunakan Metode Certainty Factor

Animal husbandry is the activity of breeding and raising livestock to obtain the benefits and results of these activities. Activities in the field of animal husbandry can be divided into two groups, namely livestock farming of large animals such as cows, buffaloes and horses, while the second group is farming of small animals such as chickens, ducks, birds and others. Village chicken farming activities have existed since ancient times. Village chickens start from the first generation, namely from the descendants of the red jungle fowl (Gallus gallus). Raising free-range chickens is not easy because there are many things that must be considered and must have problems, therefore an expert system is needed to assist farmers in diagnosing and knowing the type of disease and symptoms experienced by the free-range chicken using a computer system automatically. This expert system uses the Certainty Factor method which aims to measure the level of certainty of facts or rules to describe an expert's belief in the problem at hand. This study shows how the process of calculating the Certainty Factor method in diagnosing free-range chicken disease with the initial combination rule to the last combination rule based on the selected symptoms, it can be concluded that the highest weight value is D01 Avian Influenza with a weight value of 0.99 x 100% = 99%

NESTING OF THE YUCATAN VIREO, VIREO MAGISTER, IN MAINLAND MEXICO AND ON ISLA COZUMEL, WITH DETAILS OF BREEDING PHENOLOGY

We report the first definitive Mexican breeding records of the range-restricted Yucatan Vireo (Vireo magister). We discovered two V. magister nests in July and August 2009 in Sian Ka’an Biosphere Reserve (near Tulum) and one nest on Isla Cozumel in June 2009. We observed two possible additional V. magister nests with eggs in Sian Ka’an in 2009, one each in June and July. All nests were open cups woven onto branches and hung beneath forks. Clutch size was two eggs at definitive V. magister nests. Nests and eggs were similar to those of most Vireo spp., including sympatric nesting congeners. Breeding activities—including egg-laying, incubation, and nestling and post-fledging provisioning—at definite Yucatan Vireo nests occurred in June and July on Cozumel and July and August on the mainland. Incubation and nestling stages lasted ~ 15 days and ~ 14 days, respectively. Yucatan Vireo remains poorly known, warranting further study.

Genetic diversity, population structure and relationship of Ethiopian barley (Hordeum vulgare L.) landraces as revealed by SSR markers

Evaluation and characterization of genetic resources maintained at both in situ and ex situ GenBanks have important implications for future utilization in association mapping studies, genetic selection, breeding and conservation activities. The main objective of this study was to evaluate the genetic diversity, population structure and relationship of 384 Ethiopian barley genotypes collected from different barley growing regions of Ethiopia using 49 simple sequence repeat (SSR) markers. Analysis of these 49 SSR markers amplified a total of 478 alleles with an average of 9.755 alleles per locus were obtained of which 97.07% of the loci were observed to be polymorphic. Nei's genetic diversity index (h) was 0.654 and the Shannon diversity index (I) was 0.647, indicating that the genetic diversity in barley genotypes studied was moderately high. At the population level, mean per cent of polymorphic loci (PPL) showed 98.37%, h = 0.388 and I = 0.568. Highest level of genetic diversity was observed in the Arsi population (with PPL = 100%, h = 0.439, I = 0.624); the lowest value observed was in population from Jimma (with PPL = 75.51%, h = 0.291, I = 0.430). Analysis of molecular variance (AMOVA) result showed significant genetic differentiation within and between populations (P<0.001), with 84.21% and 15.79% of the variation occurring within and among populations, respectively. Further, genetic variation analysis showed a coefficient of gene differentiation of 0.053 and a gene flow value of 4.467 among populations. The 384 barley genotypes were divided into seven genetic clusters according to STRUCTURE, neighbour-joining tree and principal coordinate analysis, correlating significantly with geographic distribution. These results signified presence of significant variations within and among populations (P<0.001), with the highest of the variation occurring within populations. The results will also assist with the design of conservation strategies, such as genetic protection via both in situ and ex situ conservation.

Applicability of inter-primer binding site iPBS- retrotransposon marker system for the assessment of genetic diversity and population structure of Peruvian rosewood (Aniba rosaeodora Ducke) germplasm

Rosewood (Aniba rosaeodora Ducke), which has a great demand due to its essential oil globally, is an evergreen tree of the Amazon forests. Rosewood natural stands have been depleted through deforestation and the destruction of habitat. Currently, rosewood is included in the ICUN red list of endangered species. The 11 highly polymorphic primers amplified total 305 bands of which 301 (98.69%) were polymorphic. The number of effective alleles (Ne), Shannon's information index (I), overall gene diversity (Ht), gene diversity (h), and polymorphism information content (PIC) were (1.562), (0.505), (0.330), (0.337) and (0.343), respectively. These diversity indices explored high genetic diversity in rosewood germplasm. Among studied germplasm, the Santa Marta population was found most diverse by reflecting higher values of diversity indices while the Zungarococha population was found least diverse. The analysis of molecular variance (AMOVA) revealed that 79% of the genetic variation was within the populations. The STRUCTURE algorithm, unweighted pair group with arithmetic mean (UPGMA), and principal coordinate's analysis (PCoA) separated all germplasms into different population groups according to their geographic locations. Santa Marta population was found more diverse by reflecting higher values of diversity indices. The maximum genetic distance (0.868) was found between the Huajoya-10 and Nanay-3. In this investigation, iPBS- retrotransposon marker system was used to explore the genetic diversity of Peruvian rosewood germplasm. The results in this study such as higher genetic diversity indices, AMOVA (79%) within population and PIC value (0.343) showed the utility and reproducibility of iPBS-retrotransposons in this species successfully. The STRUCTURE algorithm separated the germplasms into six population groups according to their geographic locations. These results have valuable information for the conservation, management strategies and future breeding activities of rosewood.