We investigate the Casimir effect of a rough membrane within the framework of the Hořava–Lifshitz theory in 2+1\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$2+1$$\\end{document} dimensions. Quantum fluctuations are induced by an anisotropic scalar field subject to Dirichlet boundary conditions. We implement a coordinate transformation to render the membrane completely flat, treating the remaining terms associated with roughness as a potential. The spectrum is obtained through perturbation theory and regularized using the ζ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\zeta $$\\end{document}-function method. We present an explicit example of a membrane with periodic border. Additionally, we consider the effect of temperature. Our findings reveal that the Casimir energy and force depend on roughness, the anisotropic scaling factor and temperature.