Bone Tissue Grafts Research Articles

A 3D micro-CT assessment of composition and structure of bone tissue after vertical and horizontal alveolar ridge augmentation using CAD/CAM-customized titanium mesh.

To date, no studies have exploited micro-CT in humans to evaluate bone morphology and structure after bone augmentation with CAD/CAM-customized titanium mesh, in mandible and maxilla. The aim of this study was to assess the composition and microstructure of bone biopsy through micro-CT analysis. Bone augmentation at both maxillary and mandible sites was performed on 30 patients randomly treated with customized mesh, either alone (M-) or covered with resorbable membrane (M+), in both cases filled 50:50 with autogenous bone and xenograft. After 6 months, biopsies were taken and micro-CT was performed on consecutive 1-mm-thick VOIs from coronal to apical side, measuring tissue volumes, trabecular thickness, spacing, and number. In both groups, irrespective of membrane use, bone tissue (M-: 29.76% vs. M+: 30.84%) and residual graft material (M-: 14.87% vs. M+: 13.11%) values were similar. Differences were site-related (maxillary vs. mandibular) with higher percentage of bone tissue and trabecular density of low-mineralized bone and overall bone in the mandible. The composition and structure of bone tissue, as assessed by micro-CT after alveolar ridge augmentation using CAD/CAM-customized titanium meshes, showed similar features regardless of whether a collagen membrane was applied.

Antibacterial sequential growth factor delivery from alginate/gelatin methacryloyl microspheres for bone regeneration

Autologous or allogeneic bone tissue grafts remain the mainstay of treatment for clinical bone defects. However, the risk of infection and donor scarcity in bone grafting pose challenges to the process. Therefore, the development of excellent biomaterial grafts is of great clinical importance for the repair of bone defects. In this study, we used gas-assisted microfluidics to construct double-cross-linked hydrogel microspheres with good biological function based on the ionic cross-linking of Cu2+ with alginate and photo-cross-linking of gelatin methacryloylamide (GelMA) by loading vascular endothelial growth factor (VEGF) and His-tagged bone morphogenetic protein-2 (BMP2) (AGMP@VEGF&BMP2). The Cu2+ component in the microspheres showed good antibacterial and drug-release behavior, whereas VEGF and BMP2 effectively promoted angiogenesis and bone tissue repair. In in vitro and in vivo experiments, the dual cross-linked hydrogel microspheres showed good biological function and biocompatibility. These results demonstrate that AGMP@VEGF&BMP2 microspheres could be used as a bone defect graft substitute to promote effective healing of bone defects and may be applied to other tissue engineering studies.

CURRENT POSSIBILITIES AND PROSPECTS OF ALVEOLAR BONE DEFECT REPLACEMENT AND COVERING TISSUES: NARRATIVE LITERATURE REVIEW

BACKGROUND: The percentage of the need to increase the volume of supporting tissues remains high - from 43% to 77%, despite the fact that the currently used approaches for augmentation of tissue structures do not always show the expected results. A common cause of problems with the growth of new supporting tissues is the reduced activity of inducing factors of local and systemic levels in the human body. A critical deficiency of the integumentary structures significantly affects the result of reconstructive and reconstructive surgical interventions.Objective: to characterize the modern achievements of intraoral reconstruction of volumetric defects of supporting tissues, including bioengineering for the regeneration of the alveolar jaw bone and integumentary structures.Methods. The search strategy included an electronic search of literature sources performed in the PubMed database using keywords. The formats "Review", "Systematic Review" and "Clinical Trial" were requested. The search depth was 20 years.Results. Of the 378 articles found, 44 met the inclusion criteria set for this review. The prerequisites for the reconstruction of the alveolar ridge with an emphasis on early vascularization of de novo tissues are outlined, the advantages and disadvantages of bone and soft tissue grafts are characterized. The results of research efforts using mesenchymal stem cells, which play a crucial role in the regeneration of the alveolar bone and gum, are presented. The evolution of tissue engineering structures for integumentary tissues is presented – from thin layers of epithelial cells to 3D – epithelized equivalents of the oral mucosa.Conclusion. The rapidly increasing number of biomaterial studies involving stem cells, the active development of tissue engineering indicates the prospects for the development of biomaterials of the next generations, which will be able to work effectively together with their own mechanisms of tissue regeneration in their unique architecture.

Effects of high antibiotic concentrations applied to continuous local antibiotic perfusion on human bone tissue-derived cells.

Continuous local antibiotic perfusion (CLAP) has recently attracted attention as a new drug delivery system for orthopaedic infections. CLAP is a direct continuous infusion of high-concentration gentamicin (1,200 μg/ml) into the bone marrow. As it is a new system, its influence on the bone marrow is unknown. This study aimed to examine the effects of high-concentration antibiotics on human bone tissue-derived cells. Cells were isolated from the bone tissue grafts collected from six patients using the Reamer-Irrigator-Aspirator system, and exposed to different gentamicin concentrations. Live cells rate, apoptosis rate, alkaline phosphatase (ALP) activity, expression of osteoblast-related genes, mineralization potential, and restoration of cell viability and ALP activity were examined by in vitro studies. The live cells rate (the ratio of total number of cells in the well plate to the absorbance-measured number of live cells) was significantly decreased at ≥ 500 μg/ml of gentamicin on day 14; apoptosis rate was significantly increased at ≥ 750 μg/ml, and ALP activity was significantly decreased at ≥ 750 μg/ml. Real-time reverse transcription-polymerase chain reaction results showed no significant decrease in the ALP and activating transcription factor 4 transcript levels at ≥ 1,000 μg/ml on day 7. Mineralization potential was significantly decreased at all concentrations. Restoration of cell viability was significantly decreased at 750 and 1,000 μg/ml on day 21 and at 500 μg/ml on day 28, and ALP activity was significantly decreased at 500 μg/ml on day 28. Our findings suggest that the exposure concentration and duration of antibiotic administration during CLAP could affect cell functions. However, further in vivo studies are needed to determine the optimal dose in a clinical setting.

The Use of Tissue Grafts Associated with Immediate Implant Placement to Achieve Better Peri-Implant Stability and Efficacy: A Systematic Review and Meta-Analysis

Background: The goal of this systematic study and meta-analysis was to evaluate the efficacy of hard and/or soft tissue grafts associated with type-1 implants on healing and treatment outcomes. The primary outcomes studied were implant survival rate, pocket depth, marginal peri-implant recession, bone loss, bone thickness (volumetric change), interproximal bone level, mesial and distal papilla migration, and radiographic evaluation; and the secondary parameters were Pink Esthetic Score (PES), vertical distance from implant shoulder and bone, Visual Analogue Score (VAS), Implant Stability Quotient (ISQ), and biological complications (fistulas, pain, mucositis, and peri-implantitis). Methods: The PICO strategy was used to formulate the hypothesis under study: “For patients who underwent extraction and immediate implant placement, what is the efficacy of using any type of graft (bone or soft tissue) compared to non-grafting regarding the peri-implant parameters?” The electronic search process was performed on the MedLine/PubMed and Cochrane databases. It included randomized controlled trials (RCTs) from the last 11 years (from 2012 to November 2023), which were identified and analyzed. Results: Nine RCTs (κ = 0.98) were selected (403 patients and 425 implants); they were divided into three groups: bone graft (75 patients and 75 implants inserted), bone graft and membrane (213 patients and 235 implants inserted), and without bone graft (115 patients and 115 implants inserted). Three studies calculated the mid-facial mucosa level and two reported better results when a connective tissue graft was combined with the xenograft, whereas another study found better results in the combination of a dual-zone technique with a xenograft. Three studies evaluated the total Pink Esthetic Score (PES) at 12 months, where the authors found no significant difference in using a xenogeneic graft with or without a membrane. In the same period, the facial bone thickness was assessed in two articles; the authors reported better results in graft-treated and flapless groups. The risk-of-bias assessment found four studies with low risk, four with moderate risk, and one with a high risk of bias. The meta-analysis showed a medium level of heterogeneity for the mid-facial mucosa level analysis (I2 = 46%) and an overall effect size of 0.79 (95% CI [0.18; 1.40]), a statistically significant results (p = 0.01), with a tendency to favor the experimental group. Also, there was a medium level of heterogeneity among studies regarding total PES (I2 = 45%), with no significant differences between studies (p = 0.91). Homogeneous results (I2 = 0%) were found among studies analyzing facial bone thickness, favoring the experimental group; the forest plot showed an effect of 0.37 (95% CI [0.25; 0.50]), which was statistically significant (p < 0.00001) for this parameter. Conclusions: Then, it was possible to conclude that using bone and soft tissue grafting techniques associated with immediate implant placement (IIP), even though they are not fundamental, was a valuable resource to prevent significant tissue reduction, reaching greater bone stability and higher levels in the Pink Esthetic Score (PES) and Visual Analogue Score (VAS).

Evaluation of inherent properties of the carboxymethyl cellulose (CMC) for potential application in tissue engineering focusing on bone regeneration

AbstractBiomaterials are essential in medicine because these biological macromolecules have appropriately replaced classical tissue grafting techniques for their valuable features. Bone tissue engineering has persistently developed since “tissue engineering” was suggested. Carboxymethyl cellulose (CMC) is the first FDA‐approved water‐soluble derivative of cellulose that could be targeted for desired bone tissue graft. Numerous studies on CMC as a component created for bone tissue have recently been published. Because of its carboxylate groups, CMC is hydrophilic. CMC can crosslink with varied materials, such as synthetic and natural polymers, enabling innovative bone structure biomaterials. These carboxylate groups are responsible for in situ gelations and bio‐adhesion characteristics. In this review, the current progress and inherent characteristics of CMC‐based bone scaffold materials are discussed.

VISTA approach in conjunction with enamel matrix derivative, cortico-cancellous bone, and connective tissue graft for periodontal defect surgery: A case series.

The biggest challenge during periodontal regeneration in the anterior region is the prevention of soft-tissue recession. Minimally invasive surgeries, particularly papilla preservation techniques and soft-tissue augmentation, may significantly reduce such postoperative soft-tissue recession. This article presents the vestibular incision subperiosteal tunnel access approach for periodontal regeneration in the anterior region. A subperiosteal tunnel prepared from a single vertical vestibular incision adjacent to the defect is used for debridement, enamel matrix derivative application, defect grafting with cortico-cancellous tuberosity bone, and insertion of the connective tissue graft. Evaluation of six cases with up to 6 years of follow-up showed improvements in all clinical parameters. The probing pocket depth improved from 8.2±0.75 mm initially to 2.7±0.52 mm at follow-up, clinical attachment level improved from 8.5±0.83 mm initially to 2.7±0.52 mm at follow-up, and midfacial gingival recession of 1 mm at two sites was corrected. The papillae were stable at all sites, with an average distance of 4.8 mm from the incisal edge to the papilla tip. This technique seems to be a promising approach for achieving both esthetic and functional goals of periodontal regenerative surgery. However, experience in performing microsurgeries and harvesting tuberosity tissues may be a limitation.

Periodontal phenotype modification in orthodontic patients.

The purpose of this study was to assess the feasibility of phenotype modification in orthodontic patients using combined bone and soft tissue grafting substitutes. The surgical procedure was conducted on 18 patients (3 males, 15 females). Periodontal phenotype modification was conducted using demineralized freeze-dried bone allograft and a xenogeneic collagen matrix. The following parameters were recorded for each tooth at baseline and 12-month follow-up: O'Leary plaque index (PI), probing depth (PD), bleeding on probing (BOP), gingival thickness (GT), keratinized tissue width (KTW), gingival recession (GR), and vestibular depth (VD). The results showed a statistically significant increase in GT (2.02 ± 0.39 mm), KTW (1.11 ± 0.82 mm), and VD (0.18 ± 1.16 mm) (p < 0.05). GR was also significantly decreased (1.02 ± 0.99 mm) (p < 0.05). Within the limitation of this study, the proposed approach enhanced the periodontal condition in orthodontic patients. However, further studies with a larger sample size are needed to ensure long-term stability. Hard and soft tissue conditions have paramount importance for long-term periodontal stability. Phenotype modification in orthodontic patients can diminish the probability of adverse consequences and result in optimal esthetic outcomes. The proposed technique using combined bone and soft tissue substitutes indicated promising results and could be recommended in orthodontic patients with thin periodontal phenotypes.

CAD/CAM Titanium Meshes for GBR: A Case Series with Preliminary Histologic Analysis.

Titanium has been proposed as a mesh material for guided bone regeneration (GBR) since the 1990s. To overcome difficulties in shaping and adapting meshes to the defect, digital techniques were introduced to digitally print meshes capable of fitting the bone perfectly, reproduced through the patient's CT scan. Five patients were included in this case series, and their CBCT data were acquired and sent to the producer of the titanium meshes. 3D regenerative surgery was performed with titanium meshes and a mix of demineralized bovine bone matrix (DBBM) and autogenous bone (1:1 ratio). Radiographic measures were evaluated on paraxial sections of the CBCT through a dedicated software. When possible, regenerated bone samples were obtained at implant insertion. Four out of five regenerated areas healed without local or systemic complications. One mesh was removed after 2 months and 2 weeks due to exposure. The mean vertical bone gain was 4.3 ± 1.5 mm (range: 2.5 to 7 mm). Two histologic samples were obtained. In sample 1, bone tissue area and graft material area were 44.4% and 12.5%, respectively; in sample 2, the same parameters were 15.6% and 16.9%, respectively.

Human Bone-Marrow-Derived Stem-Cell-Seeded 3D Chitosan-Gelatin-Genipin Scaffolds Show Enhanced Extracellular Matrix Mineralization When Cultured under a Perfusion Flow in Osteogenic Medium.

Tissue-engineered bone tissue grafts are a promising alternative to the more conventional use of natural donor bone grafts. However, choosing an appropriate biomaterial/scaffold to sustain cell survival, proliferation, and differentiation in a 3D environment remains one of the most critical issues in this domain. Recently, chitosan/gelatin/genipin (CGG) hybrid scaffolds have been proven as a more suitable environment to induce osteogenic commitment in undifferentiated cells when doped with graphene oxide (GO). Some concern is, however, raised towards the use of graphene and graphene-related material in medical applications. The purpose of this work was thus to check if the osteogenic potential of CGG scaffolds without added GO could be increased by improving the medium diffusion in a 3D culture of differentiating cells. To this aim, the level of extracellular matrix (ECM) mineralization was evaluated in human bone-marrow-derived stem cell (hBMSC)-seeded 3D CGG scaffolds upon culture under a perfusion flow in a dedicated custom-made bioreactor system. One week after initiating dynamic culture, histological/histochemical evaluations of CGG scaffolds were carried out to analyze the early osteogenic commitment of the culture. The analyses show the enhanced ECM mineralization of the 3D perfused culture compared to the static counterpart. The results of this investigation reveal a new perspective on more efficient clinical applications of CGG scaffolds without added GO.

Adjustable Elasticity of Anatomically Shaped Lattice Bone Scaffold Built by Electron Beam Melting Ti6Al4V Powder

This study investigates the elasticity of specific lattice structures made from titanium alloy (Ti6Al4V), namely, anatomically shaped lattice scaffolds (ASLS) aimed for reinforcement of the bone tissue graft that substitute a missing piece of the previously injured bone during its recovery. ASLSs that were used for testing were fabricated using the Electron Beam Melting (EBM) method. The mechanical properties of the ASLS were examined through uniaxial compression tests. Compression testing revealed the complex non-linear behavior of the scaffold structure’s elasticity, with distinct compression stages and deformation dependencies. The ASLS structures exhibited quasi-elastic deformation followed by the rupture of individual struts. Results demonstrate that the ASLSs can be stiffened by applying appropriate compression load and accordingly achieve the target elasticity of the structure for the specific load range. The modulus of elasticity was determined for different compression stages of ASLS, allowing interpolation of the functional relation between the modulus of elasticity and compressive force that is used for stiffening the ASLS. This study enhances the understanding of the mechanical behavior of the specific lattice structures made of Ti6Al4V and provides insights for the development of mechanically optimized anatomically shaped lattice scaffolds.

Scaphoid Bone Nonunions: Clinical and Functional Outcomes of Collagen/PGA Scaffolds and Cell-Based Therapy.

In this study, the procedure for treating the nonunion complication of scaphoid fractures using collagen/poly glycolic acid (CPGA) scaffolds with bone marrow mesenchymal stem cell (BM-MSC) therapy was adopted and compared with the commonly employed autologous bone tissue graft. With conducting a two-armed clinical trial, 10 patients with scaphoid nonunions were enrolled in this investigation. Patients were randomly assigned to two groups treated with (1) CPGA + cell therapy and (2) autologous iliac crest bone graft standard therapy. Treatment outcomes were evaluated three months after surgery, measuring the grip and pinch strengths and wrist range of motion, with two questionnaires: Patient-Rated Wrist Evaluation (PRWE) and Quick form of Disabilities of the Arm, Shoulder, and Hand (QDASH). We have also assessed the union rate using clinical and radiologic healing criteria one and three months post-operatively. Restorative effects of CPGA + cell therapy were similar to those of the autologous bone graft standard therapy, except for the grip strength (P = 0.048) and QDASH score (P = 0.044) changes, which were higher in the CPGA + cell therapy group. Three months following the surgery, radiographic images and computed tomography (CT) scans also demonstrated that the scaphoid union rate in the test group was comparable to that of scaphoids treated with the standard autograft method. Our findings demonstrate that the CPGA + cell therapy is a potential alternative for bone grafting in the treatment of bone nonunions.

Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts

Demineralized bone matrix (DBM) is considered one of the most reliable bone tissue grafts for regular surgical use, as it provides a scaffold that is structurally like native bone, and that enhances bone regeneration. However, commercially available DBM products are not suited for surgical restitutions of large bones. Therefore, each Tissue Bank is urged to implement their own demineralization protocol, which usually does not meet the high demand for bone grafting. In this project, we developed an open source system for medium-scale manufacturing of DBM grafts from human cadaveric donors to automate the demineralization protocol and improve its reproducibility. The device consists in (1) unidirectional flow reaction chamber, where the demineralization protocol takes place; (2) automated syringe pump, which controls the reagent́s inlet and vacuum; and (3) reagent dispenser, for the management of the reagents need for the demineralization protocol. Validation of the device included histological analysis, DNA quantification temperature regulation, electrochemiluminescence and colorimetric protocols, followed by the optimization of physicochemical parameters based on Response Surface Methodology. The results showed values of residual lipids and calcium within standardized ranges, and the maintenance of the structural integrity of the DBM, demonstrating the capacity of the system to support the proposed demineralization protocol.

Use of Nanocomposites in Bone Regeneration.

This article examines the compositions of bone tissue grafting and presents tissue culture and engineering formally as an approach for orthopaedic surgery. We assessed articles on bone grafts, analyzed their properties, advantages, and restrictions, and delivered explanations on technologies, including bone-tissue engineering (BTE). Osteo graft materials range from real human bone autografts (self-grafts) to substitute materials that can be used as grafts. These can be used single-handedly or conjointly to improve bone healing and regeneration. Tissue engineering is a relatively newer and evolving alternative for reducing the challenges of bone grafts and improving the rehabilitation of bone fractures and defects. Shortly, the combination of scaffolds, healing factors, gene therapy, and, more recently, 3D printing of tissue-engineered constructs may yield new perceptions.Natural bone tissue has a nanocomposite structure that offers the right biological and physical characteristics. It is essential that the biomaterial resemble real bone tissue in order to regenerate bone tissue. Because they can offer the correct matrix environment, combine desirable biological features, and allow regulated, sequential distribution of numerous growth factors for the different phases of bone tissue regeneration, nanocomposites are the ideal alternative for bone tissue regeneration. This is because no single type of material can replicate the composition, structure, and characteristics of native bone.A relatively new class of materials called nanocomposite biomaterials combines a biopolymeric and biodegradable matrix structure with nanoscale fillers that are bioactive and easily resorbable.There are also some things to think about when using nanoparticles and nanocomposites as scaffolds in clinical settings for bone tissue engineering.

Influence of the Changes in the Bone Mineral Density on the Guided Bone Regeneration Using Bioinspired Grafts: A Systematic Review and Meta-analysis

Nowadays, developing bone tissue grafts is the key to regenerative medicine. Different materials like hydroxyapatite (HAp) have been proven to guide bone regeneration. However, their design is still lacking since most do not consider the patient's requirements. HAps can be synthetic or natural, and their Ca/P ratio varies depending on the reagents for the synthesis and the biological sources. Usually, synthetic HAps are designed to have a 1.67 Ca/P ratio, but Ca/P and Mg/P ratios naturally change throughout life for different reasons, such as age, gender, lifestyle, genetics, physical activity, body weight, and diet, accompanied by changes in bone mineral density (BMD) and bone mineral content (BMC). Until now, there is not enough information about mammal bone changes based on these ratios, BMD, BMC at different life stages, and people's gender. To face this lack of knowledge, animal models such as rats can be used to identify requirements for proper bone grafting materials based on life stages since they consider the age range and gender. Findings indicate that BMD, BMC, Ca/P, and Mg/P change as a function of the age and gender of rats. Thus, it suggests that the grafts need a personalized development considering these parameters.

A new era in fundamentals of bone homeostasis: biocompatibility of bone mineral doped fluoride ions with osteoblast cells in the balance of calcium and phosphate metabolism

Aim: The use of biocompatible bone tissue grafts, filling materials, bone minerals, and implants, particularly in medicine and dentistry studies, has expanded significantly in recent years, as have expectations from the materials. We aimed to test the biocompatibility and wound and tissue biocompatibility of many grafts and similar materials used in medicine and dentistry with tests such as cytotoxicity, scratch assay, cell adhesion, and hemolysis.&#x0D; Material and Method: In this study, the interaction of fluorine ions with a dental material was investigated by biological activity experiments. In addition, studies were carried out on important osteoblast cells for tissue regeneration control. For this process, cell migration analysis, which we do not encounter frequently in the literature, was used to examine the interaction of cells with biomaterials more sharply.&#x0D; Results: Flor ions do not create a cytotoxic effect and also increase the viability of osteoblasts which is important for tissue regeneration and are bone precursor cells.&#x0D; Conclusions: In this study, in which the efficiency of osteoblast cells was discussed, it was concluded that 2% fluorine added material had more effective biological results compared to the increase in fluorine ion ratio.

Tissue-engineered bones with adipose-derived stem cells - composite polymer for repair of bone defects.

Background: Development of alternative bone tissue graft materials based on tissue engineering technology has gradually become a research focus. Engineered bone composed of biodegradable, biosafe and bioactive materials isattractive, but also challenging. Materials & methods: An adipose-derived stem cell/poly(L-glutamic acid)/chitosan composite scaffold was further developed for construction of biodegradable and bone-promoting tissue-engineered bone. A series ofcomposite scaffold materialswith differentphysical properties such as structure, pore size, porosity and pore diameter was developed. Results: The composite scaffold showed good biodegradability and water absorption, and exhibited an excellent ability to promote bone differentiation. Conclusion: This type of biodegradable scaffold is expected to be applied to the field of bone repair or bone tissue engineering.

Research progress of in-situ three dimensional bio-printing technology for repairing bone and cartilage injuries

To review the research progress of in-situ three dimensional (3D) bio-printing technology in the repair of bone and cartilage injuries. Literature on the application of in-situ 3D bio-printing technology to repair bone and cartilage injuries at home and abroad in recent years was reviewed, analyzed, and summarized. As a new tissue engineering technology, in-situ 3D bio-printing technology is mainly applied to repair bone, cartilage, and skin tissue injuries. By combining biomaterials, bioactive substances, and cells, tissue is printed directly at the site of injury or defect. At present, the research on the technology mainly focuses on printing mode, bio-ink, and printing technology; the application research in the field of bone and cartilage mainly focuses on pre-vascularization, adjusting the composition of bio-ink, improving scaffold structure, printing technology, loading drugs, cells, and bioactive factors, so as to promote tissue injury repair. Multiple animal experiments have confirmed that in-situ 3D bio-printing technology can construct bone and cartilage tissue grafts in a real-time, rapid, and minimally invasive manner. In the future, it is necessary to continue to develop bio-inks suitable for specific tissue grafts, as well as combine with robotics, fusion imaging, and computer-aided medicine to improve printing efficiency.

Reconstruction of a single-tooth traumatic defect in the anterior maxilla using the khoury bone plate graft

Often Trauma to teeth leads to defects in the dento-alveolar process which precludes many challenges to straight forward implant therapy of the patient. Bone and soft tissue augmentation hence becomes mandatory before any implant surgery can be undertaken. This helps in adequately preparing the site for implant and its restoration. Defects in the anterior aesthetic zone that require both bone and soft tissue grafting and a restoration that harmonizes the adjacent pink and white aesthetics presents even more significant challenge to the restorative team. Hereafter a case of trauma to an anterior maxillary tooth that saw destruction of the ridge is presented, with the defect reconstructed to accommodate a functional and aesthetically pleasing implant supported restoration.

Editorial: From Passive to Active Regeneration: The New Gold Standard in Bone and Soft Tissue Grafting

Editorial: From Passive to Active Regeneration: The New Gold Standard in Bone and Soft Tissue Grafting