Blueberry Production Research Articles

Native Cultivable Bacteria from the Blueberry Microbiome as Novel Potential Biocontrol Agents.

Blueberry production is affected by fungal postharvest pathogens, including Botrytis cinerea and Alternaria alternata, the causative agents of gray mold disease and Alternaria rot, respectively. Biocontrol agents adapted to blueberries and local environments are not known to date. Here, we report on the search for and the identification of cultivable blueberry epiphytic bacteria with the potential to combat the aforementioned fungi. Native, blueberry-borne bacterial strains were isolated from a plantation in Tucumán, Argentina and classified based on 16S rRNA gene sequences. Antagonistic activities directed at B. cinerea and A. alternata were studied in vitro and in vivo. The 22 bacterial strains obtained could be attributed to eleven different genera: Rosenbergiella, Fictibacillus, Bacillus, Pseudomonas, Microbacterium, Asaia, Acinetobacter, Curtobacterium, Serratia, Sphingomonas and Xylophilus. Three strains displaying antagonistic impacts on the fungal pathogens were identified as Bacillus velezensis (BA3 and BA4) and Asaia spathodeae (BMEF1). These strains are candidates for biological control agents of local blueberry production and might provide a basis for the development of eco-friendly, sustainable alternatives to synthetic pesticides.

Pesticide risk to managed bees during blueberry pollination is primarily driven by off-farm exposures

When managed bee colonies are brought to farms for crop pollination, they can be exposed to pesticide residues. Quantifying the risk posed by these exposures can indicate which pesticides are of the greatest concern and helps focus efforts to reduce the most harmful exposures. To estimate the risk from pesticides to bees while they are pollinating blueberry fields, we sampled blueberry flowers, foraging bees, pollen collected by returning honey bee and bumble bee foragers at colonies, and wax from honey bee hives in blooming blueberry farms in southwest Michigan. We screened the samples for 261 active ingredients using a modified QuEChERS method. The most abundant pesticides were those applied by blueberry growers during blueberry bloom (e.g., fenbuconazole and methoxyfenozide). However, we also detected highly toxic pesticides not used in this crop during bloom (or other times of the season) including the insecticides chlorpyrifos, clothianidin, avermectin, thiamethoxam, and imidacloprid. Using LD50 values for contact and oral exposure to honey bees and bumble bees, we calculated the Risk Quotient (RQ) for each individual pesticide and the average sample RQ for each farm. RQ values were considered in relation to the U.S. Environmental Protection Agency acute contact level of concern (LOC, 0.4), the European Food Safety Authority (EFSA) acute contact LOC (0.2) and the EFSA chronic oral LOC (0.03). Pollen samples were most likely to exceed LOC values, with the percent of samples above EFSA’s chronic oral LOC being 0% for flowers, 3.4% for whole honey bees, 0% for whole bumble bees, 72.4% for honey bee pollen in 2018, 45.4% of honey bee pollen in 2019, 46.7% of bumble bee pollen in 2019, and 3.5% of honey bee wax samples. Average pollen sample RQ values were above the EFSA chronic LOC in 92.9% of farms in 2018 and 42.9% of farms in 2019 for honey bee collected pollen, and 46.7% of farms for bumble bee collected pollen in 2019. Landscape analyses indicated that sample RQ was positively correlated with the abundance of apple and cherry orchards located within the flight range of the bees, though this varied between bee species and landscape scale. There was no correlation with abundance of blueberry production. Our results highlight the need to mitigate pesticide risk to bees across agricultural landscapes, in addition to focusing on the impact of applications on the farms where they are applied.

Effect of container size, substrate composition, and genotype on growth and fruit quality of young southern highbush blueberry in a container-based intensive production system

Southern highbush blueberry (SHB) production is continuing to expand worldwide. Innovative horticulture practices including container-based cultivation, evergreen management, high-density planting, and high-tunnel production have been explored to maximize the productivity and profitability of SHB production. However, there is a lack of information on the feasibility of integration of all these practices to establish an intensive production system in Florida. We aimed to test and optimize this intensive system by evaluating container size, substrate composition, and genotype. Two container sizes (50.5 L and 40.4 L), five different combinations of pine bark, coco coir, and perlite, and five SHB genotypes were tested in this study. Plant vegetative growth, yield, and berry quality were measured for two years. There were no impacts of substrate composition and container size on vegetative or reproductive growth during the two-year production period studied. Plant height and canopy width varied among genotypes due to their canopy architecture. Yields of each genotype ranged from 1.0 kg/bush to 2.5 kg/bush in the first year and ranged from 0.4 kg/bush to 2.1 kg/bush in the second year. Significant differences in the performance of five genotypes tested in this study indicated that is necessary to breed cultivars specifically for this intensive system.

Seasonal Climate Trends across the Wild Blueberry Barrens of Maine, USA

Wild blueberries in Maine, USA are facing threats from our changing climate. While summer climate variations have been affecting this important commercial crop directly, significant climate variations in other seasons also can be potentially detrimental to blueberry production. Therefore, we analyzed annual and seasonal climate trends (temperature, rainfall, snow cover) over the past 41 years (1980–2020) for seven Maine counties (Piscataquis, Washington, Hancock, Knox, Lincoln, Kennebec, York) with large wild blueberry areas. We found that, across all blueberry production fields (or “barrens”), historical temperatures increased significantly (p < 0.05) in the fall and winter followed by summer, but not in the spring. Additionally, precipitation increased slightly (0.5–1.2 mm/year) in the winter and fall, whereas no changes were found in the spring and summer. Furthermore, we found that historical temperatures were lower in Piscataquis (north-central) and Washington (north-east) counties, whereas in south-western counties (Hancock to York) experienced a relatively warmer climate. The rate of increasing temperature was comparatively slower in the warmer barrens located towards the south-west (Hancock to York). Moreover, the growing season lengthened towards the fall season consistently in all locations, whereas lengthening towards the spring was inconsistent. These findings inform the wild blueberry growers in different locations of Maine about the seasonal shifts occurring for their crop. This knowledge may assist with land management planning in order for the growers to prepare for future impacts.

Fertility Effects on Blueberry Gall Midge (Diptera: Cecidomyiidae) in Wild Blueberry (Vaccinium angustifolium; Ericales: Ericaceae).

Blueberry gall midge, Dasineura oxycoccana (Johnson) (Diptera: Cecidomyiidae) is a pest in American cranberry, Vaccinium macrocarpon Aiton (Ericales: Ericaceae) and wild blueberry, Vaccinium angustifolium Aiton (Ericales: Ericaceae), and has been observed in areas of high soil and foliar nutrient levels. New management strategies, including fertilization, will need to be altered to sustain wild blueberry production under climate change and, in turn, may impact the occurrence of this pest. The purpose of this study was to measure the effect of diammonium phosphate (DAP) fertilizer application on density of infested stems and the combined effects of fertilizer application with and without infestation on wild blueberry systems. This study was conducted at two field sites in Jonesboro and Washington, ME during 2020 and 2021. Despite increases in habitat availability (blueberry cover) for the gall midge and greater infestation density, wild blueberry production benefited from DAP application. This was shown in the number of buds per stem at both sites, as well as in stem height, flowers, green fruit, blue fruit per stem, and total yield at the Washington site. Foliar nitrogen and phosphorus levels had a significant positive linear relationship with infestation density. Growers applying fertilizers should monitor blueberry gall midge field infestation levels due to our findings that DAP fertilizer impacted infestation density and the interaction of DAP fertilizer with infestation impacted wild blueberry production.

Pot and Ridge Production of Three Highbush Blueberry (Vaccinium corymbosum L.) Cultivars under High Tunnels

In recent years, new approaches to intensive blueberry (Vaccinium corymbosum L.) production have become necessary, in terms of protected environments and planting systems. These are designed to avoid numerous production difficulties, such as market saturation, damage from hailstorms, bird attacks, and spring frosts, and specific soil property requirements. Use of high tunnels and planting in a custom substrate (e.g., pots, along ridges) have gained interest among growers in recent years. As in our previous study, we determined the performance of three blueberry cultivars, ‘Duke’, ‘Aurora’, and ‘Brigitta’, when planted in pots and along a ridge under a high tunnel. Substrate water content was maintained at the same level for the pots and the ridge, although the substrate temperature fluctuations were greater for pots. Plant growth in pots was significantly lower for ‘Duke’ and ‘Aurora’ compared to the ridge. Additionally, for ‘Aurora’, the fruit yield was significantly lower for pots (103.4 g/plant), compared to the ridge (315.2 g/plant), although the opposite was seen for ‘Brigitta’ (122.4 vs. 93.5 g/plant, respectively). Individual sugar and organic acid contents mostly coincided with total contents, with lower total sugars for ‘Duke’ and higher total organic acids for ‘Aurora’ and ‘Brigitta’ for pots. For ‘Duke’ and ‘Brigitta’ fruit, the contents of some individual phenolics showed significant differences between treatments for phenolic acids and flavonols. These data show that growth in pots can be a useful planting method for the blueberry cultivars ‘Duke’ and ‘Brigitta’, and high yields and good fruit quality can be attained by following correct technological measures.

First Report of Coniella castaneicola Causing Leaf Blight on Blueberry (Vaccinium virgatum) in China.

Blueberry (Vaccinium virgatum), an economically important small fruit crop, is characterized by its highly nutritive compounds and high content and wide diversity of bioactive compounds (Miller et al. 2019). In September 2020, an unknown leaf blight disease was observed on Rabbiteye blueberry at the Agricultural Science and Technology Park of Jiangxi Agricultural University in Nanchang, China (28°45'51"N, 115°50'52"E). Disease surveys were conducted at that time, the results showed that disease incidence was 90% from a sampled population of 100 plants in the field, and this disease had not been found at other cultivation fields in Nanchang. Leaf blight disease on blueberry caused the leaves to shrivel and curl, or even fall off, which hindered floral bud development and subsequent yield potential. Symptoms of the disease initially appeared as irregular brown spots (1 to 7 mm in diameter) on the leaves, subsequently coalescing to form large irregular taupe lesions (4 to 15 mm in diameter) which became curly. As the disease progressed, irregular grey-brown and blighted lesion ran throughout the leaf lamina from leaf tip to entire leaf sheath and finally caused dieback and even shoot blight. To identify the causal agent, 15 small pieces (5 mm2) of symptomatic leaves were excised from the junction of diseased and healthy tissue, surface-sterilized in 75% ethanol solution for 30 sec and 0.1% mercuric chloride solution for 2 min, rinsed three times with sterile distilled water, and then incubated on potato dextrose agar (PDA) at 28°C for 5-7 days in darkness. Five fungal isolates showing similar morphological characteristics were obtained as pure cultures by single-spore isolation. All fungal colonies on PDA were white with sparse creeping hyphae. Pycnidia were spherical, light brown, and produced numerous conidia. Conidia were 10.60 to 20.12 × 1.98 to 3.11 µm (average 15.27 × 2.52 µm, n = 100), fusiform, sickle-shaped, light brown, without septa. Based on morphological characteristics, the fungal isolates were suspected to be Coniella castaneicola (Cui 2015). To further confirm the identity of this putative pathogen, two representative isolates LGZ2 and LGZ3 were selected for molecular identification. The internal transcribed spacer region (ITS) and large subunit (LSU) were amplified and sequenced using primers ITS1/ITS4 (Peever et al. 2004) and LROR/LR7 (Castlebury and Rossman 2002). The sequences of ITS region (GenBank accession nos. MW672530 and MW856809) showed 100% identity with accessions numbers KF564280 (576/576 bp), MW208111 (544/544 bp), MW208112 (544/544 bp) of C. castaneicola. LSU gene sequences (GenBank accession nos. MW856810 to 11) was 99.85% (1324/1326 bp, 1329/1331 bp) identical to the sequences of C. castaneicola (KY473971, KR232683 to 84). Pathogenicity was tested on three blueberry varieties ('Rabbiteye', 'Double Peak' and 'Pink Lemonade'), and four healthy young leaves of a potted blueberry of each variety with and without injury were inoculated with 20 μl suspension of prepared spores (106 conidia/mL) derived from 7-day-old cultures of LGZ2, respectively. In addition, four leaves of each variety with and without injury were sprayed with sterile distilled water as a control, respectively. The experiment was repeated three times, and all plants were incubated in a growth chamber (a 12h light and 12h dark period, 25°C, RH greater than 80%). After 4 days, all the inoculated leaves started showing disease symptoms (large irregular grey-brown lesions) as those observed in the field and there was no difference in severity recorded between the blueberry varieties, whereas the control leaves showed no symptoms. The fungus was reisolated from the inoculated leaves and confirmed as C. castaneicola by morphological and molecular identification, fulfilling Koch's postulates. To our knowledge, this is the first report of C. castaneicola causing leaf blight on blueberries in China. The discovery of this new disease and the identification of the pathogen will provide useful information for developing effective control strategies, reducing economic losses in blueberry production, and promoting the development of the blueberry industry.

Long-term Effects of Pre-plant Incorporation with Sawdust, Sawdust Mulch, and Nitrogen Fertilizer Rate on ‘Elliott’ Highbush Blueberry

Soil amendment, mulching, and fertilization practices are key components of blueberry production, yet grower practices range widely and long-term impacts are not commonly studied. ‘Elliott’ northern highbush blueberry (Vaccinium corymbosum L.) was evaluated from establishment to maturity (2003–18) to investigate the impacts of pre-plant sawdust incorporation (with or without 141 m3·ha−1 sawdust incorporated into the bed area), sawdust mulch (with or without an 8-cm-deep layer on soil surface), and N fertilizer rate (low, medium, and high, increased incrementally from 22, 67, and 112 kg·ha−1 in 2004, respectively, to 56, 168, and 269 kg·ha−1 of N from 2010 to 2018). Soil with sawdust incorporated had 4.3% soil organic matter at the end of the study in 2018 compared with 3.4% for nonincorporated soil. Soil pH was higher with sawdust incorporation and mulch when plants were young, but by 2011 these treatments were similar. High rates of N fertilization decreased soil pH by 0.3 to 0.4 throughout the study compared with the low rate, but all treatments were within or above the recommended pH range (4.5–5.5) throughout the study. Low levels of N fertilization were associated with higher soil pH and lower leaf N in most years, but higher leaf Ca and often any impacts of the low N rate were mitigated when sawdust was incorporated. Soil and leaf Ca increased when sawdust was incorporated and used as a mulch and when fertilizing with the low rate of N, but fruit Ca concentration only increased with mulch and the low N rate, whereas levels decreased with incorporation. When sawdust was not incorporated before planting, N fertilization rate affected leaf N, Ca, S, and Mn concentration, whereas this was not found when soil was amended with sawdust. Unmulched plants generally had higher leaf N, K, Fe, and Al but lower leaf Ca compared with mulched. Sawdust incorporation increased yield 4% and produced fruit with higher total soluble solids (TSS), but similar firmness, on average (2008–13), than for unamended soil. There was no main effect of mulch on yield or berry traits; however, plants grown with sawdust incorporated and no mulch had 7% greater yield per plant (averaged over 2006–13) compared with incorporated with mulch or nonincorporated with or without mulch. Nitrogen fertilization rate had no effect on yield, but berry weight was greater with low or medium N rates, particularly when sawdust was not incorporated. Net returns from higher yield with sawdust incorporation more than compensated for the materials and labor costs. Berry firmness and TSS were similar among incorporation, mulch, and fertilizer treatments for most years. Incorporating sawdust before planting resulted in an estimated $7680/ha greater net profit from fruit sales during the study period, more than compensating for the initial materials and application cost ($3150/ha). Use of the low rate of N from 2004 to 2018 saved $2680/ha and $5152/ha compared with the medium and high rates, respectively.

Economic Analysis of Southern Highbush Blueberry Production Using Drip Irrigation and Frost Protection in Georgia, USA

A partial enterprise budget simply evaluates the costs and returns profile of the agricultural firm. The simplicity in the partial enterprise budget comes with a cost that it lacks accuracy as it uses fixed single estimates and ignores potential variation in the components of the agricultural production processes. We study risk-rated returns from southern highbush blueberry production using a drip irrigation system under 5 scenarios of the blueberry prices and yields. The risk-rated return analysis gauges the returns over total costs under different specified situations addressing possible uncertainty. We show the chance of profit from southern highbush blueberry in Georgia is 69% in any full production year with the expected returns over costs of $952 per acre. This approach is helpful to minimize risk at the farmer’s production level and policy formation level.

Comparative Insecticide Application Techniques (Micro-Sprinkler) Against Drosophila suzukii Matsumura (Diptera: Drosophilidae) in Highbush Blueberry.

Drosophila suzukii (Matsumura), spotted-wing drosophila, is a major pest in small fruit crops including highbush blueberry. Controlling D. suzukii is challenging and chemical control is the main method to manage D. suzukii populations. Growers have expressed interest in using micro-sprinklers as an alternative method to apply insecticides. The current study aimed to evaluate if insecticide applications using micro-sprinklers can be used as an alternative method to protect the fruit from D. suzukii egg-laying. Modeling was used as an additional tool to parameterize the relative insecticide efficacy on oviposition. Field measurements of different treatments were conducted over periods of eleven days on commercial-standard highbush blueberry. Cyantraniliprole and spinetoram were applied using both a micro-sprinkler and a backpack sprayer. Treatments of Chromobacterium subtsugae and zeta-cypermethrin were only applied using a backpack sprayer. Both cyantraniliprole and spinetoram treatments resulted in moderate suppression of D. suzukii egg-laying. No statistical significance was found between micro-sprinkler and backpack sprayer applications for these two insecticides. Zeta-cypermethrin treatments using a backpack sprayer resulted in the most significant suppression of D. suzukii egg-laying over eleven days, while C. subtsugae was less effective at preventing D. suzukii egg-laying. Modeling simulations estimate the impact of the control methods on D. suzukii populations dynamics and simulation outputs indicated that backpack sprayers reduced D. suzukii populations at more pronounced levels compared to micro-sprinkler applications. The present study indicates that there is an underlying value of micro-sprinkler systems as an alternative and rapid spray application technique to help suppress D. suzukii pest populations during high-pressure periods in highbush blueberry production.

First Report of Bacterial Canker on Blueberry (Vaccinium corymbosum) Caused by Pseudomonas syringae pv. syringae in Serbia.

During May 2021, necrosis of young twigs and flower buds were observed on two-year-old highbush blueberry plants (Vaccinium corymbosum) cv. Draper, in a 1 hectare orchard in the municipality of Šabac, Serbia. Disease symptoms included reddish-brown to black irregularly shaped cankers developing on the shoot tips that extended downwards along the branches. In some plants, cankers surrounded the stem, causing shoot-tip dieback and necrosis of the buds. Beneath the bark, a distinct margin between diseased and healthy tissue was visible. A few weeks before symptoms development, seven freezing events with temperature from -3°C to -1°C, and five near-freezing temperatures were recorded in this area, leading to the hypothesis that symptoms were associated to the presence of ice nucleating bacteria belonging to Pseudomonas syringae. The observed disease incidence was 80%, while 10% of the plants died. Bacteria were isolated from symptomatic tissue on King's medium B (KB). After 2 to 3 days of incubation at 27°C, predominantly grey-whitish, shiny, round, convex bacterial colonies were observed on agar plates. Ten isolates producing a fluorescent pigment on KB were selected for further characterization by biochemical and molecular tests. The isolates were Gram, oxidase and arginine-dihydrolase negative, levan positive, induced hypersensitive response on tobacco leaves and showed no pectinolytic activity on potato slices. Based on the results of API 20E and API 20NE tests (BioMerieux, France), and the fact that isolates did not utilize tartrate nor had tyrosinase activity, they were preliminarly identified as Pseudomonas syrinage pv. syringae (Braun-Kiewnick and Sands 2001). Additionally, all tested isolates had ice-nucleation activity at -5°C. The syrB gene responsible for syringomycin synthesis, was amplified in all isoaltes with the specific primer pair B1/B2 (Sorensen et al. 1998). The 16S rRNA gene sequences of five selected isolates (GenBank MZ410287 to 91) showed 100% identity to P. s. pv. syringae isolated from Prunus avium in United Kingdom (GenBank CP026568) and France (GenBank LT962480). Sequences of gyrB gene (Sarkar and Guttman 2004) of two selected isolates (GenBank MZ420633 and MZ420634) showed 98,44% identity to the P. s. pv. syrinage strain isolated in France (GenBank LT962480). Pathogenicity of the isolates was confirmed on 2-year-old blueberry plants cv. Draper, by inoculating two plants per isolate. One-cm long wounds were made on branches using a scalpel and 20 µl of bacterial suspension (106 CFU/ml) was infiltrated into the tissue. The cuts were then covered with moist sterile cotton pads and wraped in parafilm for 3 days. Inoculation was also performed on two leaves per plant by needleless syringe infiltration (106 CFU/ml). Sterile distilled water was used as a negative control. Plants were maintained in greenhouse at 27°C day and 15°C night temperature. Three weeks after inoculation, the inoculated branches and leaves developed necrosis, leaves spots and cankers respectively, resembling the natural infection. Symptoms were not observed on the control plants. Bacteria were reisolated from symptomatic tissue and their identity was confirmed by amplifying the syrB gene sequence and additional biochemical tests. This is the first report of bacterial canker of highbush blueberry caused by P. s. pv. syringae in Serbia. In Europe, there was only one report on Pseudomonas spp. causing disease on blueberry leaves in Poland (Kaluzna et al. 2013). Due to market demands and export potential, blueberry production in Serbia has been rapidly increasing. In 2015, highbush blueberry was cultivated on 220 ha, while in 2020 the area increased to 1899 ha. However, under favourable environmental conditions, blueberry production might be severely affected by bacterial canker.

Biochar as an Alternative Soil Amendment for Establishment of Northern Highbush Blueberry

Biochar, as a soil amendment, has been reported to improve plant growth by increasing soil moisture and retaining nutrients. In a previous 12-week greenhouse study with highbush blueberry (Vaccinium hybrid), we found that amending soil with biochar alone or in combination with bokashi (fermented wheat bran) increased plant growth relative to unamended soil. The biochar was produced from mixed conifer species during conversion of wood to energy. In the current study, we aimed to validate the greenhouse findings under field conditions in western Oregon. The specific objectives of this 2-year study were to determine the effect of amending soil with biochar or a combination of biochar and bokashi on growth and early fruit production during establishment of northern highbush blueberry (Vaccinium corymbosum L.). To achieve these objectives, we transplanted ‘Duke’ blueberry plants into soil that was either unamended or amended with biochar or 4:1 (v/v) mixtures of biochar and bokashi or biochar and douglas fir [Pseudotsuga menziesii (Mirb.) Franco] sawdust. Each amendment was either applied in the planting hole or incorporated into the row. A treatment with douglas fir sawdust incorporated into the row was also included and represented the industry standard for the region. Plants grown in soil amended with biochar (in the planting hole or row) had 40% to 74% greater total dry weight at the end of the first growing season and 70% to 82% greater fruit yield in the second season than those grown with no amendments or in soil amended with sawdust. However, leaf Mg concentrations were lower with biochar, suggesting it could limit Mg uptake in blueberry. Soil amended with sawdust, on the other hand, was higher in organic matter, microbial activity, and wet stable aggregates than the other soil treatments but resulted in lower leaf N concentrations during the second year after planting. Unlike in the greenhouse study, biochar had no effect on root colonization by mycorrhizal fungi, and there was no benefit to using biochar with bokashi. Adding 4 L of biochar to the planting hole was considerably more economical than applying it to the row and cost $1320/ha less than the industry standard of incorporating sawdust in the row. These findings indicate that biochar is a promising soil amendment for commercial production of highbush blueberry.

Will Climate Warming Alter Biotic Stresses in Wild Lowbush Blueberries?

As global temperatures rise, a growing need exists for understanding the impacts of warming on crop production. Warming not only changes crop physiology and growth but also the weeds, insect pests, and diseases of crops including wild lowbush blueberries, which have not been studied till now. Changes in pest pressures can cause instability in production and will require changes in management practices and the development of mitigation strategies. The objective of this study was to determine the impacts of warming on the prevalence of major weeds, insect pests, and diseases of the wild blueberry production system. We selected six genotypes of wild lowbush blueberries in a commercially managed wild blueberry field in Maine Northeast USA and used open-top-chambers (OTCs) to study the effects of warming for two years (2019 and 2020). Both active-heating OTCs (elevated monthly mean temperatures by 3.3 °C) and passive-heating OTCs (elevated by 1.2 °C) were employed and compared with ambient controls. Our results showed that warming did not change the prevalence of red leaf disease, blueberry gall midge, red-striped fireworm, or any weed species. In contrast, the incidence of Sphaerulina leaf spot, powdery mildew, and other leaf spot disease were significantly lower under warming treatments compared to the ambient control at the end of the growing season in 2020. Overall, different pests responded to warming differently, inviting further research to reveal the mechanisms. The lower overall pressure of leaf spot disease under warming was probably due to decreased air humidity.

Environmental behaviour of blueberry production at small-scale in Northern Spain and improvement opportunities

The production of berry, a fruit highly appreciated by consumers, has considerably increased in the last years. Europe Union has 1.55 millions of smallholdings dedicated to blueberry farming. Actually, it is an important sector on Spain agrifood exports, especially in Asturias. In order to increase sustainability in crops, it is necessary to identify the key environmental aspects. Blueberry cultivation methods are well documented in bibliography, but Life Cycle Assessment (LCA) researches are virtually inexistent in Europe. For this reason, this work has studied from an environmental perspective four blueberry orchards sit in Asturias (Northern Spain) and the field agricultural practises used. LCA results showed that the main environmental impacts of blueberry production were originated by the use of fertilisers, followed by the use of fossil fuels, plastic and paper materials and incineration of pruning. Significant differences between conventional and organic crops have not been found. The carbon footprints (CF) obtained in this study were between 0.32 kg and 1.66 kg CO2-eq/kg blueberry. CF values obtained in two of the systems analysed were within the literature ranges, between 0.20 and 0.80 kg CO2-eq. per kg of fruit. However, higher values were found in the other two orchards. Environment improvements have been proposed for each particular case. Results here obtained could be used to support policy changes, such as the implementation of sustainable practices in order to lead to a more sustainable blueberry production with lower GHG emissions.

Water, Energy and Nutrient Losses from Food Wastage of Selected Crops in Three Agro-Climatic Zones in British Columbia, Canada

Food waste is a growing global concern. Data on the factors and magnitude are largely global estimates, thus local studies aid in providing information on the impacts of food waste. Three important agro-climatic zones in British Columbia and nine common crops, both annual and perennial, were selected to evaluate the environmental and nutritional implications of local food waste. Using Canadian estimates of total food waste, the constituent water, caloric content, protein, vitamin C, phosphorus and potassium wasted by each crop were estimated. Regionally, the total production and losses were the highest in the Lower Fraser Valley which had high production of potatoes and blueberries, followed by the Okanagan, with grapes and apples, and Vancouver Island, with potatoes. Virtual water was estimated by the BC Agriculture Water Calculator and used to assess the soil and climatic factors impacting the local water demand. Although soil texture seemed to influence water demand, the agro-climatic zone was the main factor controlling the water demand and the corresponding amount of water wasted. Dry agro-climatic zones had annual virtual water up to two times higher for the same crop and soil texture. Lower water demand crops, finer soils and more efficient irrigation systems were more congruent with water stress scenarios. Total losses for each region were based on conservative estimates and would have supplied the caloric energy and protein for over 40,000 adults, and vitamin C for over 300,000 adults for one year. Additionally, the total N, P and K wasted accounted for up to 32, 2 and 13 kg/ha respectively for common fertilizers used in British Columbia. This study confirmed the significance of food waste impacts on local water demand, human nutrition and soil management based on regional data for representative crops.

Leaf anthracnose and defoliation of blueberry caused by Colletotrichum helleniense in Northern Italy

Highbush blueberry is an increasingly important crop due to its economic value and demonstrated health benefits of blueberries. Leaf spots are considered as minor diseases of blueberry plants, but they adversely affect blueberry productivity, causing reduced photosynthetic activity, flower bud formation and berry production. Surveys of blueberry crops were conducted in Piedmont, Northern Italy, during 2019-2020. Fungi isolated from leaf spots of the blueberry cultivar ‘Blue Ribbon’ were identified as Colletotrichum helleniense through a robust multi-locus phylogeny. Eight genomic loci were considered: tub, gapdh, act, cal, his3, chs-1, ApMat and gs. Morphological characters of a representative strain were assessed. Pathogenicity was confirmed on four blueberry cultivars, although with different levels of aggressiveness to the cultivars. This study shows the importance of a polyphasic approach to investigate species of Colletotrichum, and the relevance of molecular tools for the species-level characterization within the ‘Kahawae’ clade. This is the first report of Colletotrichum helleniense causing leaf anthracnose on Vaccinium corymbosum.

Environmental and Fecal Indicator Organisms on Fruit Contact Surfaces and Fruit from Blueberry Mechanical Harvesters

Although previous studies have examined microbial loads on food contact surfaces in blueberry packing plants, there is currently no information regarding microbial risks associated with mechanical berry harvesters used in commercial blueberry production. In this study, we surveyed up to nine fruit contact surfaces on seven mechanical harvesters in each of 2015 and 2016 in the field. These surfaces included the shaking rods at the front of the harvester, the sidewalls of the harvesting tunnel behind the shaking mechanism, the catcher plates collecting the detached berries, horizontal and vertical fruit conveyor belts, and berry lugs collecting the fruit at the back of the harvester. Swab samples were collected from each surface three times a day (morning, noon, and evening) and assessed for environmental and fecal indicator organisms including total aerobes, total yeasts and molds, coliforms and fecal coliforms, and enterococci. At the same time points, fruit samples were assessed for microbial loads before the fruit entered each harvester and after they exited the harvester. Results showed statistically significant differences in microbial loads among harvester surfaces, whereas the effect of sampling time was generally not significant. High levels of total aerobes and total yeasts and molds were recorded, especially on horizontal surfaces and/or those located at the bottom of the harvester such as the lower sidewall, the catcher plates, and the horizontal conveyor belt. These surfaces therefore should be targeted by cleaning and sanitization practices. There was also statistical evidence that passage through the harvester may increase the levels of the environmental microorganisms on fruit in the field. In contrast, fecal indicator organisms such as fecal coliforms and enterococci were detected only sporadically and at very low densities on harvester surfaces and blueberry fruit, and there was no evidence that passage through the harvester increased their levels on the fruit. Berry lugs consistently harbored microbial loads, and given their movement back and forth between the field and the packing plant, deserve particular attention with regard to cleaning, sanitization, and storage protocols.

Fruit Quality and Yield of Three Highbush Blueberry (Vaccinium corymbosum L.) Cultivars Grown in Two Planting Systems under Different Protected Environments

Due to the increasing interest in highbush blueberry (Vaccinium corymbosum L.) among consumers, together with the problems of climate change and specific substrate requirements, a novel approach to intensive blueberry production is required. Here, ‘Duke’, ‘Aurora’, and ‘Brigitta’ blueberry cultivars were planted under the protective environments of a high tunnel and black hail net, each using ridge and pot planting systems. The high tunnel increased the maximal air temperature on average by 7.2 °C compared to the hail net. For all three cultivars, harvest began 6 to 18 days earlier under the high tunnel than under the hail net; however, lower yields and individual phenolics contents were obtained for the fruit. In ‘Aurora’ and ‘Brigitta’, environmental conditions under the high tunnel also reduced plant volume and fruit sugar/organic acid ratio. Growing blueberry plants in 60 L pots had no negative effects on plant volume and fruit ripening time, yield, firmness, color, and chemical composition. This study represents the first to compare highbush blueberry grown under the high tunnel and hail net protective environments using ridge and pot planting systems across three different cultivars. Here, we can conclude that optimal highbush blueberry production of ‘Duke’, ‘Aurora’, and ‘Brigitta’ under the climate conditions of the study provides earlier ripening times under the high tunnel. However, according to fruit yield and quality, all three cultivars benefit from the hail net over the high tunnel, while ‘Duke’ and ‘Brigitta’ also benefit in particular from the hail net combined with growth in pots.

Identificación de clústeres productivos en el cultivo de arándanos en Chile central

The understanding of the productive characteristics and tendencies of fruit producer’s territorial concentration can be explained starting from the singularities of the territory, the production techniques or the market conditions. This article analyzes the formation of clusters of blueberry producers in the Maule region in central Chile, based on the productive characteristics of the crops that include the technological levels, the age of the plantations and the size of the farms. For this, a two-step cluster analysis was performed to obtain homogeneous groups or conglomerates. The results obtained were analyzed in the ArcGIS software, using the Ripley K function, to determine their spatial concentration and to relate the spatial location of the orchards belonging to each cluster and their geographical distribution. The analysis indicates the existence of four clusters in the region, differing preferably by the sizes of the farms. The predominant technological level between the clusters identified is the intermediate, followed by the advanced. The clusters tend to generate spatial and geographic concentrations related with communication facilities and agroecological conditions (climate, soil, relief features). Results founded can improve focus of public efforts and private investments in the productive activity of blueberry cultivation.

Diversity and Pathogenicity of Diaporthe Species Revealed from a Survey of Blueberry Orchards in Portugal

Blueberries (Vaccinium corymbosum) are widely cultivated worldwide and largely consumed due to their known antioxidant and medicinal properties. Although Diaporthe species have been documented in Portugal as causal agents of blueberry twig blight and dieback, there is still scarce information on the species that cause these symptoms. Moreover, Diaporthe vaccinii, recently synonymized with D. eres, has been considered a concern to blueberry production worldwide. However, the current knowledge about its impact on blueberries remains unclear. The diversity of Diaporthe species associated with diseased blueberry plants were assessed through a national survey. A multilocus sequence analysis of the rDNA internal transcribed spacer (ITS) region, the translation elongation factor 1-alpha (tef1-α), β-tubulin (tub2), calmodulin (cal) and histone 3 (his3) genes unveiled the presence of Diaporthe ambigua, D. amygdali, D. crousii, D. foeniculina, D. hybrida, D. leucospermi, D. malorum and D. rudis. Moreover, all species were fully characterized based on a detailed morphological description. Diaporthe amygdali, D. hybrida, D. leucospermi and D. malorum are reported for the first time on diseased blueberries in Portugal. Results show that D. eres exhibited a high level of intraspecific variability within isolates, given that the strain CBS 160.32 might be a minor pathogen on blueberry plants, whereas CAA829 was revealed to be the most aggressive. Overall, this study also demonstrates that Diaporthe amygdali and D. eres may be two of the most aggressive species to blueberry plants. This study improves our understanding of the Diaporthe species and it’s causing of dieback and twig blight on Portuguese blueberry orchards. Additionally, the identification of these pathogens represents crucial information for blueberry producers to apply appropriate phytosanitary measures, as well as offering new insights into the potential pathogenicity of D. eres on this host.