Chicken Blood Research Articles

The Effect of Adding Herbal Extracts to Drinking Water on Body Temperature, Level of Thyroid Hormones and H:L Ratio in the Blood of Broiler Chickens Exposed to Elevated Ambient Temperature

Abstract The aim of the study was to determine the effect of supplementing drinking water with extracts from lemon balm, sage and nettle on body temperature, level of thyroid hormones and the heterophil to lymphocyte (H:L) ratio in the blood of broiler chickens exposed to elevated rearing temperature. One-day-old Ross 308 chicks were divided into four groups: group I (CON) and experimental groups II (LB), II (S) and IV (N), in which, from 22 to 42 days of rearing, drinkers were supplemented with lemon balm extract, sage extract or nettle extract (2 ml/l water), respectively. In addition, at 5 weeks of growth, all the groups were exposed to elevated ambient temperature (up to 30°C) for 5 days, after which the recommended thermal conditions were reinstated. During the study, mortality, rectal temperature and radiated temperature of the broilers were monitored. Blood was collected from 10 birds per group to determine the concentration of thyroid hormones (T3 and T4) and to make blood smears. The H:L ratio was determined based on the percentage of heterophils (H) and lymphocytes (L). The herbal extracts from lemon balm, sage and nettle, added to drinking water at 2 ml/l, reduced the blood level of the thyroid hormone (triiodothyronine) and rectal temperature in the experimental broilers during the initial period of thermal stress. Chickens receiving the nettle extract were also characterised by lower radiated temperature of the unfeathered body, a lower H:L ratio in the blood during the increase in ambient temperature, and the lowest mortality percentage. It can therefore be considered that the dietary supplementation of nettle, in the form and concentration used in this study, had the most favourable effect on the physiological status of the birds (body temperature, level of thyroid hormones and H:L ratio) exposed to elevated ambient temperatures (30°C), and thus on their welfare.

Mechanical Examination of Chicken Vessel with Custom-Built Experimental Equipment

Abstract Cardiovascular disease has been decimating humanity for decades. In vivo examination of blood vessels is of great help in the development of numerical models and simulations that can help physicians significantly improve sufferers’ quality of life. For such models, the different mechanical characteristics of the vessels are the input data. Several such mechanical properties of the vessels, such as modulus of elasticity and tensile strength, are determined by a tensile test. In the course of our research, an experimental device was developed and tested which is suitable for biaxial tensile tests of blood vessels, which we present through the examination of chicken blood vessels.

Extraction RNA Protocol from Blood and Testis Tissue of Local Roosters

The (one-step) method has become widely used to isolate total RNA from living organism samples and from different tissues. The aim of this study is to extract RNA from the blood and testicular tissue of male local chickens. The principle behind the method is to separate the RNA from the DNA after extraction with an acid solution containing (Acid guanidinium thiocyanate-phenol-chloroform) with centrifugation, under acidic conditions, the total RNA remains in the upper aqueous phase while most of the acid descends. The DNA and proteins are either in the interphase or in the lower organic phase and then the total RNA is collected by precipitation with isopropanol. Our results showed a significant increase in the concentration of RNA extracted from the blood compared to the testis tissue of local roosters. This protocol made it possible to isolate RNA from cells and tissues in less than 4 hours and was the reason for the great advances in gene expression analysis in plant and animal models, as well as in pathological samples as clearly demonstrated by the huge number of citations the protocol has gained in the past 20 years.

Interaction of Salmonella Gallinarum and Salmonella Enteritidis with peripheral leucocytes of hens with different laying performance

Salmonella enterica ssp. enterica serovars Enteritidis (SE) and Gallinarum (SG) cause different diseases in chickens. However, both are able to reach the blood stream where heterophils and monocytes are potentially able to phagocytose and kill the pathogens. Using an ex vivo chicken whole blood infection model, we compared the complex interactions of the differentially host-adapted SE and SG with immune cells in blood samples of two White Leghorn chicken lines showing different laying performance (WLA: high producer; R11: low producer). In order to examine the dynamic interaction between peripheral blood leucocytes and the Salmonella serovars, we performed flow cytometric analyses and survival assays measuring (i) leucocyte numbers, (ii) pathogen association with immune cells, (iii) Salmonella viability and (iv) immune gene transcription in infected whole blood over a four-hour co-culture period. Inoculation of blood from the two chicken lines with Salmonella led primarily to an interaction of the bacteria with monocytes, followed by heterophils and thrombocytes. We found higher proportions of monocytes associated with SE than with SG. In blood samples of high producing chickens, a decrease in the numbers of both heterophils and Salmonella was observed. The Salmonella challenge induced transcription of interleukin-8 (IL-8) which was more pronounced in SG- than SE-inoculated blood of R11. In conclusion, the stronger interaction of monocytes with SE than SG and the better survivability of Salmonella in blood of low-producer chickens shows that the host–pathogen interaction and the strength of the immune defence depend on both the Salmonella serovar and the chicken line.

Increased Hatchability of Chickens against the Background of the Use of Water-Soluble Antioxidants

The use of antioxidants is an effective means of increasing egg production and hatchability of chickens. The difficulty in application is in the methods of administration of drugs to chickens. Fat-soluble antioxidants are mainly available on the market.
 Aims: The aim of the study was to study the effectiveness of water-soluble antioxidants on physiological and zootechnical indicators of egg incubation and hatchability of offspring.
 Methodology: The study was conducted on two groups of laying hens of Ostad, selected by random sampling of one hundred heads per group. For 41 days, the chickens of the experimental group received a basic diet enriched with dihydroetoxychine (DHE) in order to increase the antioxidant status at a dosage of 100 mg/kg of feed. Samples were taken from the axillary vein on the 25th day of application of the supplement (n=5) for physiological and biochemical studies.
 Results: During the study, it was found that in the experimental group, the concentration of cholesterol in the high-density lipoprotein fraction doubled (P<0.01), and in the low-density lipoprotein fraction decreased by almost 50% (P<0.01) compared to the control. The concentration of malondialdehyde (MDA) in the blood of chickens of the experimental group was 82.00% relative to that in the control, and in the egg yolk – only 37.42%. Egg production of chickens of the experimental group exceeded the control by 7.27%.
 Conclusion: The totality of the information provided confirms the physiological adequacy for laying hens of the introduction of dihydroethoxychine in the specified dose.

Biological effects of iodine, selenium, sulfur citrates in broiler chickens

This study aimed at the assessment of the I, Se, S citrate effect on the organism of broiler chickens. Feeding of chickens of all groups was carried out by using standard balanced feeds – starter, grower, and finish with the use of group feeders placed at the appropriate height from the floor depending on the poultry height. For the chickens of experimental groups, I, Se, S citrate was added to drinking water daily during the 48th day. To the drinking water of chickens of experimental groups different amounts of the citrate solution were added I (200 mg/L), Se (50 mg/L), S (300 mg/L), prepared by the nanotechnology method. Chickens of the II group received the lowest dose of I, Se, S at the rate of 5 μg I/L, 1.25 μg Se/L, 7.5 μg S/L of drinking water, and for poultry of other experimental groups, it was increased by 2 (III), 4 (IV), 6 (V) and 8 (VI) times compared to II group. Coccidiostat "Kokcisan 12%" (KRKA, Slovenia) was added to the starter and grower feed at the stages of its manufacture in quantities of 0.5 kg/t of feed. Coccidiostat was not added to the final feed. During the 48-day technological period, the clinical condition of the poultry was monitored daily by forage and motor activity, safety, and every 7 days – for growth intensity with the individual determination of body weight on a laboratory scale. The investigation of the blood was carried out from the 35th or 48th day during the growing, period and also investigation of the internal organs – liver, heart, spleen, stomach, thymus, and bursa of Fabricius. The increase of cholesterol, triacylglycerols, and albumin in the blood of II, III, and VI chicken groups was established on the 35th day of the experiment, and the reduction of creatinine in III and VI groups. By that time, the effect of highest dose of I, Se, S caused a decrease in the T3 concentration in the blood of chickens in the VI group compared to the I and II groups. In particular, in the blood of chickens of IV, V and VI groups compared to the I, II, III group under these conditions there was a significant increase in the content of urea, Ca, P, cholesterol, as well as albumin – only in IV group on the 48th day. Triacylglycerols’ content increased in the blood serum of chickens in III and IV groups compared to the II and V groups, but in V group – decreased compared to the III, IV groups. The increase in liver, thymus, and heart mass in the II and V chicken groups was noticed and their decrease in the VI group was revealed compared with the control group. Reliable lower coefficients of the body mass of chickens of III and VI groups may indicate a less notable stimulating biological effect of I, Se, S citrate in these doses on liver development than on the whole organism. The addition of I, Se, S citrate to broiler chickens during the period of growing revealed the difference in their biological action in the presence and absence of the Kokcisan 12% in a mixed fodder. A more notable stimulating effect of low dose of I, Se, S citrate on the growth and development on the chickens' organism was established during the withdrawal of coccidiostatics from their diet. The effect may be due to its inhibitory influence on the biological activity of these elements in the first 35 days of growing. In the final period of chicken growing and withdrawal of ionophore Kokcisan from the diet more noticeable changes were revealed in the studied parameters.

Effect of different doses of silicon dioxide on the concentration of organic acids in the broilers liver

The article presents the results of a study of the amino acid and fatty acid composition of the liver of Arbor Acres broilers, which were fed with ultrafine particles of silicon dioxide at a dose of 100, 200, 300 and 400 mg per kilogram of mixed feed. It was found that the use of UFP SiO2 in the cultivation of broilers contributes to an increase in the liver of poultry I-IV experimental groups of arginine content by 0.45-0.70%, histidine by 0.75-1.60%. The content of leucine, isoleucine, valine, threonine, and phenylalanine increased in the liver of broilers of the III-IV experimental groups by 0.11-0.96%, 0.1-0.6%, 0.10-0.13%, 0.13-0.47%, 0.23-0.53% compared to the control group. The content of linoleic acid in the liver samples of broilers of the I-IV experimental groups increased by 1.80-36.4%, the content of linolenic acid in the liver of experimental chickens of the III-IV experimental groups increased by 0.1-0.2%, and oleic acid – by 0.6-3.3%, respectively. A sharp increase in the content of AST and ALT in the blood of broiler chickens of the experimental groups was not observed, although there is a dose-dependent increase in the activity of these enzymes, which indicates an increase in liver function against the background of the action of ultrafine silicon dioxide.

Influence of new lactulose-containing fodder additives on basic morpho-biochical indicators of blood and resistance of broiler chicken

In modern industrial broiler poultry farming, the issue of combating the uncontrolled use of antibiotics in the process of growing poultry is being actively addressed. Currently, scientists are looking for an alternative solution to the issue of eliminating the use of antibiotics for prophylactic purposes. In this connection, researchers are intensively working on the development of innovative feed additives that can solve the problem with the immunostatus of the organism of poultry grown in conditions of great crowding. The article discloses the data of experimental experience on the use of new innovative lactulose-containing additives of domestic origin "Kumelakt-1" and "Laktuvet-1". Their positive effect on the morpho-biochemical parameters of the blood and the resistance of the poultry organism has been established. This information allows to predict in vivo the indicators of the safety of the poultry population and are indicators of the quality of the products obtained. New lactulose-containing innovative additives as a regulator of intestinal microflora improved the morpho-biochemical blood index and increased the resistance of the poultry organism. Namely: increased on average in I and II experimental groups in comparison with the control group, the hemoglobin index by 9.7%, erythrocytes by 10.8%, hematocrit by 5.6%. An increase in the level of urea in the blood serum was established by 10.4 and 9.7% (P <0.01), glucose - by 6.5 and 4.8 6.1 and 9.0% (P <0.05), phosphorus - by 8.2 and 6.6% (P <0.01), magnesium - by 9.9 and 9.1% (P <0.05), potassium - by 1.3 and 1.6% (P <0.05), iron - by 1.4 and 1.6% (P <0.05), respectively. The indicators of phagocytic activity in the experimental groups were determined, it was found that, on average, this indicator increased by 5.2% (P <0.05).

Differential responses of chicken monocyte-derived dendritic cells infected with Salmonella Gallinarum and Salmonella Typhimurium

Salmonella enterica serovar Gallinarum is a host-restricted bacterial pathogen that causes a serious systemic disease exclusively in birds of all ages. Salmonella enterica serovar Typhimurium is a host-generalist serovar. Dendritic cells (DCs) are key antigen-presenting cells that play an important part in Salmonella host-restriction. We evaluated the differential response of chicken blood monocyte-derived dendritic cells (chMoDCs) exposed to S. Gallinarum or S. Typhimurium. S. Typhimurium was found to be more invasive while S. Gallinarum was more cytotoxic at the early phase of infection and later showed higher resistance against chMoDCs killing. S. Typhimurium promoted relatively higher upregulation of costimulatory and other immune function genes on chMoDCs in comparison to S. Gallinarum during early phase of infection (6 h) as analyzed by real-time PCR. Both Salmonella serovars strongly upregulated the proinflammatory transcripts, however, quantum was relatively narrower with S. Gallinarum. S. Typhimurium-infected chMoDCs promoted relatively higher proliferation of naïve T-cells in comparison to S. Gallinarum as assessed by mixed lymphocyte reaction. Our findings indicated that host restriction of S. Gallinarum to chicken is linked with its profound ability to interfere the DCs function. Present findings provide a valuable roadmap for future work aimed at improved vaccine strategies against this pathogen.

Molecular detection and genetic diversity of Leucocytozoon sabrazesi in chickens in Thailand

Leucocytozoon sabrazesi is the intracellular protozoa of leucocytozoonosis, which is transmitted by the insect vectors and affects chickens in most subtropical and tropical regions of the globe, except South America, and causing enormous economic losses due to decreasing meat yield and egg production. In this study, L. sabrazesi gametocytes have been observed in the blood smears, and molecular methods have been used to analyse the occurrence and genetic diversity of L. sabrazesi in blood samples from 313 chickens raised in northern, western and southern parts of Thailand. The nested polymerase chain reaction (nested PCR) assay based on the cytb gene revealed that 80.51% (252/313) chickens were positive of L. sabrazesi. The phylogenetic analysis indicated that L. sabrazesi cytb gene is conserved in Thailand, showed 2 clades and 2 subclades with similarity ranged from 89.5 to 100%. The diversity analysis showed 13 and 18 haplotypes of the sequences from Thailand and from other countries, respectively. The entropy analyses of nucleic acid sequences showed 26 high entropy peaks with values ranging from 0.24493 to 1.21056, while those of amino acid sequences exhibited 5 high entropy peaks with values ranging from 0.39267 to 0.97012. The results; therefore, indicate a high molecular occurrence of L. sabrazesi in chicken blood samples with the associated factors that is statistically significant (p < 0.05). Hence, our results could be used to improve the immunodiagnostic methods and to find appropriate preventive control strategies or vaccination programs against leucocytozoonosis in order to mitigate or eliminate the harmful impact of this infection on chicken industry.

A Rapid and Simple Assay Correlates In Vitro NetB Activity with Clostridium perfringens Pathogenicity in Chickens.

Necrotic enteritis is an important enteric disease in poultry, caused by NetB-producing Clostridium (C.) perfringens strains. As no straight-forward method to assess the NetB activity of C. perfringens was available, we aimed to develop an easy, high-throughput method to measure the NetB activity produced by C. perfringens. First, the appearance of C. perfringens on different avian blood agar plates was assessed. Based on the size of the haemolysis surrounding the C. perfringens colonies, NetB-positive strains could phenotypically be discriminated from NetB-negative strains on both chicken and duck blood agar. Additionally, strains producing the consensus NetB protein induced more pronounced haemolysis on chicken blood agar as compared to the weak outer haemolysis induced by A168T NetB-variant-producing C. perfringens strains. Next, a 96-well plate-based haemolysis assay to screen NetB activity in the C. perfringens culture supernatants was developed. Using this assay, a positive correlation between the in vitro NetB activity and virulence of the C. perfringens strains was shown. The developed activity assay allows us to screen novel C. perfringens isolates for their in vitro NetB activity, which could give valuable information on their disease-inducing potential, or identify molecules and (bacterial) metabolites that affect NetB expression and activity.

Antioxidant Status of Broiler Chickens Fed Diets Supplemented with Vinification By-Products: A Valorization Approach.

Vinification by-products display great potential for utilization as feed additives rich in antioxidant compounds. Thus, the effect of dietary ground grape pomace (GGP), wine lees extract rich in yeast cell walls (WYC), and grape stem extracts (PE) on the relative expression of several genes involved in liver oxidative mechanisms and the oxidative status of the blood and breast muscle of broiler chickens was investigated. In total, 240 one-day-old as hatched chicks (Ross 308) were assigned to four treatments, with four replicate pens and 15 birds in each pen. Birds were fed either a basal diet (CON) or a basal diet supplemented with 25 g/kg GGP, or 2 g/kg WYC, or 1 g starch including 100 mg pure stem extract/kg (PE) for 42 days. The polyphenolic content of vinification by-products was determined using an LC-MS/MS library indicating as prevailing compounds procyanidin B1 and B2, gallic acid, caftaric acid, (+)-catechin, quercetin, and trans-resveratrol. Body weight and feed consumption were not significantly affected. The relative transcript level of GPX1 and SOD1 tended to increase in the liver of WYC-fed broilers, while NOX2 tended to decrease in the PE group. SOD activity in blood plasma was significantly increased in WYC and PE compared to the CON group. The total antioxidant capacity measured with FRAP assay showed significantly higher values in the breast muscle of PE-fed broilers, while the malondialdehyde concentration was significantly decreased in both WYC- and PE-fed broilers compared to the CON group. The exploitation of vinification by-products as feed additives appears to be a promising strategy to improve waste valorization and supply animals with bioactive molecules capable of improving animals’ oxidative status and products’ oxidative stability.

Surveillance of West Nile virus infection in Kashgar Region, Xinjiang, China, 2013\u20132016

West Nile virus (WNV) was first isolated in mainland China from mosquitoes in Jiashi County, Kashgar Region, Xinjiang in 2011, following local outbreaks of viral meningitis and encephalitis caused by WNV. To elaborate the epidemiological characteristics of the WNV, surveillance of WNV infection in Kashgar Region, Xinjiang from 2013 to 2016 were carried out. Blood and CSF samples from surveillance human cases, blood of domestic chicken, cattle, sheep and mosquitoes in Kashgar Region were collected and detected. There were human 65 WNV Immunoglobulin M (IgM) antibody positive cases by ELISA screening, 6 confirmed WNV cases by the plaque reduction neutralization test (PRNT) screening. These cases occurred mainly concentrated in August to September of each year, and most of them were males. WNV-neutralizing antibodies were detected in both chickens and sheep, and the positive rates of neutralizing antibodies were 15.5% and 1.78%, respectively. A total of 15,637 mosquitoes were collected in 2013–2016, with Culex pipiens as the dominant mosquito species. Four and 1 WNV-positive mosquito pools were detected by RT-qPCR in 2013 and 2016 respectively. From these data, we can confirm that Jiashi County may be a natural epidemic foci of WNV disease, the trend highlights the routine virology surveillance in WNV surveillance cases, mosquitoes and avian should be maintained and enhanced to provide to prediction and early warning of outbreak an epidemic of WNV in China.

Lead Levels in Tissues of Local Scavenger Chickens in Maiduguri, Nigeria

Mineral mining activities with environmental pollution in Nigeria resulted in lead intoxication in humans and livestock. The likelihood of lead contamination of the environment from non-mining sources may exist in Maiduguri, Borno state, Nigeria. The study was carried out to evaluate the bioaccumulation of lead in extensively reared local scavenger chickens-LSC (Gallus gallusdomesticus) in Maiduguri. Two hundred samples each of the liver, kidney, intestine, muscle and blood were tested for lead using standard methods in toxicologic evaluation. There were significant (p&lt;0.05) variations in lead levels in the kidney, liver, muscle, intestine and blood of local scavenger chickens. The highest mean lead level (17.0±12.0µg/kg) was observed in the liver, and the lowest leadlevel (4.0±7.0µg/kg) was in the blood. No significant (p&gt;0.05) sex differences existed in the frequency of lead detection. Mean lead level in the liver of males was significantly (p&lt;0.05) higher than that of females with the highest and lowest mean lead levels occurring in the liver and kidney of male chickens, respectively. The highest lead level detected was 414 µg/kg; in the kidney of a female LSC. It was therefore concluded that environmental pollution with lead may be detected in LSCthat bioaccumulate lead; with the liver having the highest affinity.

Beneficial of Bioactive Compound of Rice Bran for Chicken’s Functional Feed

&lt;p&gt;Rice bran is a feed ingredient as a result of the rice milling process which widely used as an energy source in the feed formulation in the chicken diet. Rice bran contains various nutrients such as oil, protein, carbohydrates (especially starch), beta-glucans, and pectins. Rice bran contains bioactive compounds that have positive effects on the chicken performance and health. Recently, rice bran’s bioactive compounds have been developed as a source of making functional feed. The objective of this study was to review the bioactive compounds in rice bran and their benefits in order to produce chicken’s functional feed. The results of the study showed that rice bran contains oryzanol as an important bioactive compound that has high antioxidant activity. Furthermore, other bioactive compound, lysolecithin, has an ability to reduce cholesterol content in blood, meat and eggs of chicken. Besides, rice bran extract is reported to exert antibacterial activity and has immunomodulator function. It was concluded that the bioactive compounds of rice bran is potential to be used as a substance for making functional feed for chickens.&lt;/p&gt;

Effect of Inorganic Zinc on Selected Immune Parameters in Chicken Blood and Jejunum after A. galli Infection

Ascaridiosis in poultry results in a reduction in body weight gain, egg production, as well as microelement levels. Infected poultry have higher demands on feed with the addition of essential elements including zinc. The effects of the infection by Ascaridia galli and the supplementation of inorganic zinc on the immune status of broilers were monitored through evaluation of the relative expression of selected genes (interleukins, IFN-γ, and TNF-α) by real-time PCR, haematology parameters by microscopy, and quantitative changes of lamina propria lymphocytes by flow cytometry in day 7 and day 14 of the study. We observed that the enrichment of the diet with inorganic zinc has a positive effect on the relative percentage of CD4+ lamina propria lymphocytes in the jejunum and on heterophil counts in blood. In addition, it was concluded that inorganic zinc has an anti-inflammatory effect (downregulation of TNF-α and IL-17) and activates IgA-producing cells in the jejunum of chicks infected with A. galli.

The influence of biologically active additives on the morpho-biochemical parameters of the blood of broiler chickens

Abstract. The introduction of synbiotics and phytobiotics into the diet allows us to optimize metabolism, increase the level of use of feed components, and activate immune processes in the body. The purpose of the work is to determine the effect of “GerbaStor” and “ProStor” feed additives, including probiotic, prebiotic and phytobiotic components, on the morphological and biochemical parameters of the blood of broiler chickens of the “Ross-308” cross. Methods. In the course of a scientific and economic experiment at the age of 29 days, blood was taken from 5 medium-sized broilers by decapitation to determine morphological and biochemical parameters. The blood test was carried out at the Clinical and Diagnostic Center in Ekaterinburg. The morphological examination of blood was carried out in the hemostasis laboratory, on an automatic analyzer ADVIA 120, manufactured by BAYER, as well as by manual counting of formed elements in the Goryaev chamber. A biochemical blood test was performed in a clinical diagnostic laboratory using a Vitros 350 analyzer. Results. The inclusion of “GerbaStor” and “ProStor” in the diet did not adversely affect the metabolic processes of the bird. Morpho-biochemical blood parameters were within physiological values. In the experimental groups was observed an improvement in the assimilation of protein nitrogen, it is evidenced by a decrease in uric acid in the blood serum. In addition, the use of the studied feed was accompanied by the activation of lipid metabolism in the body of birds, which was characterized by a tendency to reduce the amount of triglycerides in the blood serum in individuals of the 1st and 2nd experimental groups. The results of the study showed that when “ProStor” is introduced into the feed concentrate, there is a tendency of decreasing in the activity of transaminases in the body of broilers, which demonstrates a higher resistance of their internal organs, in particular the liver and heart, to unfavorable exogenous and endogenous influences. The academic novelty lies in the fact that new data were obtained on the influence of the studied feed factors, including probiotic, prebiotic and phytobiotic components, on the morpho-biochemical parameters of the blood of broiler chickens.

Effects of Vaccination Against Coccidiosis on Gut Microbiota and Immunity in Broiler Fed Bacitracin and Berry Pomace.

Feeding practices have been found to influence gut microbiota which play a major role in immunity of poultry. In the present study, changes in cecal microbiota and humoral responses resulting in the 55 ppm bacitracin (BACI), 1% each of cranberry (CP1) and wild blueberry (BP1) pomace alone or in combination (CP+BP) feeding in broiler Cobb 500 vaccinated or not against coccidiosis were investigated. In the non-vaccinated group, no significant treatment effects were observed on performance parameters. Vaccination significantly affected bird’s performance parameters particularly during the growing phase from 10 to 20 days of age. In general, the prevalence of coccidiosis and necrotic enteritis (NE) was reduced by vaccination (P < 0.05). BACI-treated birds showed low intestinal lesion scores, and both CP1 and BP1 feed supplementations reduced Eimeria acervulina and Clostridium perfringens incidences similar to BACI. Vaccination induced change in serum enzymes, minerals, and lipid levels in 21-day old birds while, levels of triglyceride (TRIG) and non-esterified fatty acids (NEFA) were higher (P < 0.05) in CP1 treated non–vaccinated group than in the control. The levels of NEFA were lower in BACI- and CP1-fed birds than in the control in non-vaccinated day 28 old birds. The highest levels of all estimated three immunoglobulins (IgY, IgM, and IgA) were found in the vaccinated birds. Metagenomics analysis of the cecal bacterial community in 21-day old birds showed the presence of Firmicutes (90%), Proteobacteria (5%), Actinobacteria (2%), and Bacteroidetes (2%). In the vaccinated group, an effect of BACI was noted on Proteobacteria (P = 0.03). Vaccination and/or dietary treatments influenced the population of Lactobacillaceae, Enterobacteriaceae, Clostridiaceae, and Streptococcaceae which were among the most abundant families. Overall, this study revealed that besides their beneficial effects on performance, alike bacitracin, berry pomaces in poultry feed have profound impacts on the chicken cecal microbiota and blood metabolites that could be influenced by vaccination against coccidiosis.

The effect of Noni (Morinda citrifolia) leaf shoot meal as an antioxidant and antibacterial against Salmonella sp. and Escherichia coli

Indonesia as a tropical country is rich in biological resources, among which are known as herbal plants. One of the herbs that are often used is Noni (Morinda citrifolia), which has been reported to possess antioxidant and antimicrobial activities. The objective of this study was to evaluate the antioxidant and antibacterial effects of Noni Leaf Shoot Meal (NLSM) to inhibit Salmonella sp. and Escherichia coli as poultry ration. Two hundred and forty kampong chickens of 4 weeks old were reared until 12 weeks old. The diet treatments were R0= 0% NLSM (control), R1= 0.5% NLSM, R2= 1% NLSM, R3= 1.5% NLSM, R4= 2% NLSM, and R5= 2.5% NLSM. Six treatments and four replications are used with ten kampung chickens per replicate. Data were analyzed using descriptive statistics. The parameters measured were phytochemical analysis, vitamin A, beta carotene, Malondialdehida (MDA) blood, inhibition zone diameter, and Clearance test. Results showed that NLSM could reduce MDA blood and bacterial concentration. It is concluded that lower MDA concentration in kampong chicken blood indicates that NLSM contains high antioxidants. NLSM can reduced bacterial concentration in chicken blood so it can be used as a natural antibacterial to improve kampong chickens production and immune system.

The effectiveness of the use of feed hydrolyzate in the poultry diet

The authors of this article evaluated the effectiveness of the use of a feed additive based on soy protein hydrolysate in combination with iodine and selenium on laying hens of the Ross Hysex Brown cross. During the experiment, the following indicators were studied: safety, live weight gain, egg production, morpho-biochemical composition of blood. At the end of the experiment, the following data were obtained: the survival rate of the bird in the experimental group was 93,3 %, and in the control group the result was 86,6 %. The weight gain of laying hens in the experimental group increased by 11 %, the egg weight (average value) by 6,5 g (12,8 %), and in the control the increase was 2,7 g (5,2 %). In the experimental group, eggs per hen were obtained by 1,87% more than in the control group, in general, the experimental group showed an increase in the number of eggs during the experiment by 23,0 %. According to the results of the morphological and biochemical composition of the blood serum of birds, there are no significant negative changes. Red blood cells did not exceed intraspecific norms, but increased in the experimental group by 26,4 %. The amount of hemoglobin in the experimental group exceeded the control by 19,4 %. White blood cells in the experimental group were 16,9 % higher than this indicator in the control group. Biochemical analysis revealed a positive trend, which was expressed in an increase in the concentration of protein in the blood of chickens of the experimental group by 8,7 %, the level of calcium by 10,9 % and phosphorus by 31,3 % in birds of the experimental group exceeds the control.