Biogenic Metal Research Articles

The Role of Complexes of Biogenic Metals in Living Organisms

Biogenic metals and their various inorganic, organometallic, and coordination compounds are comprehensively studied and extensively used in medical practice. Since the biogenic metals have various chemical properties corresponding to their position in the periodic table, their biological functions are different. Almost all of the discussed biogenic elements have an ability to form coordination complexes. Furthermore, the different accessible oxidation states occupied by most of these elements enables the body to catalyze oxy-reduction interactions, depending on the biological conditions. As they are biogenic in nature, their deficiency or their excess in the body leads to numerous pathological obstructions. The application of metal-based compounds as medications is connected with the oxy-reduction properties and the capability to form coordination complexes, which are involved in many bioreactions. The usefulness of these metals as therapeutic and diagnostic agents is also pointed out.

Interactions of bioactive trace metals in shipboard Southern Ocean incubation experiments

AbstractIn the Southern Ocean, it is well‐known that iron (Fe) limits phytoplankton growth. Yet, other trace metals can also affect phytoplankton physiology. This study investigated feedbacks between phytoplankton growth and dissolved Fe, manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), and cadmium (Cd) concentrations in Southern Ocean shipboard incubations. Three experiments were conducted in September–October 2016 near the West Antarctic Peninsula: Incubations 1 and 3 offshore in the Antarctic Circumpolar Current, and Incubation 2 inshore in Bransfield Strait. Additions of Fe and/or vitamin B12 to inshore and offshore waters were employed and allowed assessment of metal (M) uptake relative to soluble reactive phosphorus (P) across a wide range of initial conditions. Offshore, treatments of >1 nmol L−1 added Fe were Fe‐replete, whereas inshore waters were already Fe‐replete. Results suggest Mn was a secondary limiting nutrient inshore and offshore. No Fe‐vitamin B12 colimitation was observed. Overall, M:P uptake in the incubations was closely related to initial dissolved M:P for Fe, Mn, Co, Ni, and Cd, and for Cu inshore. Final concentrations of Fe and Zn were similar across light treatments of the experiments despite very different phytoplankton responses, and we observed evidence for Co/Cd/Zn substitution and for recycling of biogenic metals as inventories plateaued. In dark bottles, the absence of Mn oxidation may have allowed more efficient recycling of Fe and other trace metals. Our results provide insight into factors governing trace metal uptake, with implications for phytoplankton community composition locally and preformed micronutrient bioavailability in Southern Ocean water masses.

Biological role and food sources of biogenic metals

Biological role and food sources of biogenic metals

FEATURES OF CARBOHYDRATE-LIPID METABOLISM IN DOGS WITH DIFFERENT TYPES OF HIGHER NERVOUS ACTIVITY

The relevance of the study is due to the lack of data on the cortical mechanisms of regulation of carbohydrate and lipid metabolism in the body of dogs. In this regard, this article is aimed at revealing the issue of lipid and carbohydrate metabolism in dogs with various types of higher nervous activity under the influence of short-term food deprivation. It is shown that the effect of short-term food deprivation is characterized by changes in metabolism in the body of dogs, which are limited by the state of the nervous system of these animals. In the intact state, the glucose content in the blood of dogs with different types of higher nervous activity does not reliably differ, while the lactate content in the blood of dogs with a weak type of higher nervous activity is higher by 16.2% (P < 0.01), and pyruvate is lower by 6.3% (P < 0.001) from the indicators of dogs with a strong balanced mobile type. Within a day after the beginning of food deprivation, the glucose content in the blood of dogs decreases by 4.6–8.2% depending on the type of nervous activity (P < 0.05–0.01). During the day after the beginning of deprivation, the ratio of lactate to pyruvate in the blood of dogs, depending on the type of higher nervous activity, increases by 19.1–36.0% (Р < 0.01), the content of total cholesterol and triacylglycerols in the blood plasma decreases by 3, 5–12.9% and the ratio of lipids of different densities changes significantly. In particular, in the blood plasma of dogs of a strong balanced mobile type of higher nervous activity, the cholesterol content of high-density lipoproteins decreased during the day by 7.1% (Р < 0.05). Thanks to the conducted research, it was possible to obtain fundamental knowledge of the cortical mechanisms of regulation of carbohydrates and lipids in the body of dogs, which will form the basis of the development of new, modern methods of correction of metabolism, taking into account the type of higher nervous activity. Prospects for further research consist in the development of new methods of correction of metabolism based on the use of nanoaquachelates of biogenic metals, taking into account the individual characteristics of the body of dogs.

Adsorption isotherms and kinetics for Pb(ii) ion removal from aqueous solutions with biogenic metal oxide nanoparticles.

This study investigates the sorption removal of lead(ii) ions using zinc oxide (ZnO) and copper(ii) oxide (CuO) nanoparticles synthesized through a wet burning method with the aid of plant extract from Serratula coronata L. The effect of plant collection time on polyphenol content was investigated and optimal conditions were determined. The structural and chemical properties of the nanoparticles were studied by scanning electron microscopy, energy dispersive analysis, X-ray phase analysis, and X-ray photoelectron spectroscopy. A comparative analysis of lead ion sorption on the surface of synthesized nanoparticles was conducted. The kinetic study revealed that the sorption process follows a pseudo-second-order mechanism, and the Freundlich sorption model provides a better fit for the experimental data. ZnO and CuO nanoparticles exhibited significant sorption capacities, with values of 163.6 and 153.8 mg g-1, respectively.

Polygalacturonates of Biogenic Metals - Prospective Components of New Composite Preparations for Wheat

New bi- and polymetallic complexes of polygalacturonic acid with essential trace elements for pre-sowing treatment of winter wheat seeds were created.
 Aims: The goal of the research was to create effective environmentally safe preparations of multifunctional action, with low cost rates for grain crops based on natural polymers of pectin nature and to determine the effectiveness of their use for pre-sowing treatment of winter wheat seeds.
 Methodology: Methods of chemical synthesis of products, research of their structure by atomic force microscopy were used in the work. Laboratory and vegetative experiments were carried out. The substrate is quartz sand. Physiological and biochemical methods were used to determine the working absorbing surface of the roots and its absorbing activity, amylolytic activity, the content of photosynthesis pigments according to Wellburn. The results were processed using the methods of mathematical statistics.
 Results: The atomic force microscopy method made it possible to detect a mixture of individual polymer molecules and their aggregates in pectin fractions. Depending on the concentration of pectins in the solution, they form different structures. New bi- and polymetallic complexes of polygalacturonic acid with essential trace elements for pre-sowing treatment of winter wheat seeds were created. According to the results of laboratory and vegetation experiments, the growth-stimulating activity of polygalacturonates of copper, iron, calcium, and nickel, which were used for pre-sowing processing of Smuhlyanka variety winter soft wheat seeds was determined. Preparations contribute to the active development of the root system of plants, strengthening their physiological activity. The investigated compounds are effective promising components of complex compositional preparations for modern agrotechnologies of winter wheat cultivation. 
 Conclusion: The use of such metal complexes at an insignificant rate of expenditure should contribute to the increase of economic and ecological indicators of crop production — the reduction of the cost price of wheat grain due to the increase in yield and grain quality.

Bio-synthesized multi-metal oxides with varying Fe/Mn ratios and transitional metals (Ni, Ce, Al, Cu) for catalytic ozonation

Fe and Mn are frequently used to synthesize ozonation catalysts via physicochemical routes. In this study, we used a manganese oxidizing bacterium Pseudomonas sp. to produce biogenic Fe-Mn oxides (Bio-FeMnOx) and compared the effect of doping a third transitional metal (Ni, Ce, Al, Cu). It was found that Fe/Mn ratios can effectively regulate the catalytic activity of Bio-FeMnOx and Cu showed the best doping effect among the four metals. The degradation kinetic constant of the optimal Bio-FeMnOx-Cu was approx. 3 times that of its chemogenic peer. Electron paramagnetic resonance and probe tests revealed that the reactive oxygen species were •OH, •O2－ and 1O2. The generation of •OH was preferred at pH 2 over pH 7 while •O2－ was generated in the opposite preference. We also observed the multi-valence of all metals in Bio-FeMnOx-Cu whose good stability were confirmed with 5 cycles of usage. The catalytic performance of Bio-FeMnOx-Cu was also confirmed with real wastewater. This study demonstrated the importance of Fe/Mn ratios and doping a third transitional metal regarding the activity of catalyzing ozonation and thus would provide a valuable reference for producing biogenic metal oxides of high catalytic activity.

Phytogenically bioengineered metal nanoarchitecture for degradation of refractory dye water pollutants: A pragmatic minireview

The present scenario of water insecurity (poor availability and accessibility to clean and healthy water when needed) denotes the preponderance of environmental damage caused by effluents or waste runoff from industries, such as the dye and textile industries. The polluted waters have led to terrible and sonorous negative impacts on the ecosystem and econetworking, thus, urging for a sustainably suitable substitute to tackle and annul the noxious environmental constraint posse by the hazardous dye effluent. Several chemical and physical methods for treating dye effluent are now in use, but they are time‐consuming, expensive, and inefficient. Interestingly, nanoparticles have egressed as a superior answer for efficient dye removal and degradation, because of their chemical reactivity and exceptional surface characteristics. As a result, the use of metal nanomaterials in the treatment of dye runoffs has been thoroughly investigated. The study used major scientific databases such as SciFinder, Scopus, PubMed, Google Scholar, and Science Direct to conduct a comprehensive review of publicly available literature. Degradation, dye effluent, green metal nanoparticles, and water pollution were the keywords used to find scholarly papers. Efforts were made to figure out and explain the mechanism of dye effluent degradation, how long the degradation will take and how effective the use of plant biosynthesized metal nanoparticles for dye removal is. In addition, the role of bimetallic nanoparticles has also been investigated with remarkable feat from literature. The current level of knowledge about the mechanism and the use of plant biogenic metal nanoparticles in common dye effluent treatment is summarized in this paper.

To decipher the phytochemical agent and mechanism for Urginea indica mediated green synthesis of Ag nanoparticles and investigation of its antibacterial activity against Methicillin-resistant Staphylococcus aureus

Globally, Methicillin-Resistant Staphylococcus aureus bacteraemia is one of the commonest bloodstream infections associated with clinical complications and high mortality. Thence, devising effective and targeted biogenic silver based strategies are in great demand. However, limited insights regarding the biosynthesis methodologies impedes the possible scale up and commercial potentials. We, hereby demonstrate the biosynthesis of Ag nanoparticles using the phytochemical agent extracted and purified from bulb extract of Urginea indica. The chemical structure of the phytochemical agent is investigated by various chromatographic and spectroscopic techniques and was found closely relatable to N-ethylacetamide. Ag nanoparticles synthesis by this agent was found to have a strong Surface Plasmon band at 402 nm. X-ray diffraction and transmission electron microscopy further validated the formation of Ag nanoparticles with face-centred cubic structure with a size range of 20–30 nm. The biogenic metal nanoparticles have shown potential antibacterial activity against S. aureus and MRSA (within a range of 10–50 μg/mL). The nanoparticles have also shown promising anti-biofim activity against the above mentioned strains. The nanoparticles were expected to induce ROS mediated bactericidal mechamism. Cell viability and in-vitro infection studies advocate noticeable biocompatibility and future clinical potential of the developed nanoparticles against Staphylococcus infections.

Synthesis and Biological Evaluation of PEGylated MWO4 Nanoparticles as Sonodynamic AID Inhibitors in Treating Diffuse Large B-Cell Lymphoma.

Sonodynamic therapy (SDT) triggered by ultrasound (US) has attracted increasing attention owing to its ability to overcome critical limitations, including low tissue-penetration depth and phototoxicity in photodynamic therapy (PDT). Biogenic metal oxide nanoparticles (NPs) have been used as anti-cancer drugs due to their biocompatibility properties with most biological systems. Here, sonosensitizer MWO4-PEG NPs (M = Fe Mn Co Ni) were synthesized as inhibitors to activation-induced cytidine deaminase (AID), thus neutralizing the extensive carcinogenesis of AID in diffuse large B-cell lymphoma (DLBCL). The physiological properties of these nanomaterials were examined using transmission electron microscopy (TEM). The inhibition of NPs to AID was primarily identified by the affinity interaction prediction between reactive oxygen species (ROS) and AID through molecular dynamics and molecular docking technology. The cell apoptosis and ROS generation in US-triggered NPs treated DLBCL cells (with high levels of AID) were also detected to indicate the sonosensitivity and toxicity of MWO4-PEG NPs to DLBCL cells. The anti-lymphoma studies using DLBCL and AID-deficient DLBCL cell lines indicated a concentration-dependent profile. The synthesized MWO4-PEG NPs in this study manifested good sonodynamic inhibitory effects to AID and well treatment for AID-positive hematopoietic cancers.

Adsorption of cytosine on prebiotic siliceous clay surface induced with metal dications: Relevance to origin of life

Adsorption of protobiomolecules on primigenial mineral surfaces transacts a very specific and selective role in prebiotic chemical evolution. Cytosine (CYN) is a core constituent of genetic macromolecules i.e., DNA and RNA. A prebiotic simulation experiment following adsorption behaviour of a nucleic acid base CYN, as a function of pH, concentration, time and temperature on the surface of Montmorillonite (MMT), a prominent siliceous clay of smectite group having specific surface area, was studied systematically and methodically using UV, FTIR, SEM and XRD. Biologically significant metal dication [M2+ = Mg2+, Ca2+, Fe2+ and Cu2+] embeded surfaces of MMT (MMT-M2+) were prepared separately by cation-exchange approach. Results observed through UV spectral data reveal a very significant role of metal ions on the quantity of CYN adsorbed on different solid surfaces. The adsorption behaviour fitted well with Langmuir isotherm model and the adsorption isotherm shows monolayer formation of CYN (as adsorbate) on the surface of MMT and MMT-M2+(as adsorbent) showing Langmuir type adsorption. The calculated Langmuir adsorption parameters (KL& Xm) delineate appreciable interaction of CYN on MMT-Fe2+ surface following MMT-Cu2+ as compared to MMT alone and to other metal induced MMTs.In terms of % binding and Xm data, the effectiveness of various adsorbents in adsorption of CYN on MMT/MMT-M2+ at pH 6.6 and temperature 298K was found in the order; MMT-Fe2+> MMT-Cu2+> MMT > MMT-Ca2+> MMT-Mg2+. The results were further confirmed by SEM, XRD and FTIR which also support the outcomes. The present work throws light on the affinity of MMT clay (having multifarious applications in biological systems) with and without biogenic metal diions towards the surface interaction of nucleic acid base mimicking primeval sea shore/sea bed or hydrothermal vent conditions deciphering the phenomena that how biomonomers could have been protected from extreme hazardous conditions of primitive Earth and stabilized during chemical evolution and availed them for consecutive molecular evolution. Results further embark better adsorption capability of transition metal ion incorporated MMTs than the clay without metal ion and exchanged with alkaline Earth dications.

Biogenic Synthesis of CuO, ZnO, and CuO-ZnO Nanoparticles Using Leaf Extracts of Dovyalis caffra and Their Biological Properties.

Biogenic metal oxide nanoparticles (NPs) have emerged as a useful tool in biology due to their biocompatibility properties with most biological systems. In this study, we report the synthesis of copper oxide (CuO), zinc oxide (ZnO) nanoparticles (NPs), and their nanocomposite (CuO–ZnO) prepared using the phytochemical extracts from the leaves of Dovyalis caffra (kei apple). The physicochemical properties of these nanomaterials were established using some characterization techniques including X-ray diffraction analysis (XRD), ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). The XRD result confirmed the presence of a monoclinic CuO (Tenorite), and a hexagonal ZnO (Zincite) nanoparticles phase, which were both confirmed in the CuO–ZnO composite. The electron microscopy of the CuO–ZnO, CuO, and ZnO NPs showed a mixture of nano-scale sizes and spherical/short-rod morphologies, with some agglomeration. In the constituent’s analysis (EDX), no unwanted peak was found, which showed the absence of impurities. Antioxidant properties of the nanoparticles was studied, which confirmed that CuO–ZnO nanocomposite exhibited better scavenging potential than the individual metal oxide nanoparticles (CuO, and ZnO), and ascorbic acid with respect to their minimum inhibitory concentration (IC50) values. Similarly, the in vitro anticancer studies using MCF7 breast cancer cell lines indicated a concentration-dependent profile with the CuO–ZnO nanocomposite having the best activity over the respective metal oxides, but slightly lower than the standard 5-Fluorouracil drug.

Biogenic Metal and Metal Oxides Nanoparticles as Anticancer Agent: A Review

Herein this review we have summarized a number of cytotoxic studies which have been done using various biosynthesized metal nanoparticles (MNPs) and metal oxide nanoparticles (MONPs) on different cancer cell lines. Plants can serve as a good candidate to provide natural surfactants for the green approach in the preparation of nanoparticles. Numerous bio-molecules are present in the plants. Also, numerous plant extract-based MNPs and MONPs have been synthesized and used in several fields of applications particularly in biomedicine. This property can be attributed because of their low cost, biocompatibility and favourable to the environment. In the past few years, the utilisation of these biogenic nanoparticles has in-creased tremendously particularly in cancer therapy. These biogenic nanoparticles considered as an excellent tool for cancer diagnosis and drug delivery at the tumour site preferentially. By utilising the unique properties of nanoparticles and antioxidant and antitumor nature of plants, these biosynthesised nanoparticles selectively destroy the tumour cells and do not harm the normal healthy cells. In this review, we have compiled the most significant results obtained by the biosynthesized MNPs and MONPs like silver (Ag), gold (Au), Fe2O3/Fe3O4, ZnO, and CuO respectively.

Bio-synthesis of Co-doped FeMnOx and its efficient activation of peroxymonosulfate for the degradation of moxifloxacin

Metal oxides can effectively activate peroxymonosulfate (PMS) to degrade organic contaminants but are usually synthesized via chemical/physical processes involving extreme conditions and hazardous materials. In this study, an innovative bio-synthesis method with a strain of manganese oxidizing bacteria Pseudomonas sp. was developed to prepare multiple metal oxides consisting of iron, manganese, and cobalt (Bio-FeMnCoOx) for degrading moxifloxacin (MOX) with PMS. It was found that the cultivation time, the dosage of Co, the polishing method are the key parameters regulating the PMS activation performance of Bio-FeMnCoOx. Under the optimal preparing conditions, the MOX degradation kinetic constant of obtained Bio-FeMnCoOx was 7 times that of the chemically synthesized peer. Analysis with XRD, EPS and SEM with EDS mapping showed an amorphous structure of Bio-FeMnCoOx with well distributed Fe, Mn, and Co. Radical quenching and EPR spin-trapping tests demonstrated that SO4•- and 1O2 were the main reactive oxygen species. The transformation products of MOX were identified by UPLC-QTOF-MS/MS and the possible degradation pathways were accordingly proposed. The respiratory test showed that the toxicity of MOX was reduced by approximately 69% after the degradation with PMS activated by Bio-FeMnCoOx. The study demonstrates the potential of biogenic metal oxides for efficiently activating PMS.

The Potential Application of Green-Synthesized Metal Nanoparticles in Dentistry: A Comprehensive Review.

Orodental problems have long been managed using herbal medicine. The development of nanoparticle formulations with herbal medicine has now become a breakthrough in dentistry because the synthesis of biogenic metal nanoparticles (MNPs) using plant extracts can address the drawbacks of herbal treatments. Green production of MNPs such as Ag, Au, and Fe nanoparticles enhanced by plant extracts has been proven to be beneficial in managing numerous orodental disorders, even outperforming traditional materials. Nanostructures are utilized in dental advances and diagnostics. Oral disease prevention medicines, prostheses, and tooth implantation all employ nanoparticles. Nanomaterials can also deliver oral fluid or pharmaceuticals, treating oral cancers and providing a high level of oral healthcare. These are also found in toothpaste, mouthwash, and other dental care products. However, there is a lack of understanding about the safety of nanomaterials, necessitating additional study. Many problems, including medication resistance, might be addressed using nanoparticles produced by green synthesis. This study reviews the green synthesis of MNPs applied in dentistry in recent studies (2010–2021).

Study of the efficiency of using biogenic metals for feeding calves

Breeding young cattle is important, especially during the transition period from dairy feeding to concentrated dry feed. The paper presents the results of the use of biogenic metal nicotinates for weaning calves to improve metabolism in animals.
 The aim of research. To investigate the effect of biogenic metal nicotinates: Zn, Cu, Fe, Co, Mn on the biochemical parameters of blood in calves after weaning.
 Materials and methods. The study was carried out during 2021 in the conditions of LLC "Agrofirma Lan", Sumy region, Sumy district, Kindrativka, Ukraine for breeding young cattle. The calves of the experimental group were given compound feed and a premix of nicotinates of biogenic metals: Zn, Cu, Fe, Co, Mn, manufactured by PPronos Agro" (1 g per 1 kg of feed). In the control group, combined feed and a premix with metal sulfates were used for 30 days.
 Results. An increase in the level of total protein in the body of calves of the experimental groups was established by 16.12 % in comparison with the control (p≤0.05). Also, in experimental animals, the activity of the enzymes alanine aminotransferase and aspartate aminotransferase was higher than the physiological norm, which shows an insignificant effect of nicotinates of biogenic metals on internal organs and systems. In the experimental group of calves, the level of magnesium was probably higher by 52.38 % and potassium – by 14.94 % compared to the control group (p≤0.05). It was found that the animals of the experimental groups probably had more zinc by 34.96 %; copper – by 35.72 %; iron – by 92.29 %; manganese – by 41.13 %; selenium – by 3.22 % and cobalt – by 98.33 % compared to the control (p≤0.05).
 Conclusions. The positive effect of the use of biogenic metal nicotinates on the metabolism of calves at weaning has been proven. It was found that the level of total protein in the body of calves of the experimental groups was probably higher by 16.12 %, magnesium - by 52.38 %; potassium - by 14.94 %. When determining the content of inorganic substances, it was found that the animals of the experimental groups probably had more zinc by 34.96 %; copper – by 35.72 %; iron – by 92.29 %; manganese – by 41.13 %; selenium – by 3.22 % and cobalt – by 98.33 % compared to the control (p≤0.05)

Diversity of Microbial Metal Sulfide Biomineralization.

Since the emergence of life on Earth, microorganisms have contributed to biogeochemical cycles. Sulfate-reducing bacteria are an example of widespread microorganisms that participate in the metal and sulfur cycles by biomineralization of biogenic metal sulfides. In this work, we review the microbial biomineralization of metal sulfide particles and summarize distinctive features from exemplary cases. We highlight that metal sulfide biomineralization is highly metal- and organism-specific. The properties of metal sulfide biominerals depend on the degree of cellular control and on environmental factors, such as pH, temperature, and concentration of metals. Moreover, biogenic macromolecules, including peptides and proteins, help cells control their extracellular and intracellular environments that regulate biomineralization. Accordingly, metal sulfide biominerals exhibit unique features when compared to abiotic minerals or biominerals produced by dead cell debris.

Coordination compounds of biogenic metals as cytotoxic agents in cancer therapy

The review summarizes the data on the structures and methods for the synthesis of compounds with anticancer activity based on biogenic metals, which can replace platinum drugs prevailing in cytotoxic therapy. The main focus is given to the comparison of the mechanisms of the cytotoxic action of these complexes, their efficacy and prospects of their use in clinical practice. This is the first systematic review of cytotoxic zinc, iron, cobalt and copper compounds. The structure – activity relationships and the mechanisms of antitumour action are formulated for each type of metal complexes.The bibliography includes 181 references.

Synthesis, structure and properties of tris(hydroxymethyl)aminomethane complexes with biogenic metal salts

New coordination compounds - analogues of hydrometallatranes, characterized by FT-IR spectroscopy, mass spectrometry and elemental analysis, were synthesized by the interaction of tris(hydroxymethyl)aminomethane (TRIS) with Cu(II), Co(II), Ni(II) and Zn(II) salts of carboxylic acids. The crystal structure of TRIS complex with Cu(II) cinnamate was determined by X-ray diffraction. The structural parameters and gap energy for this complex have been calculated by the DFT method. The thermal analysis data for new TRIS complexes are presented. The effect of some TRIS hydrometallatranes, as well as TEA and TPA hydrometallatranes on the growth of corrosion-aggressive thionic bacteria was studied.

ACCUMULATION OF IRON AND MANGANESE IN THE LEAVES OF PINE PINE AND HERBAL COVER OF THE BESKARAGAY TAPE BORON OF KAZASTAN

The paper presents materials on the study and content of the accumulation of iron and manganese in the woody and herbaceous leaves of the Beskaragai belt pine forest of the dry steppe zone of Kazakhstan, as well as the ratio of iron and manganese to determine the conditions for normal plant life. The results of the research showed that the values of the biotic index in woody and herbaceous plants of the Beskaragay lentchen boron for iron are below 0.3. In the needles of pine trees, none of the zones retained the ratio between iron and manganese for the normal life of pine plants. The accumulation of manganese in herbs occurs mainly from the atmosphere. Of the two biogenic metals, only Mn in the Beskaragai pine forest plays the role of biogeochemical circulation of substances for pine needles. The values of Кн for iron are less than one in pine needles taken from different zones of the Beskaragai forest, which indicates the input of manganese into forest ecosystems, both from the soil and from the atmosphere. The ratio of the concentration of iron and manganese 2: 1, which is necessary for grasses for normal life, is observed only in the zone of unpaved roads of the Beskaragai forest. The work is of great importance for further monitoring of the state of forest ecosystems in the conditions of technogenic and aeronautical pollution of the study area.