Bioelectrochemical Reactor Research Articles

Development, performance evaluation, and kinetic studies of microbial fuel cell based auto dripping bioelectrochemical reactor (AutoDriBER) for urine treatment

ABSTRACT A bioelectrochemical reactor is an assembly, which facilitates energy generation and resource recovery using electrochemically active microorganisms. To maximise energy production from wastewater in this bioreactor system special design is required. Therefore, in the present study, continuous flow auto dripping bioelectrochemical reactors (AutoDriBERs) were developed as a single and multi-electrode assembly for urine treatment. Further, their performance was assessed by connecting reactors in series and parallel arrangements. AutoDriBER configured in series connection showed the highest 93.64 ± 1.57% chemical oxygen demand removal rate with the 1.38 ± 0.64 V voltage and 2.54 W m−3 polarisation power density. The optimum flow rate for maximum voltage production was tested with various models i.e. the linear, exponential, Sweibull-1, and Sweibull-2 models to confirm voltage prediction and its validity. The Linear and exponential models were found best fitted for voltage production with R 2 value of 0.999. These findings infer a novel approach toward optimisation of the complex, inexpensive and self-sufficient design for electricity generation from energy-rich urine wastewater in rural areas.

Effect of Bio-Electrochemical Treatment of Hydroponic Effluent on the Nutrient Content

This study examined the effect of bio-electrochemical treatment processes on nitrogen and phosphorus removal, but it also evaluated the impact of the treatment processes on the concentration of other nutrients present in hydroponic effluent. A bio-electrochemical reactor (BER) was used in the experiment to treat effluent from the hydroponic cultivation of tomatoes. It was stated that the nitrogen removal efficiency decreased with increasing current density. The study showed that an electric current density of 0.63 A/m2 ensured the lowest effluent nitrate concentration. The nitrogen removal efficiency ranged from 41.6%-R1 (density of 0.63 A/m2) to 8.9%-R4 (density of 5 A/m2). Electric current density higher than 1.25 A/m2 resulted in lower total nitrogen removal efficiency. The total phosphorus removal efficiency increased with increasing electric current density. The phosphorus removal efficiency was the lowest—95.1%—in the R1 reactor, whereas it was the highest in R4—99.1%. The concentration of the other elements in the effluent was determined. The content of molybdenum, boron, sulphates, and potassium did not meet the acceptable norms for discharging hydroponic effluent into the environment. The study showed that bio-electrochemical processes taking place in BER caused secondary contamination of hydroponic wastewater with molybdenum ions.

Enhanced nitrogen and carbon removal in natural seawater by electrochemical enrichment in a bioelectrochemical reactor

Municipal and industrial wastewater discharges in coastal and marine environments are of major concern due to their high carbon and nitrogen loads and the resulted phenomenon of eutrophication. Bioelectrochemical reactors (BERs) for simultaneous nitrogen and carbon removal have gained attention owing to their cost efficiency and versatility, as well as the possibility of electrochemical enrich specific groups. This study presented a scalable two-chamber BERs using graphite granules as electrode material. BERs were inoculated and operated for 37 days using natural seawater with high concentrations of ammonium and acetate. The BERs demonstrated a maximum current density of 0.9 A m−3 and removal rates of 7.5 mg NH4+-N L−1 d−1 and 99.5 mg L−1 d−1 for total organic carbon (TOC). Removals observed for NH4+-N and TOC were 96.2% and 68.7%, respectively. The results of nutrient removal (i.e., ammonium, nitrate, nitrite and TOC) and microbial characterization (i.e., next-generation sequencing of the 16S rRNA gene and fluorescence in situ hybridization) showed that BERs operated with a poised cathode at −260 mV (vs. Ag/AgCl) significantly enriched nitrifying microorganisms in the anode and denitrifying microorganisms and planctomycetes in the cathode. Interestingly, the electrochemical enrichment did not increase the total number of microorganisms in the formed biofilms but controlled their composition. Thus, this work shows the first successful attempt to electrochemically enrich marine nitrifying and denitrifying microorganisms and presents a technique to accelerate the start-up process of BERs to remove dissolved inorganic nitrogen and total organic carbon from seawater.

Cooperative cathodes for enhanced hexavalent chromium reduction and electricity generation in bioelectrochemical reactor with simultaneous sludge degradation

A novel bioelectrochemical reactor assembled with cooperative cathodes of chemical cathode and bio-cathode (BERCC) and excess sludge as the anodic substrate obtained continuous and effective Cr(VI) reduction. Cooperative cathodes in BERCC stimulated the growth of electrochemically active microorganisms such as Geobacter sp. and Shewanella sp. in the anodic biofilm and produced 8.21 ± 0.64 mg C/(L·h) more electrons than the dual chemical cathodes in the bioelectrochemical reactor with dual chemical cathodes, which enhanced the electrons for electricity generation and Cr(VI) reduction by approximately 58.3% and 56.1 ± 5.6%, respectively.

Kinetics of Electrochemical Removal of Nickel using Bio-electrochemical Reactor with Packed Bed Rotating Cylinder Cathode

The kinetics of nickel removal from aqueous solutions using a bio-electrochemical reactor with a packed bed rotating cylinder cathode was investigated. The effects of applied voltage, initial nickel concentration, the rotation speed of the cathode, and pH on the reaction rate constant (k) were studied. The results showed that the cathodic deposition occurred under mass transfer control for all values of the applied voltage used in this research. Accordingly, the relationship between concentration and time can be represented by a first-order equation. The rate constant was found to be dependent on the applied voltage, initial nickel concentration, pH, and rotation speed. It was increased as the applied voltage increased and decreased as the initial concentration increased. Its relation to the applied voltage can be fitted as follows: where ko =0.01695 min-1 and -β=0.431. pH and rotation speed have two dissimilar effects on the rate constant. Increasing the pH from 3-6 leads to an increase in the rate constant, while a decrease in the rate constant beyond pH=6 has occurred. Increasing the rotation from 100 to 300 rpm results in an increase in the rate constant. However, the rate constant decreases significantly beyond a rotation speed of 300 rpm.

Rice husk-intensified cathode driving bioelectrochemical reactor for remediating nitrate-contaminated groundwater.

To achieve economical and eco-friendly denitrification, rice husk-intensified cathode driving bioelectrochemical reactor (RCBER) was constructed with rice husk as solid-phase carbon source and microbial carrier. Results demonstrated that the application of current improved the utilization of rice husk and enhanced the denitrification, and the quenching of anodic hydroxyl radicals by rice husk also improved the microbial resistance to current. The highest nitrate removal rate as 0.34 mg-N/(L∙d), higher economic benefits, i.e., current efficiency as 31.6% and energy consumption as 2.43 kWh/g NO3--N, and the highest environmental benefit, i.e., hydrogenotrophic denitrification contribution as 37.9%, were obtained at 200 mA/m2. The best performance at 200 mA/m2 was related to its better microenvironment, such as lower accumulation of anodic by-products and higher bioavailability of rice husks, as well as higher microbial metabolic activity, such as stable extracellular polymeric substance, the maximum electron transport system activity as 11.63 ± 0.14 μg O2·g-1·min-1·mg protein-1 and the highest activity of nitrate reductase (3.15-fold that of control check). The application of current realized the coexistence of heterotrophic and hydrogenotrophic denitrifiers, and multiple functional bacteria such as anaerobic denitrifiers Flavobacterium, aerobic denitrifiers Comamonas, hydrogenotrophic denitrifiers Thermomonas and electron transfer-related Enterobacter coexisted at 200 mA/m2, thereby improving RCBER's adaptability to the complex microenvironment. This study provides the theoretical basis for realizing a win-win situation of environmental pollution remediation and agricultural waste disposal.

Studies on computational fluid dynamics and flow characteristics of auto‐dripping bioelectrochemical reactor (AutoDriBER): A rational basis for e‐urinal design

Studies on computational fluid dynamics and flow characteristics of auto‐dripping bioelectrochemical reactor (AutoDriBER): A rational basis for e‐urinal design

Insights into the syntrophic microbial electrochemical oxidation of toluene: a combined chemical, electrochemical, taxonomical, functional gene-based, and metaproteomic approach

Biodegradation of aromatic hydrocarbons in anoxic contaminated environments is typically limited by the lack of bioavailable electron acceptors. Microbial electrochemical technologies (METs) are able to provide a virtually inexhaustible electron acceptor in the form of a solid electrode.Recently, we provided first experimental evidence for the syntrophic degradation of toluene in a continuous-flow bioelectrochemical reactor known as the “bioelectric well”. Herein, we further analyzed the structure and function of the electroactive toluene-degrading microbiome using a suite of chemical, electrochemical, phylogenetic, proteomic, and functional gene-based analyses.The bioelectric well removed 83 ± 7 % of the toluene from the influent with a coulombic efficiency of 84 %. Cyclic voltammetry allowed to identify the formal potentials of four putative electron transfer sites, which ranged from −0.2 V to +0.1 V vs. SHE, consistent with outer membrane c-type cytochromes and pili of electroactive Geobacter species. The biofilm colonizing the surface of the anode was indeed highly enriched in Geobacter species. On the other hand, the planktonic communities thriving in the bulk of the reactor harbored aromatic hydrocarbons degraders and fermentative propionate-producing microorganisms, as revealed by phylogenetic and proteomic analyses. Most likely, propionate, acetate or other VFAs produced in the bulk liquid from the degradation of toluene were utilized as substrates by the electroactive biofilm. Interestingly, key-functional genes related to the degradation of toluene were found both in the biofilm and in the planktonic communities. Taken as a whole, the herein reported results highlight the importance of applying a comprehensive suite of techniques to unravel the complex cooperative metabolisms occurring in METs.

Development of Bio-Electrochemical Reactor for Groundwater Denitrification: Effect of Electric Current and Water Hardness

Nitrate-nitrogen (NO3-N) contaminating groundwater is an environmental issue in many areas, and is difficult to treat by simple processes. A bio-electrochemical reactor (BER) using copper wire and graphite plate was developed to purify the NO3-N-contaminated groundwater. The low (of 10 mA) and high (of 20 mA) electric currents were applied to the BERs, and various influent hardness levels from 20 to 80 mg/L as CaCO3 due to groundwater characteristics were supplied to clarify the total nitrogen removal efficiency and NO3-N removal mechanisms. In the BER-10, the bio-electrochemical reactions caused 85% of total nitrogen to be removed through heterotrophic and autohydrogenotrophic denitrification in the suspended sludge and biofilm. However, the chemical deposit occurring at the cathode from water hardness affected the decreasing denitrification performance; 12.6% of Mg and 8.8% of Ca elements were observed in the biofilm. The enhancement of electrochemical reactions in the BER-20 caused integrating electrochemical and bio-electrochemical reactions; the NO3-N was electrochemically reduced to NO2-N, and it was further biologically reduced to N2. A better total nitrogen removal of 95% was found; although, a larger deposit of Mg (22.8%) and Ca (10.8%) was observed. The relatively low dissolved H2 in the BER-20 confirmed that the deposit affected the decreasing gaseous H2 transfer and inhibition of autohydrogenotrophic denitrification in the suspended sludge. According to the microbial analysis, both heterotrophic and autohydrogenotrophic denitrification were obtained in the suspended sludge of both BERs; Nocadia (26.8%) was the most abundant genus in the BER-10, whereas Flavobacterium (27.1%) and Nocadia (25.0%) were the dominant genera in the BER-20.

Single-stage or two-stages bio-electrochemical treatment process of drainage from soilless tomato cultivation with alternating current

This work substantially extends knowledge on the possibilities of treating drainage from full-technical scale soilless tomato cultivation. Experiments were carried out in one stage bio-electrochemical reactor and separate biological and electrochemical reactors connected in series, with using alternating current. The study aimed to determine: the effect of single-stage, two-stages wastewater treatment process and hydraulic retention time (HRT) on the effectiveness of contaminant removal and organic substrate consumption, the contribution of biological and electrochemical processes and on the kinetics of contaminant removal. The use of coupled biological and electrochemical processes in one reactor (R1) showed the highest efficiency nitrogen removal, reaching 62.7 % (HRT = 1d) and 89.8 % (HRT = 7d), respectively. The separation of biological (R2-I) and electrochemical processes (R2-II) demonstrated the first one (R2-I) to be mainly responsible for denitrification (TN removal effectiveness at 51.9 % compared to 54.9 % effectiveness of the whole system). In single R1, the concentration of phosphorus in the effluent reached 2.32 ± 0.47 mg P∙L−1 (97.1 %; HRT = 1d) and 1.16 ± 0.06 mg P∙L−1 (98.3 %; HRT = 7d). In R2 enabled reducing phosphorus concentration in the effluent to 0.18 ± 0.07 mg P∙L−1 (99.8 %). The study showed that using of alternating current proved viable in increasing the rate and effectiveness of denitrification, but only if we apply electric current simultaneously with external source of organic carbon.

Effect of bio-electrochemical system on the metabolic changes of Zymomonas mobilis

A bio-electrochemical system can promote the interaction between microorganism and electrode and consequently change cellular metabolism. To investigate the metabolic performance of Zymomonas mobilis in the bio-electrochemical system, we applied an H-type bio-electrochemical reactor to control Z. mobilis fermentation under 3 V. Compared with the control group without applied voltage, the glycerol in the anode chamber increased by 24%, while the glucose consumption in the cathode chamber increased by 16%, and the ethanol and succinic acid concentration increased by 13% and 8%, respectively. Transcriptomic analysis revealed that the pathways related to organic acid metabolism, redox balance, and electron transfer played roles in metabolic changes. Three significantly differentially expressed genes, ZMO1060 (superoxide dismutase), ZMO0401 (diguanylate cyclase), and ZMO1819 (nitrogen fixation protein), were selected to verify their functions in the bio-electrochemical system. Overexpression of ZMO1060 and ZMO1819 improved the electrochemical activity of Z. mobilis. This study provides insights into the microbial metabolism regulated by the bio-electrochemical system.

Technological Parameters of Rotating Electrochemical and Electrobiological Disk Contactors Depending on the Effluent Quality Requirements

Soilless tomato cultivation wastewater, with typically low COD, high concentrations of phosphorus, and oxidized forms of nitrogen, may be effectively treated in a rotating electrochemical disk contactor (RECDC) and in a bioelectrochemical reactor (BER), such as a rotating electrobiological disk contactor (REBDC). The aim of this study was to determine the technological parameters of both reactors, i.e., electric current density (J) and hydraulic retention time (HRT), depending on the effluent quality requirements. The study was conducted with four one-stage RECDCs and with four one-stage REBDCs, at four hydraulic retention times, i.e., 4, 8, 12, and 24 h, and electric current densities of 0.63, 1.25, 2.50, 5.00, and 10.00 A/m2. It was demonstrated that soilless tomato cultivation wastewater could be effectively treated in electrochemical and electrobiological disk contactors, and then discharged to sewage system facilities. In a RECDC, the highest denitrification (53.4%) and dephosphatation (99.8%) performance was achieved at J = 10.0 A/m2 and HRT = 24 h. If the effluents are to be discharged to natural reservoirs, their effective treatment is only feasible in a REBDC. The bioelectrochemical disk contactor ensured over 90% dephosphatation effectiveness. At HRT = 24 h and all electric current densities studied, the concentrations of pollutants in the effluent met requirements set for industrial wastewater discharged into natural waters and the ground. By applying J = 2.5 A/m2 and HRT = 24 h in the REBDC, it was possible to achieve a phosphorus concentration below 3.0 mg P/L and concentrations of ammonia nitrogen and nitrites lower than the permissible levels for treated industrial wastewater introduced to waters and to the ground. Given the nitrate concentration (exceeding 30 mg N/L), an external carbon source is recommended to aid a treatment process that uses a technological system with a REBDC. Technological schemes were proposed for wastewater treatment plants (WWTPs) with a RECDC and a REBDC, for discharging treated wastewater to natural waters, the ground, and sewage systems.

Study the kinetics of electrochemical removal of cobalt from aqueous solutions using a Flow-by Fixed Bed Bio-electrochemical Reactor

Study the kinetics of electrochemical removal of cobalt from aqueous solutions using a Flow-by Fixed Bed Bio-electrochemical Reactor

Bioelectricity Buzz.

Bioelectricity Buzz.

Syntrophy drives the microbial electrochemical oxidation of toluene in a continuous-flow “bioelectric well”

Microbial electrochemical technologies (MET) are promising for the remediation of groundwater pollutants such as petroleum hydrocarbons (PH). Indeed, MET can provide virtually inexhaustible electron donors or acceptors directly in the subsurface environment. However, the degradation mechanisms linking contaminants removal to electric current flow are still largely unknown, hindering the development of robust design criteria.Here, we analysed the degradation of toluene, a model PH, in a bioelectrochemical reactor known as “bioelectric well” operated in continuous-flow mode at various influent toluene concentrations. With increasing concentration of toluene, the removal rate increased while the current tended to a plateau, hence the columbic efficiency decreased. Operation at open circuit confirmed that the bioelectrochemical degradation of toluene proceeded via a syntrophic pathway involving cooperation between different microbial populations. First of all, hydrocarbon degraders quickly converted toluene into metabolic intermediates probably by breaking the aromatic ring upon fumarate addition. Subsequently, fermentative bacteria converted these intermediates into volatile fatty acids (VFA) and likely also H2, which were then used as substrates by electroactive microorganisms forming the anodic biofilm. As toluene degradation is faster than subsequent conversion steps, the increase in intermediate concentration could not result in a current increase.This work provides valuable insights on the syntrophic degradation of BTEX, which are essential for the application of microbial electrochemical system to groundwater remediation of petroleum hydrocarbons.

Performance of a packed-bed anode bio-electrochemical reactor for power generation and for removal of gaseous acetone

The packed anode bioelectrochemical system (Pa-BES) developed in this study is a type of BES that introduces waste gas into a cathode and then into an anode, thereby providing the cathode with sufficient oxygen and reducing the amount of oxygen to the anode to promote the output of electricity. When the empty-bed residence time was 45 s and the liquid flowrate was 35 mL/s, the system achieved optimal performance. Under these conditions, removal efficiency, mineralization efficiency, voltage output, and power density were 93.86%, 93.37%, 296.3 mV, and 321.12 mW/m3, respectively. The acetone in the waste gas was almost completely converted into carbon dioxide, indicating that Pa-BES can effectively remove acetone and has the potential to be used in practical situations. A cyclic voltammetry analysis revealed that the packings exhibited clear redox peaks, indicating that the Pa-BES has outstanding biodegradation and power generation abilities. Through microbial community dynamics, numerous organics degraders, electrochemically active bacteria, nitrifying and denitrifying bacteria were found, and the spatial distribution of these microbes were identified. Among them, Xanthobacter, Bryobacter, Mycobacteriums and Terrimonawas were able to decompose acetone or other organic substances, with Xanthobacter dominating. Bacterium_OLB10 and Ferruginibacter are the electrochemically active bacteria in Pa-BES, while Ferruginibacter is the most abundant in the main anode, which is responsible for electron collection and transfer.

Bioelectrochemical reactor improved by assembling anode with rice husk for treating nitrate-contaminated groundwater

To maximize the hydrogenotrophic denitrification and minimize the negative impact of anode by-products, a rice husk-enhanced anode bioelectrochemical reactor (RABER) was constructed by assembling anode with rice husk, and the denitrification performances and mechanisms involved were investigated. It was demonstrated that rice husk can be used as a slow-release carbon source and microbial carrier in RABER and weakened the anodic by-products. RABER obtained the highest contribution of hydrogenotrophic denitrification as 33.7% and achieved the fastest nitrate removal rate of 0.27 mg-N/(L·d) at 200 mA/m2. The relative lower accumulation of dissolved oxygen (1.99 ± 0.11 mg/L) and hydroxyl radicals were obtained at 200 mA/m2. Moreover, stable extracellular polymeric substances and higher activities of denitrification-related enzymes, i.e., around 2.78-fold for nitrate reductase and 1.96-fold for nitrite reductase those of 0 mA/m2, promoted denitrification in RABER at 200 mA/m2. At an optimal current density of 200 mA/m2, the coexistence of cellulose-degrading bacteria (Cellvibrio), aerobic denitrifier (Brevundimonas), anaerobic denitrifier (Flavobacterium), and hydrogenotrophic denitrifier (Alicycliphilus) was achieved, and the inorganic carbon generated by heterotrophic denitrifiers and electrochemical mineralization was sufficient to meet the demands of hydrogenotrophic denitrifiers, conductive to the heterotrophic‑hydrogenotrophic synergistic denitrification. This study provided a theoretical basis for the enhancement of hydrogenotrophic denitrification in BER as well as minimizing the side effects of the anode and providing a new strategy for the disposal of agricultural waste.

Synergistic effect of bioanode and biocathode on nitrobenzene removal: Microbial community structure and functions

This study aimed to reveal the synergistic effect of bioanode and biocathode on nitrobenzene (NB) removal with different microbial community structures and functions. Single-chamber bioelectrochemical reactors were constructed and operated with different initial concentrations of NB and glucose as the substrate. With the synergistic effect of biocathode and bioanode, NB was completely removed within 8 h at a kinetic rate constant of 0.8256 h−1, and high conversion rate from NB to AN (92%) was achieved within 18 h. The kinetic rate constant of NB removal was linearly correlated with the maximum current density and total coulombs (R2 > 0.95). Increase of glucose and NB concentrations had significantly positive and negative effects, respectively, on the NB removal kinetics (R2 > 0.97 and R2 > 0.93, respectively). Geobacter sp. and Enterococcus sp. dominated in the bioanode and biocathode, respectively. The presence of Klebsiella pneumoniae in the bioanode was beneficial for Geobacter species to produce electricity and to alleviate the NB inhibition. As one of the dominant species at the biocathode, Methanobacterium formicicum has the ability of nitroaromatics degradation according to KEGG analysis, which played a crucial role for NB reduction. Fermentative bacteria converted glucose into volatile fatty acids or H2, to provide energy sources to other species (e.g., Geobacter sulfurreducens and Methanobacterium formicicum). The information from this study is useful to optimize the bioelectrocatalytic system for nitroaromatic compound removal.

Coupling of bioelectrochemical toluene oxidation and trichloroethene reductive dechlorination for single-stage treatment of groundwater containing multiple contaminants

Bioremediation of groundwater contaminated by a mixture of aromatic hydrocarbons and chlorinated solvents is typically challenged because these contaminants are degraded via distinctive oxidative and reductive pathways, thus requiring different amendments and redox conditions. Here, we provided the proof-of-concept of a single-stage treatment of synthetic groundwater containing toluene and trichloroethene (TCE) in a tubular bioelectrochemical reactor, known as a “bioelectric well”. Toluene was degraded by a microbial bioanode (up to 150 μmol L−1 d−1) with a polarized graphite anode (+0.2 V vs. SHE) serving as the terminal electron acceptor. The electric current deriving from microbially-driven toluene oxidation resulted in (abiotic) hydrogen production (at a stainless-steel cathode), which sustained the reductive dechlorination of TCE to less-chlorinated intermediates (i.e., cis-DCE, VC, and ETH), at a maximum rate of 500 μeq L−1 d−1, in the bulk of the reactor. A phylogenetic and functional gene-based analysis of the “bioelectric well” confirmed the establishment of a microbiome harboring the metabolic potential for anaerobic toluene oxidation and TCE reductive dechlorination. However, Toluene degradation and current generation were found to be rate-limited by external mass transport phenomena, thus indicating the existing potential for further process optimization.

Alternative Biological Process for Livestock Manure Utilization and Energy Production Using Microbial Fuel Cells

Carbon-based porous materials are most widely used for Microbial Fuel Cells (MFC) based on their unique properties facilitating and allowing the development of high surface area electrode. The electrochemically active layer of the electrode was prepared using two types of catalysts: activated carbon (Norit NK) and activated carbon promoted with CoTMPP (AC/CoTMPP). Mobilization of phosphate ions in the liquid phase was observed during the process of livestock manure treatment. From 20 mg l−1 initially, the concentration of dissolved phosphates reached 100 mg l−1 after 96 h. Increased concentration of ammonium ions in the medium was also observed, indicating ongoing anaerobic mineralization of the organic matter. The processes taking place in the bio electrochemical reactor used result in recovery of nutrients and production of energy. A maximum current density of 140 μА cm−2 was reached during the MFC operation. The chemical oxygen demand (COD) removal rates were relatively high (above 2 g O2/L/h) for both differently catalyzed cathode configurations. As widely reported elsewhere, the electrochemical results confirm that a gas-diffusion electrode using activated carbon catalyst is very well suited as a positive electrode for use in bio electrochemical systems.