Biodiversity Loss Research Articles

Patterns of aerial arthropod biomass and diversity from three regions of boreal Alaska

Abstract Multi‐decadal monitoring has revealed dramatic evidence of global decline in arthropod biomass and biodiversity, yet little baseline information exists for Alaska, a region 1/5th the size of the contiguous US states. We documented patterns of aerial arthropod biomass and diversity in a multi‐year investigation of three regions in boreal Alaska—two in central Alaska (Fairbanks and Tetlin) and one in southcentral Alaska (Anchorage). Sampling employed canopy Malaise and near‐ground pollinator traps as part of a parallel study of a steeply declining songbird. Traps yielded 115,078 specimens of 15 orders and 692 unique taxa with body sizes ≥3 mm. During peak summer (15 June–15 July), mean aerial arthropod biomass was more than three times higher in traps in Fairbanks (50.6 mg·d−1, confidence interval [CI]: 34.5–63.7) than Anchorage (15.8 mg·d−1, CI: 9.3–26.4). Tetlin exhibited an intermediate value (35.4 mg·d−1, CI: 18.4–67.4). Temperature correlated positively with captured biomass, whereas wind (above 1.5 m·s−1) correlated negatively. To obtain species‐level diversity data, we focused on beetles, representing a wide range of feeding guilds and taxa. Beetles accounted for 6229 adult specimens, 364 unique (mostly species‐level) taxa and 47 families. Trophic categorizations of beetles were similar for the central Alaskan sites; both had a greater proportion of wood‐feeding beetles than Anchorage. Ten species are potentially new to science and 49 are new state records for Alaska. Our work provides the first insight into regional differences and seasonal trends in aerial arthropod biomass and diversity in boreal Alaska, creating important baselines for the future.

Planning for the future: Grasslands, herbivores, and nature‐based solutions

Abstract Global interest and investment in nature‐based solutions (NbS) are rapidly increasing because of the potential of this approach to concurrently counter biodiversity loss, provide cost‐effective measures for climate change adaptations, and maintain natural processes that underpin human health and wellbeing. Recognition is growing that grasslands in many regions will protect carbon stores more effectively than forests in the warmer, drier, more fire‐prone conditions of the future while also serving as hotspots for biodiversity. Yet grasslands have received less attention for their NbS potential. Despite the wide‐ranging goals of this approach, many investments in nature‐based solutions also have focused narrowly on using plants to meet climate pledges, often without considering plant interactions with herbivores and the abiotic environment that jointly control ecosystem functioning and underpin the success of nature‐based solutions. Here, we review the roles that large and small vertebrate and invertebrate herbivores play in the ability of the world's grasslands to provide nature‐based solutions, with a focus on wild herbivore impacts on biodiversity and carbon storage. Synthesis. Planning for nature‐based solutions with a holistic, ecologically informed view that includes the role of herbivores and their interaction with plants and the environment will allow NbS investments to more likely achieve successful, sustainable outcomes.

Safe and genotyped. A non-invasive method for extraction of amphibian DNA from water baths and its application on Northern spectacled salamanders, Salamandrina perspicillata (Savi 1821)

AbstractThe global decline of amphibian populations, with 40.7% of species classified as threatened, calls for innovative and ethical approaches in conservation genetics. Molecular biology advancements have introduced environmental DNA (eDNA) analysis, primarily focusing on aquatic environments. However, the present study explores a novel non-invasive protocol using water samples to extract DNA from terrestrial and semi-terrestrial amphibians, specifically the endangered Italian endemic salamander, Salamandrina perspicillata (Savi, 1821). Unlike traditional invasive methods involving tissue sampling, this protocol immerses animals briefly, eliminating the need for digit or tail amputations or manipulation for buccal swabs. The study validated the protocol through DNA extraction, amplification, and sequencing, yielding results comparable to traditional methods. The non-invasive nature of the protocol aligns with the 3Rs principles (Replace, Reduce, Refine) and offers a streamlined, stress-minimizing alternative for studying protected and endangered species. Future experiments should also explore further refinements, including reduced soaking times and additional applications, such as skin microbiota analysis. This protocol represents a significant step towards ethical and effective research practices in amphibian conservation genetics, encouraging a paradigm shift in wildlife research ethics. Continued innovation in non-invasive methodologies is essential for comprehensive understanding and robust conservation strategies amid the ongoing biodiversity crisis.

Managing Forests for Biodiversity Conservation and Climate Change Mitigation.

We include biodiversity impacts in forest management decision making by incorporating the countryside species area relationship model into the partial equilibrium model GLOBIOM-Forest. We tested three forest management intensities (low, medium, and high) and limited biodiversity loss via an additional constraint on regional species loss. We analyzed two scenarios for climate change mitigation. RCP1.9, the higher mitigation scenario, has more biodiversity loss than the reference RCP7.0, suggesting a trade-off between climate change mitigation, with increased bioenergy use, and biodiversity conservation in forests. This trade-off can be alleviated with biodiversity-conscious forest management by (1) shifting biomass production destined to bioenergy from forests to energy crops, (2) increasing areas under unmanaged secondary forest, (3) reducing forest management intensity, and (4) reallocating biomass production between and within regions. With these mechanisms, it is possible to reduce potential global biodiversity loss by 10% with minor changes in economic outcomes. The global aggregated reduction in biodiversity impacts does not imply that biodiversity impacts are reduced in each ecoregion. We exemplify how to connect an ecologic and an economic model to identify trade-offs, challenges, and possibilities for improved decisions. We acknowledge the limitations of this approach, especially of measuring and projecting biodiversity loss.

Blue Carbon Ecosystems for Climate Resilience in Indonesia: A Study of Adaptation Strategy

Climate change due to global warming will have an impact on marine and coastal ecosystems, including causing loss of biodiversity and threatening the sustainability of marine and coastal resources. The Sixth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC) made Indonesia, which is a tropical archipelagic state as one of the hotspots that is very at risk of being affected by global warming. This risk is especially experienced by cities on the coast. Therefore the importance of adaptation, especially in dealing with the impacts of climate change that has already occurred. The adaptation process cannot be delayed any longer because the earth’s temperature will certainly increase beyond the threshold of 1.5 degrees Celsius in 2030 compared to the pre-Industrial Revolution temperature of 1850. Currently, the global temperature increase has reached 1.1 degrees Celsius. Therefore, Indonesia must immediately make adaptation efforts by utilizing the Blue Carbon Ecosystem which is based on research that blue carbon can absorb and store 100 times more carbon and is more permanent than forests on land. This stored carbon can be stored for thousands of years. Because of this great potential, coastal ecosystems can play many roles as adaptation solutions and mitigation of climate change impacts. Therefore researchers conduct research with the aim of the research is to create Strategies and Adaptation Efforts for Utilizing Blue Carbon Ecosystems: Disaster Resilience, Climate Crisis, and Sustainable Development, with the main target of implementing Resource Based Theory in developing strategies for utilization of Marine Resources, especially Utilization of Carbon Ecosystems Blue in Indonesian marine environment. This research is expected to provide theoretical and empirical evidence related to the development of blue ecosystem utilization strategies, climate crisis, and sustainable development.

U.S. beef producer perspectives on “sustainable beef” and implications for sustainability transitions

Beef production systems are at the center of ongoing discussion and debate on food systems sustainability. There is a growing interest among beef producers, consumers, and other beef supply chain stakeholders in achieving greater sustainability within the industry, but the relationship of this interest to general sustainability issues such as climate change, biodiversity loss, food security, livelihood risks, and animal welfare concerns is unclear. Specifically, there is very little research documenting how beef producers define and view the concept of sustainability and how to achieve it. Producer perspectives are critical to identifying constraints to sustainability transitions or to help build agreement with other producers about the shared values such transitions may support. Through a secondary analysis of survey data of U.S. beef producers (n = 911) conducted in 2021 by the Trust in Food division of Farm Journal, a corporation that provides content, data, and business insights to the agricultural community (e.g., producers, processors/distributors, and retailers), we investigated what “sustainable beef” means to U.S. beef producers, highlighting the key components and constraints they perceive to achieving desirable sustainability outcomes. Leveraging the three-pillar model of sustainability as a framework for analysis, we identified key themes producers use to define “sustainable beef.” We found that producers collectively viewed sustainability as: (1) multidimensional and interconnected; (2) semi-closed and regenerative; (3) long-lasting; and (4) producer-centered, although an integrated perspective uniting these aspects was rare. We discuss how these perspectives may be the basis for sustainability efforts supported by producers and raise future research considerations toward a shared understanding of what sustainability is and what is needed for enduring sustainability solutions in the U.S. beef industry.

An Examination of the Ways in Which Transdisciplinary Research Could Be Used to Incentivize Local Communities to Combat the Illegal Wildlife Trade

The illegal wildlife trade (IWT) is currently one of the most critical conservation concerns, given its direct impact on biodiversity loss, endangering local ecosystems, and adding pressure to all species at a point when they face dangers like deforestation and mass extinctions. This industry also significantly impacts local communities, many of which are compelled to engage in it as a result of their precarious socioeconomic conditions. While effective countermeasures to this global issue have been identified, successful implementation of these countermeasures require diverse disciplines and collaborators. This paper argues that a transdisciplinary approach that converges knowledge and skills from social and socioecological fields of study is required to develop strategies that effectively incentivize communities to combat the IWT. This is true because A) at the social level, criminal justice is needed for the retraining and participation in law enforcement efforts to mitigate support for poaching; B) at the socio-ecological level, environmental economics and ecosystem management are needed for the validation of community assets and profitable management of local wildlife; and C) ecology and business administration are needed for the implementation of conservation initiatives like ecotourism. This paper not only presents the implementation of a transdisciplinary approach but also details the most important disciplines needed, provides a review of the correlation between this industry and the locals who live in close proximity to wildlife and conservation areas, and proposes workable recommendations to supporting initiatives that combat the IWT and serve as pillars for sustainable action. Without adopting and reinforcing the position argued for in this research, the IWT would continue to pose a threat to local ecosystems, populations, and economies. This is a major risk to global sustainability and will exacerbate the loss of biodiversity that is already recognized as an imminent threat by the United Nations Sustainable Development Goals.

The Comprehensive Regeneration Approach as a Framework for Sustainable Development and Biodiversity

Several national and international frameworks have been developed to halt and reverse biodiversity loss by 2030 and help address the ongoing climate and biodiversity crises. These frameworks provide the targets against which locally applicable action plans can be created. The Royal Commission for AlUla and the IUCN have developed Comprehensive Regeneration as the framework of nature-positive initiatives and as a model for the responsible development of AlUla County in the Kingdom of Saudi Arabia.

Anthropogenic Eutrophication Drives Major Food Web Changes in Mwanza Gulf, Lake Victoria

AbstractDiscerning ecosystem change and food web dynamics underlying anthropogenic eutrophication and the introduction of non-native species is necessary for ensuring the long-term sustainability of fisheries and lake biodiversity. Previous studies of eutrophication in Lake Victoria, eastern Africa, have focused on the loss of endemic fish biodiversity over the past several decades, but changes in the plankton communities over this same time remain unclear. To fill this gap, we examined sediment cores from a eutrophic embayment, Mwanza Gulf, to determine the timing and magnitude of changes in the phytoplankton and zooplankton assemblages over the past century. Biogeochemical proxies indicate nutrient enrichment began around ~ 1920 CE and led to rapid increases in primary production, and our analysis of photosynthetic pigments revealed three zones: pre-eutrophication (prior to 1920 CE), onset of eutrophication with increases in all pigments (1920–1990 CE), and sustained eutrophication with cyanobacterial dominance (1990 CE–present). Cladoceran remains indicate an abrupt decline in biomass in ~ 1960 CE, in response to the cumulative effects of eutrophication and lake-level rise, preceding the collapse of haplochromine cichlids in the 1980s. Alona and Chydorus, typically benthic littoral taxa, have remained at relatively low abundances since the 1960s, whereas the abundance of Bosmina, typically a planktonic taxon, increased in the 1990s concurrently with the biomass recovery of haplochromine cichlid fishes. Overall, our results demonstrate substantial changes over the past century in the biomass structure and taxonomic composition of Mwanza Gulf phytoplankton and zooplankton communities, providing a historical food web perspective that can help understand the recent changes and inform future resource management decisions in the Lake Victoria ecosystem.

Universiti Sains Malaysia Campus Nature Challenge: A Collaborative Citizen Science Initiative For Biodiversity Conservation And Environmental Awareness

Considering the growing global biodiversity crisis, citizen science initiatives have emerged as powerful tools for long-term ecological data collection involving non-experts in research. “USM Campus Nature Challenge”, a collaborative effort led by the USM Library in partnership with the School of Biological Sciences and the Centre for Global Sustainability Studies, aimed to create awareness about ecological concerns and biodiversity conservation within the university community by documenting biodiversity at USM’s main campus. Methodologically, the project organized a 6-week ‘bio blitz’ event open to citizen scientists to explore the flora and fauna of USM’s diverse campus habitats while cataloging species through the iNaturalist app. The results revealed that plant species dominated the observations, constituting 64.9% of recorded biodiversity and reflecting the rich botanical diversity on the USM campus. Further observations included insects (10.4%), fungi (7.6%), birds (6.3%), and various other species, underscoring the complexity of the ecological web of the campus. An evaluation of data quality revealed that 36.6% of the species identifications were categorized as “Research Grade”, 47.8% were designated as needing further identification, and 15.7% were graded as “casual”. In conclusion, the project leveraged the approach to create more public awareness about local ecological concerns and to strengthen the University community’s connection to nature. This USM Library’s pilot initiative closely aligns with the Open Science movement related to citizen science in Malaysia.

Interrelationships and Trade-Offs between Urban Natural Space Use and Biodiversity

Urban natural spaces provide important ecosystem services and a wide range of health- and well-being-related benefits for their visitors. They are also essential spaces for biodiversity protection and promotion in a world of rising urbanisation rates and worsening impacts of climate change. However, these spaces are often underutilised by urban residents. When they are utilised, this usage often leads to some level of environmental degradation and biodiversity loss. Hence, understanding how to promote both use and biodiversity levels in urban natural spaces is critical. While various reports have studied the broad factors associated with urban natural space use, the specific relationship between biodiversity and use remains to be explored. This paper uses a Systems Thinking approach to unpack the complex relationship between urban natural space use and biodiversity and to help guide the design and management of these spaces in a way that promotes both use and biodiversity. With data collected from a systematic literature review, a causal loop diagram (CLD) was constructed and analysed. The CLD construction and analysis highlighted various key factors that play an important role in relating urban natural space use and biodiversity. Among these is the role of individual and social perceptions and values in determining how biodiversity levels will affect usage, and vice versa. The results were applied to a case study: the Thamesmead regeneration project undertaken by the social housing association Peabody. We made recommendations regarding Peabody’s biodiversity and green infrastructure plans for Thamesmead, presenting new design and maintenance techniques and assessing various existing techniques mentioned in the documents. Through the CLD analysis, we uncovered various unintended consequences from common design and maintenance techniques and discuss these trade-offs and relationships.

Indigenous Knowledge and Agricultural Practices of Ifugao Ethno-Farming in Ifugao Village, Diffun, Quirino

This study endeavors to delve into the traditional knowledge and farming methods employed by Ifugao farmers residing in Barangay Ifugao Village, Diffun, Quirino. Despite the proven efficacy of Indigenous farming practices, they confront formidable challenges including climate change, biodiversity loss, and the erosion of Indigenous knowledge. The research aims to meticulously document and safeguard the wealth of knowledge and practices upheld by Ifugao farmers, recognizing their pivotal role in fostering environmental sustainability and cultural enrichment. Utilizing an ethnographic approach, this study endeavors to unravel the intricate interplay between farming techniques, traditional wisdom, and the multifaceted challenges posed by modernization. By employing key informant interviews conducted in the Ilocano dialect, the project seeks to elucidate how Ifugao farmers adapt their agricultural practices in response to evolving environmental and socioeconomic dynamics. Through purposive sampling, experienced Ifugao farmers are selected as key informants, ensuring a comprehensive examination of the methods, beliefs, practices, strategies, and knowledge that have underpinned Ifugao ethno-farming traditions. The study, spanning one month and focusing on seasoned Ifugao practitioners, guarantees a thorough exploration of the adaptive measures and customs intrinsic to Ifugao’s agricultural heritage. Reflexive thematic analysis serves as the analytical framework for interpreting the gathered data, with anticipated results offering valuable insights into the resilient techniques employed by Ifugao farmers to preserve their cultural heritage and navigate contemporary challenges. These findings underscore the imperative for collaborative endeavors aimed at safeguarding, documenting, and integrating Indigenous knowledge into agricultural practices, thereby fostering resilience and sustainability for present and future generations. Keywords: Ifugao, Ethno-farming, Indigenous Knowledge, Sustainability, Preservation, Agricultural Practices, Beliefs, Changes perceived, Adaptive Strategies

Creating wildlife habitat using artificial structures: a review of their efficacy and potential use in solar farms.

The biodiversity crisis is exacerbated by a growing human population modifying nearly three-quarters of the Earth's land surface area for anthropogenic uses. Habitat loss and modification represent the largest threat to biodiversity and finding ways to offset species decline has been a significant undertaking for conservation. Landscape planning and conservation strategies can enhance habitat suitability for biodiversity in human-modified landscapes. Artificial habitat structures such as artificial reefs, nest boxes, chainsaw hollows, artificial burrows, and artificial hibernacula have all been successfully implemented to improve species survival in human-modified and fragmented landscapes. As the global shift towards renewable energy sources continues to rise, the development of photovoltaic systems is growing exponentially. Large-scale renewable projects, such as photovoltaic solar farms have large space requirements and thus have the potential to displace local wildlife. We discuss the feasibility of 'conservoltaic systems' - photovoltaic systems that incorporate elements tailored specifically to enhance wildlife habitat suitability and species conservation. Artificial habitat structures can potentially lessen the impacts of industrial development (e.g., photovoltaic solar farms) through strategic landscape planning and an understanding of local biodiversity requirements to facilitate recolonization.

A Land Cover Change Detection Approach to Assess the Effectiveness of Conservation Projects: A Study Case on the EU-Funded LIFE Projects in São Miguel Island, Azores (2002–2021)

A Land Cover Change Detection Approach to Assess the Effectiveness of Conservation Projects: A Study Case on the EU-Funded LIFE Projects in São Miguel Island, Azores (2002–2021)

Towards Sustainable Agriculture: Integrating Agronomic Practices, Environmental Physiology and Plant Nutrition

Sustainable agriculture aims to meet the growing global demand for food without compromising environmental integrity. This review paper explores the integration of agronomic practices, environmental physiology, and plant nutrition as pathways toward sustainable crop production. Intensive agricultural practices have led to significant problems such as soil degradation, loss of biodiversity, and environmental pollution. Recent advancements in plant physiology have enhanced our understanding of plant growth and response to biotic and abiotic stresses. Optimizing crop production in a sustainable manner depends on insights into key processes like photosynthesis, water use efficiency, and nutrient uptake. This review discusses the role of plant growth-promoting microorganisms (PGPMs) as biofertilizers, providing an alternative to harmful agrochemicals. PGPMs can improve soil health and promote plant growth when applied to soil, seeds, or plant surfaces, reducing reliance on synthetic fertilizers and pesticides. Additionally, nanotechnology in agriculture is examined, illustrating how nanomaterials (NMs) and nano-based fertilizers can enhance nutrient absorption and increase crop yields. Beyond these scientific approaches, there is an urgent need to implement novel agricultural practices that support sustainability. The world population is growing at a disquieting rate, placing tremendous pressure on natural resources. This threatens future generations' access to nutritious food and clean air. Innovative approaches like climate-smart agriculture, organic farming, biodynamic agriculture, sustainable intensification, and regenerative agriculture, along with practices such as integrated farming systems, precision agriculture, integrated nutrient management, and integrated pest management, have proven to safeguard agricultural sustainability. By integrating these innovative approaches and practices, it is possible to meet the increasing demands for food while preserving the environment.

Tackling debt, biodiversity loss, and climate change

Experience tells us how to maximize debt-for-nature effectiveness

Power-law productivity of highly biodiverse agroecosystems supports land recovery and climate resilience

Transformative change in primary food production is urgently needed in the face of climate change and biodiversity loss. Although there are a growing number of studies aimed at global policymaking, actual implementations require on-site analyses of social feasibility anchored by ecological rationale. This article reports the in-depth characterizations of low-input mixed polyculture of highly diverse crops managed on the self-organization of ecosystems, which performed better compared to conventional monoculture methods in Japan and Burkina Faso. Analyses on crop productivity and diversity showed that the primary production of ecosystems followed a power law, and through the underlying mechanisms excelled in (1) promoting diversity and total quantity of products along with the rapid increase of in-field biodiversity, especially useful for the recovery of local regime shift in a semi-arid environment; (2) a fundamental reduction of inputs and environmental load; and (3) ecosystem-based autonomous adaptation of the crop portfolio to climatic variability. The overall benefits imply substantial possibilities of a new typology of sustainable farming for smallholders sensitive to climate change, which could overcome the historical trade-off between productivity and biodiversity based on the human-guided augmentation of ecosystems.

Organic food has lower environmental impacts per area unit and similar climate impacts per mass unit compared to conventional

In recent years, interest in studying the climate and environmental impact of organic food has grown. Here, we compared the environmental impacts of organic and conventional food using data from 100 life cycle assessment studies. Most studies focused on climate impacts, with fewer addressing biodiversity loss and ecotoxicity. Findings revealed no significant differences in global warming, eutrophication potential, and energy use per mass unit. However, organic food showed lower global warming, eutrophication potential, and energy use per area unit, with higher land use. Additionally, organic farming showed lower potential for biodiversity loss and ecotoxicity. Challenges in life cycle assessment include evaluating biodiversity, toxicity, soil quality, and carbon changes. The choice of functional units influences results, highlighting the importance of considering multiple units in assessing organic food’s environmental footprint. This study emphasizes the necessity for comprehensive assessments at both product and diet levels to support informed decisions.

UAV and Deep Learning: Detection of selected riparian species along the Ganga River

Abstract. Environmental protection and sustainable natural resource management are being recognised worldwide as essential goals to safe guard human health and well-being. Riparian zones, that face the highest decline in freshwater biodiversity, are of prime conservation priority because they are essential for regulating climate, preserving aquatic-terrestrial biodiversity, maintaining ground water recharge and restoring rivers. In today's fast-paced data-driven environment, artificial intelligence (AI) is the precise answer to a wide range of problems including biodiversity conservation and wildlife management. Leveraging advancements like Uncrewed/Unmanned Aerial Vehicles (UAVs) and AI has resulted innovative strides in wildlife conservation. This study utilised UAV imagery to record high-resolution data of aquatic habitat and species along the Ganga River and employed deep learning algorithms to analyse the data. Through extensive field surveys in the Hastinapur Wildlife Sanctuary, 7,025 photos representing a variety of environments, including 20,000 annotated samples of aquatic animals such as turtles and gharials were generated. Vision based computing capabilities such as pattern recognition model were developed to identify these species. To enrich and enhance the dataset and model, we used different image pre-processing techniques. Slight rotation (±5 degrees), minor cropping (up to 10%), and adjustments in brightness, saturation, and shear (±15%) were applied. Controlled blur (up to 0.5%) and exposure modifications (±5%) were also implemented on the image dataset to improve accuracy. Three Convolutional Neural Network (CNN) architectures, single-stage detectors named YOLO v7, YOLO v8, and Roboflow 3.0, were used for detecting the select species. Results show YOLO v8 excels, achieving mean average precision (mAP) of 98.8% for gharial and 92.2% for turtle detection, with a rapid average detection time of 0.308 seconds per frame at 3200 × 3200 resolution. Additionally, our model demonstrates real-time species detection capability through innovative frame sampling techniques with UAVs. This methodology provides promising technique to collect scientific data on IUCN red listed turtles and critically endangered gharials, allowing detection, monitoring, and real time counting with minimal intrusion. In conclusion, the fusion of UAVs and deep learning promises to revolutionize habitat monitoring, aiding conservation decision-making.

Benefits of tropical peatland rewetting for subsidence reduction and forest regrowth: results from a large-scale restoration trial

Drainage and deforestation of tropical peat swamp forests (PSF) in Southeast Asia cause carbon emissions and biodiversity loss of global concern. Restoration efforts to mitigate these impacts usually involve peatland rewetting by blocking canals. However, there have been no studies to date of the optimal rewetting approach that will reduce carbon emission whilst also promoting PSF regeneration. Here we present results of a large-scale restoration trial in Sumatra (Indonesia), monitored for 7.5 years. Water levels in a former plantation were raised over an area of 4800 ha by constructing 257 compacted peat dams in canals. We find peat surface subsidence rates in the rewetted restoration area and adjoining PSF to be halved where water tables were raised from ~ − 0.6 m to ~ − 0.3 m, demonstrating the success of rewetting in reducing carbon emission. A total of 57 native PSF tree species were found to spontaneously grow in the most rewetted conditions and in high densities, indicating that forest regrowth is underway. Based on our findings we propose that an effective PSF restoration strategy should follow stepwise rewetting to achieve substantial carbon emission reduction alongside unassisted regrowth of PSF, thereby enabling the peat, forest and canal vegetation to establish a new nature-based ecosystem balance.