The bicycle can facilitate both leisure time and transportation physical activity (PA) and therefore its use has the potential to reduce/prevent obesity. The purpose of this study was to explore how household bicycle ownership is associated with different types of PA, sitting time and measures of adiposity. This is a sub-study of the PURE study limited to participants in Vancouver, n=2109. Objective adiposity measures included body mass index (BMI) and waist circumference (WC). Sitting, total, transportation, and recreation PA were assessed using the International PA Questionnaire. Linear regression models were used to assess the association between owning a bicycle with PA and adiposity adjusted for age, sex, marital status, education, income, and ethnicity (significant at p<0.05). Two-thirds of participants had a bicycle in their household and 13.5% reported using a bicycle for cycling specific transportation in the past week. Bicycle owners and non-owners differed in socio-demographic characteristics and PA, but were not different in adiposity measures. After adjusting for potential confounders, ownership was significantly associated with 26%, 36% and 59% more total PA (p<0.001), transportation PA (p<0.05) and recreation PA (p<0.01), respectively, but not associated with sitting time, BMI or WC (p>0.05). Participants with access to a bicycle within their household reported more PA than those without access to a bicycle. Bicycle ownership may be a marker for a more active lifestyle, however, improving access to bicycles (through tax rebates/exclusions and public bike share opportunities to reduce cost) may increase population PA levels.