Benzo[b]fluoranthene Research Articles

Determination of polycyclic aromatic hydrocarbons in Athens atmosphere.

Concentrations of total suspended particulates (TSP), benzene soluble fraction (BSF) and polycyclic aromatic hydrocarbons (PAH) have been determined in ambient air from four different sites in Athens, situated in urban, semi-industrial and industrial areas. GLC analysis has been applied for the determination of PAH, while the CGC/MS technique was used in order to confirm the obtained results. The same PAH pattern was observed for all stations. The identified PAH have been fluoranthene (FLA), pyrene (PYR), benzo(a)anthracene (BaA), chrysene (CHR), benzo(b)fluoranthene (BbF), benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), benzo(e)pyrene (BeP) and benzo(ghi)perylene (B(ghi)P). The concentrations of individual PAH ranged from traces to 33 ng m-3 (e.g. same or lower comparing to other large cities). The higher values of PAH were found during adverse meteorological conditions.

Experimental Studies in Rat Lungs on the Carcinogenicity and Dose-Response Relationships of Eight Frequently Occurring Environmental Polycyclic Aromatic Hydrocarbons<xref ref-type="fn" rid="FN2">2</xref><xref ref-type="fn" rid="FN3">3</xref>

The biologic activity of eight highly purified polycyclic aromatic hydrocarbons (PAH) widely distributed in the human environment was tested in the respiratory tracts of rats. These studies were performed for the examination of carcinogenic activity of the compounds and determination of a dose-response relationship. The lung implantation method was used in 3-month-old female OM rats. A dose-response relationship was obtained for benzo[a]pyrene (BaP), anthanthrene (ANT), benzo[b]fluoranthene (BbF), indeno[1,2,3-cd]pyrene (IND), benzo[j]fluoranthene (BjF), and benzo[k]fluoranthene (BkF). Benzo[e]pyrene and benzo[ghi]perylene showed no tumor-producing effect in this system when given at doses of 5 mg. The histologic and mathematical evaluations indicated that the investigated compounds had distinct carcinogenic potencies. After probit analysis of the results, the carcinogenic potencies of PAH investigated in the lung implantation model rank as follows: BaP, 1.00; ANT, 0.19; BbF, 0.11; IND, 0.08; BkF, 0.03; and BjF, 0.03.

Comparison of toxic and transforming effects of ten environment‐related polycyclic hydrocarbons, including benzo [a] pyrene, on Fetal Hamster Lung Cell Cultures

AbstractStandardized procedures were developed to compare quantitatively the toxic and transforming effects of ten environment‐related polycyclic aromatic hydrocarbons (PAH's) using early passage mixed cultures of fetal Syrian golden hamster lung cells. Evaluation of toxicity was attempted by applying a linear regression to the relative number of surviving colonies. Transformation data were fitted satisfactorily to the one‐hit model. Used as a monitor compound in each subexperiment, benzo[a]pyrene (BaP) showed dose‐related colony reduction (0.01–0.5 μg ml−1). Benzo[b]fluoranthene (BbF), indeno[1,2,3‐cd]pyrene (IcdP), benzo[k]fluoranthene (BkF) and benzo[e]pyrene (BeP) were generally colony enhancing at dose ranges up to 1.0 μg ml−1. Fluoranthene (FLT), pyrene (PYR), chrysene (CHR), benzo[ghi]perylene (BghiPL) and benz[a]anthracene (BaA) were neither stimulatory nor inhibitory to colony formation. For transformation induction, BaP was over 14‐fold more effective than BbF, IcdP or BaA. The other PAH's showed no transformation.