Belonolaimus Longicaudatus Research Articles

Plant-Parasitic Nematode Genera Associated with Turfgrass in Maryland Golf Courses and Athletic Fields.

Field surveys were conducted to assess the occurrence and diversity of plant-parasitic nematodes (PPNs) in golf courses and athletic fields across Maryland, USA, during 2022 and 2023. A total of 28 golf courses and ten athletic fields were surveyed, revealing the prevalence and abundance of 13 PPNs taxa in the region. Criconemoides was identified as the most prevalent (94.9%) and Tylenchorhynchus as the most abundant (2.3) across all samples. Central golf courses (west side of the Chesapeake Bay) exhibited a high prevalence of Criconemoides and Tylenchorhynchus, while Eastern Shore golf courses and athletic fields displayed a higher prevalence of Helicotylenchus and Criconemoides. Further, Belonolaimus longicaudatus was reported for the first time from turfgrass in Maryland, raising concerns due to its potential to cause severe damage on both cool- and warm-season turfgrass. Biodiversity analysis indicated that richness (R2) was higher in athletic fields, while diversity (H') and evenness (J') were significantly greater in golf courses. This study provides baseline information for monitoring PPNs distribution in Maryland and also for the development of effective nematode management approaches in turfgrass ecosystems.

First Report and Molecular Variability of Belonolaimus longicaudatus Associated with Turfgrass in Maryland.

Turfgrass is a crop used extensively in athletic fields and golf courses in Maryland. A soil sample collected in July 2023 from an athletic field in Baltimore County, Maryland, part of a turfgrass nematode survey, contained Belonolaimus longicaudatus. In the southeastern United States, B. longicaudatus is an economically important pathogen of warm season turfgrass. The density was four individuals/100 cm3 of soil, and no visual symptoms were observed in the bermudagrass field. Morphological features and morphometrics of males and females were consistent with B. longicaudatus and placed the Maryland population in a subclade that was geographically represented by populations from north and west Florida, Texas, and South Carolina. Sequencing of the internal transcribed spacer region ITS1 and ITS2 and 28S large ribosomal subunit D2-23 expansion region confirmed the species' identity. Phylogenetic trees and parsimony network analysis placed the Maryland isolate in a large grouping of B. longicaudatus populations including those from Alabama, Delaware, Florida, Indiana, Mississippi, South Carolina, and Texas. To our knowledge, this is the first report of B. longicaudatus in Maryland.

Crop rotation for management of plant‐parasitic nematodes in forage corn production

AbstractCorn (Zea mays L.) is an important silage source for dairy cattle production in the southeastern United States. Silage corn is often grown continuously, which frequently results in severe pressure from plant‐parasitic nematodes such as sting nematode (Belonolaimus longicaudatus). Crop rotation is one of the few nematode management options available in forage production. The research objective was to assess summer‐planted rotation crops, preceding spring silage corn, for management of plant‐parasitic nematodes. Rotation crops included field corn, pearl millet [Cenchrus americanus (L.) Morrone]—with or without fertilizer—, sorghum‐sudangrass [Sorghum bicolor (L.) Moench × Sorghum sudanense Piper], and sunn hemp (Crotalaria juncea L.), which was either incorporated into soil or harvested. Rotation crops were assessed in a 3‐year field study in central Florida. Sunn hemp treatments significantly reduced sting nematode soil abundances relative to corn by 84%, 90%, and 84% before spring corn planting in 2019–2020 and 2020–2021 and at rotation crop termination in 2021–2022, respectively. Abundances of other plant‐parasitic nematodes were not consistently influenced by rotation. Biomass of winter rye (Secale cereale L.)—a bridge cover crop between summer and spring crops—was consistently increased following sunn hemp with significant increases of 87%, 106%, and 186% relative to following corn in 2019–2020, 2020–2021, and 2021–2022, respectively. Similarly, sunn hemp significantly increased spring corn yield by 70% and 136% relative to double‐cropped corn in 2020–2021 and 2021–2022, respectively. Overall, rotation with sunn hemp improved plant‐parasitic nematode management in forage corn production relative to rotations with only gramineous crops.

Belonolaimus longicaudatus management using metam potassium and fluensulfone in potato.

Belonolaimus longicaudatus (sting nematode) is an important pest in Florida potato production and is managed primarily by fumigation using 1,3-dichloropropene (1,3-D). Other effective nematicides are needed for more flexibility in managing this pest. The objective of this study was to evaluate fluensulfone, metam potassium, and mixtures of the two products, relative to 1,3-D and untreated control, for efficacy at managing sting nematode, and for non-target effects on free-living nematodes in potato. To test this objective, a small-plot field experiment was conducted in northeast Florida in 2020 and repeated in 2021. Metam potassium fumigation (390 kg a.i./treated ha)-with or without fluensulfone-managed sting nematode soil abundances but was phytotoxic to potato. Strategies that mitigate metam potassium phytotoxicity, such as reduced application rates, are needed before efficacy of metam potassium in this system can be determined. As a preplant soil spray, fluensulfone alone (403 g a.i./treated ha) did not manage sting nematode abundances and had an inconsistent effect on yield. Fumigation with 1,3-D (88.3 kg a.i./treated ha) was the only treatment that consistently managed sting nematode and increased potato yield. Nematicides did not consistently affect free-living nematodes.

First Report of Belonolaimus Longicaudatus Infecting Soybean in Indiana.

Soybeans (Glycine max) are an important crop for Indiana, playing a major role in the state's economy. In June 2021, symptomatic soybean plants were submitted to Purdue University's Plant and Pest Diagnostic Laboratory for diagnosis. Sting nematodes were observed on the surface of the washed roots using stereo- and brightfield compound microscopy. A total of 76 sting nematodes per 100 cm3 soil were recovered from a composite soil sample. Morphological features and measurements of adult females and males of the sting nematode population were similar to those described for Belonolaimus longicaudatus. Molecular analysis confirmed the morphological identification using D2-D3 expansion segment of the 28S large subunit ribosomal DNA and internal transcribed spacer (ITS) regions. The consensus sequences were submitted to the National Center for Biotechnology Information (NCBI) database with accession numbers OM632679 and OM632681 for the D2-D3 and ITS regions, respectively. Parasitism of the sting nematode population to soybean plants was confirmed as its average population increased from 27 to 40 nematodes per pot 4 wk after inoculation under greenhouse conditions. To the best of our knowledge, this represents the first report of the sting nematode B. longicaudatus in Indiana.

Genome-wide association study and genomic selection for sting nematode resistance in peanut using the USDA public data

ABSTRACT Cultivated peanut (Arachis hypogaea L.) is valued at $1.28 billion annually in the USA. The sting nematode, Belonolaimus longicaudatus, is an ectoparasitic pathogen that can devastate peanut production. Breeding for resistance to this pathogen is one of the most affordable ways to limit its damaging effects on peanut. The use of molecular tools can further accelerate the development of sting nematode-resistant cultivars. The objectives of this study were to identify single-nucleotide polymorphism (SNP) markers associated with sting nematode resistance in peanuts using publicly available datasets and to assess the accuracy of genomic selection in predicting the resistance to sting nematode. SNP discovery was conducted using a genome-wide association study (GWAS) in a peanut panel consisting of 775 USDA accessions, which were phenotyped for sting nematode resistance. GWAS was conducted using 13,306 filtered SNPs, run in TASSEL with three different statistical models. Results showed that (1) a total of 9, 13, 18, 2, and 4 SNPs, respectively, were found to be associated with sting nematode resistance using the single-marker regression, generalized linear model (PCA), generalized linear model (Q), and mixed linear model (PCA), and mixed linear model (Q), respectively; (2) two SNPs overlapped among the models; and (3) genomic selection was higher when the SNPs from GWAS were used to fit the model. To the best of our knowledge, this is the first report on SNP markers associated with and genomic selection for sting nematode resistance in peanuts. These results can be used to develop a molecular breeding strategy to select for sting nematode resistance in peanuts.

Evaluation of a new chemical nematicide, fluazaindolizine (ReklemelTM active), for plant-parasitic nematode management in bermudagrass.

Plant-parasitic nematodes are a major pest of turfgrass in the United States, yet there are few options for successful management. Most current management strategies rely on the use of a limited number of chemical nematicides, so finding a new management option for nematode suppression would be extremely valuable for turfgrass managers. The aim of this study is to evaluate a new nematicide, fluazaindolizine (Reklemel™ active), for its ability to reduce plant-parasitic nematode population density and improve turfgrass quality. Separate research trials were conducted on bermudagrass infested with Belonolaimus longicaudatus and Meloidogyne incognita in greenhouse, microplot, and field settings over 2018 and 2019. Both greenhouse evaluations demonstrated multiple rates of fluazaindolizine reduced B. longicaudatus population density, and one of the two M. incognita trials showed multiple rates of fluazaindolizine reduced nematode population density. Fluazaindolizine was also effective at reducing population density of both B. longicaudatus and M. incognita in microplot settings for both 2018 and 2019, and a significant improvement in turf quality was observed for both visual turfgrass ratings and NDVI. Field trials demonstrated a significant reduction for both B. longicaudatus and M. incognita population density by multiple rates of fluazaindolizine, but no significant differences in turf quality ratings were observed. Overall, fluazaindolizine shows promise as a chemical nematicide for plant-parasitic nematode management on turfgrass.

Southern sting nematode (Ibipora lolii), a serious pest of turf grasses in Australia. A review of what can be learnt from research on Belonolaimus longicaudatus, a closely related pest of turfgrass and many crops in the United States

Sting nematode (Belonolaimus longicaudatus) is one of the most destructive nematode pests in the United States. It is native to the sandy coastal plains of the south-eastern states and causes severe damage to a wide range of plants including turf grasses, ornamentals, forages, vegetables, agronomic crops and trees. Ibipora lolii, a closely related nematode, occurs in Australia and is a widespread and destructive pest of turf grasses, particularly in the Newcastle area of New South Wales and the Perth region of Western Australia. As very little is known about the biology, ecology and control of I. lolii, this review encapsulates the knowledge gained from many years of research on B. longicaudatus and uses it to develop an action plan that, if implemented, would minimise the spread of the nematode and provide more effective control measures.

On-farm evaluations of non-fumigant nematicides on cucurbits

Non-fumigant nematicides were evaluated on cucurbits in two commercial farms in 2017 (Cucumis sativus and Cucumis melo) and three commercial farms in 2019 (Cucumis sativus, Cucurbita pepo and Citrullus lanatus). In 2017, non-fumigant nematicides (fluopyram, oxamyl, fluensulphone, and Majestene) were applied as stand-alone rescue treatments in a cucumber and cantaloupe field heavily infested respectively with Meloidogyne javanica and M. hapla. In 2019, non-fumigant nematicides (fluopyram, oxamyl, and Majestene) were applied both as stand-alone treatments, and as mixtures, in (i) a cucumber field (same as 2017) having a high population of M. javanica, (ii) a squash field previously planted with strawberry and having a high population of M. hapla, and (iii) a watermelon field, previously known to have a high population of sting nematode (Belonolaimus longicaudatus). In 2017, an additional tomato greenhouse study was conducted using the soil from both fields. Both fields in 2017 were re-planted following the rescue nematicide applications, and in the cantaloupe field the new crop established well enough to allow for commercial harvesting. All nematicide treatments in the 2017 cantaloupe field, except for oxamyl, were able to significantly reduce M. hapla root infection after 70 days. Oxamyl did reduce M. javanica root infection in the 2017 cucumber field after 30 days, but the re-planted crop did very poorly, and the field was abandoned after less than one month. In the greenhouse tomato trial, similar trends were observed with better efficacy of the nematicides against M. hapla as compared to M. javanica. In 2019, stand-alone applications of oxamyl and Majestene, and especially the fluopyram + Majestene cocktail significantly reduced the end-season soil population of M. javanica in the cucumber field. No significant effect of any of the products or cocktails was noted on B. longicaudatus in the watermelon field. In the squash field, oxamyl by itself tended to increase soil population of M. hapla, but the mixed application of fluopyram + oxamyl showed some suppression of M. hapla. In both 2017 and 2019 field trials, plant vigor and yield were not significantly affected by the application of any of the nematicides or cocktails. While the nematicides provided better suppression of M. hapla as compared to M. javanica in 2017, the opposite was noted in 2019. This was probably related to the more severe nematode pressure in 2017, especially in the M. javanica field. M. hapla populations tended to decrease during the spring season as soil temperatures increased, while the more tropical B. longicaudatus and especially M. javanica populations increased during the season. Results from this study indicate that although all three nematicides offer an opportunity for cucurbit growers in Florida to help manage nematodes during the cropping season, their efficacy in terms of reducing soil population density or root gall severity, will be dependent on the time of the year, the type of cucurbit that is grown, and the species of nematode present. Some evidence was shown of improved efficacy when nematicide cocktails were applied, but more research is needed to investigate this.

Fluopyram as a rescue nematicide for managing sting nematode (Belonolaimus longicaudatus) on commercial strawberry in Florida

The sting nematode (Belonolaimus longicaudatus) is one of the most economically damaging pests of strawberry in Florida. In this study we evaluated in-season application of fluopyram for management of B. longicaudatus populations at nine commercial strawberry farms. In-crop application of fluopyram reduced B. longicaudatus population densities in soil by the end of the growing season in four out of six of the commercial strawberry farms where B. longicaudatus was detected during the growing season. At the four sites, relative suppression of B. longicaudatus by fluopyram ranged from 54 to 96%. Despite significant suppression of B. longicaudatus population densities in soil, in-crop application of fluopyram did not result in greater strawberry plant vigour by the end of the growing season. Overall, in-crop application of fluopyram shows considerable potential to reduce B. longicaudatus populations on strawberry in Florida.

Yellow and purple nutsedge and coffee senna as hosts of common plant nematodes in Florida.

Yellow (Cyperus esculentus) and purple (C. rotundus) nutsedges, and coffee senna (Senna occidentalis) are common weeds in the southern USA and each have been reported as alternative hosts for plant-parasitic nematodes. Our objective was to determine the host suitability of these weeds to plant-parasitic nematodes common in Florida agriculture and turfgrass systems. The root-knot nematode (RKN) species tested included Meloidogyne arenaria, M. enterolobii, M. floridensis, M. graminis, M. hapla, M. incognita, and M. javanica. The host status of sting nematode, Belonolaimus longicaudatus, was also evaluated, but only on the nutsedge species. All RKN species evaluated reproduced on both nutsedge species and had a reproductive factor greater than one, except for M. graminis on yellow nutsedge. However, only M. hapla, M. javanica, and M. graminis induced visual galls on yellow nutsedge and only M. graminis caused galling on purple nutsedge. Meloidogyne arenaria and M. graminis reproduced at a greater rate on purple nutsedge than on yellow nutsedge. Both nutsedge species were good hosts to B. longicaudatus. Coffee senna was a host to M. enterolobii, a poor host to M. incognita, and nonhost to the other RKN species evaluated.

Plant-parasitic nematodes associated with the root zone of hop cultivars planted in a Florida field soil.

In early 2016, hop plants were introduced into Florida. By late 2016, the hop plants were showing stunted growth and were heavily parasitized by Meloidogyne javanica. In this study, we determined host susceptibility of 14 hop cultivars to M. javanica in a greenhouse experiment and monitored population development of plant-parasitic nematode species in the root zone of 17 hop cultivars planted in three newly established hop yards in Florida. Plant-parasitic nematodes in the rooting zone soil of field grown hop plants included M. javanica, Pratylenchus brachyurus, Paratrichodorus minor, Belonolaimus longicaudatus, Xiphinema setariae/vulgare complex, Mesocriconema xenoplax, and Helicotylenchus dihystera; however, soil population densities of P. minor, B. longicaudatus, X. setariae/vulgare complex, M. xenoplax, and H. dihystera remained low through the study. Root galling, M. javanica egg production, and soil population densities of M. javanica were consistently large on the ‘Canadian Red Vine’, ‘Centennial’, ‘Chinook’, and ‘Comet’ cultivars, and small on the ‘Galena’ and ‘Triple Perle’ cultivars. No differences were observed in soil population densities of P. brachyurus among hop cultivars. Overall, our study provides the first report of plant-parasitic nematode population development in the root zone on hop cultivars planted in Florida.

Plant health evaluations of Belonolaimus longicaudatus and Meloidogyne incognita colonized bermudagrass using remote sensing

The objective of this study was to evaluate the ability of an unmanned aerial system (UAS) equipped with a multispectral sensor to track plant health in the presence of plant-parasitic nematodes in conjunction with nematicide applications. Four nematicides were evaluated for their ability to suppress Belonolaimus longicaudatus and Meloidogyne incognita in microplots, and three nematicides were evaluated on a golf course for their ability to suppress multiple plant-parasitic nematode genera. Visual ratings, Normalized Difference Vegetation Index (NDVI), and Normalized Difference RedEdge Index (NDRE) were reported throughout the trial to assess plant health. B. longicaudatus and M. incognita population density was significantly lowered by nematicide treatments in microplots and correlated with visual ratings, NDVI, and NDRE plant health ratings. On the golf course, all nematicides reduced total plant-parasitic nematode population density at 28, 56, and 84 days after treatment (DAT). Visual turf quality ratings, NDVI, and NDRE were positively correlated with lower nematode population density in the majority of evaluation dates. In the microplot and golf course settings, the parameters evaluated for plant health were correlated with plant-parasitic nematode population density: visual ratings, NDVI, and NDRE improved as nematode population density declined. These results show that remote sensing has the potential to be a beneficial tool for assessing plant-parasitic nematode infected bermudagrass.

First report of the sting nematode Belonolaimus longicaudatus infecting bermudagrass in Barbados.

In 2016, “Tifdwarf” hybrid bermudagrass (Cynodon dactylon (L) Pers. × C. transvaalensis Burtt-Davy) grown on a golf green built to the United States Golf Association recommendations in Barbados started to show irregular significant chlorotic patches followed by gradual thinning and decline of turfgrass. A survey was conducted in May 2016 to determine the presence of plant-parasitic nematodes. The results revealed the presence of the plant-parasitic sting nematode Belonolaimus longicaudatus. To our knowledge, this is the first report of B. longicaudatus associated with bermudagrass in Barbados.

Fusarium wilt of cotton may commonly result from the interaction ofFusarium oxysporumf. sp.vasinfectumwithBelonolaimus longicaudatus

The interaction between Fusarium oxysporum f. sp. vasinfectum (Fov) and Meloidogyne incognita (root-knot nematode) resulting in Fusarium wilt (FW) of cotton is well-known. Although Belonolaimus longicaudatus (sting nematode) can also interact with Fov and cause FW, it has long been believed that virtually all of the FW in Georgia is caused by the interaction of Fov with M. incognita. In recent years, FW has been reported more frequently in Georgia, which suggests that something affecting the disease complex may have changed. In 2015 and 2016, a survey of 27 Georgia cotton fields in 10 counties was conducted. At least 10 soil and stem samples per field were collected from individual plants showing symptoms of FW to quantify plant-parasitic nematode levels and identify Fov races. Fov race 1 was identified in all samples in 2015, but one sample also had the LA110 genotype and another sample also had the LA108 genotype. In 2016, all Fov races and genotypes found in 2015 were present, however, MDS–12 and LA127/140 also were found. Meloidogyne incognita was present in 18% of fields in 2015 and 40% in 2016, whereas B. longicaudatus was present in all fields in 2015 and 75% of fields in 2016. Regardless of whether they occurred separately or together, M. incognita and B. longicaudatus were present, respectively, in 18% and 55% of individual samples in 2015 and 40% and 51% in 2016. However, M. incognita without B. longicaudatus was found in 7% of samples in 2015 and 34% in 2016, whereas B. longicaudatus without M. incognita was found in 45% of samples in 2015 and 44% in 2016. We conclude that Fov race 1 continues to be the dominant race in Georgia and many instances of FW in Georgia may be due to Fov interacting with B. longicaudatus and not M. incognita as previously believed.

Fluensulfone and 1,3-dichloroprene for plant-parasitic nematode management in potato production.

Florida produces 35% of the spring potato (Solanum tuberosum) crop in the USA, but plant-parasitic nematodes suppress yield in the region. The stubby-root nematodes, Paratrichodorus (Nanidorus) spp. and Trichodorus spp., vectors for corky ringspot disease, and sting nematode (Belonolaimus longicaudatus) are among the most damaging nematodes in Florida potato production. Nematicide application is an important component of nematode management in this system, but relatively few nematicides are currently available. Therefore, pre-plant applications of fluensulfone nematicide at various rates (3, 4, 6, and 8 l/ha) and the commercial standard fumigant 1,3-dichloropropene (1,3-D) were tested for management of plant-parasitic nematodes in three field trials from 2016 to 2018. Both fluensulfone, at all rates, and 1,3-D consistently decreased sting nematode abundance relative to the untreated control at harvest. Neither fluensulfone nor 1,3-D affected stubby-root nematode abundances at harvest. Efficacy of fluensulfone and 1,3-D for lesion nematode (Pratylenchus sp.) management varied by year. In 2016 and 2018, fluensulfone at most rates and 1,3-D increased marketable potato yield relative to the untreated control with increases by 49 to 66% and 33 to 55% in 2016 and 2018, respectively. In 2017, fluensulfone at lower rates (3, 4, and 6 l/ha) increased marketable potato yield relative to the untreated control by 41 to 61%, but fluensulfone at 8 l/ha and 1,3-D had similar yields to the untreated control. Results suggest that nematicidal activity of fluensulfone and 1,3-D varies by target nematode with both products effective against sting nematode, ineffective against stubby-root nematodes, and inconsistent against lesion nematode. In conclusion, fluensulfone and 1,3-D are effective options for sting nematode management in Florida potato production.

Description and Identification of Four Species of Plant-parasitic Nematodes Associated with Forage Legumes

Four species of plant parasitic nematodes were present in soil samples planted with forage legumes at Alachua County, Florida, USA. The detected species Belonolaimus longicaudatus, Criconemella ornate, Hoplolaimus galeatus, and Paratrichodorus minor were described in the present study. They belong to orders Rhabditida (Belonolaimus longicaudatus, Criconemella ornate, and Hoplolaimus galeatus) and Triplonchida (Paratrichodorus minor) and to taxonomical families Dolichodoridae (Belonolaimus longicaudatus), Hoplolaimidae (Hoplolaimus galeatus) Criconematidae (Criconemella ornate), and Trichodoridae (Paratrichodorus minor). The identification of the present specimens was based on the classical taxonomy, following morphological and morphometrical characters in the species specific identification keys

Damage Functions for Sting Nematode (Belonolaimus longicaudatus) on Bermudagrass Turf

Sting nematode (Belonolaimus longicaudatus) is one of the most aggressive and damaging parasites of bermudagrass, a widely used turfgrass in the southeastern United States. A damage threshold model for sting nematode on bermudagrass was developed with data collected during a 2‐yr field trial at multiple locations in South Carolina. Soil cores and visual turf quality ratings were collected on multiple sampling dates from plots at each location. Bermudagrass root weight and sting nematode density were determined for each core. A total of 678 soil cores were collected and used for root weight, turf quality, and nematode density regressions. Models discussed in this paper include linear and nonlinear regressions, a segmented regression model, and the first Seinhorst model for sting nematodes on bermudagrass. Results suggest reduced turfgrass quality is detectable before reductions in root weights are observed. Our findings support the use of low action–level thresholds for sting nematodes on bermudagrass, such as those applied in South Carolina (17 nematodes 100 cm–3 soil).

Occurrence of Belonolaimus in Sinaloa, Northwestern Mexico: A New Report on Distribution and Host Range.

The present study reports the occurrence of the genus Belonolaimus in the state of Sinaloa, Mexico, associated with native plants (i.e., Ziziphus amole and Stenocereus alamosensis) in a natural coastal ecosystem. Both morphological and molecular approaches were employed to characterize the Sinaloa population. Notwithstanding of some morphological and morphometric variation between Belonolaimus from Sinaloa and other valid species, the characterization indicates that this population might belong to the Belonolaimus longicaudatus species complex. Molecular analyses based on the 28S gene and ITS1-5.8S-ITS2 regions of the ribosomal RNA (rRNA) identified four major clades within Belonolaimus; however, none of the species including B. longicaudatus, B. gracilis, and B. euthychilus were supported as monophyletic; yet monophyly is argued to be a basic requirement of species status. Sequence divergence among different Belonolaimus populations and species varied according to the rRNA dataset (i.e., ITS1-5.8S-ITS2 > 28S > 18S) used, thus showing the importance of using genes with different rates of evolution to estimate species relationships. The fact that Belonolaimus has not been found in other cultivated (including on suitable hosts) areas in Sinaloa and that this population is relatively distant from the common B. longicaudatus groups (i.e., clades A and B) suggests that its appearance was not due to a recent introduction associated with the local agriculture.

Integrated Pest Management of Nematodes on Bermudagrass Turf

Use of bermudagrass (Cynodon dactylon) cultivars resistant/tolerant to plant‐parasitic nematodes is an important component of integrated pest management (IPM) for sustainable turf nematode management in sandy coastal soils of the southeastern United States. A multiyear field experiment evaluated five bermudagrass genotypes (‘Tifway’, standard susceptible; two commercial cultivars, ‘TifSport’ and ‘Celebration’; and two experimental germplasm, BA132 and PI 291590, that were identified as tolerant to sting nematode [Belonolaimus longicaudatus]) and four different nematicide regimes (untreated control, Curfew Soil Fumigant [1,3‐dichloropropene], monitoring‐based IPM [action threshold nematicide program], and calendar‐based IPM [nematicide rotation]). Nematode samples were collected at 3‐month intervals from February 2012 to November 2014. No significant differences among the genotypes with regard to population density of sting nematode were documented, but there were differences among genotypes with regard to some of the other plant‐parasitic nematodes, particularly spiral (Helicotylenchus paxilli) and ring (Mesocriconema ornatum) nematodes. There were significant nematicide regime responses on nematode population level after August 2013. A calendar‐based IPM program rotating Nortica (Bacillus firmus strain I‐1582), Multiguard Protect (furfural), MustGrow Invest (Brassica juncea), and Avid (abamectin) significantly reduced the nematode population density compared with a monitoring‐based IPM program and the standard soil fumigant nematicide Curfew. In the untreated control, nematode population densities were significantly higher than in the other treatments. Celebration and PI 291590 bermudagrass genotypes had better percent green cover throughout the study.