Bean Extract Research Articles

The Possible Effects of Green Coffee Bean Extract on Progression of Experimental Diabetes in Different Organs; A Review Article

Green coffee bean water extract consumption has been stated to have a contrary association with type 2 diabetes and metabolic syndrome. Chlorogenic acid (CGA), an important biologically active dietary polyphenol, is produced by certain plant species and is a major component of coffee. Reduction in the risk of a variety of diseases following CGA consumption has been mentioned in recent basic and clinical research studies. This systematic review discusses in vivo animal and human studies of the physiological and biochemical effects of CGA on biomarkers of diabetic complication and the possible alterations produced by the administration of different doses of green coffee on biomarkers related to renal, testicular, and neurological antioxidant systems.

Functional and Health Properties of Yogurt Supplemented with Green Tea or Green Coffee Extracts and its Effect on Reducing Obesity Complications in Rats

The chemical composition and sensory evaluation of yoghurt supplemented with green tea (GTE) or green coffee (GCE) extracts and its effect on reducing obesity complications in rats were investigated. Ethanol extracts were prepared from green tea and green coffee and their total phenolic compounds and identified phenolic compounds were evaluated. Results showed that the GTE and GCE have containing highly antioxidant activities. Yoghurt was prepared from low fat buffalo's milk (1.5% fat) blended with 1 % of GTE or GCE. Addition of GTE or GCE had significant effects on the chemical composition and sensory evaluation. Higher sensory scores were reported flavor for GTE and GCE yoghurt than control. It was also noticed that addition of GTE or GCE to yoghurt increased viscosity a key defensive effect on the gel-factors. Feeding of obese rats on yoghurt adding to GTE or GCE showed that significant differences reduced of weight compared to positive group. There are not found significant differences of heart weights tissue while, kidney and liver weights tissues were significant differences. Also, fed obese rats on yoghurt supplemented with GTE or GCE decreased the lipids profile comparative with positive control group and improvement of liver and kidney functions. The best treatment was observed in yoghurt adding to green coffee beans extract which had reduced value of liver and kidney function parameters followed by yoghurt adding to green tea extract. The histopathological examination observed normalized in the groups fed yoghurt supplemented green tea and coffee beans extracts. Supplemented yoghurt with green tea and coffee extracts could be increased the useful health effects of yogurt by intensifying antioxidant activity which had therapeutic effects on obese rats.

Correlation between Proteasome Activity and ECM Component Synthesis and Improvement Effect on Skin Elasticity by Adzuki Bean Extract

プロテアソーム活性は加齢とともに低下する。プロテアソーム活性を阻害すると，正常ヒト皮膚線維芽細胞におけるコラーゲン合成，ヒアルロン酸合成酵素，エラスチンの遺伝子発現が低下することが明らかとなった。この結果は，プロテアソームが細胞外マトリックス（ECM）成分の維持に重要な役割を担っていることおよびプロテアソーム活性の低下は，加齢によるシワの増加やたるみにつながる可能性がある。さらに，私たちはアズキエキスがプロテアソーム活性を亢進することによって肌のハリを改善する効果があることを見出した。アズキエキスは，プロテアソーム活性を亢進し，正常ヒト皮膚線維芽細胞におけるコラーゲン合成とヒアルロン酸合成酵素とエラスチンの遺伝子発現促進効果を有する。また，in vivo試験において顔の弾力とハリ感覚改善の効果も有する。アズキエキスがプロテアソーム活性を亢進させることによってECM成分の合成を促進し，皮膚の弾力性改善効果があることを見出した。

Effects of Bacillus Subtilis-Fermented White Sword Bean Extract on Adipogenesis and Lipolysis of 3T3-L1 Adipocytes.

To investigate the adipogenesis and lipolysis effects of the Bacillus subtilis-fermented white sword bean extract (FWSBE) on 3T3-L1 adipocytes, we treated 3T3-L1 preadipocytes before and after differentiation with FWSBE and measured triglyceride, free glycerol, mRNA, and protein levels. First, FWSBE reduced the cell viability of 3T3-L1 pre-adipocytes under 1000 µg/mL conditions. Triglyceride accumulation in 3T3-L1 pre-adipocytes was suppressed, and free glycerol content in mature 3T3-L1 adipocytes was increased in the FWSBE treatment groups, indicating that FWSBE has anti-obesity effects. Further, FWSBE suppressed adipogenesis in 3T3-L1 pre-adipocytes by lowering the protein levels of C/EBPα, PPARγ, and FAS and increasing the level of pACC and pAMPK. Additionally, FWSBE promoted lipolysis in mature 3T3-L1 adipocytes by increasing the transcription levels of Ppara, Acox, and Lcad and the protein levels of pHSL and ATGL. Thus, we suggest that FWSBE can be a potential dietary supplement because of its anti-obesity properties.

Coffea canephora Bean Extract Induces NIH3T3 Cell Migration

BACKGROUND: Wound healing is an essential biological process that consists of sequential steps aimed at restoring the architecture and function of damaged cells and tissues. There are empirical evidences of using pure coffee bean powder as an alternative medicine in treating various types of wounds. However, there is limited data on coffee-induced wound healing, especially migration of cells. Therefore, current study was conducted to investigate the role of coffee extract in cell migration, especially fibroblast which is important for wound healing.METHODS: Coffea canephora beans were prepared, extracted and added in the NIH3T3 cell culture in final concentration of 2.5% and 5%. Then cytotoxicity test was performed using Na,30-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate (XTT) assay. Meanwhile, cell migration test was performed with scratch assay. All results were statistically analyzed.RESULTS: The 2.5% or 5% Coffea canephora beans extract (CCBE)-treated NIH3T3 cell numbers were almost similar with the numbers of NIH3T3 cells in starvation medium merely. Meanwhile, 2.5% and 5% CCBE showed significant decrease of the widths of scratched areas compared to starvation medium merely (ANOVA with LSD Post-hoc, p=0.000). After 24 h and 48 h, the average widths of 2.5% and 5% CCBE-treated scratched areas were 235.68±22.79, 50.36±5.29, 229.95±23.01, 27.68±2.83, respectively.CONCLUSION: Since both 2.5% and 5% CCBE are potential in inducing migration of fibroblast (NIH3T3 cell) and do not induce cytotoxicity, the CCBE could be potential as an agent for wound healing.KEYWORDS: coffee, Coffea canephora, NIH3T3, migration, cytotoxicity

Exploration of the Potential Mechanism of Faba Bean-Wheat Intercropping to Control Faba Bean Fusarium Wilt Due to Allelopathic Plant Extracts.

We intensively studied faba bean (Vicia faba L.) and wheat (Triticum aestivum L.) intercropping and found that this type of intercropping can effectively control the occurrence of faba bean wilt under field conditions. We conducted hydroponic experiments to explore the role of plant extracts in the process of soil-borne diseases and the mechanism of disease control of faba bean and wheat intercropping. In this experiment, three concentration gradients of faba bean and wheat stem, leaf, and root extracts were added to study the effects of faba bean and wheat extracts on faba bean growth, the physiological resistance of roots, and the growth of Fusarium oxysporum f. sp. fabae (FOF). Faba bean extracts significantly inhibited the growth of faba bean seedlings and the activity of root defense enzymes and significantly stimulated the growth of FOF at high concentrations. Compared with the treatment with faba bean extracts, wheat extracts significantly enhanced the growth of faba bean seedlings, increased the activity of defense enzymes, and inhibited the growth of FOF. Based on these results, we believe that wheat extracts can effectively alleviate the autotoxicity of faba beans and also control the occurrence of faba bean wilt in the field. This provides a theoretical basis for practical intercropping to reduce the damage caused by faba bean wilt.

Kapasitas Total Antioksidan Ekstrak Metanol Biji Kakao (Theobroma cacao. L) Dengan Metode Spektrofotometri Uv-Vis

Antioxidant compounds is one of compounds are needed by the body to reduce free radicals. The ability of antioxidants to reduce free radicals is called antioxidant capacity. The purpose of this study was to provide information on the total antioxidant capacity of the methanol extract of cocoa beans with the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method using spectrophotometer uv-vis. The crude extract obtained was extracted using methanol solvent by maceration method. The results of the phytochemical screening test on the methanol extract of cocoa beans showed the presence of flavonoids and tannins. The total antioxidant capacity test of cocoa bean extract used the standard equivalent value of gallic acid which has been shown to have a strong antioxidant ability to reduce DPPH free radicals at a wavelength of 517 nm. The results of the measurement of total antioxidant capacity in the variation of treatment, namely sun-dried cocoa beans and oven cocoa beans were 27.730 and 42.454 mg/mL GAEAC. Based on the results of this study, it was found that the ethanol extract of oven cocoa beans had the greatest total antioxidant capacity, namely 42.454 mg/mL GAEAC.

Effect of Foliar Application with Faba Bean and Chickpeas Seed Sprout Extract on Growth and Fruiting of Ewaise Mango Trees

An experiment was conducted during two successive seasons 2019 and 2020 on mango trees cv. Ewaise to study the effect of foliar application faba bean and chickpea seed sprout extract at 0.25 to 1% applied three times along each season (at growth start, fruit set after immediately and one month later) either each extract was sprayed. The influence was evaluated through the response of vegetative growth, leaf N, P, K content, yield and some fruit physical and chemical characteristics.

Effect of Arabica Coffee Bean Extract (Coffea arabica) as a Growth Inhibitor of Enterococcus faecalis ATCC 29212

Arabica coffee seeds contain a composition of Caffeine, Chlorogenic acid, Flavonoids, and Trigonelline. The purpose of this study is to test the antibacterial of Arabica coffee seed extract against bacterium Enterococcus faecalis at concentrations of 1,5625%, 3,125%, 6,25%, 12,5%, 25%, 50% and 100%. The extraction method using maceration with solvent 96%. Antibacterial activity test was done by using the Kirby and Bauer diffusion test method. The results showed that Arabica coffee seed extract could provide inhibition starting from 3,125% with an average diameter of inhibition zone of 1,16 mm to the largest concentration of 100% with an average zone diameter of inhibition of 14,6 mm. At the same time, the average diameter of the inhibitory zone of antibiotic ampicillin at a concentration of 1% as a control (+) is 24,6 mm. The results showed that the greater concentration, the greater the inhibitory zones are formed.
 Keywords: Coffea arabica, Enterococcus faecalis, antibacterial, inhibition zone

Evaluating the radioprotective effect of single dose and daily oral consumption of green tea, grape seed, and coffee bean extracts against gamma irradiation

IntroductionThe aim of this study was to investigate and compare the radio-protective effect of green tea, grape seed, and coffee bean extracts in different oral consumption methods in mice. Materials and methodsIn this experimental-quantitative study 150 mice in 15 equally sized groups were used. For each extract, two groups received 200 mg/kg of herbal extracts’ combination for 7 and 30 consecutive days before irradiation, and one group received 800 mg/kg of the extract 2 h before irradiation (3 Gy gamma-rays of Co-60). The similar groups were classified to receive a combination of the plant extracts (green tea, grape seed, and coffee bean). Irradiation without consuming plant extract (irradiated group), and a control group were also devised. Alkaline comet and micronucleus assays were used to investigate the radioprotective effect on mice blood and bone marrow cells, respectively. ResultsConsumption of all plant extracts significantly decreased the radiation damage to blood and bone marrow cells, compared to the irradiated group (p < 0.01), with grape seed extract showing higher protective effect. Continuous daily oral consumption (one week/month) showed a significant higher radioprotective effect compared to single consumption (p < 0.05). Continuous consumption of the combination of the extracts showed a higher radio-protection in comparison to each of the plant extracts (p < 0.03). ConclusionsThe radioprotective effect of continuous consumption (for one week/month) of the plant extracts was greater than single dose. In continuous consumption protocols, we found the synergetic property and higher radioprotective effect of the plant extract combination compared to each one.

Comparing the effects of green tea/ green coffee bean extracts and chlorhexidine gluconate on salivary Streptococcus mutants count in children with early childhood caries

Dental caries is known as a major oral health problem induced by Streptococcus . and Lactobacillus as primary contributing microorganisms responsible for this condition. The aim of this study was to compare the effect of Green tea/ Green coffee Bean Extracts and Chlorhexidine Gluconate on Streptococcus mutans count. The study was conducted among 90 preschool children aged from 4 to 6 years. Statistical analysis was done using the Shapiro-Wilk test, Friedman test, Chi-square test, paired sample t-test, repeated measures analysis of variance (ANOVA) and Mann-Whitney U test at a signi icance level of 0.05. The results demonstrated a signi icant difference in the mean of salivary S. mutans count after administration of CHG (p-value=0.001) and GT (p-value=0.02) between three different times. The study results indicate the bene icial effects of Green Tea/ Green Coffee Bean extracts and Chlorhexidine Gluconate on salivary Strepto- coccus mutans count.

Determination of Caffeine and Hydrogen Peroxide Antioxidant Activity of Raw and Roasted Coffee Beans Around Habru Woreda, Ethiopia Using UV-Vis Spectroscopy.

BackgroundCoffee is a well-known beverage that is widely used around the world. Despite the wide use of coffee in Ethiopia, there is a lack of extensive studies addressing the issues related to the caffeine content and hydrogen peroxide antioxidant activity of varieties of coffee types, particularly in Habru woreda, Ethiopia.ObjectiveThis study aimed to determine the caffeine content and hydrogen peroxide antioxidant activity of raw and roasted coffee beans collected directly from Habru woreda, North Wollo zone of Ethiopia.MethodsThe study was conducted in Bohoro, Girana, and Wurgisa kebeles of Habru woreda, Ethiopia, by collecting 500 g of green beans of Arabica coffee without considering their variety. Then, the collected beans were divided into raw and roasted coffee to perform aqueous and dichloromethane extraction of their caffeine content and hydrogen peroxide antioxidant activity using UV-Vis spectrophotometry.ResultsThe amounts of caffeine in aqueous and dichloromethane extraction were in the range of 124.01−191.27 ppm and 145.15−200.09 ppm in raw and roasted coffees, respectively. Using the IC50 value, the hydrogen peroxide scavenging activity of the aqueous phase coffee bean extracts in Bohoro raw, Bohoro roasted, Wurgisa raw, Wurgisa roasted, Girana raw, and Girana roasted coffee were 32.17 ppm, 11.69 ppm, 26.14 ppm, 3.12 ppm, 24.83 ppm, and 11.06 ppm, respectively, while that of ascorbic acid was 6.91 ppm.ConclusionThe study showed that the highest amount of caffeine in both aqueous and dichloromethane solvent extraction was found in Bohoro’s raw and roasted coffee beans. Also, the amounts of caffeine in all coffee bean samples were safe and the antioxidant activity was excellent. In most of the samples, significant variations in the concentration of caffeine in raw and roasted coffee bean samples were observed in the two extraction solvents.

FORMULASI SEDIAAN LOTION EKSTRAK ETANOL BIJI KOPI ROBUSTA (Coffea cenephora)

Bean robusta coffee contains polyphenol compounds which are useful as antioxidants. Antioxidants can inactivate the development of oxidation reactions, by preventing the formation of free radicals. Robusta coffee bean plant (Coffea canephora) is an ingredient that can be formulated into lotion preparations. Cosmetic lotion aims to remove dead skin cells, brighten the skin and soften the skin. This research is to make lotion preparations from robusta coffee bean extract (Coffea Canephora) . This research uses pure experiment. Robusta coffee beans are extracted by maceration process. The lotion formulation is made with variations of robusta coffee bean powder (Coffea Canephora) with a concentration of 2%, 3%, and 4%. The characteristic test includes organoleptic test, homogeneity test, Ph test, and irritation test. The purpose of this study was to determine the lotion formulation for robusta coffee bean extract (Coffea Canephora). The results of the lotion test had a homogeneous concentration of 2%, 3% and 4%, light brown to blackish brown, with a distinctive smell of coffee. The ph lotion test had a PH of 5 in all formulations on the blank had a PH of 6. The irritation test on the lotion preparation showed no erythema and edema in 5 volunteers. From all the tests carried out, the lotion formulation has good characteristics.

Bio-inspired green synthesis of reclaimable ZnO nanoclusters using Parkia speciosa Hassk pods and its potential photocatalytic removal of water-borne pollutant and antioxidant activities

ZnO nanoclusters (NCs) were synthesized by hydrothermal method following biogenic route using cost-effective, environment-friendly precursor material like the extract of Parkia speciosa Hassk pods. The as-synthesized ZnO NCs were characterized by various techniques, such as transmission electron microscope, powder X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, etc. The biosynthesized ZnO NCs were made of particles of size ranging from 10 to 22 nm with an average size of the particle of 15 nm. XRD and EDX analyses showed that as-synthesized ZnO NCs using Parkia speciosa Hassk or Stink beans extract resulted in the formation of ZnO NCs with high purity. FTIR studies showed the presence of various functional groups of biomolecules on the surface of ZnO NCs and confirm their role as capping and complexing agent in the formation of ZnO NCs. Further, the biosynthesized ZnO NCs were utilized as an efficient reclaimable photocatalyst for the degradation of 98.8% naphthol green B dye from the aqueous phase under UV254 light within 32 min. The morphological and structural study of the reclaimed ZnO NCs confirmed that it was highly stable and could be used up to 6 cycles without losing any significant catalytic activity. Additionally, the biosynthesized ZnO NCs also showed decent antioxidant property with the IC50 value of 109.61 ± 0.23 μg/mL.

Utilization of Coffee Silverskin as Natural Antimicrobial in Staphylococcus aureus Bacteria

The research aims (1) to analyze the antimicrobial inhibition of waste ofcoffee beans (coffee silverskin) from Staphylococcus aureus; (2) Studying thecharacteristics of antimicrobial activity of coffee bean epidermis in Staphylococcusaureus bacteria and (3) Determining the exact concentration of antimicrobial waste ofcoffee beans from coffee beans (coffee silverskin ) on Staphylococcus aureus bacteria.The research method used was an experimental method, which is to conductantimicrobial experiments on coffee silverskin. The design used is CompletelyRandomized Design (CRD) 1 factor, which as a treatment is silverskin coffee extractconsisting of 4 levels of treatment namely 25%, 50%, 75%, and 100%. The parametersobserved in this study were antimicrobial inhibition, antimicrobial characteristics ofcoffee silverskin, and the best antimicrobial concentration of coffee silverskin. Basedon research results that Robusta coffee bean extract can inhibit Staphylococcus aureusbacteria. The antibacterial activity of Robusta coffee bean extract with a concentrationof 75% resulted in an inhibition zone diameter greater than other concentrations of1.22 mm.

Evaluation of Antiplaque and Antimicrobial Activity of Cocoa Bean Extract: An In Vivo Study

Evaluation of Antiplaque and Antimicrobial Activity of Cocoa Bean Extract: An In Vivo Study

Alleviation of fructose-induced Alzheimer's disease in rats by pioglitazone and decaffeinated green coffee bean extract.

Increased fructose consumption is among bad nutritional habits that contribute to increased incidence of neurodegenerative diseases. We proposed that coffee, the most popular beverage worldwide, may protect against the progression of Alzheimer's disease (AD). We investigated the protective potential of decaffeinated green coffee bean extract (GCBE) and the possible potentiation of pioglitazone (PIO) effects by decaffeinated GCBE in fructose-induced AD in rats. Twenty-four rats [12-untreated and 12-pre-treated (for 4weeks) with GCBE] consumed drinking water supplemented with 10% fructose for 18weeks. Twelve of these rats (6-GCBE-untreated and 6-GCBE-pre-treated) were treated orally with PIO starting on the 13th week for 6weeks. Prophylactic administration of GCBE attenuated oxidative damage (increased cortical reduced glutathione and superoxide dismutase activity), while decreased malondialdehyde. It retarded the activation of acetylcholine esterase, increased acetylcholine level in the cortex of fructose-induced AD. It also impeded the upregulation of beta-secretase-1and the accumulation of Aβ plaques that were induced by fructose drinking. With PIO therapy, GCBE showed better effects alleviating oxidative stress and Aβ extracellular plaques formation, while improving cholinergic activity, learning, and memory ability. In conclusions, the consumption of GCBE may protect against the development of AD and delay the progression of AD when given with PIO. PRACTICAL APPLICATIONS: Decaffeinated dietary supplement of green coffee bean extract attenuated the deleterious consequences of fructose-induced Alzheimer's disease in rats. It improved the antioxidant status and cortical cholinergic activity, while hindered the changes responsible for amyloid plaque formation. It also improved the impaired learning and memory. These results, if confirmed by clinical studies, may recommend the consumption of decaffeinated green coffee beans extract as dietary supplement or as a regular beverage to protect against AD in individuals with family history or early signs of AD. With pioglitazone, such dietary supplement improved pioglitazone efficacy and delayed the progression of AD.

Extratos vegetais no controle de brevicoryne brassicae nas brassicáceas

Neste projeto foi efetuado os testes dos compostos aquosos com extrato de fumo com álcool (Nicotiana tabacum), extrato de mamona (Ricinus communis), extrato das folhas da árvore kassod (Senna siamea), para o controle do pulgão no cultivo orgânico do brócolis (Brassica oleracea var. italica) e da couve flor (Brassica oleracea var. botrytis). Onde foi realizado no interior de Reserva/PR. Foi efetivado o teste de três canteiros com 12 plantas de brócolis com o extrato de fumo com álcool, três canteiros com o extrato de mamona, e três canteiro com o extrato de mamona, três canteiro com o extrato da folha da (Senna siamea) e três canteiro de testemunha sem os extratos, as medidas entre plantas foram de 60 cm, os canteiros tiveram 1.20m de largura por 2m de comprimento com espaçamento de 50 cm um canteiro do outro. Onde foram acondicionados em ordem aleatória o local dos canteiros. No inverno foi implantado os brócolis ramoso cultivar Santana e no verão a couve flor cultivar Teresópolis gigante, as duas culturas com o mesmo sistema. Com o término do experimento, obteve-se as variáveis com resultados significativos, para o brócolis tivemos a massa total e controle de insetos tendo o extrato de folha da Senna siamea e extrato de mamona com média superiores, respectivamente, no ponto massa seca e fresca por plantas, o extrato de Senna siamea com eficácia superior com os demais extratos no cultivo do brócolis, na cultura do couve flor o extrato de mamona com eficácia superior no controle de insetos, em relação massa seca da inflorescência e matéria seca da inflorescência tivemos o extrato de fumo com eficácia final, ao final do experimentos em relação a controle de insetos da cultura de brócolis e de couve-flor obteve-se o extrato de mamona com eficácia no controle de insetos. Para uma maior eficiência desses extratos vegetais, recomendam-se estudos mais aprimorados que envolvam diferentes concentrações dos extratos, afim de determinar uma proporção mais adequada, que permita uma maior eficiência e controle desse inseto, o que irá acarretar na redução do uso de inseticidas sintéticos para os produtores, resultados assim na diminuição dos impactos ambientais e custos de produção.

Green synthesis of copper nanoparticles using green coffee bean and their applications for efficient reduction of organic dyes

Low-cost catalysts synthesized by the green chemistry approach have increasing potential applications for removing toxic organic dyes in wastewaters due to the expense consideration and the preference for environmentally compatible materials. In this study, copper nanoparticles (Cu NPs) were successfully prepared through a facile and green method, using the solution of green coffee bean extract as a reducing and stabilizing agent and the green synthesis takes only one step. Experimental results show that the synthesized Cu NPs exhibited excellent stability. The average particle sizes of as-synthesized Cu NPs were 5–8 nm. In the process of reducing amido black 10B (AB-10B), methylene blue (MB), and xylenol orange (XO), we found Cu NPs are effectively catalysts when sodium borohydride (NaBH4) was used as the reducing agent. Then, Cu/green coffee bean powder composites (Cu/GP C) were synthesized in one step to overcome the difficulty to recover Cu NPs in aqueous solution and we used Cu/GP C as a simple simulated catalytic bed for rapidly and efficiently reducing the methylene blue for multiple times. Cu/green coffee bean powder composite (Cu/GP C) could be used as an efficient and easily recyclable catalyst for reduction of organic dyes in industrial wastewaters due to its excellent catalytic activity, low-cost, and reusability.

Karakteristik Fisik Serbuk Ekstrak Buncis (Pheseolus vulgaris L) dengan Variasi Lama Penyimpanan

Research has been carried out on the analysis of physic properties of green bean extract powder (Phaseolus vulgaris L). This study aims to determine the physical characteristics of the bean extract powder that has been stored in a predetermined time variation. The physic properties tested included water content, solubility and organoleptic tests. The results of the sample water content test with storage variations of less than 1, 1, 2 and 3 months respectively were 5.55%, 2.86%, 3.83% and 3.54% and the solubility was 42.67%, 33, 84%, 44.51% and 34.33% for Artificial Gastrid Fluid (AGF) solvents and 31.79%, 35.08%, 43.24 and 34.52% for Water solvents. The results of the organoleptic test showed that the color of the bean extract powder sample with a storage time of ± 2 months was the most preferred by the panelists, Fresh samples had the highest average scores for taste and aroma, of the panelist samples preferred the samples that had been stored for ± 3 months