FTIR Bands Research Articles

Synthesis of l-glutamic acid containing silica gel for the adsorption and immobilization of Zn2+ in water

A porous silica gel with both COOH and NH2 groups was synthesized in one pot by the addition of l-glutamic acid (l-GA) into a synthesis medium consisting of tetraethyl orthosilicate, water, ethanol, and 1-butylamine. The inclusion of l-GA in the as-synthesized product (l-GA-SiO2) was evidenced by FT-IR and TG measurements. The l-GA was chemically bonded with silica as indicated by the shift of FT-IR bands at 1510 and 1400 cm−1 in the spectrum of l-GA to higher wavenumbers of 1540 and 1460 cm−1 in the one of l-GA-SiO2. The TG analysis showed that the l-GA-SiO2 had an increase of weight loss by 5.6% due to the decomposition of the included l-GA which was centered at around 313 °C, suggested by a new peak in the DTG curve. Compared with the one synthesized without l-GA, the l-GA-SiO2 was more aggregated with smaller surface area. However, the latter showed a higher removal efficiency by 17.1% toward zinc ions in water than the former and the adsorbed ions were hardly released again into water from the used adsorbent.

Degradable silk fibroin – Poly (sebacic acid) diacetoxy terminated, (SF-PSADT) polymeric composite coatings for biodegradable medical applications deposited by laser technology

We report here on the bio-polymeric composite coatings deposition for medical applications, demanding adjustable biodegradability, by the Matrix Assisted Pulsed Laser Evaporation, a sterile laser tool technology, tested for degradation, wettability and bio-compatibility preservation.The specific FTIR bands of both materials indicated stoichiometric deposition of composite coatings, and meanwhile suggested the predominant presence of amorphous SF phase, as also validated by XRD, with low intensity maxima emerging from the amorphous matrix. Concerning the PSADT component, XRD clearly evidenced its specific crystalline contribution.The degradation behaviour in simulated body fluid at 37 °C in dynamic regime was studied as first step of applicability in potential sustained controlled release applications (and/or degradable implanted electronics). Weighing measurements of degraded samples confirmed slower degradation tendency with increasing initial PSADT amount. They also revealed approximately zero order SBF-degradation for 1:1 SF-PSADT ratio. The in vitro assays showed uniform spreading of human mesenchymal stem cells on all synthesized coatings.

Preparation and characterization of nanocellulose–polyvinyl alcohol multilayer film by layer-by-layer method

Nanofibrillated cellulose (NFC) and polyvinyl alcohol (PVA) were alternately deposited on a NFC/polyethylene terephthalate (PET) substrate by the use of layer-by-layer (LBL) self-assembly technique. The results reveal that by adding a single PVA layer on the NFC/PET substrate, the surface exhibited more hydrophilic properties showing reduced contact area and excellent surface wettability. Furthermore, the common FT-IR band for a carboxylic group was observed at 1535 cm−1, which indicates that NFC treated by TEMPO oxidation was attached to the surface layer of cellulose mats. Moreover, the 10 layer LBL assembly of the NFC and PVA were spread out into a reticulate structure during the drying process, resulting in a much rougher surface than that of 1 LBL assemble on the NFC/PET film. Finally, the thermal stability of the multilayer films had been significantly improved.

Preparation and characterization of biogenic CaCO3-reinforced polyvinyl alcohol–alginate hydrogel as controlled-release urea formulation

A slow-release fertilizer formulation was prepared by incorporation of urea in polyvinyl alcohol–alginate hydrogel core followed by deposition of calcium carbonate coating using HCO3−/CO32−-rich alkaline cell-free ureolytic culture broth. High urea encapsulation efficiencies were evident in both CaCO3-reinforced (23.48% w/w) and regular (38.71% w/w) hydrogel matrices. Deposition of carbonate over the matrix surface in the reinforced hydrogel was evident from scanning electron microscopy and typical FTIR band near 1465 cm−1 due to C–O stretch of carbonate ion. Aqueous phase release behaviour was not significantly different between the two hydrogel variants. In the soil column, two-stage urea release pattern (i.e. a slow followed by a fast) was eminent that can extend into hours. Therefore, the modified hydrogel for controlled release could be expected to have wide potential application as slow-release fertilizer in agriculture.

In Vitro Enzymatic Digestibility of Glutaraldehyde-Crosslinked Chitosan Nanoparticles in Lysozyme Solution and Their Applicability in Pulmonary Drug Delivery.

Herein, the degradation of low molecular weight chitosan (CS), with 92% degree of deacetylation (DD), and its nanoparticles (NP) has been investigated in 0.2 mg/mL lysozyme solution at 37 °C. The CS nanoparticles were prepared using glutaraldehyde crosslinking of chitosan in a water-in-oil emulsion system. The morphological characterization of CS particles was carried out using scanning electron microscopy (SEM) and Transmission Electron Microscopy (TEM) techniques. Using attenuated total reflectance Fourier transform infrared (ATR-FTIR) and UV-VIS spectroscopy, the structural integrity of CS and its NPs in lysozyme solution were monitored. The CS powder showed characteristic FTIR bands around 1150 cm−1 associated with the glycosidic bridges (C-O-C bonds) before and after lysozyme treatment for 10 weeks, which indicated no CS degradation. The glutaraldehyde crosslinked CS NPs showed very weak bands associated with the glycosidic bonds in lysozyme solution. Interestingly, the UV-VIS spectroscopic data showed some degradation of CS NPs in lysozyme solution. The results of this study indicate that CS with a high DD and its NPs crosslinked with glutaraldehyde were not degradable in lysozyme solution and thus unsuitable for pulmonary drug delivery. Further studies are warranted to understand the complete degradation of CS and its NPs to ensure their application in pulmonary drug delivery.

Structural, thermal, morphological and optical properties of PEO filled with biosynthesized Ag nanoparticles: New insights to band gap study

Abstract In this study new insight to band gap study was presented. The solution cast technique was employed in the present study for the preparation of polymer composites based on polyethylene oxide (PEO), while silver nanoparticles (AgNPs) were synthesized via the green chemistry method. Reduction of silver ions to metallic silver particles was achieved with environmentally friendly and free-of-cost quince leaves. Correlation was established between the outcomes of band gap investigation and XRD analysis. Preparation of PEO:Ag polymer composites involved addition of suspended AgNPs in various concentrations to PEO solution. PEO:Ag composite films were found to be associated with broad absorption peaks due to plasmonic metallic silver particles at 320–780 nm wavelength. The sample incorporating 15 mL of suspended AgNPs is more amorphous, according to the outcomes of XRD analysis. Meanwhile, the FTIR analysis provided categorical evidence for complexation occurring between AgNPs and PEO matrix. The shifting of FTIR bands of PEO:Ag composite films compared to pure PEO is an evidence for interaction among the composite components. The sample melting point was estimated via a thermal investigation. The outcomes of FTIR and XRD analyses were supported by the morphological study. Pure PEO was found to be associated with interconnected spherulites. At 15 mL of AgNPs, there was a decrease in spherulite size, while at 30 mL AgNPs, larger-sized spherulites appeared. Estimation of the optical band gaps of the films required an in-depth investigation of their optical properties. The outcomes of absorption edge study and band gap analysis emphasized that optical dielectric loss is a skilled method for band gap study. Based on theoretical background and experimental approaches, deep insights into band gap behaviour and materials structure are documented.

Mixed arsenic(III) bis(dimethyldithiocarbamato) derivatives with some oxygen and sulfur donor ligands

Seven mixed arsenic(III) bis(dimethyldithiocarbamato) derivatives of type [(CH3)2NCS2]2AsL where, L = OOCC6H5, OOCCH3, OOCC6H4(OH), SC6H5, SCH2COOH, SOCCH3 and S(C6H4)COOH have been synthesized and characterized by various physicochemical [elemental analysis (C, H, N, S & As), molecular weight and melting point determinations], spectral [UV–Vis., FTIR, NMR (1H and 13C)], thermal [TGA and DTG], powder XRD studies. The presence of FT-IR band at 1064–1095 cm−1 due to thiocarbonyl moity and 13C NMR signal in the range 192.56–198.59 ppm due to -NCS2 carbon resonance have indicated anisobidentate mode of bonding of dithiocarbamate group, alongwith this FT far-IR bands observed due to the υ(As–O) and υ(As–S) vibrations in the region 530–532 cm−1 and 302–333 cm−1 respectively, confirm the bonding of arsenic(III) to oxygen and sulfur. Thus, on the basis of results obtained by above spectral techniques, it may be concluded tentatively that, arsenic(III) derivatives (compound 1–7) adopted a distorted octahedral geometry with lone pair of electrons occupying one of the corner of octahedra. Powder XRD studies revealed that these derivatives have nano ranged particle size (5.72 and 16.29 nm) with lower symmetry (monoclinic) crystal system along with unit cell volume (1731 and 1764 Å3). Thermal studies reveal that arsenic(III) bis(dimethyldithiocarbamato) thiosalicylate (compound 7) gives arsenic sulfide as a final decomposition product, which have various potential applications. Antimicrobial screening test showed enhanced activity than free ligands and standard drugs.

The Study of Reused Silica-Titania Catalyst on Biodiesel Production in Various Reaction Times

This study has related to biodiesel production from Waste Cooking Oil (WCO) with reused silica-titania catalyst in various reaction times (2 – 6h). The reused silica-titania catalyst in this study has been obtained by washing the silica-titania waste released from the separation of catalyst and biodiesel product from palm and waste cooking oils in various reaction times. The washing silica-titania named by reused silica-titania has been characterized by DR UV-Vis to confirm the existence of titanium tetrahedral coordination. Meanwhile, the biodiesel product was characterized by FTIR and several properties such as density, flow rate and an acid number have been analyzed in order to study the reusability of silica-titania in biodiesel production. In addition, the results show that the fraction of titanium tetrahedral coordination in reused silica-titania catalyst (1st and 2nd time) was found to be 26.11% and 24.02%, respectively. The optimum reaction time was found to be 3h, which has been used for FTIR characterization to see significant absorption bands in the wavenumber range of 1300 – 1000 cm-1. This range was attributed to absorption bands of C – O ester from triglycerides and C – O from carboxyl group. The main absorption FTIR bands of biodiesel products and WCO are relatively similar. The examinations of biodiesel properties with respect to density, flow rate, and acid number were conducted at optimum reaction time (3h).

Tailoring chemical and physical properties of graphene-added DNA hybrid thin films

While the characteristics of DNA and graphene are well studied, the chemical and physical properties of graphene-embedded DNA and cetyltrimethyl-ammonium chloride-modified DNA (CT-DNA) hybrid thin films (HTFs) have been rarely discussed due to the limited development of fabrication methodologies. Herein, we developed a simple drop-casting method for constructing DNA and CT-DNA HTFs added with graphene nanopowder (GNP). Additionally, we demonstrated their distinct characteristics, such as their structure, elemental composition, spin states and chemical functional groups, binding interactions, vibration/stretching modes by UV–Vis absorption, PL, and electrical measurements. The EDS spectra of GNP-added DNA HTFs showed C, N, O, Na, and P peaks at characteristic energies. Because of the physical adsorption of GNP on DNA, the peak shifts and suppression of the core spectra of O 1s and P 2p were observed by XPS. The intensity variation of Raman and FTIR bands indicated hybrid formation of GNP in DNA and CT-DNA through adsorption, electrostatic interaction, and π–π stacking. UV–Vis absorption and PL spectra showed the considerable influence of GNP in DNA and CT-DNA HTFs. DNA and CT-DNA HTFs with relatively higher [GNP] showed significant increases of current due to the formation of interconnected networks of GNP in the DNA and CT-DNA HTFs.

MICROENCAPSULATION OF OXOLINIC ACID WITH CHITOSAN BEADS

Chitosan microcapsules were synthesized by the method of ionic gelling. The oxolinic acid was encapsulated in chitosan microcapsules with different concentrations, which was corroborated by FT-IR bands of the capsules that are typical of chitosan and the antibiotic. The inclusion of the antibiotic in the microspheres,was determined by means of thermogravimetry since there were two decompositions temperatures corresponding to different compounds.At several concentrations of oxolinic acid chitosan microspheres had different external morphology, by means of SEM was observed that greater concentration of antibiotic exhibited a smoother external texture, being spherical most of them. The solubility of chitosan microspheres was very low in sea and nanopure water, since the protonationat basic pH of the amino group of chitosan is very low, the solubility was lower.The solubilities of chitosan with different concentrations of antibiotic micro-spheres showed a Gaussian tendency of solubility at different acid pH ranges (hydrogels). The optimum pH of solubility of chitosan spheres occured at pH 3.5, what it was observed at the maximum point of the curve.Chitosan microcapsules had different concentrations of oxolinic acid, because release of antibiotic concentration may occur and was measured by the HPLC method. The percentage of encapsulation of the microspheres was high, being all of them greater than 90%. The release of oxolinic acid on chitosan microspheres was faster at pH 5.7, since the release began from the time 1, instead at pH 7.5 was slower, since the release began after 3 times. Chitosan microcapsules had an average dry weight of 1.1 × 10-3 mg.

Synthesis, single crystal analysis and DFT based computational studies of 2,4-diamino-5-(4-chlorophenyl)-6-ethylpyrim idin-1-ium 3,4,5-trihydroxybenzoate -methanol (DETM)

In the present work, co-crystals synthesis of pyrimethamine with 3,4,5-trihydroxybenzoic acid (Gallic acid), its single crystal analysis, spectroscopic data, and computational studies have been accomplished. The single crystal analysis shows that there are CH⋯O, NH⋯N, NH⋯Cl and OH⋯O types hydrogen bonding interactions responsible to stabilizes these chemical structural designs in the form of 3-dimensional (3-D) network. Additional stabilization of this 3-D set-up is attributed by the π-π and CH … π interactions. Furthermore, the computational study was carried out for comparative purpose. Natural bond orbitals (NBOs), frontier molecular orbitals (FMOs), molecular electrostatic potential (MEP) surfaces and FT-IR analysis were performed using DFT/B3LYP/6-311 + G(d,p) level. While, the UV–Vis analysis of DETM was done by using TD-DFT/B3LYP/6-311 + G(d,p) level of theory. NBO study shows non-covalent interactions (NCIs) which are in good agreement with experimental SC-XRD findings. Calculated FT-IR bands are also found to be in good agreement with experimental FT-IR findings. FMOs and UV–Vis results allowed us to explain the vertical transitions and to interpret the nonlinear optical (NLO) properties. Chemical reactivity of DETM was indicated through the color scheme of plotting MEP surfaces. The NLO properties of DETM have been compared to the standard molecule urea.

Spectrophotometric analysis of the fuzzy role of UV irradiation in commercial polyethylene films

Fourier transform infrared (FTIR) in combination with UV/Vis spectrophotometric analysis were performed to study the role of UV irradiation on the commercial samples of pristine high-density polyethylene (HDPE) films. X-ray diffraction data reveals an increase in the percent crystallinity which correlated and fitted linearly with irradiation times. FTIR experimental absorption spectra for commercial (HDPE) sample and other samples that irradiated with UV lamp for different prolonged time intervals shows the maintenance of main pseudo four characteristic bands in their positions with different intensities. Careful analysis of FT-IR bands reveals a clear variation in both band position and intensity for samples that irradiated up to 16 hours. UV/Vis. electronic spectra reveal the presence of charge transfer band in the UV region around 210 nm with a small change in their fundamental absorption edge and optical energy gap without any evidence for any other band till the end of measurements. Absorbance intensity was found to varies in relation to the irradiation time and sample thickness. Obtained data may be interpreted in terms of free radical and oxidation reactions of polyethylene.

Preparation and characterization of nanofibers of polyvinyl alcohol/polyaniline-montmorillonite clay

This work describes the preparation of polyvinyl alcohol/polyaniline-montmorillonite clay (PVA/PANI-OMMT) nanofibers through electrospinning. Initially, for improving the compatibility of the clay towards the organic polymer, the montmorillonite (MMT) was submitted to an organophilization process using hexadecyltrimethylammonium bromide (CH3(CH2)15N(Br)(CH3)3). The PANI-OMMT composite, which was obtained after the in situ polymerization of aniline in presence of the organophilic clay (ratio of ≈1:3 in weight), was then placed in a matrix of PVA (weight ratio of 1:7 of composite:PVA) for the production of the PVA/PANI-OMMT composite fibers through electrospinning. We used several characterization techniques (X-ray diffraction (XRD); infrared absorption spectroscopy (FTIR), ultraviolet visible absorption spectroscopy (UV–Vis), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS)) to analyze the different samples produced. The clay organophilization was confirmed after observing the (001) plane displacement from 5,6° to 4,7° and absorption bands in 2924 cm−1, 2853 cm−1 and 1479 cm−1, as analyzed by XRD and FTIR, respectively. The presence of polyaniline in its emeraldine salt was identified by the presence of the characteristic UV–Vis absorption bands in 360 nm, 430 nm and 822 nm, while the formation of the PANI/OMMT nanocomposite was confirmed by observing the characteristic FTIR bands of each individual component. From the SEM images, we could establish that the resulting nanocomposite mats were composed by uniform fibers with an average diameter of 240 nm and presented good surface quality. We have found that EIS is an efficient technique for the evaluation of changes in the electric properties of the nanofibers after their exposure to vapors of methanol. We suggest that this organic-inorganic composite may find useful applications in the preparation of several devices based on polymers, such as heavy metal filtration membranes and gas sensors.

The wettability of variously treated As42Se58 thin films

Amorphous As42Se58 films were prepared by thermal evaporation from previously synthetized bulk glass. The influence of the physical ageing, annealing and illumination with white and UV light on the wettability of the thin films surface was studied. The changes in wettability (the equilibrium contact angle) of glycerol and formamide were correlated with the surface roughness, the structure disorder manifested itself by the change of parameter B1/2 (slope of the short wavelength absorption edge) or relevant FTIR band width/shape and surface oxidation/hydrolysis. The darkening of the thin films (the red shift of the optical band gap) was observed with the magnitude depending on the treatment.

Green synthesis of cobalt ferrite nanoparticles using Cydonia oblonga extract: structural and mössbauer studies

Cobalt ferrite nanoparticles (CFNPs) were obtained by a green synthesis method using the aqueous extract of Cydonia oblonga from two different precursors – cobalt nitrate and cobalt acetate. CFNPs were characterized by XRD, SEM, EDS, FT-IR and Mossbauer spectroscopy to estimate cationic distribution. The XRD analysis indicates that the crystallite size of CFNPs is about 8 nm. SEM revealed agglomerated morphology of CFNPs. The force constants KT and KO have been calculated from FT-IR band frequencies and the comparison with the interatomic distances has discussed. The cationic distribution was established from Mossbauer data and it was shown that the use of cobalt acetate as precursor causes more invertible structure of CFNPs.

Amber in prehistoric Iberia: New data and a review.

Provenancing exotic raw materials and reconstructing the nature and routes of exchange is a major concern of prehistoric archaeology. Amber has long been recognised as a key commodity of prehistoric exchange networks in Europe. However, most science-based studies so far have been localised and based on few samples, hence making it difficult to observe broad geographic and chronological trends. This paper concentrates on the nature, distribution and circulation of amber in prehistoric Iberia. We present new standardised FTIR analyses of 22 archaeological and geological samples from a large number of contexts across Iberia, as well as a wide scale review of all the legacy data available. On the basis of a considerable body of data, we can confirm the use of local amber resources in the Northern area of the Iberian Peninsula from the Palaeolithic to the Bronze Age; we push back the arrival of Sicilian amber to at least the 4th Millennium BC, and we trace the appearance of Baltic amber since the last quarter of the 2nd Millennium BC, progressively replacing Sicilian simetite. Integrating these data with other bodies of archaeological information, we suggest that the arrival of Baltic amber was part of broader Mediterranean exchange networks, and not necessarily the result of direct trade with the North. From a methodological perspective, thanks to the analyses carried out on both the vitreous core and the weathered surfaces of objects made of Sicilian simetite, we define the characteristic FTIR bands that allow the identification of Sicilian amber even in highly deteriorated archaeological samples.

Honey mediated microwave assisted sol–gel synthesis of stabilized zirconia nanofibers

Aim of the present work is to prepare zirconia nanofibers using microwave assisted sol–gel method. Both honey and microwave powers are employed as structure directing agents to improve the stability and reduce the crystallite size. Honey, acting as capping agent, prevents the particles from hard agglomeration. Soft agglomeration or less agglomeration results in smaller crystallite size that prevents the transformation of tetragonal to monoclinic phase resulting in stabilized tetragonal zirconia (t-ZrO2). Zirconium oxychloride is used as precursor of zirconium and deionized water as solvent. Effect of microwave powers, in the range of 100–900 W with interval of 200 W, on zirconia stabilization is observed. X-ray diffraction analysis shows the presence of phase pure t-ZrO2 at low microwave power ~ 100 W with crystallite size ~ 26 nm. Formation of phase pure t-ZrO2 at low microwave power is due to the presence of sufficient amount of honey to coat the zirconia crystals. Relatively higher x-ray density has been observed in case of phase pure t-ZrO2 at 100 W of microwave power. This high density and phase purity reveals the high value of hardness (~ 1503 HV). Scanning electron microscopy analysis reveals the formation of well-separated nanofibers without agglomeration at 100 W. These nanofibers are purposed for bone implants and bone grafting. Structural transformation along with hard agglomeration is observed with increase in microwave powers from 500 W to 900 W. FTIR and Raman fundamental tetragonal bands, appearing at 490 cm−1 and 148 cm−1, respectively, confirm the formation of t-ZrO2 at low microwave power. Sample with phase purity exhibits high grain boundary resistance (1.95 MΩ) along with high dielectric constant (~ 74) and low tangent loss (at log f = 4.0). It is worth mentioning here that phase pure t-ZrO2 at very low microwave power (~100 W) with high density and well-separated nanofibers has been obtained without any post heat treatment.

Alkaline Stability of Polyaniline Synthesized Using Pulsed Inductively Coupled Plasma Device

The stability of polyaniline synthesized using pulsed inductively coupled plasma device was studied in alkaline media. Polyaniline was exposed to potassium hydroxide at 25°C and 90°C for various time intervals up to 4 months. An increase in %mass loss, the decreases in the absorbance intensities of polyaniline characteristic bands in FT-IR and UV-Visible spectra and the reduction of the conductivity with increasing exposure time to alkaline solution and/or temperature indicated the increases in the conversion of polyaniline into its base form and the degradation of its structure. The results also suggested that the temperature was the main factor affecting its conductivity in alkaline stability since exposing at 25°C and 90°C caused the conductivity to decrease from 4.53x10-8 S.cm-1 to 1.74x10-8 S.cm-1 and 8.15x10-9 S.cm-1 in 4 months, respectively. Therefore, this polyaniline synthesized via this system may be suitable for the applications at room temperature where alkaline stability is needed.

2D-COS-FTIR analysis of high molecular weight poly (N-vinyl carbazole) undergoing phase separation on purification and thermal annealing

The effect of purification and annealing on the molecular structure of commercial poly (N-vinyl carbazole), PVK, has been probed by two-dimensional Fourier Transformation Infrared correlation spectroscopy, 2D-COS-FTIR. Subtle changes in the spectra of PVK were determined and correlated with changes observed to the multiple glass transition temperatures and the phase separation behaviors. Bands at 1626, 1600, 1575, 1482, 1454, 1332 and 1224 cm−1 were assigned to syndiotactic rich chain segments and decreased on annealing. While bands at 1616, 1590, 1550, 1478, 1444, 1322, and 1218 cm−1 were assigned to isotactic rich segments, and increased on annealing. The commercial PVK consisted of some sections of the polymer chains, which were predominantly syndiotactic and others isotactic. The predominantly isotactic sequences were insoluble and separate on thermal annealing into two phases with different compositions. 2D-COS-FTIR enabled the assignment of the FTIR bands that were related to change in tacticity.

Synthesis of bone implant substitutes using organic additive based zirconia nanoparticles and their biodegradation study

Zirconia, a hard-ceramic, is potential material for bone and dental implants. However, the problem limiting its application is inertness. This problem can be minimized using body compatible and non-toxic organic additives. Organic additives-based zirconia (OZ) nanoparticles are synthesized using sol–gel method. Zirconium oxychloride is used as precursor and water as solvent. OZ nanoparticles are calcined in the temperature range of 100–1000 °C. Transition from mixed zirconia phases to amorphous behavior is observed at 300 °C. Phase-pure tetragonal ZrO2 (t-ZrO2) along with reduced crystallite size ~12.7 nm is observed at 500 °C. Mixed phases, appearing at 800 °C, exhibit increased monoclinic to tetragonal ratio at 900–1000 °C. SEM images show OZ nanoparticles with ~50 nm diameter at 500 °C. Nanoparticles with ~50 nm and ~70–75 nm diameter along with nanowires (~8 nm) are observed at 600–700 °C. FTIR band at 500 cm−1 along with shoulder at 580 cm−1 and Raman band at 148 cm−1 confirm the presence of t-ZrO2 at 500–600 °C. High value of hardness, ~15 GPa, and dielectric constant (~57 68) suitable for bio-application is observed for OZ nanoparticles calcined at 500 °C. Optimized t-ZrO2 is immersed in stimulated body fluid for 1, 2, 4, 8, 13, 20 and 26 weeks. Small degradation in weight and hardness is observed even after 26 weeks of immersion.