Abstract
Herein, the degradation of low molecular weight chitosan (CS), with 92% degree of deacetylation (DD), and its nanoparticles (NP) has been investigated in 0.2 mg/mL lysozyme solution at 37 °C. The CS nanoparticles were prepared using glutaraldehyde crosslinking of chitosan in a water-in-oil emulsion system. The morphological characterization of CS particles was carried out using scanning electron microscopy (SEM) and Transmission Electron Microscopy (TEM) techniques. Using attenuated total reflectance Fourier transform infrared (ATR-FTIR) and UV-VIS spectroscopy, the structural integrity of CS and its NPs in lysozyme solution were monitored. The CS powder showed characteristic FTIR bands around 1150 cm−1 associated with the glycosidic bridges (C-O-C bonds) before and after lysozyme treatment for 10 weeks, which indicated no CS degradation. The glutaraldehyde crosslinked CS NPs showed very weak bands associated with the glycosidic bonds in lysozyme solution. Interestingly, the UV-VIS spectroscopic data showed some degradation of CS NPs in lysozyme solution. The results of this study indicate that CS with a high DD and its NPs crosslinked with glutaraldehyde were not degradable in lysozyme solution and thus unsuitable for pulmonary drug delivery. Further studies are warranted to understand the complete degradation of CS and its NPs to ensure their application in pulmonary drug delivery.
Highlights
IntroductionN-acetylglucosamine (GlcNAc) units linked by β-(1→4) glycosidic bonds
Chitosan (CS) is a linear polysaccharide composed of repeating glucosamine (GlcN) andN-acetylglucosamine (GlcNAc) units linked by β-(1→4) glycosidic bonds
FTIR spectra were obtained of the original CS powder and glutaraldehyde crosslinked CS NPs that were incubated in 0.2mg/mL lysozyme solution at 37 ◦ C
Summary
N-acetylglucosamine (GlcNAc) units linked by β-(1→4) glycosidic bonds. It is an amphiphilic polymer obtained by deacetylation of naturally occurring chitin, which has been extensively studied for the delivery of various drugs, vaccines, genes and chemotherapeutic agents [1,2,3]. Genipin), chitosan micro/nanoparticles are prepared by various techniques. CS micro/nanoparticles have been investigated as carriers have been demonstrated to enhance mucoadhesiveness or cellular absorption upon for lungpulmonary delivery [4,5,6,7].drug delivery Recently,. CS micro/nanoparticles investigated as carriersinterest for pulmonary epithelial layer and large surface area that interest enablesowing therapeutic benefits delivery [2,8,9,10,11,12].
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