Rapid urbanization and population growth in the upper Athi River basin in Kenya have increased the strain on the sub catchments water supply and sanitation situation. Due to increasing demand, inadequate supply of drinking water and lack of sanitation facilities, people in the sub-catchment are increasingly reliant on groundwater as a primary or supplementary water source. However, the use of on-site wastewater systems and agricultural pollution, mainly from runoff containing fertilizers, pesticides, herbicides, and faeces, pose a threat to groundwater in the sub catchment. Subsequently, the current study was conducted in the Thiririka sub catchment, Kiambu County, Kenya, to determine the safety of groundwater sources and to examine the factors influencing groundwater quality in the catchment area. This study assessed the influence of anthropogenic activities on the physical, chemical, and bacteriological quality of groundwater in the Upper Athi River basin of Kenya between April and June 2022. Twenty variables were analyzed and compared with water quality standards to determine hydro chemical characteristics, evidence of contamination, and suitability of groundwater. Shallow wells (SW) had higher concentrations of major ions and key parameters than boreholes (BH), such as alkalinity (7%), turbidity (96%), nitrates (92%), sulfates (48%), phosphates (93%), chlorides (77%), potassium (84%), sodium (30%) and fecal coliforms (99%) significant at p < 0.01. Concentrations of eleven water quality variables however were comparable in both systems. Farming, animal husbandry, and pit latrines were negatively but significantly correlated with the water quality of SW explaining substantial amounts of variation (≤ 45%) in concentrations of water quality variables. Ionic and coliform levels increased with decreasing distance and vice versa. IDW interpolation maps were generated in ArcGIS software to determine the spatial variability of groundwater quality in the basin. Anthropogenic activities such as pit latrines and animal husbandry impaired the quality of groundwater which in most cases was not potable.
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