The irreversible losses such as the flow loss and axial heat conduction loss in traditional porous regenerators have limited the specific power and thermal efficiency of Stirling engines to a great degree. Thereby, the objective of this paper is to design and optimize a novel annular constructal bifurcation regenerator for diminishing the flow and axial heat conduction losses in the regenerator based on constructal concept and response surface methodology. First, the bifurcation type structure units with an inclined angle to the flow direction and the layered approach were applied to construct the regenerator matrix. Thereafter, the numerical simulations were employed to investigate the gas flow and heat transfer characteristics of the constructal bifurcation regenerator. Based on the numerical data, the quadratic polynomial regression models between the geometric parameters and the regenerator performance were established using response surface methodology, and the specific effects of the geometric dimensions were analyzed via 2-D and 3-D response surface plots. Subsequently, a multi-objective optimization was conducted to minimize the flow resistance and maximize the heat transfer rate by genetic algorithm. A most eclectic solution with the global friction factor f = 3.478 and the number of heat transfer units NTU = 1.135 was selected from the optimal Pareto front. Finally, the overall performance of the constructal bifurcation regenerator was compared with that of the porous regenerator and the parallel geometry regenerator. The results indicate that a low flow resistance, low axial heat conduction, and high thermal performance are obtained by the constructal bifurcation regenerator. Therefore, it is reasonable to conclude that the annular constructal bifurcation regenerator achieves a high comprehensive performance, with the potential to improve the performance of Stirling engines. The findings of this paper may provide some new ideas and guidelines for the design and optimization of the regenerator structures.