Objective: Hypertension as a cardiovascular disease occurs due to an uncontrolled increase in blood pressure. Night shift nurses with more overweight, short sleep duration, and excessive stress levels are at risk of increase blood pressure. This study aims to analyze how the relationship between obesity, nutritional status, sleep duration and stress level influence the blood pressure of the night shift nurses.Materials and methods: The subjects in this study were night shift nurses in four hospitals. The dependent variable was blood pressure and the independent variables were nutritional status, sleep duration, and stress levels. This study was an observational analysis with a perspective cohort design in which the subjects were 312 night shift nurses. Nutritional status were identified from Body Mass Index (BMI) through anthropometric measurement, sleep duration by looking at average hours of sleep during the night service, stress levels through the Perceived Stress Scale (PSS-10) questionnaire. Blood pressure was measured using a mercury sphygmomanometer. Data were analyzed by Chi-square test and Logistic Regression.Results and Discussion: There was a significant relationship between nutritional status, sleep duration, and stress levels with blood pressure. The results of the multivariate analysis showed that the shift nurses with overweight (obesity) nutritional status are at a risk of having disorder 1.97 times, the shift nurses with sleep duration < 6 hours are at risk of having disorder 3.78 times and shift nurses with intermediate stress level at risk of having disorder 2.08 times with enhancement blood pressure.Conclusion: There is a relationship between nutritional status, sleep duration and stress level with blood pressure. Sleep duration mostly influences the blood pressure.International Journal of Human and Health Sciences Vol. 04 No. 01 January’20 Page : 55-59