BackgroundNeuroprotection is a developmental supportive approach designated for accelerating brain maturation and to prevent neurological and behavioural problems in newborns. This study assessed the healthcare professional's level of knowledge regarding neuroprotective environment. Materials and methodsA descriptive cross-sectional design was used to determine the knowledge level. The study was conducted among 62 health care professionals in Father Muller Medical College Hospital, Mangaluru, Karnataka, India. The tool consisted of baseline proforma and structured knowledge questionnaire with 28 multiple choice questions validated by 6 experts. Purposive sampling technique was used to select the samples. ResultsThe findings of the study showed that majority (74.2%) of the health care professionals had average level of knowledge with the mean and standard deviation of 14.8 ± 3.63. The knowledge score was maximum in the area of ‘healing environment’ (60.17%) and least for ‘optimizing nutrition’ (36.25%). ConclusionOur findings revealed the need for intensive teaching and training of physicians and nurses for the successful implementation and practice on neuroprotective environment in clinical set-up.