Cd Availability Research Articles

Physiological responses of Morus alba L. in heavy metal(loid)-contaminated soil and its associated improvement of the microbial diversity.

Woody plants have considerable application potential in the phytoremediation schemes, owing to their long-lived large biomass and prosperous root systems in heavy metal(loid)-contaminated soil. Under greenhouse conditions, the physiological response characteristics and phytoremediation possibility of Morus alba L. and its associated improvement of the bacterial and arbuscular mycorrhizal fungal (AMF) diversities in heavy metal(loid) co-contaminated soils were investigated. The results showed that the cultivated M. alba L. plant exhibited significant tolerance against the heavy metal(loid)s in co-contaminated soil and that the microbial diversities were improved notably. The contents of malondialdehyde (MDA) in M. alba L. leaves decreased with cultivation from 90 to 270 days, while the superoxide dismutase, peroxidase and catalase activities were maintained at normal levels to eliminate the production of lipid peroxides. The chemical compositions (e.g. amino acids, carbohydrates and proteins) in the root of M. alba L. fluctuated slightly throughout the cultivation period. Meanwhile, Cd, Pb and Zn were majorly concentrated in the M. alba L. roots, and the maximum contents were 23.4, 7.40 and 615.5 mg/kg, respectively. According to the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis results, the influence of M. alba L. on the rhizosphere AMF community was greater than that on the bacteria community. Meanwhile, the bacterial and AMF Shannon diversity indexes in the contaminated soil were enhanced by 18.7-22.0% and 7.14-16.4%, respectively, with the presence of M. alba L. Furthermore, the correlations between the availability of As, Cd, Pb, and Zn and Shannon diversity indexes of the bacterial and AMF communities were significantly (p < 0.05) positive with the phytoremediation of M. alba L. Therefore, M. alba L. can be suggested as a potential plant candidate for ecological remediation and for simultaneously improving the activity and diversity of microorganisms in contaminated soils.

Effect of amendments on soil Cd sorption and trophic transfer of Cd and mineral nutrition along the food chain.

Phytotoxicity of cadmium (Cd) and its trophic transfer along a terrestrial food chain have been extensively investigated. However, few studies focused on the role of amendments on the trophic transfer of Cd and related mineral nutrients. In a 60-day pot experiment, soil Cd availability, accumulation of Cd, mineral nutrients (Ca and Si) in lettuce, and subsequent trophic transfer along the lettuce-snail system were investigated with or without 3% (w/w) soil amendment (biochar or micro-hydroxyapatite, μHAP). Soil CaCl2 extractable Cd (CdCaCl2) contents decreased by both amendments. μHAP amended soil increased the Freundlich sorption capacity of Cd2+ to a greater extent (15.9mmol/kg) than biochar (12.6mmol/kg). Cd, Ca and Si accumulation in lettuce tissues (roots and shoots) varied with amendment species and soil Cd levels. Linear regression analysis showed that root Cd contents are negatively correlated with root Ca and Si contents (r2=0.96, p<0.05). But no significant correlation between shoot Cd and lettuce Ca and Si contents was found (p>0.05). After 15 days snail feeding, nearly 90% content of Cd was found in snail viscera, while nearly 95% content of Ca was found in snail shells. Contents of Si distributed equally in snail tissues. Biomagnification of Cd, Ca and Si (TF>1) was found in lettuce shoot - snail viscera system. Opposite tendency of TF variation between Cd and nutrient elements (Ca and Si) from shoots to snail tissues indicated that μHAP, rather than biochar, amendment is applicable to remediate soil Cd contamination in our study.

Achieving the safe use of Cd- and As-contaminated agricultural land with an Fe-based biochar: A field study

A field study was conducted to investigate the effect of Fe-based biochar application on the extractability and availability of Cd and As, as well as its impact on crop growth and yield under a two-years wheat-rice rotation system. The Fe-based biochar was applied to the soil at 1.5 and 3.0 t ha–1, manure compost was also applied as a comparison, as well as a non-treated control. The application of the Fe-based biochar significantly (p < 0.05) increased the crop yields for the rice season in the first year, but the both treatments had no significant effect on the crop yields in the others cultivation seasons, compared to the control. The concentrations of available Cd and As significantly (p < 0.05) decreased after either higher or lower dose of Fe-based biochar addition, especially with lower rate in the second year. In the second year, the soil extractable Cd and As reduced by 57% and 18%, respectively, in the wheat season and 63% and 14%, respectively, in the rice season, after the lower dose of Fe-based biochar was applied. The lower dose of the Fe-based biochar treatment showed higher efficiency for decreasing Cd and As availability in soil than the higher one, the control and manure compost treatment. Additionally, both the higher and lower doses of the Fe-based biochar treatments significantly decreased Cd and As uptake by wheat and rice plants. Overall, the Fe-based biochar showed effective immobilization at an application of 1.5 t ha–1, making the use of the Fe-based biochar feasible as an amendment for the safe use of agricultural land contaminated by Cd and As.

Environmental availability and oral bioaccessibility of Cd and Pb in anthroposols from dredged river sediments.

Dredging and disposal of sediments onto land sites is a common practice in urban and industrial areas that can present environmental and health risks when the sediments contain metallic elements. The aim of this study was to characterise and study the environmental and toxicological availability of Cd and Pb in anthroposols from dredged river sediments. To do this, 67 surface samples spread over 12 sediment disposal sites in northern France were studied. The results showed substantial heterogeneity for this matrix in terms of physicochemical parameters and contamination degree; however, ascending hierarchical clustering made it possible to classify the samples into eight groups. For each group, the mobile fraction of Cd and Pb was studied using single EDTA extraction, solid-phase distribution was analysed with sequential extractions and toxicological availability was assessed with the oral bioaccessibility test. The results showed that (i) Cd had a higher environmental and toxicological availability than Pb; (ii) this availability depends on the physicochemical characteristics of the matrix; and (iii) it is necessary to take into account the environmental and toxicological availability of contaminants when requalifying these sites in order to propose appropriate management measures. In the first years after sediment disposal, it would appear that the environmental and toxicological availability of Cd and Pb increased (from 52.5 to 71.8% and from 28.9 to 48.9%, respectively, by using EDTA and from 50.2 to 68.5% for Cd with the bioaccessibility test). Further studies would therefore be required to confirm this trend and understand the mechanisms involved.

Assessment of heavy metal toxicity in soils contaminated by a former Pb–Zn mine and tailings management using flotation process, Jebel Ghozlane, Northern Tunisia

The present work aims to investigate the toxicity of heavy metals in soil samples collected from the vicinity of the former Pb–Zn mine of Jebel Ghozlane and to evaluate reprocessing performances of mine tailings. The research was undertaken using two ecotoxicity tests: (1) MetPAD™ biotest Kit and (2) standardized germination test using wheat seeds (Triticum durum). Water extraction test was carried out to evaluate the potential availability of Pb, Zn and Cd in soil samples. Although their total levels in soil were found to be high, their water extracted values were relatively low (Pb 7–100 µg L−1, Zn 15–238 µg L−1 and Cd 1–5 µg L−1). The high germination percentages (GPs) of wheat seeds indicate that all soil samples tested are not toxic. However, the toxicity of soil–water extracts, as determined by MetPAD™ biotest which is specific for heavy metal toxicity, showed that two of six soil samples exhibited moderate toxicity. The toxicity was related to the relatively high concentrations of water-extractable Zn, Pb and Cd in these two samples. Laboratory flotation tests were used to evaluate the separation ability of Pb and Zn minerals from the mine tailings materials. Results showed that this method allows satisfactory reduction of Pb (95% recovery) and, to a less extent, of Zn (42% recovery) from mine tailings, that could be used as a mitigation strategy for heavy-metal contaminated soil.

Hematite enhances the immobilization of copper, cadmium and phosphorus in soil amended with hydroxyapatite under flooded conditions

Hydroxyapatite (HA) is often applied as chemical amendment in soils contaminated with trace metals such as copper (Cu) and cadmium (Cd). Large amounts of iron oxides in red soil may interacts with phosphate released from HA and influence trace metal immobilization of HA. Here we simulated a red paddy soil with 1–5% wt iron oxides by adding hematite and evaluated the Cu and Cd availability in soil amended with HA under flooded conditions. Changes in phosphorus and iron oxide fractions were also evaluated after a 42-day flooding incubation experiment. Results showed that the addition of HA-only and hematite-only decreased soil redox potential and increased pore water pH compared to the control. HA combined with hematite could effectively decrease phosphate, Cu and Cd in soil pore water compared to HA-only. Additionally, HA combined with hematite could also increase soil pH and decrease soil CaCl2-extractable Cu and Cd. In particular, HA combined with 5% hematite was most effective in reducing soil exchangeable fractions of Cu and Cd by 53.7% and 65.6% compared to the control, respectively. The addition of HA-only increased water-soluble phosphorus, NaHCO3-extractable inorganic phosphorus, NaOH-extractable inorganic phosphorus, and HCl-extractable phosphorus. Conversely, HA combined with hematite treatments decreased NaHCO3-extractable inorganic phosphorus by 11.3–43.0% compared to HA-only. Vivianite and metal–phosphate precipitates were not observed using the Visual MINTEQ model, X-ray diffraction, and chemical analysis. The addition of hematite with or without HA increased free and crystal iron oxide fractions, while it substantially enhanced amorphous iron oxides in the soil. Thus, this study indicates that soil with high hematite content could enhance Cu and Cd immobilization while decreasing phosphorus availability in the red paddy soil amended with HA under the flooded conditions.

Arsenic, lead and cadmium concentrations in caudal crests of the yacare caiman (Caiman yacare) from Brazilian Pantanal

Wetlands are environments of extreme importance due to their high biodiversity and invaluable ecosystem services. Nevertheless, wetlands worldwide are under the increasing threat of the effects of contaminants, which put at risk the biota and the ecosystems. Herein the concentrations of non-essential and toxic elements arsenic (As), lead (Pb) and cadmium (Cd) in caudal crests of the yacare caiman (Caiman yacare) from the Brazilian Pantanal, one of the largest wetlands in the world, were investigated aiming to compare concentrations in individuals from a large pristine area (Southern Pantanal) to animals from an area close to potential sources of anthropogenic emissions (Northern Pantanal). Levels above the limit of detection were found for all the elements in the majority of the analysed samples. The highest mean concentration was detected for As in samples from both sites, followed by Pb and Cd in samples from Southern Pantanal, and Cd and Pb in samples from Northern Pantanal. Significant negative correlations were found between As and Cd concentrations and the size of the individuals from the Southern Pantanal. Surprisingly, concentrations of all three elements were higher in pristine Southern Pantanal, but with significant differences only for Cd. This result suggests that natural sources and processes may be acting for the mobilisation and availability of As, Pb and Cd for Southern Pantanal biota, which reflected in the contamination of C. yacare. Data from the literature showed higher levels of As, Pb and Cd in abiotic compartments in Southern Pantanal and adjacent plateaus in comparison to Northern Pantanal, corroborating the higher concentrations detected in biotic samples from the south. Thus, natural sources seem to play a relevant role in the contamination of biota by As, Pb and Cd in Southern Pantanal, characterising an intriguing pattern that could be tested for other species.

Effects of wheat straw derived biochar on cadmium availability in a paddy soil and its accumulation in rice

This study was carried out to investigate the effect of biochar amendment on cadmium (Cd) availability in a paddy soil with biochar amendment and its effect on the accumulation in rice. Biochar was applied once at rates of 0 (A0), 10 (A10), 20 (A20), 30 (A30), and 40 (A40) t ha−1 on the soil surface layer (0–17 cm). Results showed that the soil organic matter (SOM) content and pH in 0–17 cm soil layer increased as biochar application rate increased, whereas the content of dissolved organic carbon (DOC), and available iron (Fe), manganese (Mn), aluminum (Al) in diethylenetriamine pentaacetic acid (DTPA) extracts declined with biochar added. Available Cd in DTPA extracts in the 0–17 and 17–29 cm soil layer of A40 treatments was significantly lower (p < 0.05) by 49.4 and 51.7% than that in A0. Compared with A0, the distribution factor (DF) of DTPA extracted Cd in the 0.053–0.25 mm and <0.053 mm aggregates of A40 treatments increased by 136 and 269%, respectively, and the DF values in these micro-aggregates of A40 treatments were greater than 1.0. Based on European Community Bureau of Reference (BCR) sequential extraction results, 40 t ha−1 rate of applied biochar reduced the proportion of acid extractable Cd fractions in both 0–17 and 17–29 cm soil layers, but increased the Cd in the oxidizable and residual fractions. The Cd concentration in the rice plants of different biochar treatments was in the order of A0>A10 > A20 > A30 > A40. DTPA extractable Cd concentration in soil was the key factor affecting the Cd uptake by rice roots. In conclusion, biochar application at 40 t ha−1 can effectively reduce the availability of Cd in soil profile, enhance the available Cd enrichment in micro-aggregates, and thus limit the Cd uptake by rice.

Nitrogen combined with biochar changed the feedback mechanism between soil nitrification and Cd availability in an acidic soil

Inorganic nitrogen (N) inputs increase soil nitrification, acidification and trace metal toxicity e.g. cadmium (Cd). Biochar (B) has been widely used for metal immobilization. However, little is known about how the combination of N fertilizers with biochar (N-B) changes soil Cd availability through altering nitrification process. Here, (NH4)2SO4 or CO(NH2)2 was applied in combination with biochar to an acidic, artificially enriched Cd contaminated soil. Not as we expected, available Cd did not increase following (NH4)2SO4 or CO(NH2)2 addition. Nitrification and acidification of Cd contaminated soils were greatly inhibited, accompanied by elimination of ammonia-oxidizing bacteria (AOB). Exchangeable H+ of Cd contaminated soils was significantly lower than that of uncontaminated soils, thus inhibiting Cd itself from mobilization. N-B addition nearly halved soil available Cd and significantly increased nitrification by promoting AOB recovery. However, the restored nitrification did not cause soil acidification, due to the high buffering and slow liming effects of biochar. Available Cd continuously decreased with decreasing soil acidity and exchangeable Al. This study firstly demonstrated a feedback between soil nitrification and Cd after N application, and how biochar modified the feedback. Biochar, therefore, provides a feasible strategy for eliminating potential Cd toxicity on both soil biological and chemical processes.

Physicochemical features, metal availability and enzyme activity in heavy metal-polluted soil remediated by biochar and compost

Biochar and compost have been widely used for pollution remediation of heavy metals in soil. However, little research was conducted to explore the efficiency of biochar, compost and their combination to reduce heavy metals availability, and the effects of their additive on soil biological properties are often neglected. Therefore, this study investigated the effects of biochar, compost and their combination on availability of heavy metals, physicochemical features and enzyme activities in soil. Results showed that adding amendments to polluted soil significantly altered soil properties. Compared to the separate addition of biochar or compost, their combined application was more effective to improve soil pH, organic matter (OM), organic carbon (TOC) and available potassium (AK). All amendments significantly decreased the availability of Cd and Zn, but slightly activated As and Cu. In addition, soil enzyme activities were activated by compost and inhibited by biochar, but exhibited highly variable responses to their combinations. Pearson correlation analysis indicated that electrical conductivity (EC) and AK were the most important environmental factors affecting metal availability and soil enzyme activities including dehydrogenase, catalase, β-glucosidase, urease, acid and alkaline phosphatase, arylsulfatase except for protease and invertase. Availability of As, Cu, Cd and Zn affected dehydrogenase, catalase and urease activities. These results indicated that biochar, compost and their combination have significant effects on physicochemical features, metals availability and enzyme activities in heavy metal-polluted soil.

Effects of biochar amendment on the availability of trace elements and the properties of dissolved organic matter in contaminated soils

Abstract The effects of biochar on the amount and characteristics of dissolved organic matter (DOM) and the relationship between the DOM and the availability of trace elements in soils are poorly understood. An incubation experiment was used to investigate the effects of biochar on DOM properties and the mobility and availability of As, Cd, and Pb in two types of contaminated soils (vegetable plot vs. paddy). The availability of trace elements in the soil was assessed by diffusive gradients using the thin film (DGT) technique. The results showed that mixing biochar at a 10 wt% dose increased the amount of dissolved organic carbon (DOC) from 12.8 to 42.9 mg L−1 in soils, and this corresponded to an increase in soil pH. Fluorescence excitation–emission matrices (EEMs) indicated that rice straw biochar (RSBC) amendment to soil changed the DOM composition towards higher aromaticity. The DGT-extractable Cd and Pb concentrations were significantly decreased with biochar amendments, while DGT-extractable As was significantly increased RSBC treatment decreased acid-soluble Cd and Pb fractions but increased the acid-soluble As fraction in soil solutions compared to control samples. RSBC amendment can effectively adjust soil metal availability, as well as DOM amount and composition, and that the amendment conditions must be tailored according to the specific trace elements and soil types.

Hyperaccumulation of Cd by Rorippa globosa (Turcz.) Thell. from soil enriched with different Cd compounds, and impact of soil amendment with glutathione (GSH) on the hyperaccumulation efficiency

Rorippa globosa (Turcz.) Thell. is known as Cd hyperaccumulator, however neither hyperaccumulation nature, nor affecting factors like the effect of Cd compounds entering soil from different sources, or of specific soil amendments, are not yet satisfactorily clarified. In the pot culture experiment, Cd accumulation by R. globosa from soils spiked with 3 and 9 mg Cd kg−1 in the form of Cd(NO3)2, CdCl2, CdBr2, CdI2, CdSO4, CdF2, Cd(OH)2, CdCO3, Cd3(PO4)2, CdS and effect of soil amendment with glutathione (GSH) were investigated. Accumulation capacity of R. globosa for Cd appeared to reflect its extractability in soils and was about two-fold bigger for high soluble compounds than for low-soluble ones. At that, the differences between the accumulation of Cd originating from high soluble compound group did not exceed 20%, while the differences within the low soluble compound group were insignificant (p < 0.05). The analysis of Cd uptake, uptake factor (UF), enrichment factor (EF) and translocation factor (TF) patterns revealed that Cd hyperaccumulating properties of R. globosa are based on the high water/nutrients demand and strong tolerance to Cd, although weak protection against Cd uptake by root system was also observed. Amendment with GSH enhanced Cd availability to plant and its uptake from soil, but exerted no effect on Cd translocation in plants. In the light of the results, the use of R. globosa for phytoremediation of moderately polluted agricultural lands as forecrop or aftercrop, and the GSH-assisted phytoremediation of highly polluted post-industrial sites seem to be viable options.

Sulfur application modifies cadmium availability and transfer in the soil-rice system under unstable pe+pH conditions

The objective of this study was to investigate the responses of cadmium (Cd) availability and transfer in the soil-rice system to added sulfur (S) under unstable pe + pH conditions. Different water management conditions (flooding and aerobic treatments) cause changes in the soil pe + pH. We conducted a pot experiment to investigate the influence of S supply on soil Cd availability and Cd accumulation in rice plants (Oryza sativa L.), using three water regimes (continuous dryness, alternating dry-wet for one cycle, and continuous flooding) combined with two S concentrations (0 and 300 mg/kg). The results showed that the flooding treatment was more effective in decreasing soil pe + pH, Cd availability, and Cd accumulation in rice tissues than were the aerobic treatments. S-induced reduction in Cd uptake and translocation in rice was attributed to the decreased soil pe + pH values and enhanced biosynthesis of phytochelatins (PCs) and glutathione (GSH) in rice roots. Microscopic examination showed that the flooding treatment with added soil S resulted in better rice root growth. Element dispersive spectrometer (EDS) analysis indicated that S addition and flooding treatment promoted the formation of iron plaques and increases in Fe concentration in rice tissues. Conversely, partial disintegration of the root epidermis was observed in the dry treatment without added S.

Cd uptake by Phytolacca americana L. promoted by cornstalk biochar amendments in Cd-contaminated soil

Cadmium (Cd) contamination is the most extensive pollution in China farmland. A greenhouse pot trial was conducted to investigate the effects of cornstalk biochar on Cd accumulation by Phytolacca americana L. (pokeweed) in Cd-contaminated soil. The Cd concentration increased in leaves, shoots, and roots of plants amended with 5% biochar by 79%, 113%, and 32%, respectively, compared with the pokeweed without biochar. The Cd availability, soil Cd speciation, soil fertility, root biomass, and Cd chemical forms in root were investigated to explore the mechanism by which Cd uptake increased in presence of biochar. The extractability of Cd by DTPA decreased in presence of biochar by 30% compared with that of soil without biochar. The increases occurred with dose of biochar increased in available phosphorus, labile organic carbon, and C/N atom ratio. Although, the dry weight of the aboveground part of the pokeweed decreased by 38.5%, however, the weight of roots increased by 20.8%. Root biomass and microbial activity reached maximum in the treatment that recieved 5% biochar. Cd forms extracted by NaCl and acetic acid (HAc) were predominant in root. When 5% biochar applied to soil, HAc-extracted Cd took up maximum of the increase in root.

Elucidation of the mechanisms into effects of organic acids on soil fertility, cadmium speciation and ecotoxicity in contaminated soil

The remediation effect of organic acids in heavy metal contaminated soil was widely studied. However, the comprehensive evaluation of organic acids on micro-ecological environment in heavy metal contaminated soil was less known. Herein, this experiment was conducted to investigate the impact of malic acid, citric acid and oxalic acid on soil fertility, cadmium (Cd) speciation and ecotoxicity in contaminated soil. Especially, to evaluate the ecotoxicity of Cd, high-throughput sequencing was used to investigate the soil bacterial community structure and diversity after incubation with organic acids. The results showed that obvious changes in soil pH were not observed. Whereas, the contents of available phosphorus (Olsen-P) and alkali hydrolysable nitrogen (Alkeline-N) evidently increased with a significant difference. Furthermore, compared to control, the proportion of acetic acid-extractable Cd increased by 3.06–6.63%, 6.11–9.43% and 1.91–6.22% respectively in the groups amended with malic acid, citric acid and oxalic acid, which indicated that citric acid did better in improving the availability of Cd than malic acid and oxalic acid. In terms of biological properties, citric acid did best in bacteria count increase, enzyme activities and bacterial community structure improvement. Accordingly, these results provided a better understanding for the influence of organic acids on the micro-ecological environment in Cd contaminated soil, based on physicochemical and biological analysis.

Cadmium accumulation, availability, and rice uptake in soils receiving long-term applications of chemical fertilizers and crop straw return.

Fertilization and straw return have been widely adopted to maintain soil fertility and increase crop yields, but their long-term impacts on the accumulation and availability of cadmium (Cd) in paddy soils are still unconfirmed. Therefore, this study was undertaken in central China to investigate the accumulation, availability, and subsequent uptake of Cd by rice (Oryza sativa L.) in two adjacent field trials (P1 and P2, lasting for 10 and 12years, respectively) under long-term straw return or in combination with chemical fertilizers. Obvious Cd accumulation, probably due to the notable Cd input from irrigation and traffic exhaust in the bulk soil (0-20cm) of P1, was observed. The bulk soil of P2 received homogeneous straw return and chemical fertilizers, as did that of P1; however, the P2 soil almost showed Cd balance. Long-term straw return increased the portion of soil DTPA-extractable Cd to the total pool for both sites, but only P1 showed significant differences when compared to the controls. However, the highest Cd concentrations and the maximum bioconcentration factors in rice straw and grain were obtained using solo application of chemical fertilizers at both sites. Continuous additional applications of crop straw, in contrast, resulted in slightly decreased Cd uptake in rice straw, but not in grain. These findings demonstrate that neither long-term straw return nor fertilization leads directly to notable Cd accumulation, but that the promotion effects of long-term chemical fertilizer applications on Cd uptake in rice need more attention.

Potassium affects the phytoextraction potential of Tanzania guinea grass under cadmium stress.

The supply of potassium (K) is a strategy to increase the tolerance of plants exposed to Cd toxicity. The aim of this study was to verify the influence of K on the growth and potential of Tanzania guinea grass (Panicum maximum Jacq. cv. Tanzania (syn. Megathyrsus maximus (Jacq.) B.K. Simon & S.W.L. Jacobs)) for Cd phytoextraction as well as to evaluate nutritional attributes of this grass under conditions of Cd stress. The experiment was conducted in a randomized complete block design, using a 3 × 4 factorial arrangement, with three replications. Three rates of K (0.4, 6.0, and 11.6 mmol L-1) were combined with four rates of Cd (0.0, 0.5, 1.0, and 1.5 mmol L-1) in nutrient solution. Two plant growth periods were evaluated. The increase in K supply to plants exposed to Cd rates of up to 1.0 mmol L-1 caused increase in morphogenic and production attributes, as well as reduction in tiller mortality rate, in the second growth period. K concentrations (in both harvests) increased, while calcium and magnesium concentrations in the second harvest decreased with increasing Cd rates. The high availability of Cd (1.5 mmol L-1) in the nutrient solution caused decrease in relative chlorophyll index (RCI) in both harvests. The high supply of K to plants exposed to Cd resulted in high shoot dry mass production, reducing Cd concentration in the photosynthetic tissues (which means great tolerance of the plant) and increasing the accumulation of this metal in the shoots that can be harvested. Therefore, K increases the Cd phytoextraction capacity of Tanzania guinea grass.

Soil washing remediation of heavy metal from contaminated soil with EDTMP and PAA: Properties, optimization, and risk assessment

Soil washing is a rapid and efficient remediation technique for soil contaminated by heavy metals. In this study, Cd, Pb, and Zn were removed from contaminated soil by ethylenediamine tetra (methylene phosphonic acid) (EDTMP) and polyacrylic acid (PAA). We then investigated the effect of varying the concentration, pH and duration of the washing processes. Single-factor experiments suggest that the PAA washing process may be dominated by electrostatic adsorption, and is suitable for remediation under weak acid and neutral conditions. Meanwhile, EDTMP remediation might be dominated by chelation, which is favorable in strong acid and alkaline environments. In a quadratic saturation D-optimization design (QSDD), we optimized the washing parameters and further explored the washing mechanism including primary factor, principal effect, interaction effect, and the optimal washing conditions, with simultaneously changing multiple influencing factors. The optimum efficiencies of Cd, Pb, and Zn removal were 92.74%, 96.14%, and 50.76% respectively in EDTMP remediation, and 84.62, 79.24, and 41.66% respectively in PAA remediation. The washing processes effectively reduced the availability of Cd, Pb, and Zn in contaminated soil, without noticeably affecting soil chemical properties. Therefore, the washing incurred little ecological risk. EDTMP and PAA are suitable remediation agents of soil contaminated by heavy metals.

Implications of spatial heterogeneity of tailing material and time scale of vegetation growth processes for the design of phytostabilisation.

Phytostabilisation projects for tailing dams depend on processes occurring at spatial scales of 106 m2 and at decadal time scales. Most experiments supporting the design and monitoring of such projects have much smaller spatial and time scales. Usually, they are only designed for one single scale. Here, we report the results of three coupled experiments performed at pot, lysimeter and field plot scales using six sampling periodstimes from 3 to 20 months. The work explicitly accounts for the sampling times when evaluating the effects of amendments on the performance of plants grown in tailing substrates. Two treatments with potentially complementary roles were applied: zeolites to decrease availability of Cd, Cu, Pb and Zn and green fertilizer to increase the availability of nutrients. Zeolites have a positive influence on plant development, especially in the early stages. Analyses of the pooled datasets for all sampling times revealed the possibility of predicting plant physiological variables, such as protein concentrations, pigments and oxidative stress enzyme activities, as a function of the factors extracted by principal component analysis from the metal concentrations in plants, phosphorus concentrations in plants, and sampling times. Two potentially general methodological rules were extracted: account for the spatial geochemical variability of tailings, and cover the broadest possible range of time scales by experiments. The proposed experimental methodology can be of general use for the design of tailing dam remediation technologies with improvements involving the set of measured variables and sampling frequency and by carefully relating the costs to the institutional aspects of tailing dam management.

The responses of a soil bacterial community under saline stress are associated with Cd availability in long-term wastewater-irrigated field soil

Long-term wastewater irrigation impacts soil geochemical properties (salinity, pH and soil aggregates) and promotes the bioaccumulation of heavy metals to plants, which may change soil function, decrease soil health, decrease the productivity of farmland, and even cause land degradation. In this study, we explored the impact of saline stress on soil bacterial communities and Cd availability in long-term wastewater-irrigated field soil. Different amounts of saline stress by adding 0.1, 0.3 and 0.5% salts in a fixed proportion (NaCl: Na2SO4: NaHCO3: Na2CO3 = 1:9:9:1) were applied in a 1-year, multiple cropping system with winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) in wastewater-polluted soil. Increased salinity in the soil increased Cd availability: compared with CK (no extra salts addition), addition of 0.5% mixed salts significantly increased soil available Cd up by 67.5% and grain Cd concentration by 43.7%, and adding 0.5% salts also resulted in the increases of soil pH (∼0.5 unit) and electric conductivity (97.4%). Soil saline stress significantly changed major soil microbes in Cd-contaminated soil. Increased saline stress enriched taxa in the Bacillaceae, Staphylococcaceae and Pseudomonadaceae bacterial families, while one family within Proteobacteria (Sphingomonadaceae) was the most sensitive biomarker, based on Cd contamination without saline stress in CK-treated soils. Structural equation modeling (SEM) analysis revealed that soil saline stress induced an increase in soil Cd availability that was regulated by the bacterial community.