Enamel Area Research Articles

Evaluation of the effect of self-assembling peptide and fluoride varnish, alone or in combination with laser irradiation, on artificial enamel caries: a SEM/EDS and Micro-CT study.

The aim of this study was to investigate the effect of remineralization agents such as fluoride varnish and P11-4, alone and in combination with Er: YAG laser, on in-vitro hard tissue repair in artificial enamel lesions. A total of sixty enamel surfaces of 4 × 5mm in size were created on both the buccal and lingual sides of thirty extracted wisdom teeth. Remineralization agents were applied to the specimens that were grouped as follows: Group 1, control; Group 2, fluoride varnish (FV); Group 3, P11-4; Group 4, laser; Group 5, laser + FV; and Group 6, laser + P11-4. The fluorescence level was determined with DiagnoDent. The enamel mineral density, area and volume, and caries lesion area and volume were determined with micro-computed tomography (µCT), surface features were evaluated using scanning electron microscopy (SEM), and elemental analysis was performed using energy dispersive x-ray spectroscopy (EDS) . For specimens treated only with self-assembling peptide P11-4, the caries lesion area (mm2) values were 38.19 and 21.62, and the caries lesion volume (mm3) values were 6.27 and 2.99, respectively for pre- and post-treatment. In combination usage of self-assembling peptide P11-4 and laser, the caries lesion area (mm2) values were 38.39 and 16.91, and the caries lesion volume (mm3) values were 11.15 and 3.64, respectively for pre- and post-treatment. In the application of the P11-4 alone and in combination with laser, there was a statistically significant decrease in DiagnoDent values, an increase in enamel volume(mm3),enamel area(mm2) and mineral density(g/cm3) values and a decrease in caries lesion volume(mm3) and area(mm2) obtained by µCT, and an increase in %Ca and %F values obtained by SEM/EDS analysis (p < 0.05). It was discovered that the samples treated with P11-4 had a considerably higher rise in the Ca/P ratio than the samples treated with FV (p < 0.05). The calcium content increased significantly more when P11-4 application was combined with laser irradiation (p < 0.05). The combined use of self-assembling peptide P11-4 and laser accelerated the remineralization process and increased the remineralization capacity. FV and P11-4, alone or in combination with laser, can be successfully used as remineralization agents in initial enamel caries.

Early onset of enamel formation in Baka pygmy’s deciduous canines

ObjectiveOur aim was to evaluate by enamel microstructure analysis two hypotheses that would explain the early dental eruption in the Bakaparticularity, a shorter crown formation time and/or earlier onset of crown formation. DesignDeciduous canines corresponds to the best teeth to perform the analysis of enamel microstructure. Longitudinal ground sections of 21 deciduous canines from 12 individuals were studied with transmitted light microscopy. Cross-striations, striaes of Retzius (SR) and the neonatal line (NNL) enable to establish the prenatal crown formation time (preCFT), the postnatal crown formation time (postCFT), the crown formation time (CFT) as well as the daily secretion rate (DSR) and the enamel extension rate (EER) and their variation along crown formation. ResultsThe DSR and the EER in the Baka are similar than in other populations with an average DSR of 3.26 µm and EER of 18.18 µm. The preCFT was 154 days, the postCFT 265 days and CFT 419 days. Comparison with other population does not show difference in CFT. However, the preCFT and the postCFT differ, the first is higher and the second lower in the Baka than in other populations. Furthermore, the number of prenatal areas of enamel was greater in the Baka. ConclusionOur analysis suggests that the Baka does not distinguish by a different CFT but the onset of crown formation is earlier than in other groups. Therefore, the early dental eruption in the Baka results from an earlier onset of crown formation.

Remineralization potential of dentifrices with calcium sodium phosphosilicate and functionalized tri-calcium phosphate in the deeper incipient carious lesions: An in vitro study.

This study evaluated the remineralization potential of calcium sodium phosphosilicate and functionalized tri-calcium phosphate (f-TCP) dentifrices in deeper incipient carious lesions (ICLs). Artificial ICLs were created by placing premolars into demineralizing solutions. Teeth were randomly assigned into four groups: calcium sodium phosphosilicate (Group 1), f-TCP (Group 2), 1450 ppm fluoride (Group 3), and distilled water (Group 4), which were subjected to 10-daypH cycling. Mineral density (MD) was assessed using microcomputed tomography (Micro-CT), while hardness (H) and elastic modulus (EM) were assessed using nanomechanical testing. MD % gain was higher in Groups 1-3 than in Group 4. In addition, Groups 1 and 2 exhibited significantly higher MD % gain than Group 3. Also, Groups 1-3 showed significantly higher EM and H values than Group 4 in the outer enamel area; yet, Groups 1 and 2 displayed significantly higher EM and H values than Groups 3 and 4 in the inner enamel. The MD, EM, and H of ICLs significantly increased with the addition of calcium sodium phosphosilicate or f-TCP to fluoridated dentifrices compared to standard fluoride dentifrices. The added active ingredients remineralized the deeper parts of the ICLs, while remineralization at the lesion surface was similar between tested dentifrices.

In vitro assessment of dental erosion caused by clear aligners

The primary objective of this in vitro study was to investigate the erosive potential of enamel under the use of clear aligners (CA), by simulating in vivo conditions experienced by patients who do not remove their CA during the consumption of acidic beverages. In addition, the difference in erosion protection conferred by artificial and human saliva was also evaluated. Sound-extracted human premolars (n = 20) had half of their surfaces protected with acid-resistant nail polish and were randomly distributed into two experimental groups (n = 10): teeth immersed in human saliva or artificial saliva. All teeth had half of their lingual surfaces enclosed by a CA device. The erosive challenges consisted of individual immersion of each sample in citrus acid three times a day, intermediated by immersion in human saliva or artificial saliva for 2 h, during ten days of the erosive protocol. The enamel mineral content was analyzed by high-resolution microtomography. The differential mineral concentration profiles were obtained by subtracting the profile of the mineral concentration of the exposed area and enamel under the CA area from the respective sound area (control). In addition, enamel wear and enamel volume loss were measured. Scanning electron microscopy (SEM) was also performed to analyze the enamel surface. Data were analyzed by two-way ANOVA, followed by the Student-Newman-Keuls test. The enamel wear was higher in teeth immersed in artificial saliva, when compared to human saliva (p < 0.001). The volume loss of the exposed enamel area was lower for tooth immersed in human saliva than in artificial saliva (p < 0.001), during the acid challenge protocol. The use of CA during acid challenges promoted wear and mineral loss of dental enamel, being these changes more pronounced on the enamel surface under the CA. These results open a new path for the development of further studies adopting clinical protocols that promote more accurate responses in the clinical practice during orthodontic treatment.

Relating metric crown dimensions to underlying internal daily secretion rates in antimeric premolars

ObjectivesThe underlying cause for metric differences in antimeric tooth pairs is an important question for understanding dental variation. We hypothesize that localized variation in crown dimensions will be reflected in localized variation in daily enamel secretion rate. DesignCasts of pairs of human premolars from a tissue bank were 3D scanned using an optical scanning system (n = 32). Histological slides were created, and daily secretion rates (DSRs) were recorded in two areas of enamel that corresponded to scanner measurements. Antimeres were compared for both metric measurements and DSRs. Outliers for scanner measures were compared to significant differences in DSRs measurements in right and left teeth in corresponding areas. ResultsThirteen of the 16 individuals differed significantly in antimeric metric measurements, but only ten of those also differed in the underlying DSR. Fifteen of the 16 total individuals differed significantly in at least one DSR area. DiscussionWhile some individuals were outliers for metric measurements and had multiple areas of DSR differences, the majority of individuals had antimeric DSR differences regardless of metric differences. While there was no conclusive correlation between 3D metric analysis and underlying DSR differences, the most important result of this study is that DSR differences between antimeres are common.

CAD/CAM leucite-reinforced glass-ceramic for simulation of attrition in human enamel in vitro

ObjectiveInvestigate attrition simulation using CAD/CAM leucite-reinforced glass-ceramic antagonists on occlusal vs. buccal enamel. MethodsThree dental materials with known wear rates (resin-modified glass-ionomer, micro-filled, and fine particle composites) validated the wear simulator (CAD/CAM glass-ceramic antagonists, 200 cycles, 80 N load, deionised water irrigation, 0.7 mm sliding movement). Following this, human molars were sectioned into paired occlusal and buccal polished samples (n = 8/gp). Exposed 1.5 mm Ø enamel areas were subjected to attritional wear with and without pre-immersion in citric acid (5 min, 0.3%, pH 3.8). Profilometry measured step-height enamel wear and surface microhardness at different depths was calculated using Vickers indentation at 0.1 N and 0.5 N loads. ResultsDental material wear using the CAD/CAM antagonists showed consistency with previous data: mean (SD) resin-modified glass ionomer material loss of 177.77 (16.89) µm vs. 22.15 (1.30) µm fine particle hybrid composite resin wear vs. 13.63 (1.02) µm micro filled composite resin wear (P < 0.001). The coefficient of variation was less than 10%. Following validation, enamel sample wear was significantly increased when attrition was introduced (P < 0.001) independent of buccal vs. occlusal sample location (P < 0.05). Attrition resulted in occlusal wear of 26.1 ± 4.5 µm vs. buccal 26.3 ± 1.2 µm and attrition/erosion resulted in occlusal wear of 26.05 ± 4.46 µm vs. buccal 25.27 ± 1.16 µm. Whereas erosion-alone resulted in occlusal wear of 1.65 ± 0.13 µm and buccal 1.75 ± 0.03 µm. Microhardness testing at different loads revealed significantly greater hardness reductions in occlusal enamel vs. buccal enamel for 0.1 KgF indentations (P < 0.001) whereas in contrast 0.5 KgF indentations showed no differences. SignificanceWear simulation with CAD/CAM glass ceramic antagonists produced consistent wear in dental materials and human enamel, regardless of enamel surface origin. Lighter (0.1 KgF) hardness testing of occlusal vs. buccal origin revealed damage to the mechanical integrity of the superficial worn enamel.

Enhancing Precision in L-band Electron Paramagnetic Resonance Tooth Dosimetry: Incorporating Digital Image Processing and Radiation Therapy Plans for Geometric Correction.

Following unforeseen exposure to radiation, quick dose determination is essential to prioritize potential patients that require immediate medical care. L-band electron paramagnetic resonance tooth dosimetry can be efficiently used for rapid triage as this poses no harm to the human incisor, although geometric variations among human teeth may hinder accurate dose estimation. Consequently, we propose a practical geometric correction method using a mobile phone camera. Donated human incisors were irradiated with calibrated 6-MV photon beam irradiation, and dose-response curves were developed by irradiation with a predetermined dose using custom-made poly(methyl methacrylate) slab phantoms. Three radiation treatment plans for incisors were selected and altered to suit the head phantom. The mean doses on tooth structures were calculated using a commercial treatment planning system, and the electron paramagnetic resonance signals of the incisors were measured. The enamel area was computed from camera-acquired tooth images. The relative standard uncertainty was rigorously estimated both with and without geometric correction. The effects on the electron paramagnetic resonance signal caused by axial and rotational movements of tooth samples were evaluated through finite element analysis. The mean absolute deviations of mean doses both with and without geometric correction showed marginal improvement. The average relative differences without and with geometric correction significantly decreased from 21.0% to 16.8% (p = 0.01). The geometric correction method shows potential in improving dose precision measurement with minimal delay. Furthermore, our findings demonstrated the viability of using treatment planning system doses in dose estimation for L-band electron paramagnetic resonance tooth dosimetry.

Effect of an experimental TiF4/NaF solution in preventing tooth erosion

ObjectiveThis was a randomized, double-blind, parallel, placebo-controlled in vivo study investigating the protective potential of a titanium tetrafluoride/ sodium fluoride (TiF4/NaF) solution compared to its respective positive and negative controls under an in vivo model, as well as the perception of participants regarding the use of this experimental solution. MethodsAfter the ethics approval and the selection procedures, 33 participants were divided into three treatments: TiF4/NaF solution (500 ppm F, pH 4.4); AmF/NaF/SnCl2-mouthwash (500 ppm F, pH 4.5) and water (pH 7.0) (n = 11). After professional cleaning, the participants rinsed with one of the solutions for one minute and waited two hours for the erosive challenge. The erosive solution (1 % citric acid, pH 2.5) was applied for 10 s on each central incisor (enamel area: 4 mm2) and collected for calcium analysis using III Arsenazo colorimetric method. The Ca2+ release data were compared using Kruskal-Wallis/ Dunn tests (p < 0.05). ResultsTeeth treated with both fluoride solutions released less calcium into the acid (median and interquartile interval: TiF4/NaF – 0.45/0.19 mM and AmF/NaF/SnCl2 – 0.46/0.15 mM Ca2+, p = 0.99) compared to the negative control (1.12/0.42 mM Ca2+, 60 % reduction, p < 0.0006). For both F solutions, only one participant per group reported unpleasant taste. Four participants belonging to AmF/NaF/SnCl2-mouthwash reported burning sensation post-rinse, while only one participant described such feeling after TiF4/NaF rinsing. ConclusionThe experimental TiF4/NaF solution was as effective as the commercial AmF/NaF/SnCl2-mouthwash in protecting enamel against erosive demineralization with a good acceptability by the participants.

Variation in enamel and dentine mineral concentration and density in primate molars

ObjectiveVariation in enamel and dentine mineral concentration and total effective density can be reliably collected using Micro-CT scans. Both variables are suggested to reflect mechanical properties such as hardness and elastic modulus in dental tissues, meaning Micro-CT methods allow relative composition and mechanical properties to be collected non-destructively. Design16 lower molars from 16 Catarrhine primates were Micro-CT scanned alongside hydroxyapatite phantoms using standardized settings and methods to calculate mineral concentration and total effective density. Mineral concentration, total effective density and thickness of dentine and enamel were calculated for four cusps, representing each 'corner' of the tooth and four lateral crown positions (i.e., mesial, buccal, lingual and distal). ResultsThe results show mean mineral concentration and total effective density values were higher in areas of thicker enamel, while the opposite was observed for dentine. Buccal positions had significantly higher mineral concentration and total effective density values than lingual areas. Cuspal positions had higher mean values than lateral enamel, for both dentine (mineral concentration cuspal: 1.26 g/cm3; lateral: 1.20 g/cm3) and enamel (mineral concentration cuspal: 2.31 g/cm3; lateral: 2.25 g/cm3). Mesial enamel had significantly lower values than other locations. ConclusionsThese common patterns across Catarrhine taxa may be linked to functional adaptations related to optimization of mastication and tooth protection. Variation in mineral concentration and total effective density may also be associated with wear and fracture patterns, and can be used as baseline information to investigate the effect of diet, pathological changes and aging on teeth through time.

The Indicators of Molar Coefficients of the Chemical Elements in Dentin and their Correlation with Enamel

Objectives:Determination of molar coefficients of the chemical elements in dentin, their possible correlation with tooth enamel. Material and Methods:There were examined 29 clinically extracted teeth of both jaws and their longitudinal sections (12 clinically intact teeth, 10 teeth with wedge-shaped defects, 7 teeth with cervical caries) of the patients aged 25to 54 with the help of JSM-6490 LV focused beam electron microscope (scanning) with system of energy-dispersive X-ray microanalysis. We have determined the chemical composition of 290 enamel areas and 235 dentine areas in the incisal region (tubercle), equator, cervical area as a percentage of the weight amounts of carbon, oxygen, calcium, phosphorus, sodium, magnesium, sulfur, chlorine, zinc, potassium, aluminum, we have identified their molar coefficients. Results: The chemical composition of dentin differed in the values of Na/Mg, Al/Zn, Mg/Ca, K/Na (p≤0.05) in all studied topographical regions of the samples. Higher values of Na/Mg and lower values of Al/Zn were identified in the teeth with cervical caries (p≤0.05). Correlation was found in dentin: inverse between Ca/P and P/Ca, Mg/Ca and Na/Mg, Ca/Mg and Mg/Ca, Mg/P and Na/Mg, Mg/P and Ca/Mg, direct between Ca/Mg and Na/Mg, Mg/P and Mg/Ca (p≤0.05). High correlation was determined between molar coefficients in enamel and dentin: Mg/Ca and Mg/P (direct), Ca/P and P/Ca (inverse), p&amp;lt;0.0001. Conclusions:The differences in the indicators of molar coefficients in dentin, their correlation with enamel are probably associated with the peculiarities of the pathological processes in the cervical region and it requires further study.

Evaluation of sexual dimorphism in human premolars using longitudinal ground sections- A cross sectional study

Introduction: Sex determination plays an important role in forensics. On average, males possess larger tooth crowns than females in contemporary human populations, although the degree of dimorphism varies within different populations. Ground sectioning of teeth can provide an accurate measurement of enamel and dentinal thickness and can be used for gender determination in forensic analysis.&#x0D; Aim: The main aim of this study is to evaluate sexual dimorphism in human premolars using longitudinal ground sections.&#x0D; Materials &amp; Method: In this study extracted premolar teeth of males and females were taken. Longitudinal ground sectioning of these teeth was made and later examined under light microscope under customized grids.&#x0D; Results: Data was statistically analyzed and results were tabulated. It was found that enamel area and average enamel thickness was higher in females than males and coronal dentinal areas were more in males.&#x0D; Conclusion: Results of this study suggest that the differences in crown morphology among males and females can be used as a potential tool in forensic analysis.

Anticaries potential of a fluoride foam.

Foam has been used worldwide as a vehicle for the professional application of fluoride and hypothetically should have the same anticaries potential as conventional fluoride gel (F-gel) in terms of the formation of reaction products with enamel. Thus, the ability of Flúor Care® foam (FGM, Joinville, SC, Brazil, 12,300 ppm F, acidulated) to react with enamel was evaluated in comparison with Flúor gel® (DFL, Rio de Janeiro, RJ, Brazil, 12,300 ppm F, acidulated). Slabs (n=10/group) of sound enamel and with caries lesion were used, in which the concentrations of total fluoride (TF), and loosely (CaF2-like) and firmly (FAp) bound types were determined. The importance of agitation during application was previously tested. The determinations were made with fluoride ion-specific electrode and the results were expressed in μg F/cm² of the treated enamel area. ANOVA and Tukey tests were used to analyze the difference among treatments, independently for sound and carious enamel. The agitation of the products during application significantly increased the reactivity of the foam (p<0.05), but not that of the gel (p>0.05). The foam did not differ from F-gel (p>0.05) concerning the formation of TF and CaF2-like in sound or carious enamel. Regarding FAp, the foam did not differ from F-gel (p>0.05) in the carious enamel, but the concentration in the sound was lower (p<0.05). The results show that this commercial fluoride foam tested needs to be agitated during application to improve its reactivity with enamel, which raises a question about other brands.

Three-dimensional microscopic comparison of wear behavior between immature and mature enamel: an in vitro study

BackgroundDental enamel, the hardest outermost layer of a human tooth, is subjected to occlusal forces throughout life during different oral function as talking, mastication etc. Due to this continuous stress, wear causes the loss of this protective shell. This study aimed to detect microscopic differences in enamel’s wear behavior among different age groups of adolescents and adults.Aims and methodsEnamel specimens from immature open-apex and mature closed-apex premolars were subjected to simulated occlusal wear of impact and sliding wear test ISWT. Upper and lower enamel specimens were made to come in contact under controlled conditions. The enamel specimens’ surfaces were examined using different microscopes. The upper and lower specimens were subjected to the following tests; pre-test light microscopy examination, enamel specimens’ preparation for ISWT, scanning laser confocal microscopy of upper specimens, three-dimensional (3D) colored laser microscope and a Profilometer imaging of the lower specimens.ResultsWear characteristics, including wear areas, crater depths, and relation to enamel microstructures, differed among different age groups. Immature enamel from the upper specimens was more resistant to chipping than mature enamel with no statistically significant wear area difference. The immature enamel craters from the lower specimens were wider and deeper than those in the mature enamel; the wear areas in the mature enamel in the lower specimens were almost flat and smooth. The wear areas in the immature enamel in the lower specimens were significantly larger than those in the mature enamel.ConclusionsWear characteristics of the immature enamel are different from those of the mature enamel. Hence, it should be repaired using restorative materials with compatible wear properties.

Effect of Temporary Cement, Surface Pretreatment and Tooth Area on the Bond Strength of Adhesively Cemented Ceramic Overlays—An In Vitro Study

Several viewpoints have been reported regarding the effect of temporary cements, different surface pretreatment protocols before adhesive cementation, and predictive factors. This in vitro study tested if temporary cement, pretreatment of the tooth surface, the size of enamel or dentine influence adhesive cementation to zirconia ceramics. Twenty premolars were prepared for determination of enamel and dentin area, bond strength test and failure analysis. The samples were divided into two groups: untreated prior adhesive cementation (n = 10) and with temporary cementation done, pretreated prior adhesive cementation (n = 10). Zirconia overlays (Katana Zirconia STML) were cemented on the grounded flat teeth surfaces using Panavia V5. An additional six premolars underwent dentine tubule analysis with SEM to detect temporary cement residues after temporary cementation on an untreated tooth surface (n = 3) and on a pretreated surface (n = 3). The independent sample t-test was used to compare the two groups and the means of the total tooth, dentin or enamel areas did not differ significantly between the untreated and pretreated specimens. The mean tensile bond strength was significantly (p = 0.005) higher in the pretreated specimens (337N) than in the untreated ones (204N). The overall multivariable linear regression model with three predictors (surface pre-treatment, enamel area and dentine area) was significant (p = 0.003), among which the size of enamel was the strongest predictor (β = 0.506; p = 0.049), followed by the pretreatment effect (β = 0.478; p = 0.001) and the size of dentin area (β = -0.105; p = 0.022).

Hardness and indentation modulus of human enamel and dentin

Information on mechanical properties of human enamel and dentin and effect of load on their deformation and damage tolerance is very important in the development of successful dental materials. In this study, the hardness, indentation modulus and deformation characteristics of tooth have been investigated across the lingual–buccal enamel and dentin cross‐section by instrumented indentation up to 1500‐nm indentation depth. We realized and examined the distribution of hardness and modulus at different areas of enamel and dentin with the aim to understand deformation behaviour at these different locations. Scanning electron microscopy was used for the microstructure characterization of the enamel and dentin and their deformation behaviour. Raman spectroscopy analyses were carried out for determination of the enamel and dentin composition as well. The average values of hardness and modulus for enamel in the occlusal area are 5.35 ± 0.19 and 98.1 ± 1.5 GPa, in the inner area 3.68 ± 0.5 and 81.8 ± 5 GPa and in the area close to the dentin–enamel junction 2.83 ± 0.27 and 71.4 ± 4.1 GPa, respectively. The average values of hardness and modulus of dentin in the area close to the dentin–enamel junction are 0.71 ± 0.05 and 21.27 ± 0.76 GPa and in the inner area 0.65 ± 0.06 and 19.79 ± 0.93 GPa, respectively. The effect of the indentation location on deformation and mechanical properties was clarified.

Evaluation of the Efficacy of CPP-ACP Remineralizing Mousse in MIH White and Yellow Opacities-In Vitro Vickers Microhardness Analysis.

Remineralization of tooth enamel can be partially achieved by the application of a casein phosphopeptides and amorphous phosphate (CPP-ACP) complex. However, evidence to support its effectiveness in Molar-incisor-hypomineralization (MIH)-affected teeth is scarce. The study’s aim is to evaluate the efficacy of CPP-ACP mousse in remineralizing MIH-affected enamel using a Vickers microhardness test. Two groups of enamel opacities of hypomineralized permanent teeth, white (group A) and yellow (group B) lesions (n = 14), went through a 28-day treatment protocol with GC Tooth Mousse. Before and after treatment, microhardness was measured in three different areas of each tooth (hypomineralized, transition, and outside the hypomineralized area). Data were analyzed using parametric and non-parametric tests with a significance of p < 0.05. The mean microhardness values increased in the hypomineralized and transition areas in both groups after the treatment protocol (in group A, 105.38 ± 11.70 to 158.26 ± 37.34; 123.04 ± 22.84 to 156.33 ± 35.70; in group B, 108.63 ± 14.66 to 143.06 ± 22.81; 132.55 ± 20.66 to 146.00 ± 12.88) and the differences pre/post-treatment were statistically significant within each group (p < 0.001 for both groups). Between groups, there was a statistically significant difference in the same areas (hypomineralized: p = 0.003; transition: p = 0.008) with a higher improvement in enamel hardness in group A. Topical application of CPP-ACP showed an increase in the physical strength of the hypomineralized and transition areas of MIH-affected enamel, likely due to an increase in mineral content.

The synergetic effect of pulp chamber extension depth and occlusal thickness on stress distribution of molar endocrowns: a 3-dimensional finite element analysis

The aim of this study was to evaluate the effects of butt margin, occlusal thickness and pulp chamber extension depth on stress distributions on mandibular molar endodontically treated teeth (ETT) with EMAX endocrown restoration using 3-dimensional finite element analysis (FEA). The FEA models of endocrown with flat surface or curve surface of butt margin were firstly evaluated stress distributions, and then 9 FEA models of endocrown with 1-, 2- or 3-mm pulp chamber extension depth and 1-, 2- or 3-mm occlusal thickness were generated using curve surface of butt margin. In all of FEA models, a 200 N of vertical load or horizontal load was applied, and the von Mises stress (VMS) were evaluated. The results showed that curve surface of butt margin offered more adhesive area of enamel, though VMS on the prepared teeth was similar in flat surface and curve surface models. In 9 endocrown models, 2-mm occlusal thickness showed the lowest VMS on restorations, teeth tissue and root furcations, and 2-mm extension depth displayed the lowest VMS on root furcations under vertical load. Also, 2-mm extension depth exhibited the lowest VMS on restorations and teeth tissue under horizontal load. Within the limitations of this FEA study, the results of this study could be used as an aid for dentists to better devise endocrown restorations.Graphical abstract

PECULIARITIES OF CHEMICAL INTRAOPERATIVE TREATMENT OF AUTOGRAFTS FROM DENTIN DURING ALVEOLAR BONE PLASTY

The aim of the study was to assess the possibility of intraoperative decalcification of autografts from dentin using a solution of EDTA salts (ethylenediaminetetraacetic acid) 17% at an exposure of up to 10 minutes during alveolar bone plasty. Materials and Methods: 19 patients aged 20 to 45 years, according to indications, 24 third molars were routinely removed, from which 48 plates 1.5-2 mm thick were made. The obtained samples were divided into 4 groups of 12 plates in each group. The samples of the first group were not treated with 17% EDTA salt solution, the samples of the 2nd group were treated for 1 minute, the 3rd group - 5 minutes, the 4th group - 10 minutes. After processing, all samples were washed under distilled water for 1 minute. Further, using a scanning electron microscope "FEI Quanta 250" (USA) in the area of​​enamel, dentin and dentin at the border with the pulp chamber of the prepared samples, the microstructure was investigated, and the qualitative and quantitative chemical composition of dental tissues was also studied. Results: The amount of Ca in the studied samples of the 1st group: in the enamel area was - 42.32% ± 1.1; dentine – 42.25% ± 1.3; dentin at the border with the pulp chamber - 29.71% ± 1.5; group 2: in the enamel area - 48.95% ± 0.9.1; dentin – 37.39% ± 1.2; dentin at the border with the pulp chamber - 19.32% ± 1.1; group 3: in the enamel area - 39.3% ± 1.9; dentine - 38.3% ± 1.4; dentin at the border with the pulp chamber - 31.06% ± 1.1; 4th group: in the enamel area - 37.85% ± 0.8; dentine - 37.8% ± 1.5; dentine at the border with the pulp chamber - 26.6% ± 1.2 (p&lt;0.05). Conclusion: Thus, the treatment with 17% EDTA salt solution for up to 10 minutes exposure of dentin autografts used in the form of plates does not significantly affect their microstructure, qualitative and quantitative composition (CI = 95%), and the degree of demineralization. Hence, it is impractical to carry out this manipulation intraoperatively in alveolar bone plasty.

Enamel growth rate variation of inner, mid, and outer enamel regions between select permanent tooth types across five temporally distinct British samples

ObjectiveThis study explored differences in the regional daily growth rates of human enamel between tooth types across a temporal transect in Britain. MethodsUpper permanent central incisors (n = 81), upper permanent canines (n = 69), and upper and lower permanent first molars (n = 115) from Roman, Pre-Medieval, Medieval, and Modern day populations were analysed using histological methods. Daily secretion rates (DSRs) were collected for inner, mid, and outer regions of cuspal and lateral enamel for each tooth type and temporal sample. Variation in DSRs between the tooth types, within each population, was sought using Welch’s tests. ResultsNumerous significant differences were observed in DSRs between equivalent enamel regions of different tooth types. The majority of differences were observed between molars and the anterior teeth, but there were no obvious trends as to which typically grew faster/slower, nor was there any consistency across the temporal samples. In contrast, comparisons between incisors and canines yielded minimal differences and variation, when significant, was found to be enamel area- and sample-specific. ConclusionsThis study presents evidence for a high level of variation in DSRs between anterior teeth and first molars of the permanent dentition. This variation appears sporadic with no clear trend outside of anterior tooth comparisons, where analyses of the Late Pre-Medieval and Modern-day populations highlight how DSRs within cuspal and lateral enamel can vary independently.

Monitoring silver diamine fluoride application with optical coherence tomography and thermal imaging: An in vitro proof of concept study.

ObjectivesThe purpose of this study was to show that optical coherence tomography (OCT) and thermal imaging can be used to monitor changes in the structure and activity of caries lesions over time after treatment with silver diamine fluoride (SDF).MethodsArtificial caries lesions were formed on enamel and dentin bovine blocks. Each block was partitioned into five windows with the central three windows exposed to a demineralization solution to create lesions: one sound window served as a sound control (SC), one sound window was exposed to SDF to serve as a test control (SCT), one lesion window served as a lesion control (LC), one lesion window received one application of SDF (L1), while the other lesion window received two applications of SDF (L2). Each window was scanned using OCT before SDF application, and every week subsequently, for 12 weeks after initial SDF treatment. Changes in the mean intensity and the width of the peak of increased reflectivity due to the lesion and SDF along with the intensity at a depth of 180 µm from the surface representing optical penetration through the lesion were monitored. Changes in the heat lost, ΔQ (temperature integrated over time) of each window during drying with air were also monitored using a thermal imaging camera. Transverse microradiography (TMR), and high‐resolution microscopy were also used for the analysis of selected samples.ResultsThe reflectivity and optical penetration of sound and lesion areas of enamel and dentin manifested significant changes in OCT images after SDF application. Thermal imaging showed significant differences in ΔQ indicative of permeability changes in the sound and lesion areas of enamel and dentin after SDF application.