Abstract
The aim of the study was to assess the possibility of intraoperative decalcification of autografts from dentin using a solution of EDTA salts (ethylenediaminetetraacetic acid) 17% at an exposure of up to 10 minutes during alveolar bone plasty. Materials and Methods: 19 patients aged 20 to 45 years, according to indications, 24 third molars were routinely removed, from which 48 plates 1.5-2 mm thick were made. The obtained samples were divided into 4 groups of 12 plates in each group. The samples of the first group were not treated with 17% EDTA salt solution, the samples of the 2nd group were treated for 1 minute, the 3rd group - 5 minutes, the 4th group - 10 minutes. After processing, all samples were washed under distilled water for 1 minute. Further, using a scanning electron microscope "FEI Quanta 250" (USA) in the area ofenamel, dentin and dentin at the border with the pulp chamber of the prepared samples, the microstructure was investigated, and the qualitative and quantitative chemical composition of dental tissues was also studied. Results: The amount of Ca in the studied samples of the 1st group: in the enamel area was - 42.32% ± 1.1; dentine – 42.25% ± 1.3; dentin at the border with the pulp chamber - 29.71% ± 1.5; group 2: in the enamel area - 48.95% ± 0.9.1; dentin – 37.39% ± 1.2; dentin at the border with the pulp chamber - 19.32% ± 1.1; group 3: in the enamel area - 39.3% ± 1.9; dentine - 38.3% ± 1.4; dentin at the border with the pulp chamber - 31.06% ± 1.1; 4th group: in the enamel area - 37.85% ± 0.8; dentine - 37.8% ± 1.5; dentine at the border with the pulp chamber - 26.6% ± 1.2 (p<0.05). Conclusion: Thus, the treatment with 17% EDTA salt solution for up to 10 minutes exposure of dentin autografts used in the form of plates does not significantly affect their microstructure, qualitative and quantitative composition (CI = 95%), and the degree of demineralization. Hence, it is impractical to carry out this manipulation intraoperatively in alveolar bone plasty.
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