Aqueous Nitrate Research Articles

Sustainable Electrosynthesis of Cyclohexanone Oxime through Nitrate Reduction on a Zn-Cu Alloy Catalyst.

Cyclohexanone oxime is an important precursor for Nylon-6 and is typically synthesized via the nucleophilic addition-elimination of hydroxylamine with cyclohexanone. Current technologies for hydroxylamine production are, however, not environment-friendly due to the requirement of harsh reaction conditions. Here, we report an electrochemical method for the one-pot synthesis of cyclohexanone oxime under ambient conditions with aqueous nitrate as the nitrogen source. A series of Zn-Cu alloy catalysts are developed to drive the electrochemical reduction of nitrate, where the hydroxylamine intermediate formed in the electroreduction process can undergo a chemical reaction with the cyclohexanone present in the electrolyte to produce the corresponding oxime. The best performance is achieved on a Zn93Cu7 electrocatalyst with a 97% yield and a 27% Faradaic efficiency for cyclohexanone oxime at 100 mA/cm2. By analyzing the catalytic activities/selectivities of the different Zn-Cu alloys and conducting in-depth mechanistic studies via in situ Raman spectroscopy and theoretical calculations, we demonstrate that the adsorption of nitrogen species plays a central role in catalytic performance. Overall, this work provides an attractive strategy to build the C-N bond in oxime and drive organic synthesis through electrochemical nitrate reduction, while highlighting the importance of controlling surface adsorption for product selectivity in electrosynthesis.

Exploitation of mangliculous marine fungi, Amarenographium solium, for the green synthesis of silver nanoparticles and their activity against multiple drug-resistant bacteria

Abstract The green synthesis pathway for silver nanoparticles (AgNPs) used in bacterial treatment is regarded as crucial because of its cost-effectiveness, nontoxicity, and eco-friendliness. During the present work, the mangliculous marine fungi Amarenographium solium isolated from the Arabian Gulf Coast of Saudi Arabia were utilized for the synthesis of AgNP, through the bio-reduction of aqueous silver nitrate (AgNO3) solution. The success in AgNP synthesis was visually identified by the development of dark brown color in the cell-free filtrate and was further confirmed by ultraviolet–visible spectroscopy, which showed a peak at 425 nm. The AgNPs produced were further characterized using X-ray diffraction data analysis that proved the bioreduction of silver to 20 nm, and transmission electron microscopy revealed the formation of well-dispersed spherical nanoparticles with an average mean size of 12 nm. The optimization reaction parameters of temperature, pH, and metal salt concentration were carried out and resulted in a combination of 30°C, 7 and 1.5 mM, respectively, for rapid and maximum yield production. The antibacterial activity of the produced nanoparticles was evaluated using the two-fold microdilution method and showed a minimum inhibitory concentration of 9.375 μg/mL of AgNP against multiple drug-resistant bacterial strains.

Tuning the Selectivity of Nitrate Reduction via Fine Composition Control of RuPdNP Catalysts.

Herein, aqueous nitrate (NO3 -) reduction is used to explore composition-selectivity relationships of randomly alloyed ruthenium-palladium nanoparticle catalysts to provide insights into the factors affecting selectivity during this and other industrially relevant catalytic reactions. NO3 - reduction proceeds through nitrite (NO2 -) and then nitric oxide (NO), before diverging to form either dinitrogen (N2) or ammonium (NH4 +) as final products, with N2 preferred in potable water treatment but NH4 + preferred for nitrogen recovery. It is shown that the NO3 - and NO starting feedstocks favor NH4 + formation using Ru-rich catalysts, while Pd-rich catalysts favor N2 formation. Conversely, a NO2 - starting feedstock favors NH4 + at ≈50 atomic-% Ru and selectivity decreases with higher Ru content. Mechanistic differences have been probed using density functional theory (DFT). Results show that, for NO3 - and NO feedstocks, the thermodynamics of the competing pathways for N-H and N-N formation lead to preferential NH4 +or N2 production, respectively, while Ru-rich surfaces are susceptible to poisoning by NO2 - feedstock, which displaces H atoms. This leads to a decrease in overall reduction activity and an increase in selectivity toward N2 production. Together, these results demonstrate the importance of tailoring both the reaction pathway thermodynamics and initial reactant binding energies to control overall reaction selectivity.

Bioinspired Tandem Electrode for Selective Electrocatalytic Synthesis of Ammonia from Aqueous Nitrate.

The electrocatalytic nitrate reduction reaction (NO3RR) has recently emerged as a promising technique for readily converting aqueous nitrate (NO3-) pollutants into valuable ammonia (NH3). It is vital to thoroughly understand the mechanism of the reaction to rationally design and construct advanced electrocatalytic systems that can effectively and selectively drive the NO3RR. There are several natural enzymes that incorporate molybdenum (Mo) and that can activate NO3-. Based on this, a cadmium (Cd) single-atom anchored Mo2TiC2Tx electrocatalyst (referred to as CdSA-Mo2TiC2Tx) through the NO3RR to generate NH3 was rationally designed and demonstrated. In an H-type electrolysis cell and at a current density of 42.5 mA cm-2, the electrocatalyst had a Faradaic efficiency of >95% and an impressive NH3 yield rate of 48.5 mg h-1 cm-2. Moreover, the conversion of NO3- to NH3 on the CdSA-Mo2TiC2Tx surface was further revealed by operando attenuated total reflection Fourier-transform infrared spectroscopy and an electrochemical differential mass spectrometer. The electrocatalyst significantly outperformed Mo2TiC2Tx as well as reported state-of-the-art catalysts. Density functional theory calculations revealed that CdSA-Mo2TiC2Tx decreased the ability of the d-p orbital to hybridize with NH3* intermediates, thereby decreasing the activation energy of the potential-determining step. This work not only highlights the application prospects of heavy metal single-atom catalysts in the NO3RR but also provides examples of bio-inspired electrocatalysts for the synthesis of NH3.

Highly active electroreduction of nitrates to ammonia over a zeolitic imidazolium framework-derived Fe single-atom catalyst with sulfur-modified asymmetric active centers

The electroreduction of aqueous nitrate (NO3–) to ammonium is an energy-efficient process that helps protect the environment and facilitates ammonia production. However, a fine optimization of the catalyst structure containing active centers should be performed to improve the efficiencies of NO3– reduction and NH4+ production. Herein, a zeolitic imidazolate framework (ZIF)-derived sulfur-modified Fe single-atom catalyst is developed as an efficient and durable cathode material. Experimental and theoretical studies confirm the role of S-doping in modifying the electron density distribution of Fe centers, promoting the interaction between the Fe 3d orbital and O 2p orbital of NO3– and thereby enhancing its catalytic performance. A Faradaic efficiency of 93.9% for NH4+ production at − 0.47 V vs. the reversible hydrogen electrode is achieved, which remains at 91.0% even after six cycles. A synergistic effect between a defect-rich support and metal atom centers can be utilized to develop a new strategy for the facile design and implementation of high-performance electrocatalysts.

Liquid Structure and Hydrogen Bonding in Aqueous Hydroxylammonium Nitrate.

Hydroxylammonium nitrate (HAN) has emerged as a promising component in ionic liquid-based spacecraft propellants. However, the physicochemical and structural properties of aqueous HAN have been largely overlooked. The purpose of this study is to investigate the hydrogen bonding in aqueous HAN and understand its implications on these properties and the proton transfer mechanism as a function of concentration. Classical polarizable molecular dynamics simulations have been employed with the APPLE&P force field to analyze the geometry of individual hydrogen bonds and the overall hydrogen-bonding network in various concentrations of aqueous HAN. Radial distribution functions (RDFs) and spatial distribution functions (SDFs) indicate the structural arrangement of the species and their hydrogen bonds. Projections of water density and the orientation of its electric dipole moment near the ions provide insight into the hydrogen-bonding network. The incorporation of water into the hydrogen-bonding network at high ion concentrations occurs via interstitial accommodation around the ions immediately outside the first solvation shell. While ion pairs are observed at all concentrations considered, the frequency of Ha···On hydrogen bonds increases substantially with the ion concentration. The findings contribute to a better fundamental understanding of HAN and the precursors of reactivity, crucial to the development of "green" spacecraft propellants.

Phytochemical Profiling, Green Synthesis, and Bioactivity Evaluation of Silver Nanoparticles (AgNPs) Synthesized from Ipomoea laxiflora Extract

This study focuses on the eco-friendly production of silver nanoparticles using leaf extract from Ipomoea laxiflora and assesses their antioxidant and hemolytic effects. To our knowledge, this is the first report on the synthesis of silver nanoparticles using this species. Green synthesis offers immense potential in both medical and environmental fields, aiming to utilize less hazardous chemicals. Plant-based synthesis, in particular, is considered safe and effective due to the presence of reducing and capping agents within plant extracts. Ipomoea laxiflora H.J. Chowdhery & Debta, belonging to the Convolvulaceae family, is an annual climber native to Tropical Africa and India. It has been traditionally used to treat fever, headaches, and stomach aches. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, phenols, tannins, terpenoids, steroids, glycosides, and cardio glycosides. Quantification of phytochemical contents, including total phenolic, flavonoid, and proanthocyanin content, was also performed. FT-IR spectroscopic analysis indicated characteristic peak values of major functional groups such as alkene, alkane, and carbonyl. Silver nanoparticles were synthesized by adding 10 mL of methanolic leaf extract to 90 mL of 1 mM aqueous silver nitrate solution, followed by heating at 80 degrees Celsius for three hours with continuous stirring. The change in color from yellow to dark brown confirmed the formation of silver nanoparticles. The antioxidant activity, determined by DPPH radical scavenging assay, showed 94% scavenging activity for methanolic extract compared to 98% for ascorbic acid. Higher concentrations exhibited increased scavenging activity. Total antioxidant activity ranged from 60% to 89% in hexane and methanol extracts, with methanol showing the highest concentration. Hemolytic activity was observed between concentrations of 10 to 100 µg/ml, with a hemolysis rate of 2.751% at a concentration of 100 µg/mL. The development of green nanoparticles using natural sources like Ipomoea laxiflora holds significant importance for environmental sustainability, health benefits, diverse biomedical applications, resource efficiency, and cost-effectiveness. Embracing such green approaches not only advances nanotechnology but also aligns with broader goals of promoting sustainable development.

Structural chemistry of penta- and hexanitrato thorium(iv) complexes isolated using N–H donors

Fifteen Th(iv)–nitrate compounds, consisting of [Th(NO3)5(H2O)2]1− or [Th(NO3)6]2− units, were isolated from aqueous solution using a series of N–H heterocycles.

Eggshell-Mediated Hematite Nanoparticles: Synthesis and Their Biomedical, Mineralization, and Biodegradation Applications

The present study demonstrates the synthesis of phase pure hematite (α-Fe2O3) nanoparticles (NPs) using collagen protein and calcium carbonate extracted from eggshell membranes and eggshells, respectively, as organic additives. To test the influence of organic additives on the quality of the resulting NPs, the amount of eggshell powder was varied between 1 to 5 g in aqueous iron nitrate solution. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and RAMAN analysis confirmed the formation of hematite NPs without any impurities. FTIR spectra revealed the presence of polyphenolic constituents on the surface of the resulting NPs as stabilizers, which may potentially be responsible for the observed antioxidant and antibacterial properties. Furthermore, the stable phase and the presence of low defects divulged the high hardness value (~983 HV) and fracture toughness (8.59 MPa m1/2), which can be exploited for bone implantation. The FE-SEM results demonstrate the formation of spherical particles, which are well-separated NPs. The results of a biodegradation study which was carried out in phosphate-buffered saline (PBS) revealed that the as-prepared NPs retained their hardness even after 72 h of soaking. These prepared NPs showed 95% radical scavenging activity (RSA) and were good carriers against S. aureus bacteria. Moreover, the SEM images of the mineralization of iron oxide NPs confirmed the formation of new bone. After 5 weeks, all pores were filled, and the minerals were deposited on the surfaces of the scaffolds.

The proceeded ammonia release from ammonium nitrate owing to sodium malonate addition: Dependence of RH, acidity and surfactant

The gas-particle partition of ammonia has strong effect on size, composition and hygroscopic property of atmospheric aerosols. However, how chemical reaction drives ammonia evaporation from particle is not well known. In this work, ammonium depletion is used to characterize the ammonia liberation from aqueous ammonium nitrate and sodium malonate/ammonium nitrate aerosols. The addition of sodium malonate promotes more ammonia liberation owing to inter-ingredients reaction. As the relative humidity (RH) decreases, ammonia release is enhanced. Moreover, NH3 evaporation is also impacted by aerosol acidity. At pH 1.93, malonate is directly converted into hydrogen malonate and malonic acid via protonation. Under the neutral/alkaline condition, higher pH is beneficial for component reactions. When cetyltrimethylammonium bromide is added into mixed aerosols, NH3 evaporation becomes slow. These results can provide a new perspective on the evolution mechanism of NH3–NH4+ buffer pairs for multicomponent aerosols.

Antibacterial Activity of Green Synthesized Eugenia jambolana Seeds Extract Mediated Silver Nanoparticles

The present study uses silver nanoparticles that are biosynthesized from Eugenia jambolana aqueous silver nitrate solution jambolana fresh seed extracts were combined with silver nitrate (AgNO3) to form the silver nanoparticle. UV-Vis Spectrophotometry, XRD, SEM, and FTIR were used to characterise the synthesised silver nanoparticles. It was established that nanoparticles had formed in the sample by SEM examination, which revealed that they had a spherical shape. Nanoparticles from seed extracts showed more antibacterial activity on Pseudomonas aeruginosa followed by Bacillus subtilis, Vibrio cholerae, and Staphylococcus aureus. This study makes evidence that seed extracts of Eugenia jambolana act as an excellent capping agent for the formation of silver nanoparticles and show immense Pharmacological activities. Hence, these AgNPs can be used as an antimicrobial agent in treating many medical complications. In this present study, the antibacterial activity of green synthesized silver nanoparticles from Eugenia jambolana seeds shows the zone of inhibition against all four pathogens.

Enhanced Corrosion Resistance of TiZrN-Coated Additively Manufactured 8620 Low-Alloy Steel in Nitrate Salt Solution and Salt Bath

In this study, corrosion resistance of TiZrN-coated additively manufactured (3D-printed) 8620 steel has been separately studied in a 60–40 wt% NaNO3 + KNO3 molten salt and in an aqueous nitrate salt environment. Microstructural characterization of the as-built bare 3D-printed 8620 sample revealed martensitic microstructures. High-temperature corrosion analysis in molten nitrate salt revealed improved film stability and corrosion resistance of the coating. The coated 3D-printed sample showed no scale of corrosion products, whereas the bare 3D-printed sample exhibited a layer of corrosion products of 2.62 ± 0.24 µm thickness. Potentiodynamic polarization and electrochemical impedance spectroscopy tests in aqueous nitrate salts at room temperature exhibited an ~8-fold decrease in corrosion current density and a ~7-fold increase in charge transfer resistance, indicating enhanced corrosion resistance of the coated 8620. The coated wrought 8620 showed comparable corrosion resistance to that of the coated 3D-printed sample. However, in aqueous solution, the bare 3D-printed sample exhibited localized corrosion, whereas the bare wrought revealed uniform corrosion on the surface.

Effect of manganese addition to Ni-Cu oxides on properties and activity in the oxidation of volatile organic compounds

Transition metal oxides are suitable for the total oxidation of harmful volatile organic compounds. The effect of Mn addition to Ni-Cu oxides was investigated to determine the optimum cation composition for achieving high catalytic performance in the total oxidation of ethanol and toluene, which were chosen as model volatile organic compounds. Single component (Ni, Cu, Mn), binary Ni-Cu, and ternary (Ni-Cu-Mn) oxide catalysts with various Ni:Cu and Ni:Cu:Mn molar ratios were prepared by precipitation of aqueous nitrate solutions followed by calcination at 500 °C in air. The catalysts were characterized by AAS, powder XRD, Raman spectroscopy, N2 adsorption, H2-TPR, and XPS spectroscopy. The addition of Mn to Ni-Cu oxides led to the formation of Ni-Cu-Mn mixed oxides with spinel structure and Ni-Mn mixed oxides (Ni6MnO8, NiMnO3). On the other hand, the Ni-Cu catalysts contained a mixture of NiO and CuO. In the H2-TPR experiments, the Ni-Cu-Mn oxides were reduced at lower temperatures compared to the binary Ni-Cu catalysts. The catalytic performance of Ni-Cu-Mn oxide catalysts was higher than that of the Ni-Cu catalysts. As the Mn concentration increased, the concentration of metal-bound oxygen raised, while the concentration of oxygen vacancies slightly decreased. A linear correlation was revealed between the surface concentrations of oxygen vacancies determined by XPS and specific reaction rates in ethanol and toluene oxidation over the Ni-Cu and Ni-Cu-Mn catalysts. For the Ni-Cu-Mn mixed oxides and the mixture of Ni and Cu oxides, a synergistic effect was found in the oxidation of both model compounds.

Development and verification of a one-dimensional collision probability based neutron transport code to model axially heterogeneous cylindrical vessels containing aqueous and organic plutonium nitrate

This paper presents the development and verification of a collision probability (CP) code, capable of modelling neutron transport in one-dimensional slabs and axially heterogeneous cylinders with varying radii. The CP code is used to model layered systems of aqueous and organic plutonium nitrate, as process criticality accidents are more likely to occur in these systems compared to dry systems. The use of the CP code is desired as it offers a computationally inexpensive method for calculating neutron transport when compared to higher fidelity codes such as MCNP. For slab geometries, the CP code can be used effectively, given they contain at least 0.7 g cm−2 plutonium. The approximation employed by the CP code to model heterogeneous cylinders overestimated the rate of radial neutron leakage such that vessels with radii of 30.0 cm could not reliably calculate reactivity to within 1 $ of MCNP. Increasing the radii to 40.0 cm improved the accuracy of the CP code to within 1 $ of MCNP for systems containing at least 2.75 kg plutonium. The error in the CP code increased when used to model cylindrical geometries with dished ends and complete vessels with dished ends, such that systems with large dished ends and low plutonium content should be avoided. As a simple, neutronics based model, the CP code could be used as part of rough order of magnitude calculations for criticality transients, where high levels of accuracy are not required, given that potential errors in results have been previously identified.

Preparation of Ag@lignin nanotubes for the development of antimicrobial biodegradable films from corn straw

Current environmental and energy issues have attracted considerable attention from industries, governments, and academia. Developing alternative diverse petrochemical-based plastics with biodegradable packaging materials from renewable resources is critical for ensuring both sustainability and safety. In this study, biodegradable films are fabricated from corn straw via a facile sol-gel process. Furthermore, these films are imbued with antimicrobial properties by coupling with silver@lignin nanotube hybrid antibacterial agents, formed via the in situ reduction of silver ions into elemental silver by lignin (mild reducing agent), followed by the self-assembly of lignin molecules into nanotubes assisted by an aqueous silver nitrate electrolyte solution. The developed antibacterial corn straw film exhibits strong mechanical and antibacterial properties, with a tensile strength and elongation at break of 68.7 MPa and 11.3 %, respectively, under optimum conditions and antibacterial activity against Escherichia coli and Staphylococcus aureus of 99.9 % and 97.2 %, respectively. The as-prepared corn straw films exhibit high hydrophobicity and ultraviolet resistance. The morphology, structure, and thermal properties of the corn straw films were characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction, and thermogravimetric analysis. This study provides a straw-based biodegradable packaging film with antimicrobial properties.

Growth of large norsethite crystals in aqueous ammonium nitrate solutions

Crystallization of norsethite [BaMg(CO3)2] receives considerable attention as a key for solving geological problems, and environmental and resource issues. Noresthite is poorly soluble in water, and is synthesized via witherite [BaCO3] by solution-mediated transformation. In contrast, owing to the difficulties such as tremendous growth time and consideration of multi-step reactions, the shape and size of norsethite were not satisfactory for further investigations so far. Here we tried to increase the solubility of the crystals to overcome these difficulties of the growth by adding NH4NO3. The solubilities of norsethite and witherite in the presence of NH4NO3 were ∼35 and ∼45 times higher than those in the absence of NH4NO3, respectively. At 90 °C, the growth rate of norsethite in NH4NO3 solutions was ∼17 times higher than that in NH4NO3-free solutions, and norsethite in NH4NO3 solutions nucleated immediately after mixing the raw materials. Finally, we succeeded in synthesizing bulky norsethite crystals of 170 μm size only in one month, utilizing 0.1–1 M NH4NO3 solutions of 90 °C. We could explain the appearance of large norsethite, considering that norsethite dominantly consumes the solute by adding NH4NO3 compared with witherite. The findings provide a breakthrough in the growth technique of double carbonates.

Effects of silver nanoparticles prepared by aqueous extract of Ferula communis on the developing mouse embryo after maternal exposure.

Green synthesis of silver nanoparticles (AgNPs) from aqueous silver nitrate has been achieved using an extract of Ferula communis leaf as a capping, reducing, and stabilizing agent. The formation and stability of the green synthesized silver nanoparticles in the colloidal solution were monitored by absorption measurements. Silver nanoparticles were characterized by different analyses such as X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), and FT-IR spectroscopy. The average particle size of silver nanoparticles was determined by high-resolution transmission electron microscopy (HRTEM) and scanning electron microscopy (SEM) analyses. In this experiment, pregnant female mice were divided into four groups (G); G1 was the control and received phosphate-buffered saline, G2 received orally aqueous extract of F. communis leaf, G3 received orally AgNPs chemically prepared by NaBH4, and G4 received orally AgNPs prepared by aqueous extract of F. communis leaf. The diameter of AgNPs was 20nm. AgNPs exhibited good catalytic reduction ability toward methyl orange in the presence of sodium borohydride with a rate constant of 2.95 x 10-4 s-1. The results revealed the occurrence of resorbed embryos in G2, G3, and G4 with different percentages. The livers of mothers and embryos at E14.5 in G2, G3, and G4 showed different levels of histopathological alteration and increase in GFAP and CTGF expressions compared with the control group. The study concluded that the oral administration of small-sized AgNPs (20nm) prepared by Ferula extract had less toxicity than those prepared by the chemical method.

Nitrate Uptake by Cellulose-Based Anion Exchange Polymers Derived from Wheat Straw

Nitrate contamination of ground water is a serious problem due to the intensive agricultural activities needed to feed the world’s growing population. While effective, drinking water treatment using commercial ion exchange polymers is often too expensive to be employed. At the same time, lignocellulosic waste from crop production—an abundant source of the renewable polymer cellulose—is often burned to clear fields. This results in not only adverse health outcomes, but also wastes a valuable resource. In this study, wheat straw was pretreated to extract cellulose, then selectively oxidized with periodate, crosslinked with an alkyl diamine (1,7-diaminoheptane or 1,10-diaminodecane), and functionalized with a quaternary ammonium compound ((2-aminoethyl)trimethyl ammonium chloride) to generate a cellulose-based anion exchange polymer. This polymer lowered aqueous nitrate concentrations to health-based drinking water standards. Unlike commercial ion exchange polymers, its synthesis did not require the use of toxic epichlorohydrin or flammable solvents. The pretreatment conditions did not significantly affect nitrate uptake, but the crosslinker chain length did, with polymers crosslinked with 1,10-diaminodecane showing no nitrate uptake. Agricultural-waste-based anion exchange polymers could accelerate progress toward the sustainable development goals by providing low-cost materials for nitrate removal from water.

Study of plutonium recovery from acidic feed using polyethersulfone encapsulating TBP, DEHPA, and mixture of TBP and DEHPA beads

ABSTRACT Polyethersulfone (PES) composite beads encapsulating the extractants di-(2-ethylhexyl) phosphoric acid (DEHPA), tributyl phosphoric acid (TBP), and mixture of DEHPA & TBP were synthesized. The synthesis was carried out using phase inversion method. Subsequently, these PES beads were applied for the recovery of plutonium [Pu (IV)] from different aqueous acidic medium. In kinetic studies, the extraction of Pu (IV) was found to be fast and equilibrium was attained within 5 minutes. In batch sorption studies, the role of aqueous acidity and nitrate ion concentration on extraction efficiency was evaluated. Also, the effects of variation of acid medium, concentration of metal ion, extractant concentration were examined for Pu (IV) recovery. Back extraction was effectively carried out using oxalic acid. The reusability of the PES beads was tested for 10 extraction – stripping cycles.

Ecofriendly Green Synthesis and Characterisation of Silver Nanoparticles of Hyoscyamus Niger Seeds and Evaluation of its Anthelmintic Activity

Introduction: Nanotechnology is the design, characterisation and production of molecules or structures at nanometer scale. Metallic nanoparticles have been known for their therapeutic applications for thousands of years. Hyoscyamus niger is a traditional plant used as analgesic, sedative, anthelmintic, antitumour and febrifuge. Objective: An eco-friendly approach was made for the synthesis of silver nanoparticles using aqueos seed extract of Hyoscyamus niger (H.n-AgNPs) and checked for possible anthelmintic activity. Method: Preparation of silver nanoparticles in 1 mM aqueous silver nitrate from Hyoscyamus niger seed. The color shift from yellow to brownish red provided visible evidence of nanoparticle production. UV visible, Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray diffraction (XRD) examinations all validated it further. We tested the in vitro anthelmintic activity of various doses of H.n-AgNPs on Indian earthworms.. Results: The size of the H.n-AgNPs synthesized ranged between 8nm to183 nm. Synthesized NPs showed potential anthelmintic activity at 100µg/mlwith paralysis time and death time of 15 min and 34 min respectively where Albendazole was used as standard drug. The phytoconstituents like tannins and phenolic compounds may be responsible for precipitation of proteins or can attach with free proteins in gastrointestinal tract or glycoprotein on parasite cuticle and cause cell death. Conclusion: The H.n-AgNPs synthesized have potential anthelmintic activity which was concentration dependent. Further research will be done to design a new anthelmintic drug using H.n-AgNPs.