Aqueous Ag Research Articles

Green Formulation of Descurainia sophia Aqueous Extract Mediated Ag NPs and Determination of Its Anti-Hemangioma Effects

Hemangioma is a common cancer with severe clinical signs. Finding a new anti-hemangioma supplement or drug inside of the chemotherapeutic drug is valuable. We synthesized silver nanoparticles containing Descurainia sophia leaf for investigating the anti-hemangioma, cytotoxicity, and antioxidant effects. Characterization of AgNPs was performed by Field Emission Scanning Electron Microscopy (FE-SEM), UV–Visible Spectroscopy (UV-Vis), and X-Ray diffraction analysis (XRD). The plain peak in the wavelength 449 nm revealed the AgNPs synthesis. TEM and FE-SEM analyses showed that synthesized AgNPs were found to be 16.9 to 48.43 nm mean particle size. For investigating the antioxidant efficacy, the DPPH assay was conducted. AgNPs prevented 50% of DPPH in the dilation of 40 μg/mL. To measure the anti-hemangioma efficacy of AgNPs, MTT assay was applied on SK HEP-1 and HAEND cell lines. AgNPs had high anti-hemangioma efficacy on above tumor cells without any significant toxicity on HUVEC. The best finding of anti-hemangioma properties of AgNPs was seen in the case of the HAEND cell line. This study indicated significant anti-hemangioma and antioxidant potentials of AgNPs containing Descurainia sophia.

Solution-processable polymers of intrinsic microporosity for selective silver removal from aqueous solution and subsequent disinfection by silver-laden coatings

Silver ions (Ag+) in waterways pose a growing threat to the environment and living organisms. Achieving an effective and green process for Ag+ removal requires an adsorbent with excellent specificity for silver ions and easy recoverability after fouling or blocking. However, this remains a major challenge. To address this concern, we developed a high-performance adsorbent by grafting polymers of intrinsic microporosity (PIM) with thioamide groups. This thioamide-modified PIM (TPIM-1) adsorbent exhibited an exceptional adsorption capacity of 656 mg g−1 and high selectivity (distribution coefficient of 833.3 mL g−1, selectivity coefficient of 346 over Cd2+) for aqueous Ag+ removal. TPIM-1 also features good solubility in selected solvents, allowing regeneration through a dissolution separation precipitation (DSP) procedure once fouling or blocking occurs. Over 80 % of the adsorption capacity was recovered after nine continuous regeneration cycles, showcasing potential cost-effective reusability. Density Functional Theory (DFT) results reveal that the grafted thioamide groups play a critical role in selective Ag+ adsorption, with a binding energy of 54 kcal mol−1. In addition, the Ag+-loaded PIM-coated glass surface showed excellent antibacterial ability, inhibiting the growth of Bacillus subtilis and E. coli. This study provides a viable strategy for the development of a low-carbon footprint silver removing adsorbent from wastewater.

Adsorption performance of sulfur-decorated polyamidoamine dendrimers/silica for Ag(I) and Cu(II)

Water pollution aroused by heavy metal poses great damage to environmental safety. Polyamidoamine (PAMAM) dendrimers display great advantages in preparation efficient adsorbents to decontaminate heavy metal ions. Herein, sulfur-decorated PAMAM dendrimers/silica (G0-MT, G1-MT and G2-MT) were constructed by using alkoxysilyl-containing PAMAM dendrimers as precursor. G0-MT, G1-MT and G2-MT were employed for removing Ag(I) and Cu(II) from water solution. The adsorption capacity for Ag(I) and Cu(II) follows the order of G0-MT < G2-MT < G1-MT and G0-MT < G1-MT < G2-MT, respectively. The maximum adsorption capacity of the composites for Ag(I) and Cu(II) are 1.38 and 0.78 mmol g−1, which are superior to the corresponding adsorbents that synthesized by chemical modification method. Adsorption kinetic indicates the saturated adsorption for Ag(I) and Cu(II) can be reached at 150 and 270 min. The adsorption for the ions could be boosted by raising metal ion concentration and adsorption temperature. Adsorption mechanism demonstrates S atoms dominate the adsorption, while O and N atoms participate in the adsorption. The noticeable adsorption capacity and regeneration property enable the composites to be promising candidate for efficient removing aqueous Ag(I) and Cu(II).

Synthesis of polyhydroxyl-capped PAMAM dendrimer/silica composites for the adsorption of aqueous Hg(II) and Ag(I)

Water pollution caused by Hg(II) and Ag(I) poses deleterious effects to environmental safety. Adsorption is one of the promising methods to decontaminate aqueous metal ions. Herein, polyhydroxyl-capped poly(amidoamine) (PAMAM) dendrimer/silica composites (G1-OH and G2-OH) were prepared for decontaminating aqueous Hg(II) and Ag(I). The maximum adsorption capacity of G1-OH and G2-OH for Hg(II) are 0.45 and 0.76 mmol/g, while that for Ag(I) are 0.66 and 0.81 mmol/g. The optimum solution pH for the adsorption of Hg(II) and Ag(I) are both 6. The adsorption for Hg(II) and Ag(I) can reach equilibrium at 150 and 120 min, respectively. Pseudo-second-order model can be used to describe the adsorption kinetic process and the rate-controlling step is film diffusion process. Adsorption isotherm indicates the adsorption can be promoted by increasing concentration and temperature, and the adsorption process could be described by Langmuir model with chemical mechanism. G1-OH and G2-OH exhibit excellent adsorption selectivity and they can 100% adsorb Hg(II) or Ag(I) with the coexisting of Fe(III), Co(II), Cu(II) or Ni(II). Adsorption mechanism confirms C-N, OH and CONH groups play critical role for the adsorption of the two ions. The work may provide efficient adsorbents for the decontamination of aqueous Hg(II) and Ag(I) with practical value.

The cytotoxic, genotoxic and mitotoxic effects of Atractylis gummifera extract in vitro.

The Mediterranean thistle Atractylis gummifera L. (Asteraceae; AG) has diterpenoid glucosides; atractyloside and carboxyatractyloside that interact with mitochondrial protein adenine nucleotide translocator (ANT) and resulted in ATP inhibition. Despite its well-known toxicity, acute poisonings still occur with this plant. Although most symptoms are attributed to ANT and diterpenoids interaction, in-depth investigation of the effects of AG extract on various cellular processes has not been performed. We tested in vitro induction of mitochondrial permeability transition pore (MPTP) opening in bovine liver mitochondria and evaluated its cytotoxicity and genotoxicity using Allium cepa test. Cell division, mitotic index (MI) and total chromosomal and mitotic aberrations (TAs), that all seem potentially affected by ATP shortage, were studied in root cells of Allium cepa exposed to Atractylis gummifera extract. With the two different doses of two purified AG fractions, stronger induction of MPTP was observed compared to the induction with the standard pure atracyloside. Aqueous AG extract exerted inhibition root growth in A. cepa at 6 different doses. The TAs was increased in a dose-dependent manner too, while mitotic index was decreased at the same doses. Evaluation of mitotic phases revealed mitodepressive effect of AG on A. cepa roots. this work highlights cellular and mitochondrial adverse effects of Atractylis gummifera extracts. A purified fraction that likely corresponds to ATR derivatives induces MPTP opening leading to swelling of mitochondria and its dysfunction. Allium cepa test provides the evidence for A. gummifera genotoxicity and cytotoxicity.

A bioinspired and sustainable route for the preparation of Ag-crosslinked alginate fibers decorated with silver nanoparticles

Functional materials obtained through green and sustainable routes are attracting particular attention due to the need to reduce the environmental impact of the chemical industry. In this work we propose a bioinspired approach for the preparation of alginate fibers containing silver nanoparticles (AgNPs), to be used for antimicrobial purposes. We demonstrate that filiform polymeric structures with length of a few meters can be easily obtained by extruding an alginate solution in an aqueous Ag+-containing bath (i.e. wet spinning) and that treating the fibers with freshly-squeezed lemon juice leads to the formation of AgNPs homogeneously distributed within the polymeric network. Using mixtures of ascorbic and citric acid to mimic lemon juice composition we highlight the influence of the aforementioned molecules on the nanoparticles formation process as well as on the properties of the fibers. Varying the amount of citric and ascorbic acid used for the treatment allows to finely tune the thermal, morphological and water absorption properties of the fibers. This evidence, along with the possibility to easily monitor the preparation through FT-IR spectroscopy, endows the fibers with a high application potential in several fields such as wound healing, water/air purification and agriculture.

Direct sunlight induced room temperature synthesis of anticancer and catalytic silver nanoparticles by shrimp shell waste derived chitosan

The use of marine waste derived chitosan (CS) for the synthesis of nanomaterials is considered as one of the effective routes for bio-waste management and recovering functional products. Herein, CS capped silver nanoparticles (Ag NPs-CS) with potential anticancer and dye pollutants adoption properties have been synthesized photochemically under direct sunlight. To obtain, CS, shrimp shell waste was subjected to a serious of standard demineralization, deproteinization and deacetylation processes. The electronic absorption peak (400 nm) denoting surface plasmonic resonance of Ag NPs and infrared peaks relevant to CS (3364 cm−1 of OH/NH2, 2932 cm−1 of CH, and 1647 cm−1 of -CO) exhibited peaks confirmed the formation of CS-Ag NPs. Ag NPs-CS exhibited anticancer activity against Human lung adenocarcinoma cell lines (A549), the maximum cell death noticed at the concentration of 20 μg/mL and 70 μg/mL was 20 and 52 %, respectively. An aqueous Ag NPs-CS (100 μg/mL) was degraded ≥95 % of mixed dye target solution (25 mg/mL) containing equal volume of cationic dye (Methylene blue and Rhodamine B) and anionic dye (methyl orange). Therefore, these findings suggest that the shrimp shell waste derived CS can be used for the synthesis of CS-Ag NPs with potential biomedical and environmental applications.

Selective and high capacity recovery of aqueous Ag(I) by thiol functionalized mesoporous silica sorbent

A simplified sol–gel method was developed for the preparation of thiol functionalized porous silica particles using (3-mercaptopropyl)-trimethoxy-silane (MPTMS) for co-gelation in the presence of micellar templates. The resulting short range ordered porous silica microparticles are exceptionally effective sorbents of aqueous Ag(I). The binding of aqueous Ag(I) is almost stoichiometric in the broad pH range between 4.0 and 9.0 even at low Ag(I) concentrations until reaching the limiting 238 mg g−1 sorption capacity. The sorbent displays high selectivity towards Ag(I), which was tested in the simultaneous presence of several different metal compounds. The practically complete recovery of Ag(I) and the regeneration of the sorbent was achieved by washing with a 10 mM Na2S2O3 solution. X-ray photoelectron spectroscopy (XPS) revealed that silver is not reduced on the surface of the sorbent. The reason for the strong and selective binding of Ag(I) is the formation of layered thiolate coordination compounds on the functionalized silica surface that display characteristically high stability even in the presence of other competing metal ions.

Allium ampeloprasum leaf aqueous extract green-formulated Ag nanoparticles: Determination of anti-human lung cancer and antioxidant effects

Allium ampeloprasum as an ethnomedicinal plant has many therapeutic effects in the traditional medicine. In the recent years, the silver nanoparticles green-mediated by ethno medicinal plants have been used for the treatment of several kinds of cancers. Now, we investigated the silver nanoparticles green-synthesized according to green chemistry rules using the aqueous extract of Allium ampeloprasum leaf for the treatment of lung carcinoma. The properties of silver nanoparticles against common human lung cancer cell lines i.e. HT144, SKMEL2, WM266-4, and IPC-298 were evaluated. The green-synthesized silver nanoparticles were characterized using different techniques such as FE-SEM, UV-Vis, and TEM. The FE-SEM and TEM results confirm spherical morphology for the nanoparticles with size of 11.9–46.7 nm. The IC50 of the silver nanoparticles was 125, 164, 180 and 149 µg/ml against HT144, SKMEL2, WM266-4, and IPC-298, respectively. After doing the clinical trial studies, the recent nanoparticles may be used as an anti-lung supplement in humans.

Particle size and temperature effects on thermal conductivity of aqueous Ag nanofluids: modelling and simulations using classical molecular dynamics

Particle size and temperature effects on thermal conductivity of aqueous Ag nanofluids: modelling and simulations using classical molecular dynamics

Green capturing of Ag from ultra-low concentration precious metal wastewater by electrodeposition assisted with electrocoagulation: Electrochemical behavior and floc characterization

As an environmental-friendly water treatment technique, direct electrodeposition (ED) encountered some difficulties to recover low-concentration metallic ions. This work proposed a novel electrodeposition assisted with electrocoagulation (ED-EC) process to remove and capture Ag+ from ultra-low concentration wastewater. Influences of operating parameters on the removal efficiency were investigated. Results showed that the Ag+ removal ratio of ED-EC increased to > 97.4 % compared to direct ED (<90 %), and Ag+ was reduced to < 0.1 ppm from an initial concentration of 2–20 ppm. Low current density, low pH and high Ag+ concentration improved electrodeposition, but weakened the formation and adsorption of the flocs by electrocoagulation. Aqueous Ag+ was captured and enriched in the flocs and the cathode deposit, where the Ag contents were > 3000 g/t and > 97 wt%, respectively. The economic assessment indicated that recovering Ag by ED-EC produced considerable benefits. Thus, this study provides meaningful guidance for resource recovery from precious metal wastewater.

Oxidation of organic micropollutant surrogate functional groups with peracetic acid activated by aqueous Co(II), Cu(II), or Ag(I) and geopolymer-supported Co(II)

Peracetic acid (PAA) in combination with transition metals has recently gained increasing attention for organic micropollutant abatement. In this study, aqueous Co(II), Cu(II), and Ag(I) were compared for their capacity to activate PAA. Co(II) outperformed Cu(II) or Ag(I) and the optimum conditions were 0.05 mM of Co(II), 0.4 mM of PAA, and pH 3. However, due to a wider applicability in water treatment, pH 7 (i.e., bicarbonate buffer) was selected for detailed investigations. The abatement of different micropollutant surrogates could be described with a second-order rate equation (observed second-order rate constants, kobs were in the range of 42–132 M−1 s−1). For the para-substituted phenols, there was a correlation between the observed second-order rate constants of the corresponding phenolates and the Hammett constants (R2 = 0.949). In all oxidation experiments, the reaction rate decreased significantly after 1–2 min, which coincided with the depletion of PAA but also with the deactivation of the Co(II) catalyst by oxidation to Co(III) and subsequent precipitation. It was demonstrated that Co(II) immobilized on a geopolymer-foam performed approximately similarly as aqueous Co(II) but without deactivation due to Co(III) precipitation. This provides a potential option for the further development of heterogeneous catalytic Co(II)/PAA advanced oxidation processes utilizing geopolymers as a catalyst support material.

Feasible fabrication of o-phenanthroline-based polymer adsorbent for selective capture of aqueous Ag(I)

Devising a desirable adsorbent for efficiently selective capture of Ag(I) from wastewater has attracted much attention but faced with huge challenges. Herein, a novel linear o-phenanthroline-based polymer l-PRL was prepared via chemical oxidative polymerization for the adsorption of Ag(I). The maximum adsorption capacity for Ag(I) by l-PRL is 325.8 mg/g at pH 0. In addition, l-PRL owes ascendant selectivity for Ag(I) from aqueous solutions containing various interfering metal ions of Pb(II), Co(II), Ni(II), Cd(II) and Fe(III). Multiple characterizations of FT-IR and XPS uncover that the N groups on l-PRL act as adsorption sites to coordinate with Ag(I). Density functional theory (DFT) calculations further evidence the mechanism that l-PRL is provided with the admirable adsorptivity and selectivity for Ag(I). It is mainly attributed to the most stable complexes of l-PRL with Ag(I), which possesses shortest Ag-N bond length compared with other heavy metal ions. Furthermore, 93.5% of initial adsorption capacity is reserved after four continuous regeneration cycles, indicating that l-PRL is equipped with superior recyclability and durability, and l-PRL is capable of removing Ag(I) in low-concentration actual Ag(I)-containing wastewater completely. This study shed light on the rational design of polymer adsorbents and in-depth insight into selective removal of aqueous Ag(I).

Synthesis and Mechanism of Spherical Ag-doped Polypyrrole Assisted by Complexing Agents

Highly dispersed Ag-doped Polypyrrole (PPy) spherical composites can be efficiently synthesized via oxidative polymerization of pyrrole with FeCl3 in an aqueous Ag+-containing solution in the presence of trisodium citrate, followed by concentrated ammonia treatment. However, the formation mechanisms involved in how to control the shape and how to get the metallic Ag0 need further investigation. In order to elucidate the formation mechanisms, the intermediates in different reaction stage were collected and investigated. Combining the experimental phenomenon and the structure characterization of the samples, it was found that citrate ions make a role of complexing Ag+ to produce [Ag3(C6H5O7)n+1]3n− complexes in the early reaction stage, then mainly play a role of steric stabilizer of AgCl micelles and are responsible for the shape tailoring of PPy composite as well as the reduction of Ag+ in the process of ammonia treatment. Evidently, negative-charged AgCl micelles become the main nucleation sites of pyrrole polymerization through the electrostatic attraction between the negative and positive ions. Concentrated ammonia is adopted to eliminate AgCl cores from the precursor of Ag-doped PPy composites obtained by chemical redox reaction and provides an accelerated reaction condition for reduction of Ag+ by reductants (citrate ion or pyrrole monomer). Ag-containing micelles induction method is a facial chemical method to obtain uniform Ag-doped composites and can be broadened to design other Ag-containing functional materials.

Adsorption of aqueous Cu(II) and Ag(I) by silica anchored Schiff base decorated polyamidoamine dendrimers: Behavior and mechanism

A class of silica anchored Schiff base decorated polyamidoamine (PAMAM) dendrimers were synthesized for removing aqueous Cu(II) and Ag(I). The adsorption performance was investigated synthetically and the adsorption mechanism was revealed. Results indicate the adsorption capacity depends on dendrimer generation, solution pH, contact time, temperature and initial metal ion concentration. The optimum adsorption pH is 6 for both metal ion. Adsorption kinetic suggests the adsorption can achieve equilibrium at 180 and 150 min for Cu(II) and Ag(I). The kinetic process is found to be in good agreement with pseudo-second-order model and film diffusion is the rate-controlling step. The adsorption isotherm indicates the adsorption is proceeded by monolayer behavior with chemical mechanism. These adsorbents exhibit competitive adsorption capacity as compared with other reported adsorbents. Theoretical calculation demonstrates the participation of hydroxyl, carbonyl, and amide groups during the adsorption of Cu(II), while hydroxyl and amide groups are mainly responsible for capturing Ag(I).

Biosynthesis of Silver Nanoparticles from Citrobacter freundii as Antibiofilm Agents with their Cytotoxic Effects on Human Cells

Nanomaterials have recently been identified for their potential benefits in the areas of medicine and pharmaceuticals. Among these nanomaterials, silver nanoparticles (Ag-NPs) have been widely utilized in the fields of diagnostics, antimicrobials, and catalysis. To investigate the potential utility of Citrobacter freundii in the synthesis of silver Nanoparticles (Ag-NPs), and to determine the antimicrobial activities of the Ag-NPs produced. Aqueous Ag+ ions were reduced when exposed to C. freundii extract and sunlight, leading to the formation of Ag-NPs. Qualitative microanalysis for the synthesized Ag-NPs was done using UVvis spectrometry, Energy Dispersive X-ray analysis (EDX), and scanning and transmission electron microscopy. The hydrodynamic size and stability of the particles were detected using Dynamic Light Scattering (DLS) analysis. The Ag-NPs' anti-planktonic and anti-biofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa, which are two important skin and wound pathogens, were investigated. The cytotoxicity on human dermal fibroblast cell line was also determined. Ag-NPs were spherical with a size range between 15 to 30 nm. Furthermore, Ag-NPs displayed potent bactericidal activities against both S. aureus and P. aeruginosa and showed noticeable anti-biofilm activity against S. aureus biofilms. Ag-NPs induced minor cytotoxic effects on human cells as indicated by a reduction in cell viability, a disruption of plasma membrane integrity, and apoptosis induction. Ag-NPs generated in this study might be a future potential alternative to be used as antimicrobial agents in pharmaceutical applications for wound and skin related infections.

High-Throughput Time-Resolved Photoluminescence Study of Composition- and Size-Selected Aqueous Ag–In–S Quantum Dots

Brightly luminescent, composition- and size-selected aqueous Ag–In–S (AIS) and Zn-diffused core/shell AIS/ZnS (ZAIS) quantum dots (QDs) were studied by high-throughput time-resolved photoluminescence (TRPL). Both QD size and QD composition effects on the photophysical properties of AIS (ZAIS) were probed independently by using an automated high-throughput TRPL setup. Linear relationships were found between the average PL lifetimes of AIS and ZAIS QDs and their respective bandgaps (Eg) indicating that the dynamics of the radiative recombination is governed mostly by the energy of the QD excited state irrespective of which factor is varied affecting the QD bandgap, i.e., their size or composition. The rate constants of radiative and nonradiative recombination were evaluated as a function of Eg forming continuous dependences for the entire AIS and ZAIS QD families. The rate of nonradiative recombination increases steeply for smaller QDs with Eg > 2.2 eV for both core AIS and core/shell ZAIS QDs, indicating that the interfacial electron transfer is the major contributor to the observed dependence. A strong decrease of the PL lifetime of AIS (and ZAIS) QDs in dense QD films or QDs incorporated into polyvinylpyrrolidone films was observed as compared to colloidal solutions. A reversible character of this effect upon polymer film dissolution shows that such behavior originates from energy (or electron) transfers among the QDs brought into close contact in the films. This phenomenon is expected to be of significance for the application of ternary QDs as a light-harvesting material.

Efficient electrocatalytic nitrogen reduction to ammonia with aqueous silver nanodots

The electrocatalytic nitrogen (N2) reduction reaction (NRR) relies on the development of highly efficient electrocatalysts and electrocatalysis systems. Herein, we report a non-loading electrocatalysis system, where the electrocatalysts are dispersed in aqueous solution rather than loading them on electrode substrates. The system consists of aqueous Ag nanodots (AgNDs) as the catalyst and metallic titanium (Ti) mesh as the current collector for electrocatalytic NRR. The as-synthesized AgNDs, homogeneously dispersed in 0.1 M Na2SO4 solution (pH = 10.5), can achieve an NH3 yield rate of 600.4 ± 23.0 μg h−1 mgAg−1 with a faradaic efficiency (FE) of 10.1 ± 0.7% at −0.25 V (vs. RHE). The FE can be further improved to be 20.1 ± 0.9% at the same potential by using Ti mesh modified with oxygen vacancy-rich TiO2 nanosheets as the current collector. Utilizing the aqueous AgNDs catalyst, a Ti plate based two-electrode configured flow-type electrochemical reactor was developed to achieve an NH3 yield rate of 804.5 ± 30.6 μg h−1 mgAg−1 with a FE of 8.2 ± 0.5% at a voltage of −1.8 V. The designed non-loading electrocatalysis system takes full advantage of the AgNDs’ active sites for N2 adsorption and activation, following an alternative hydrogenation mechanism revealed by theoretical calculations.

Mechanism of Gold-Silver Alloy Nanoparticle Formation by Laser Coreduction of Gold and Silver Ions in Solution.

Photochemical reduction of aqueous Ag+ and [AuCl4]- into alloy Au-Ag nanoparticles (Au-Ag NPs) with intense laser pulses is a green synthesis approach that requires no toxic chemical reducing agents or stabilizers; however size control without capping agents still remains a challenge. Hydrated electrons produced in the laser plasma can reduce both [AuCl4]- and Ag+ to form NPs, but hydroxyl radicals (OH·) in the plasma inhibit Ag NP formation by promoting the back-oxidation of Ag0 into Ag+. In this work, femtosecond laser reduction is used to synthesize Au-Ag NPs with controlled compositions by adding the OH· scavenger isopropyl alcohol (IPA) to precursor solutions containing KAuCl4 and AgClO4. With sufficient IPA concentration, varying the precursor ratio enabled control over the Au-Ag NP composition and produced alloy NPs with average sizes less than 10 nm and homogeneous molar compositions of Au and Ag. By investigating the kinetics of Ag+ and [AuCl4]- coreduction, we find that the reduction of [AuCl4]- into Au-Ag NPs occurs before most of the Ag+ is incorporated, giving us insight into the mechanism of Au-Ag NP formation.

Biosynthesis of silver nanoparticles produced by Klebsiella pneumoniae

This study was designed to isolate Klebsiella pneumoniae from human and sheep and Biosynthesis of silver nanoparticles produced by klebsiella pneumoniae , the aqueous Ag + ions were reduced during exposure to the culture supernatant of Klebsiella pneumoniae . The formation of nanoparticles showed change in colour of the reaction solution turned from yellow to brown, and using The Atomic Force Electron Microscopy (AFM) micrograph for concentration (1 mM) silver nanoparticles indicates the surface changes where root mean square (RMS) values equal to 6.03 nm and average roughness of 5.43 nm also the surface thickness equal to 22.94 nm and Average grin size value was 67.40 nm. but the concentration (10 mM) of silver nanoparticles, were indicates the surface changes root mean square (RMS) values is equal 9.32 nm and roughness average was 3.47 nm also the surface thickness equal 33.34 nm and Average grin size value was 63.03 nm. The analysis image of silver nanoparticles shows that they have a cube shape with small pieces and irregular heterogeneous forms. The average size of the minutes ranges between 40.47_89 nm. at concentration (1 Mm). and image of Transmission electron microscopy shows a pseudo- spherical shape (1 mM) which shows the nanoparticle size ranging from 40 to 80 nm. Also Transmission electron microscopy shows a pseudo-spherical shape of the focus (10 mM) which shows the nanoparticle size ranging from 60 to 100 nm.