Manipulation of microparticles in heterogeneous complex colloids has become important in various research fields that use microfluidic devices, such as biochemical analyses and clinical diagnosis. Although various techniques for microparticle manipulation in microfluidics have been developed, further advancements are still required for highly accurate analyses. Microparticle manipulation techniques, which include microparticle focusing, tweezing, and separation, using surface acoustic waves (SAWs) have emerged and gained attention. Since SAW-based microfluidics has advantages of being non-invasive, being harmless to particles, and consuming low power intensity and so on, it has great potential for further advancements, especially for biochemical research field. Recently, SAW-based techniques which can manipulate a variety of microparticles have been developed in my group. To predict the behavior of microparticles in microchannel flow, advanced analytical model was developed, and validated with experimental results. In the experiment, the heterogeneous sample, which includes blood, engineering particles, encapsulated cells, sperms, etc., were separated successfully into homogeneous sample with the separation efficiency over 99%. In this presentation, developments of SAW-based microparticle manipulation techniques to date and recent results of my group will be reviewed. In addition, some recommendations for future work of novel applications of SAW will be suggested. Acknowledgement This research was supported by Nano. Material Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No. 2011-0020090)