Apple Extracts Research Articles

A FORMULATION OF MANALAGI (Pyrus malus var. sylventris. Mill) APPLE EXTRACT TOOTHPASTE GEL PREPARATION WITH VARIATIONS OF CMC NA CONCENTRATION AS ANTIBACTERIAL AGAINST Streptococcus mutans ATCC 25175

Background. Dental caries can be experienced at various ages which is marked by the appearance of white spots on the tooth surface by S. mutans bacteria. Manalagi apples contain compounds that are effective as antibacterial, namely polyphenols, saponins, alkaloids, tannins and flavonoids. Gel toothpaste formula with variations in CMC-Na of F1(1.5%), F2 (2%), and F3 (2.5%).
 Purpose. The purpose of this study was to determine the effect of variations in CMC-Na concentration on the physical quality and antibacterial activity of S. mutans in the Manalagi apple extract gel toothpaste.
 Methods. In addition, apple simplicia was extracted using 70% ethanol. Manalagi apple extract 15% was formulated into 3 formulas F1, F2, and F3 with CMC-Na concentrations of 1.5, 2, and 2.5%, respectively. Toothpaste gel preparations were tested for physical quality for 21 days, stability test for 3 cycles and antibacterial activity test against S. mutans by disc diffusion method. The data were processed with Shapiro-wilk statistics, ANOVA and Krustal Wallis, followed by the Paired t test and Wilcoxon
 Results. The study showed that F1, F2, F3 affected the physical quality, among others, caused a decrease in the dispersion area but increased viscosity, pH and vice versa, and the Manalagi apple extract gel toothpaste with varying concentrations had antibacterial activity with inhibition zones 39 , respectively. 8; 39.6 and 37.3 mm, and the best formula was F1.
 Conclusion. Manalagi apple extract has activity in inhibiting the growth of S. Mutans bacteria with a strong to very strong category, statistically there is a significant difference between variations in the concentration of CMC-Na.

RELEASE OF APPLE EXTRACT AND PHENOLIC COMPOUNDS FROM FIVE SEMI-SOLID FORMULATIONS

The clinical effectiveness of the phenolic compounds from apple extract will depend not only on their activity potency but also on the achieve of their action targets, and to achieve local biological effect from designed topical formulations, active substances have to be released from it. The aim of our study was to perform release test in vitro of emulsion, emulgel, gel, ointment, and oleogel containing an apple extract and individual phenolic compounds solutions. The release of phenolic compounds from semi-solid forms was assessed by Franz-type diffusion cells. The phenolic compounds of apple extract, and pure individual phenolic compounds released from five different semi-solid formulations results arrange in the following order: oleogel< ointment< gel< emulgel< emulsion. The in vitro release test showed that after 6 hours analysis the highest amount (70.6%) of phenolic compounds was released from emulsion, the lowest contents 31.6% and 29.2% were released from ointment and oleogel, accordingly. We found, that chlorogenic acid was the predominant phenolic compound released from the five different semi-solid forms after 1 ̶ 6 hours. The physicochemical properties of the investigated semi-solid formulations might have influenced the content of phenolic compounds released.

Preventive Effects of Thinned Apple Extracts on TNF-α-Induced Intestinal Tight Junction Dysfunction in Caco-2 Cells through Myosin Light Chain Kinase Suppression.

Inflammatory bowel disease (IBD) is associated with intestinal epithelial barrier dysfunction and elevation of proinflammatory cytokines such as TNF-α. Tight junctions (TJ) control the paracellular barrier of the gut. Thinned apples are an indispensable horticultural agro-waste for apple cultivation, but are disposed by most farmers. This study aimed to elucidate the preventive effect of thinned apple extracts (TAE) on the intestinal epithelial barrier dysfunction induced by TNF-α treatment in Caco-2 cells. The differentiated Caco-2 monolayers were pre-treated with mature apple extract (MAE) and TAE for 1 h and then incubated with 100 ng/mL TNF-α for 24 h. The TJ integrity was estimated by measuring the value of transepithelial electrical resistance (TEER) and the flux of fluorescein isothiocyanate-dextran through paracellular transport. TAE had a better protective effect on the intestinal epithelial barrier than MAE did. Western blot results showed that TAE pre-retreatment elevated TJ protein levels such as claudin-1, -4, and -5. Moreover, TAE inhibited the interaction between zonula occludens proteins (ZO)-1 and occludin by reducing the tyrosine phosphorylation of ZO-1. The mechanisms underlying TAE-mediated attenuation of TNF-α-induced TJ disruption included suppression of myosin light chain kinase and NF-κB p65 protein levels. Therefore, thinned apples could be a sustainable ingredient for functional foods to prevent IBD.

Structural and Physicomechanical Properties of an Active Film Based on Potato Starch, Silver Nanoparticles, and Rose Apple (Syzygium samarangense) Extract

In the current research work, active films were made from potato starch (PS) and AgNP solution comprising of silver nanoparticles (AgNPs) and rose apple extract (RE) via the casting method at various concentrations. AgNP solution in the PS matrix significantly altered the physical properties such as opacity, water vapor permeability mechanical property, solubility, and swelling index of the films. The influence of AgNP solution on the properties of the films was deeply examined. The results found that the 15% AgNP solution films exhibited better physicochemical properties. The presence of AgNP solution in the PS matrix significantly improved the properties of active films which is evident from the results of FTIR and SEM. Results show that AgNPs and PS were uniformly mixed and formed continuous and homogenous films without bubbles and cracks. In addition, the AgNP solution in the films significantly improved the antibacterial activity against S. aureus than P. aeruginosa in the films.

The Effect of HPMC Concentration as a Gelling Agent on Color Stability of Copigmented Blush Gel Extract of Purple Sweet (Ipomoea Batatas (L.) Lam.)

Purple sweet potato contains anthocyanin pigments that can be used as natural dyes. However, the use of anthocyanins as dyes has less stable properties. Anthocyanin stability can be increased by the addition of copigments. Copigment that can be used is apple extract at pH 4.6. In this study, a blush was made. One of the blush dosage forms that can be made is gel. Blush gel can provide a cool feeling, easy to wash, soft, and not sticky on the skin. In the creation of gels, the gelling agent is one of the components that affect the characteristics of gel preparations. HPMC is a gelling agent used in the manufacture of blush gel preparations. This study aims to determine the effect of increasing the concentration of HPMC as a gelling agent on the color stability of the copigmented purple sweet potato extract blush gel. The preparations were made in 3 formulas with varying concentrations of gelling agent (F1) 1%, (F2) 1.5%, and (F3) 2%. The evaluation carried out included testing the physical characteristics of the gel, namely organoleptic test, pH test, dispersion test, phase separation test, color stability test, and preference test. The results showed that the evaluation of blush gel preparations met the requirements of pharmaceutical standards with the pH test value of blush gel preparations being 4.72 to 5.84 and the spreadability test value being 5.26 cm to 6.48 cm. The results of the color stability test on the three formulas showed that there were significant differences. The increase in the concentration of HPMC as a gelling agent on the color stability of the copigmented purple sweet potato extract blush gel was unstable during 28 days of storage.

Skin Care Product Rich in Antioxidants and Anti-Inflammatory Natural Compounds Reduces Itching and Inflammation in the Skin of Atopic Dermatitis Patients.

The atopic dermatitis (AD) complex pathogenesis mechanism reveals marked changes of certain signaling factors as well as some morphological alterations in the epidermis. Reduced resilience against environmental factors and oxidative stress often makes the treatment with corticosteroids or tacrolismus ointments indispensable. In view of the correlation between oxidative stress and AD pathological factors, antioxidants can be incorporated into AD management strategies. This study investigates a curly kale, apple and green tea-containing natural extract rich in antioxidants for its effects on signaling inflammatory molecules and skin barrier enhancement in human epidermal keratinocytes- (NHEKs) based cell assays. Furthermore, the skin penetration on porcine ears was measured ex vivo using Raman micro spectroscopy. Finally, in a double-blind half-side, placebo-controlled clinical study, the effects of a formulation containing this extract were analyzed for the influence of lesion severity, epidermal barrier function, and pruritus in mild to moderately AD patients. Summarizing our results: The extract reduces expression of inflammatory cytokines in keratinocytes and increases barrier-related molecules. The verum formulation with a very high antioxidant capacity used in AD patients with mild to moderate lesions reduces itching, local SCORAD, and improves barrier function and the hydration of skin lesions.

Physical and chemical characteristics of beef marinated by cashew apple extract

Marination is one of the methods that are often used in beef processing in an attempt to obtain high quality of beef. Cashew apple extract marinade (CAM) improves the microbiological characteristics of meat by inhibiting the growth of meat bacteria. The effect of CAM on other aspects such as physical (microbiological, tenderness, cooking loss, water holding capacity and pH) and chemical (moisture, fat and protein content) characteristics of meat have not been evaluated, which would be beneficial for the utilisation of agroindustry waste in the meat industry. In this study, the effect of CAM on the physical and chemical characteristics of beef, including microbiological characteristics, tenderness, cooking loss and water holding capacity, was evaluated. CAM (0%, 10%, 20% and 30%) was used during beef processing for 4 h at refrigeration temperature. Significant effects of CAM were observed on the physical and chemical characteristics of beef. CAM increased beef tenderness and reduced total bacteria, cooking loss, moisture, fat, and protein content. The optimum concentration of CAM for which significant changes were observed in the physical and chemical characteristics of beef was 20%. Thus, cashew apple can be utilised as a promising marinade agent in beef processing with the criteria of food for specific health use. This approach will help reduce cashew apple waste and is an eco-friendly approach.

Effects of Polyphenols on Glucose-Induced Metabolic Changes in Healthy Human Subjects and on Glucose Transporters.

Dietary polyphenols interact with glucose transporters in the small intestine and modulate glucose uptake after food or beverage consumption. This review assesses the transporter interaction in vitro and how this translates to an effect in healthy volunteers consuming glucose. As examples, the apple polyphenol phlorizin inhibits sodium-glucose linked transporter-1; in the intestinal lumen, it is converted to phloretin, a strong inhibitor of glucose transporter-2 (GLUT2), by the brush border digestive enzyme lactase. Consequently, an apple extract rich in phlorizin attenuates blood glucose and insulin in healthy volunteers after a glucose challenge. On the other hand, the olive phenolic, oleuropein, inhibits GLUT2, but the strength of the inhibition is not enough to modulate blood glucose after a glucose challenge in healthy volunteers. Multiple metabolic effects and oxidative stresses after glucose consumption include insulin, incretin hormones, fatty acids, amino acids, and protein markers. However, apart from acute postprandial effects on glucose, insulin, and some incretin hormones, very little is known about the acute effects of polyphenols on these glucose-induced secondary effects. In summary, attenuation of the effect of a glucose challenge in vivo is only observed when polyphenols are strong inhibitors of glucose transporters.

Efficient Extraction of Total Polyphenols from Apple and Investigation of Its SPF Properties.

The purpose of this study was to evaluate the sun protection factor (SPF) of cosmetic emulsions with the addition of hydroalcoholic apple extract. First, the total polyphenolic content, the antioxidant activity and SPF properties of the extracts obtained by sonication and refluxing were evaluated. The two extraction methods were improved using the central composite design. For cosmetic emulsion that contained a different concentration of apple extract (10–40%), a SPF value between 0.51 and 0.90 was obtained. The most efficient apple extract was obtained by reflux using 50% ethanol and a 60 min extraction time. The concentrated extract was incorporated in a cosmetic emulsion whose SPF maximum was 0.90. Accordingly, due to photoprotective properties, the apple extract can be a candidate for use in cosmetic formulations.

Field Response of Tephritid Fruit Flies (Diptera) to Fruit Juice of Some Botanicals and Implications for Bio-Rationale Pest Management in Sudan

Fruit flies belonging to the order Tephritidae are the most notorious pests that attack various fruit and vegetable species, causing severe economic losses. Guava orchards at Elfaki Hashim; North of Khartoum, (Khartoum State) and Elsawagi Elgenobia (Kassala State) in the middle and east of Sudan respectively were selected to assess the response of some Tephritids to water extracts (WE) of mango, guava, apple, cucumber, and ready-made juice of mango (Crystal®; Crystal Industrial Co. Ltd, Araak Group; crystal@araak.com) against Torula yeast (a standard fruit fly attractant) and water (control) baited in locally made traps. The trial was performed as Randomized Complete Block Design (RCBD) replicated 3 times for 5 consecutive weeks at each site. Highly significant differences were recorded between attractants and the interaction between attractants and times (weeks) on trapping different species of fruit flies. Five species of fruit flies; Ceratitis capitata, Bactrocera dorsalis, Zeugodacus cucurbitae, Ceratitis cosyra, and Ceratitis quinaria, were found to respond positively to all tested materials at both sites. The greatest number of C. capitata flies was caught by traps equipped with ready-made Crystal® mango juice at the Elfaki Hashim site. The mean number of B. dorsalis attracted to the WEs of mango and guava at Elsawagi Elgenobia site was statistically identical to that of the same species lured to Torula yeast, 83.7, 70.3, and 111.5 flies /trap/week, respectively. Attractants extracted from botanicals are cost-free and easily prepared by farmers for mass trapping of fruit flies. More studies regarding active ingredients, doses, and the number of traps utilized per area should be considered.

Unravelling the immunomodulatory role of apple phenolic rich extracts on human THP-1- derived macrophages using multiplatform metabolomics

Apples represent a significant source of dietary phenolic compounds with evidenced anti-inflammatory and immunomodulatory activities. Nevertheless, the effect of the whole apple matrix on human macrophages is unknown. In this context, our study attempts to evaluate the effect of apple-derived phenolic compounds-rich extracts (pulp, peel and leaf) on IL-1β production in THP-1-differentiated macrophages and derived metabolic alterations through untargeted metabolomics. Our results have showed that apple pulp treatment inhibited the release of the pro-inflammatory cytokine IL-1β induced by LPS in THP-1 macrophages by ELISA analysis. Metabolomics demonstrate that different proportions of phenolic compounds led to differential alterations in the metabolism of THP-1 macrophages. Indeed, apple extracts promoted alterations in lipid, carbohydrate, amino acid and vitamins as well as cofactors metabolism. Specifically, leaf extracts were characterized by alteration of galactose metabolism while the extracts derived from the fruit showed predominant alterations in lipids metabolism. All extracts mimicked the response observed under normal conditions in LPS-stimulated macrophages, inhibiting LPS response. Thus, the phenolic enriched extracts from apples will be a good source of natural compounds with a beneficial effect against inflammation, and they may be applied as a food supplement and/or functional ingredient for the treatment of inflammatory diseases.

Formulation of Gels and Emulgels with Malus domestica Borkh: Apple Extracts and Their Biopharmaceutical Evaluation In Vitro.

Phenolic compounds that estimate apple extracts with multifaceted biological effects are potentially valuable for protection against skin disorders. The purpose of our research was to formulate gels and emulgels containing a complex of phenolic compounds of apple extracts and to perform a biopharmaceutical evaluation of semi-solid pharmaceutical forms, determining their antioxidant activity in vitro. HPLC analyses of phenolic compounds were performed. The total amount of phenolic compounds found in the sample of apples from the ‘Paprastasis antaninis’ cultivar was 1455.5 ± 72.8 µg/g. The release of phenolics from gels and emulgels was assessed by Franz-type diffusion cells. The in vitro release test revealed that phenolic compounds were released from the gel (G1–G6) formulations (70.6–73.8%) compared to the amounts (77.2–83.9%) released from the emulgel (E1–E6) formulations. The largest amount (83.9%) of phenolic compounds was released from the E5 formulation, while the smallest amounts (70.6%) were released from the formulations G3 and G5. The antioxidant activity evaluated by the DPPH and FRAP methods observed in all gel (G1–G6) and emulgel (E1–E6) formulations after 6 h were the strongest, compared to the activities observed in the formulations after 2 or 4 h. Gels and emulgels, which are rich in apple extracts, have strong antioxidant properties and may be promising choices for the development of new, innovative pharmaceutical forms or cosmetics.

Hypoglycaemic and hypolipidemic effects of unripe apple extract in a murine diabetic model

The increasing incidence of diabetes is a global concern. Current treatment modalities involving lifestyle modifications and pharmacotherapy are not effective in 50% of patients. Unripe apples contain polyphenols which are known to have antidiabetic effects. To verify the effects of unripe apple extract (UAE), the antioxidant activity of UAE was confirmed using a cell-free DPPH assay; and nitric oxide production and cytotoxicity were investigated in RIN-m5F cells. Biochemical analysis, oral glucose tolerance test, and histological analyses of the pancreas and liver were also conducted. UAE treatment induced hypoglycaemic conditions in alloxan-induced RIN-m5F cells. Further, UAE treatment showed antidiabetic effects in db/db mice by reducing plasma glucose and fructosamine levels without affecting the food intake and body weight. Additionally, UAE improved glucose tolerance and lipid parameters, and restored the pancreatic tissue in db/db mice. These results demonstrated the antidiabetic effects of UAE on alloxan-treated pancreatic islet-derived cells and in db/db mice, and suggested that UAE can be potentially used as a medicine and dietary supplement or functional food in antidiabetic therapy.

Cashew Apple Extract: A Novel, Potential Green Reducing Agent for the Synthesis of Reduced Graphene Oxide

Abstract Reduced graphene oxide (rGO) is one of the promising materials which are used in energy storage devices, field effect resistors and as an additive in polymer composites for functional and structural applications. The reduction of graphene oxide (GO) to rGO is popular and attractive. Chemical reduction of GO is considered as one of the promising methods for the large-scale production of rGO. Herein, we report for the first time a sustainable, eco-friendly and facile approach for synthesizing rGO using cashew apple extract as a reducing agent. Reduction of GO by cashew apple extract was confirmed by the bathochromic shift in the UV-Visible spectrum. The extent of reduction by cashew apple extract at temperatures (80°C, 120°C, and 180°C) was determined by making use of X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Raman Spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR) and were morphologically confirmed using Transmission Electron Microscopy (TEM). Through this research work, we propose an environment friendly approach for the production of rGO.

Impact of Storage Controlled Atmosphere on the Apple Phenolic Acids, Flavonoids, and Anthocyanins and Antioxidant Activity In Vitro.

Apples are seasonal fruits, and it is important to prepare them adequately for storage and ensure proper storage conditions. In this study, we used ten different apple cultivars: ‘Alva’, ‘Auksis’, ‘Connell Red’, ‘Cortland’, ‘Ligol’, ‘Lodel’, ‘Noris’, ‘Rubin’, ‘Sampion’, and ‘Spartan’. We studied the qualitative and quantitative composition of phenolic compounds in the apple and apple extracts antioxidants activity before placing them in the controlled atmosphere chambers and again at the end of the experiment, eight months later. Different concentrations of O2, CO2, and N2, constant temperature, relative humidity, and removal of endogenous ethylene were continually maintained. HPLC analysis showed that the highest amount of 2265.7 ± 152.5 µg/g of chlorogenic acid was found in apple samples of the ‘Auksis’ cultivar stored under variant IV conditions. Different concentrations of gas in the controlled atmosphere chambers caused changes in antioxidant activity in whole apple and apple peel extracts. In our study, we found that the antioxidant activity of apple extracts varied between samples of different apple cultivars and depended on the composition of the controlled atmosphere. Determining the optimal storage conditions is beneficial to providing the consumers with apples that have a known and minimally altered chemical composition of phenolic compounds and the strongest antioxidant activity, which determine the use of apples in the healthy food chain.

Nonlinear optical studies of citrus fruit synthesized gold nanostructures

Citrus assisted eco-friendly green synthesis of gold (Au) nanostructures (NSs) were prepared by Vitis (grapes) and Ananas comosus (pine apple) extracts. The UV–Vis absorption resonance peaks of grapes and pineapple Au NSs were observed at λmax = 558 nm and 572 nm respectively, these indicate the formation of gold (Au) nanostructures. The extracts of the citrus fruits acted as stabilize and ionizing agents to make the NSs. Spherical and triangle shapes of the nanostructures were confirmed by transmission electron microscopy (TEM), it is revealed that the diameter size of the grapes and pineapple synthesized nanostructures were 15 nm, 54 nm and 18 nm, 42 nm respectively. The quenching of the photoluminescence of the Au nanostructures at different concentrations of Eu(TTFA)3 and Sm(TTFA)3 complexes were observed at the excitation of 350 nm. The quenching of the luminescence were observed at particular concentration. The nonlinear optical (NLO) properties of Au NSs were studied by Z-scan setup with 800 nm laser.

<i>Malus domestica</i> and <i>Solanum lycopersicum</i> Mixtures for the Synthesis of Graphene Silver and Zinc Oxide Nanocomposites

This study reports on the novel and simple green method involving the use of apple (Malus domestica) and tomato (Solanum lycopersicum) extracts in the synthesis of electroactive layers of silver nanoparticles|graphene oxide (AgNPs|GO) and zinc oxide nanoparticles|graphene oxide (ZnONPs|GO). The surface morphology of the green synthesized nanocomposites was studied using High-Resolution Transmission Electron Microscopy (HRTEM), High-Resolution Scanning Electron Microscopy (HRSEM) while the elemental analysis was studied using Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy and X-Ray diffraction (XRD) and their optical properties were further characterised using Ultraviolet Spectroscopy (UV-vis). The electrochemical studies of these nanocomposites were achieved using cyclic voltammetry (CV) where an increase in electron conductivity of the AgNPs|GO and ZnONPs|GO nanocomposite was observed. Comparatively, the silver nanoparticulate-based platforms were observed to have superior electrochemical properties as opposed to the zinc oxide-based platform. The observed electrochemical activities of the synthesized nanocomposites are a good indication of their suitability as electroactive platforms towards the development of electrochemical sensors. Electrochemical sensors are popular in the Electrochemistry field because they may be developed using different methods in order to suit their intended analytes. As such, the synthesis of a variety of electrochemical platforms provides researchers with a vast range of options to select from for the detection of analytes.

The Application of Pomegranate, Sugar Apple, and Eggplant Peel Extracts Suppresses Aspergillus flavus Growth and Aflatoxin B1 Biosynthesis Pathway

Even though the green revolution was a significant turning point in agriculture, it was also marked by the widespread use of chemical pesticides, which prompted severe concerns about their influence on human and environmental health. As a result, the demand for healthier and more environmentally friendly alternatives to control plant diseases and avoid food spoilage is intensifying. Among the proposed alternatives, food by-product extracts, especially from the most consumed fruits in Egypt, eggplant, sugar apple, and pomegranate peel wastes, were largely ignored. Hence, we chose them to evaluate their antifungal and antiaflatoxigenic activities against maize fungus, Aspergillus flavus. All the extracts exhibited multiple degrees of antifungal growth and aflatoxin B1 (AFB1) inhibitory activities (35.52% to 91.18%) in broth media. Additionally, diethyl ether 50% eggplant, ethanol 75% sugar apple, and diethyl ether 25% pomegranate extracts exhibited the highest AFB1 inhibition, of 96.11%, 94.85%, and 78.83%, respectively, after one month of treated-maize storage. At the same time, Topsin fungicide demonstrated an AFB1 inhibition ratio of 72.95%. The relative transcriptional levels of three structural and two regulatory genes, aflD, aflP, aflQ, aflR, and aflS, were downregulated compared to the infected control. The phenolic content (116.88 mg GAEs/g DW) was highest in the 25% diethyl ether pomegranate peel extract, while the antioxidant activity was highest in the 75% ethanol sugar apple extract (94.02 µg/mL). The most abundant active compounds were found in the GC-MS analysis of the fruit peel extracts: α-kaurene, α-fenchene, p-allylphenol, octadecanoic acid, 3,5-dihydroxy phenol, hexestrol, xanthinin, and linoleic acid. Finally, the three fruit peel waste extracts could be a prospective source of friendly ecological compounds that act as environmentally safer and more protective alternatives to inhibit AFB1 production in maize storage.

HRMS Characterization, Antioxidant and Cytotoxic Activities of Polyphenols in Malus domestica Cultivars from Costa Rica.

There is increasing interest in research into fruits as sources of secondary metabolites because of their potential bioactivities. In this study, the phenolic profiles of Malus domestica Anna and Jonagold cultivars from Costa Rica were determined by Ultra Performance Liquid Chromatography coupled with High Resolution Mass Spectrometry (HRMS) using a quadrupole-time-of-flight analyzer (UPLC-QTOF-ESI MS), on enriched-phenolic extracts from skins and flesh, obtained through Pressurized Liquid Extraction (PLE). In total, 48 different phenolic compounds were identified in the skin and flesh extracts, comprising 17 flavan-3-ols, 12 flavonoids, 4 chalcones, 1 glycosylated isoprenoid and 14 hydroxycinnamic acids and derivatives. Among extracts, the flesh of Jonagold exhibits a larger number of polyphenols and is especially rich in procyanidin trimers, tetramers and pentamers. Evaluating total phenolic content (TPC) and antioxidant activities using ORAC and DPPH procedures yields higher values for this extract (608.8 mg GAE/g extract; 14.80 mmol TE/g extract and IC50 = 3.96 µg/mL, respectively). In addition, cytotoxicity evaluated against SW620 colon cancer cell lines and AGS gastric cancer cell lines also delivered better effects for Jonagold flesh (IC50 = 62.4 and 60.0 µg/mL, respectively). In addition, a significant negative correlation (p < 0.05) was found between TPC and cytotoxicity values against SW620 and AGS adenocarcinoma (r = −0.908, and −0.902, respectively). Furthermore, a significant negative correlation (p < 0.05) was also found between the number of procyanidins and both antioxidant activities and cytotoxicity towards SW620 (r = −0.978) and AGS (r = −0.894) cell lines. These results align with Jonagold flesh exhibiting the highest abundance in procyanidin oligomers and yielding better cytotoxic and antioxidant results. In sum, our findings suggest the need for further studies on these Costa Rican apple extracts—and particularly on the extracts from Jonagold flesh—to increase the knowledge on their potential benefits for health.

Potential antioxidant effect of fruit peels for human use from northern Peru, compared by 5 different methods

The objective of the work was to determine the antioxidant potential in vitro of freeze-dried peel extracts of 20 fruits from the northern region of Peru through five tests (Folin-Ciocalteu, DPPH., ABTS+., FRAP and CUPRAC). According to multivariate statistical analyzes, five groups were found: (i.) peel extracts with the highest values of antioxidant capacity (AC) from custard apple, and star fruit; (ii.) rind extracts with high AC values from quince, sweet granadilla, guava, and black grape; (iii.) husk extracts with middle values of AC from passion fruit, and red mombin; (iv.) shell extracts with low AC values from tangerine, mandarine, and bitter orange; and, (v.) coating extracts with the lowest AC values from pawpaw, red pawpaw, muskmelon, dragon fruit, yellow and red indian figs, pear, apple, and green grape. To conclude, the fruit lyophilized-husk extracts of custard apple, star fruit, quince, sweet granadilla, guava, and black grape obtained the best AC.