The essential oil of Plectranthus amboinicus was extracted through steam distillation. Carvacrol, the major constituent of the oil, was identified and found to make up 60.003% of the oil. Carvacrol was isolated using column chromatography with silica gel (200-430 mesh) and hexane: ethyl acetate (100:1) eluent solvent mixture, resulting in a high purity of 98.32%. Antimicrobial activity testing revealed that both Staphylococcus aureus and MRSA strains were sensitive to carvacrol and Plectranthus amboinicus essential oil (20 mg/mL). The antibacterial zone diameters were measured to be 20.20 ± 0.36 mm and 17.33 ± 0.32 mm for S. aureus, and 24.23 ± 0.47 mm and 21.53 ± 0.55 mm for MRSA, respectively. The minimum inhibitory concentration (MIC) of carvacrol against S. aureus and MRSA was determined to be 0.5 mg/mL. The minimum bactericidal concentration (MBC) against S. aureus was 1 mg/mL, while it was 0.5 mg/mL against MRSA.