Event Abstract Back to Event Evaluation of antibacterial activity and chemical constituents of Melicope glabra (Blume) T. G. Hartley Hafizah Mohd Zaini1, Nur Kartinee Kassim1*, Zamirah Zainal Abidin2, Wan Noorina Jaafar2 and Nurul Nuha Kamal Bahrain2 1 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Malaysia 2 Department of Oral Clinical Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Malaysia Background Melicope glabra is an edible plant which belongs to the Rutaceae family. It has been traditionally used in treating several illnesses including stomach pain, high blood pressure and to revitalize the body. In the present study, the antibacterial activity of ethyl acetate extract from the bark of M. glabra was evaluated against two oral pathogenic bacteria, Enterococcus faecalis and Streptococcus mutans. E. faecalis and S. mutans are oral pathogens that have been recognized as an important etiological agent in human dental caries. It has been suggested that E. faecalis virulence may be related to resistance to intracanal medicaments while S. mutans is a potent initiator of caries that play an important role in caries formation. Methods A new flavonoid compound was isolated from the ethyl acetate extract of M. glabra stem bark and its structure was elucidated by performing various spectroscopic techniques (nuclear magnetic resonance, mass spectroscopy, infrared spectroscopy) and compared with related literature data. The antimicrobial activities were evaluated by TLC-bioautography and minimum inhibitory concentration (MIC). Results The ethyl acetate extract exhibited good inhibition zones by TLC-bioautography method while the MIC assay showed that the extract possessed significant antibacterial activity against E. faecalis and S. mutans with the values of 5.0 mg/mL and 0.625 mg/mL respectively. Assay guided isolation and purification of ethyl acetate extract successfully isolated a new flavonoid compound; 7-hydroxy-2-(4’-hydroxy-3’-methoxy-phenyl)-3, 5-dimethoxy-chromen-4-one. Conclusion Ethyl acetate extract from M. glabra stem bark exhibited significant antibacterial activity and therefore can serve as a potential plant-based antimicrobial agent. Acknowledgements 1. Department of Chemistry, Faculty of Sciences, Universiti Putra Malaysia. 2. Department of Oral Clinical Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia Kuala Lumpur. 3. Kementerian Pengajian Tinggi (Fundamental Research Grant Scheme; Vot: 5524954) Keywords: Melicope glabra, Bioassay guided isolation, Antimicrobial activity, Enterococcus faecalis, Streptococcus mutans Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Infectious diseases Citation: Mohd Zaini H, Kassim N, Zainal Abidin Z, Jaafar W and Kamal Bahrain N (2019). Evaluation of antibacterial activity and chemical constituents of Melicope glabra (Blume) T. G. Hartley. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00115 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Nur Kartinee Kassim, Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Malaysia, kartinee@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hafizah Mohd Zaini Nur Kartinee Kassim Zamirah Zainal Abidin Wan Noorina Jaafar Nurul Nuha Kamal Bahrain Google Hafizah Mohd Zaini Nur Kartinee Kassim Zamirah Zainal Abidin Wan Noorina Jaafar Nurul Nuha Kamal Bahrain Google Scholar Hafizah Mohd Zaini Nur Kartinee Kassim Zamirah Zainal Abidin Wan Noorina Jaafar Nurul Nuha Kamal Bahrain PubMed Hafizah Mohd Zaini Nur Kartinee Kassim Zamirah Zainal Abidin Wan Noorina Jaafar Nurul Nuha Kamal Bahrain Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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