Antidiabetic Activity Of Ethanolic Extract Research Articles

In Vitro Antidiabetic Activity of Ethanolic Extract from Justicia tranquebariensis: Insights from GC-MS Analysis and Computational Docking

In Vitro Antidiabetic Activity of Ethanolic Extract from Justicia tranquebariensis: Insights from GC-MS Analysis and Computational Docking

In Vitro α-Glucosidase, α-Amylase Inhibitory and Antioxidant Activities of Root Crude Extract and Solvent Fractions of Arbutus unedo L. (Ericaceae)

Roots of Arbutus unedo (Ericaceae) have been used traditionally to treat diabetes in some countries. Conducting more experiments is needed to support this plant's potential antidiabetic properties, though. Ethanolic extract yielded by cold maceration was subjected into fractionation to get hexane, chloroform, ethyl acetate, butanol, and aqueous fractions. Total phenolic contents and antioxidant capacity have been estimated utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric ion reducing antioxidant potential (FRAP) assay models, respectively. Antidiabetic activity of A. unedo extracts were evaluated using in vitro α-glucosidase, α-amylase inhibition activity. The ethanol extract had the highest antioxidant activity among used extracts. While the results of both α-glucosidase, α-amylase enzyme inhibition activity was detected to be dose-dependent, the strongest inhibition activity for α-glucosidase and α-amylase was shown by ethanol extract (526.65 and 522.66 µg mL-1 respectively) compared to the standard acarbose. Results demonstrated the beneficial effects of A. unedo extracts by showing antioxidant and antidiabetic activities.

GC-MS analysis and In-vitro anti-diabetic activity of ethanolic extract of theAtrocarpus Heterophylus (Unriped Jack Fruit)

GC-MS analysis and In-vitro anti-diabetic activity of ethanolic extract of theAtrocarpus Heterophylus (Unriped Jack Fruit)

Anti-diabetic Activity of Ethanolic Extract of Rosa Centifolia and Its Purified Fractions

Anti-diabetic Activity of Ethanolic Extract of Rosa Centifolia and Its Purified Fractions

ACUTE TOXICITY AND ANTIDIABETIC ACTIVITY OF PHYLLANTHUS NIRURI USING ZEBRA FISH

This study was designed to assess the acute toxicity and antidiabetic activity of Ethanolic extract of Phyllanthus niruri (EEPN). Several invivo studies have demonstrated that extracts of Phyllanthus niruri has significantly lowered the blood glucose levels and lowers the complications associated with Type 2 diabetes in rodents, but till date no studies have been reported using zebrafish model for antidiabetic activity using Phyllanthus niruri. In the present study zebra fish are divided into six groups and given doses of 6, 12, 25, 50, 100, 200, and 400 mg/L of EEPN for extraction. These fish are then examined at intervals of 6, 24, 48, and 72 hours.Thirty zebra fish were divided into control and treatment groups for the purpose of evaluating their anti-diabetic activity. For 30 days, high glucose diet was used to induce diabetes in all zebra fish. The test groups are treated with concentrations of the extracts 25mg/L & 50mg/L and standard with 20mM metformin. The blood glucose levels are estimated in alternative days for seven days and on seventh day fish were studied histopathologically. Acute toxicity study of EEPN does not show any mortality,morbidity, and behavioural changes even after 96 hrs at maximum concentration 400mg/L. The extract showed significant lowering of blood glucose levels on diabetic zebra models. Extensive study can be done on the extract to study the natural lead compounds from this plant which are responsible for antidiabetic activity.

Bellevalia edirnensis ÖZHATAY & MATHEW: Türkiye Endemik Bitkisinin Kallus Kültürünün Anti-Alzheimer ve Anti-diyabetik Aktivitesinin Araştırılması

Callus culture, an alternative technique for the production of secondary metabolites, is a plant tissue culture method. In this study, it was aimed to determine the anti-alzheimer and anti-diabetic activity of ethanol extracts of callus tissue obtained from the Edirne hyacinth (Bellevalia edirnensis ÖZHATAY & MATHEW), which is endemic and is in danger of extinction. Callus induction started by sterilization of the seeds in 20% bleach and 70% ethanol. Following, it was achieved by culturing in MS medium containing (Murashige ve Skoog) GA3 in 3 different concentrations in addition to the medium supplemented with 7 g/L agar and 30 g/L sucrose. Anti-Alzheimer activity of the obtained calluses were determined using the Ellman method which is a spectrophotometric method. Anti-diabetic activities were determined using the α-glucosidase inhibition method. According to the results obtained, it was found that the callus culture of Bellevalia edirnensis ÖZHATAY & MATHEW showed moderate Anti-Alzheimer and Anti-diabetic activity.

Pharmacological screening of antidiabetic activity of ethanolic extracts of justicia tranquebarensis on streptozocin induced rats.

Non-communicable diseases like diabetes mellitus are becoming major health problem these days. There is an increasing demand by patients to use the natural products (plants) with anti-diabetic activity due to the severe side effects associated with the use of insulin and oral anti-diabetic drugs. One such plant is Justicia tranquebareinsis which has been traditionally used for the treatment of diabetes mellitus. The aim of this study was, therefore, to evaluate hypoglycemic activity of ethanol extract of Justicia tranquebareinsis in streptozotocin-induced diabetic rats, respectively and to carry out acute toxicity test. Two doses (200 mg/kg and 400 mg/kg) of the ethanol extract of Justicia tranquebareinsis were administered to normal glucose loaded and diabetic rat to study blood glucose lowering effect. Ethanol extract of Justicia tranquebareinsis (200mg/kg and 400mg/kg, p.o.) showed significant tolerance (P < 0.05) to oral glucose load at 1 and 2 hrs after glucose load. The extract also produced significant (P < 0.05) blood glucose reduction at 4 hours after its administration in normoglycemic mice. The extract at 400mg/kg dose level produced significant (P < 0.05) reduction in blood glucose level at 2, 3 and 4 hours of treatment in streptozotocin (45 mg/kg) induced diabetic mice. Acute oral toxicity studies of ethanol extract of Justicia tranquebareinsis in rats showed no death or signs of toxicity at the dose of 2000 mg/kg indicating the safety nature of the extract. The results of the experiments showed that ethanol extract of Justicia tranquebareinsis has significant antihyperglycemic activity in streptozotocin induced diabetic rat and improvement in glucose tolerance and slight hypoglycemic activity in normal rat justifying the traditional claim for its use in diabetes.

Antidiabetic Studies of Cnestis ferruginea Ethanolic Root Extract in Wistar Rats

The need to develop new and affordable antidiabetic drugs with minimal side effects is a growing area of research interest. The medicinal plant Cnestis ferruginea DC has diverse pharmacological applications. Its leaves and roots are used for the treatment of diabetes by traditional healers. As a result, the antidiabetic activity of ethanolic extract of C. ferruginea (EECF) roots was evaluated in streptozotocin (STZ)-induced diabetic rats. The influence of EECF (200 and 400 mg/kg bw) was studied for three weeks, with glibenclamide as a positive control. The phytochemical screening of EEFC was also performed. Like glibenclamide, treatment with EECF caused a significant (p < 0.05) reduction in fasting blood glucose (FBG) in diabetic rats. A significant (p < 0.05) alteration in serum lipid profile parameters, kidney and liver function parameters, as well as oxidative stress markers in kidney and liver tissues, was observed in diabetic control rats relative to the normal control ones. But the treatment of diabetic rats with EEFC resulted in significant (p < 0.05) restoration of these parameters to a near-normal level. The phytochemical analysis of EEFC showed the presence of alkaloids, anthraquinones, cardiac glycosides, flavonoids, phlobatannins, saponins and tannins. Thus, the results offer proof to support the traditional use of C. ferruginea roots in the treatment of diabetes. The antidiabetic activity of EEFC may be due to its phytoconstituents.

Evaluation of the acute toxicity and antidiabetic activity of Coutoubea spicata “nicolau” ethanolic extract and ethyl acetate fraction

In order to understand antidiabetic potential and toxicity, this study aimed to evaluate the acute toxicity and antidiabetic activity of ethanolic extract and ethyl acetate fraction obtained from Coutoubea spicata “nicolau” shoots. Chemical constituents and acute toxicity were investigated. In alloxan-induced diabetic rats, extract and fraction were tested at dose of 100 mg/kg, p.o. Body weight gain, glucose, lipid profile and oxidative stress markers in serum and tissues were determined. In vitroantioxidant activity was performed. Swertiamarin, gentiopicrin, deoxyloganic acid, clovin and robinin, and their p-coumaric ester were identified. Extract and fraction were classified as safe (category 5). In diabetic rats, Coutoubea spicata reduced glycaemia, which was accompanied by body weight recovery gain and attenuation in oxidative stress markers. Fraction showed scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS) radicals and reducing power higher than that of the extract. Extract and fraction of Coutoubea spicata didn’t present significant toxicity and it can be investigated as a therapeutic alternative in diabetes.

Antidiabetic Activity of Ethanol Extract from Red Spinach Leaves (Amaranthus tricolor L.) on Mice

This study aims to determine the antidiabetic activity of ethanol extract of red spinach leaves (Amaranthus tricolor L.) on male mice. The study was conducted experimentally using 25 male mice divided into 5 treatment groups, namely the negative control group (CMC Na 0.5%), positive control (glibenclamide 0,65 mg/kg BW), and 3 others groups given ethanol extract of red spinach leaves at doses of 100, 200 and 400 mg/kg BW. Mice were induced with streptozotocin (STZ) dose of 100 mg/kgbw intraperitoneally. After 72 hours, diabetic mice were treated for 15 days orally and blood glucose levels were checked every 5 days.using a glucotest device. The results showed that Ethanol extract od Red Spinach Leaves (EERSL) doses of 100 and 400 mg/kg BW were not significantly different from the negative control CMC Na (p>0.05) in reducing blood glucose levels. EERSL doses 200 mg/kgbw and positive control glibenclamide significantly reduce blood glucose levels campared to negative control CMC Na (p<0.05). Ethanol extract of red spinach leaves has antidiabetic activity on male mice at a dose of 200 mg/kg BW, whose effect is not significantly different compared to the glibenclamide.

Antidiabetic Activity of Ethanolic Extract of Caesalpinia Sappan (Linn). On Alloxan Induced Diabetes Mellitus in Rats.

Antidiabetic Activity of Ethanolic Extract of Caesalpinia Sappan (Linn). On Alloxan Induced Diabetes Mellitus in Rats.

Antidiabetic activity of ethanol extract of leaves of flax plant (Linum usitatissimum L.)

The aim of the present study was to evaluate antidiabetic activity of ethanol extract of leaves of Flax plant (Linum usitatissimum) in streptozotocin (STZ) induced diabetic rats. Ethanol extract of leaves of Linum usitatissimum (EELL) or glibenclamide was administered to the rats orally daily for 28 days. Blood glucose levels at weekly interval and on 29th day, serum biochemical parameters were carried out to evaluate its antidiabetic effect in diabetic rats. Blood glucose, creatinine, BUN, AST and ALT levels were significantly increased in STZ induced diabetic rats as compared to vehicle control rats. After 28 days administration of EELL showed the significant decrease in above levels as compared to diabetic control rats. The study results suggest that the ethanol extract of leaves of Linum usitatissimum clearly demonstrated the antidiabetic activity in an experimental model of rats.

Antidiabetic activity of ethanol extract of leaves of flax plant (Linum usitatissimum L.)

Antidiabetic activity of ethanol extract of leaves of flax plant (Linum usitatissimum L.)

POTENTIAL OF KABAU LEAF ETHANOL EXTRACT (ARCHIDENDRON BUBALINUM (JACK.) I. C. NIELSEN) TO DECREASE OF BLOOD GLUCOSE LEVELS INDUCED ALLOXAN

Objective: This study describes the potential antidiabetic activity of ethanol extracts of Kabau leaves (Archidendron bubalinum)
 Methods: Extraction was done using the maceration method with 96% ethanol as solvent. The simplicia and extracts were characterized, screened for phytochemicals using Thin Layer Chromatography (TLC). Blood glucose levels were examined by GOD-PAP enzymatic method. Wistar rat was induced by alloxan (120 mg/kg BW i. p) to hyperglycemic condition, the dose variations of Kabau leaves extract i. e 250, 500, and 1000 mg/kg BW (p. o). The data was statistically tested using one-way ANOVA with a confidence level of (p<0.05).
 Results: The phytochemical screening showed the presence of alkaloids, flavonoids, phenolics, monoterpenes and sesquiterpenes, steroids, and triterpenoids, saponins. TLC showed that the extract contained spots (Rf 0.45) which are suspected to be flavone glycosides, biflavonyls, and unusually substituted flavones. Phenolic compounds (Rf 0.225; 0.25; 0.325 and 0.45) were characterized by a color change to blackish green after being sprayed with FeCl3. Saponin glycoside compounds (Rf 0.57) were characterized by the presence of purple spots after being sprayed with vanillin sulfate. Test animals in all test groups experienced hyperglycemia (>126 mg/dl) and a significant increase in blood glucose levels compared to the control group. MDA levels in test animals given a dose of 1000 mg/kg BW was 0.024±0.003.
 Conclusion: Ethanol extract of Kabau leaves can reduce blood glucose levels in hyperglycemic rats by 33% at a dose of 1000 mg/kg BW. The results of one-way ANOVA (p<0.05) and Measurement of MDA levels in test animals was 0.024±0.003.

Potent Antidiabetic Activity of Ethanolic Extract of Terminalia Arjuna Fruits on Streptozotocin-Induced Diabetic Wistar Albino Rats

Diabetes mellitus is the most common chronic endocrine and metabolic disorder, impacts the quality of life, and has become the leading cause of death worldwide. Now a day’s, herbal drugs are in great demand for the treatment of diabetes because of their traditional acceptability, lesser side effects, and adverse effects compared with allopathic medications. Terminalia arjuna fruits were selected for the study due to their abundant availability and based on ethanomedicinal folk claims. The fruits of Terminalia arjuna have been evaluated for their antidiabetic and antihyperlipidemic activities using streptozotocin-induced diabetic Wistar albino rats. In the study, two doses of ethanolic extract of Terminalia arjuna fruits (TAF) were selected and administered to normal rats for an oral glucose tolerance test. For an antidiabetic study in streptozotocin-induced diabetic rats, the effect of the extract was observed for 28 days for blood glucose alterations. All experimental animals were observed for weight changes, due to STZ-induced diabetes, and were found to be significantly reverted to normal in all animals except the diabetic control group. After the completion of the experimental period, the collected serum samples were subjected to various biochemical parameters studied, including a lipid profile, glycosylated hemoglobin (HbA1c), urea, and creatinine level. An antioxidant study was performed using collected tissues, followed by histopathological studies of the pancreas. The research study revealed that the TAF extract has significant antidiabetic activity and the potential to revert the altered lipid profile and increase HbA1c to normal. TAF extract significantly normalized the urea, creatinine, superoxide dismutase, catalase, and glutathione peroxidase levels. Thus, the study concluded that TAF extract exhibits significant (p>0.05) antidiabetic activity in STZ-induced diabetic rats.

In vitro antioxidant and antidiabetic activity of ethanolic extract of Prosopis species growing in Rajasthan, India

In vitro antioxidant and antidiabetic activity of ethanolic extract of Prosopis species growing in Rajasthan, India

Pharmacognostic Evaluation and Anti-Diabetic Activity of Ethanol Extract of Triumfetta cordifolia A. Rich (Tiliaceae) Leaves

Aim: The study was aimed at evaluating some pharmacognostic parameters and investigates the anti-diabetic activity of ethanol extract of Triumfetta cordifolia leaf
 Methods: The pharmacognostic profiling of Triumfetta cordifolia leaves was carried out using some standard pharmacognostic tools for crude drug standardization such as qualitative and quantitative microscopy, analytical evaluation and phytochemical screening. The plant material was extracted using cold maceration method in ethanol and fractionation was carried out using n-hexane, ethylacetate and butanol. The acute toxicity study was done following standard method. Diabetes in albino wistar rats was intraperitoneally induced using 120 mg/kg body weight of alloxan monohydrate. The diabetic rats where treated with 200 and 400 mg/kg body weight of the crude extract and 400mg/kg of each of the fractions. Glibenclamide was used as the standard drug (5 mg/kg) and diabetic rats without treatment as negative control. The procedure was also similarly performed using the non-diabetic rats. The administration of all treatments was done orally, once daily for 21 days and blood sera of the blood samples from rats across the groups were collected at the end of the treatment period and the concentrations of Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and Alanine aminotransferase (ALT) were evaluated.
 Results: The results of the qualitative microscopic evaluation of Triumfetta cordifolia leaf revealed paracytic stomata, unicellular trichomes, wavy wall epidermal cells and prismatic calcium oxalate. Quantitative microscopic study gave 23.67 ± 0.58 stomata number, 0.023 ± 0.00058 stomata index, 18.33 ± 1.53 Palisade ratio and 10.67 ± 0.58 vein-islet number while the analytical standard revealed 9.3 total ash, 3.5 water soluble ash and 1.34 acid insoluble ash. The phytochemical analysis revealed the presence of alkaloids, glycosides, Tannins, flavonoids steroids and terpenoids in the Triumfetta cordifolia leaves ethanol extract (TCEE). A significant reduction (P≤0.05) in fasted blood sugar level of diabetic rats was observed during treatment with Triumfetta cordifolia leaves extract and the blood sugar level lowering potential was comparable to the glibenclamide’s group. There was improvement of body weight in TCEE treated groups and ethylacetate fraction group. The Leaf extract of Triumfetta cordifolia showed a high significant (P≤0.05) ameliorating potential on liver’s degenerating hepatocytes evidenced by the comparable reduction in AST, ALT and ALP levels with the glibenclamide’s and diabetic’s groups.
 Conclusion: The overall results showed that Triumfetta cordifolia leaf possesses blood sugar lowering and liver hepatocytes regenerating potentials while the pharmacognostic profiling of the plant can serve as a reference and guide for future researchers.

Antihyperglycemic and hepatoprotective effects of Salvia tebesana Bunge in diabetic rats.

Background and purpose:Medicinal plants have been used to cure numerous diseases compared to orthodox medicines. The present study estimated the antidiabetic activity of ethanolic extract of Salvia tebesana Bunge in streptozotocin-induced diabetic rats.Experimental approach:In this study type 2 diabetes was induced in male rats by streptozotocin (65 mg/kg, i.p.). After diabetes induction, normal control groups were treated with distilled water, the positive control group received metformin (500 mg/kg), and the other groups were orally treated with ethanolic extracts of S. tebesana (100, 200, and 400 mg/kg) for 4 weeks. Changes in body weight and some biochemical parameters were determined.Findings / Results:The ethanolic extract of S. tebesana in all doses considerably declined serum glucose, total cholesterol, alanine aminotransferase, aspartate aminotransferase, and triglyceride compared with the diabetic control rats. Administration of ethanolic extract of S. tebesana reduced the serum of kidney and liver function factors and decreased the side effects on the function of these.Conclusion and implications:These results revealed the potential of S. tebesana for the cure of diabetes and its problems.

Antidiabetic Activity of Ethanolic Extract of Seed Kernel of Sapindus emarginatus in Rats

Hyperglycemia is indeed a dyslipidemia across a Globe. An International Diabetes Federation (IDF) has Predicted a certain 366 million more people have diabetes in 2011 and it has been raised up to 552 million by 2050. It approximated a certain 40 million people of hyperglycemia through India in 2007 and yet this may predicted to grow almost to 70 million affected by 2025. LD50 study of the ethanolic extract of S. emarginatus seed kernel (EESESK) has been done throughout Albino rats up to a daily dosage constrain of 2000 mg/kg orally (according to OECD guidelines No.425 of CPCSEA) 1/5th, 1/10th dosage as from maximum dose examined for LD50 study results were again for experimental test research to examine a impact of S. emarginatus for a anti diabetes action. Insulin inefficiency was found in two selected model for my study. Alloxan hydrate at such a mg dosage of 150 mg/kg i.p after 48 hrs, the EESESK seed kernel it at a dosage of 200 mg/kg but also 400 mg/kg p.o have been administered. Dexamethasone 10 mg/kg, i.p, once daily. And at the inter of 30 mins EESESK 200 mg/kg and 400 mg/kg p.o had been conducted in rats for a period of 11 and 21 days respectively. At the end of the experimental, serum biomarkers such as glycogen, high cholesterol, triglycerides, LDL, HDL, and VLDL were analyzed by using semi-auto analyzer. In both models EESESK at a dose of 400 mg/kg exhibited substantial impact. Alloxan hydrate increased serum biochemical markers like glucose, high cholesterol, triglyceides, LDL, VLDL levels but decreased HDL levels. Dexamethasone caused an increase in serum glucose, High cholesterol and reduces High density lipopretein-cholesterol levels. The present study indicates that S.emarginatus seed kernel useful for the management of diabetes mellitus. S.emarginatus pollen seed found important reducing of glycogen TC, TG, LDL, VLDL levels and increased level of High Density Lipoprotein through diabetic concept mice. A enhanced high density lipoprotein is also Cardioprotective activity. Therefore S.emarginatus seed kernel must have future role to forestall forming of atherosclerosis but also cardiovascular disease.
 Keywords: Antidiabetic activity, Ethanolic extract, Seed kernel and Rats

In vitro antioxidant and anti-diabetic analysis of Andrographis echioides and Andrographis paniculata ethanol extract.

It is of interest to analyse and compare the antioxidant and anti-diabetic activity of ethanolic extracts of Andrographis echioides and Andrographis paniculata. Andrographis echioides and Andrographis paniculata were collected from a local farm. In vitro antioxidant activity was assessed by the potential of Piperine, Lupeol, beta sitosterol; DPPH free radical scavenging assay was performed by Liyana Pathirana and Shahidi method. In vitro anti-diabetic activity was assessed by alpha amylase inhibitory activity and alpha glucosidase inhibitory activity. The data were analysed by one-way-ANOVA to check the statistical significance among the groups and considered at the levels of p<0.05. Both the ethanolic extracts of Andrographis echioides and Andrographis paniculata showed significant antioxidant and anti-diabetic potential in a dose-dependent manner (100-500µg) and can be used as potential antidiabetic agents. Similar to antioxidant potential, Andrographis paniculata exhibited an increased anti-diabetic potential compared to Andrographis echioides. Data shows that the ethanolic extracts of Andrographis echioides and Andrographis paniculata possessed antioxidant and anti-diabetic activity and hence our present findings conclude that both plants can be considered for the development of natural drugs for the management of diabetes.