Abstract
Callus culture, an alternative technique for the production of secondary metabolites, is a plant tissue culture method. In this study, it was aimed to determine the anti-alzheimer and anti-diabetic activity of ethanol extracts of callus tissue obtained from the Edirne hyacinth (Bellevalia edirnensis ÖZHATAY & MATHEW), which is endemic and is in danger of extinction. Callus induction started by sterilization of the seeds in 20% bleach and 70% ethanol. Following, it was achieved by culturing in MS medium containing (Murashige ve Skoog) GA3 in 3 different concentrations in addition to the medium supplemented with 7 g/L agar and 30 g/L sucrose. Anti-Alzheimer activity of the obtained calluses were determined using the Ellman method which is a spectrophotometric method. Anti-diabetic activities were determined using the α-glucosidase inhibition method. According to the results obtained, it was found that the callus culture of Bellevalia edirnensis ÖZHATAY & MATHEW showed moderate Anti-Alzheimer and Anti-diabetic activity.
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