Agents In Animals Research Articles

Parasitos gastrointestinais de suínos criados em uma granja em Nova Iguaçu, Rio de Janeiro

At the national level, there are several types of pig breeding that employ diversified technologies and management, depending on the financial conditions of each producer. This difference, evidenced in the productions, ends up reflecting the presence of infectious agents in animals, including infections determined by gastrointestinal parasites such as helminths and protozoa. Due to the lack of information and the importance of this topic, this study aimed to report, through a parasitological survey, the frequency of gastrointestinal parasites detected in pig raised on a farm located in the Nova Iguaçú city, Rio de Janeiro. For this purpose, 200 fecal samples from 40 pens were collected. Fecal samples were processed in a pool using the Lutz spontaneous sedimentation technique. In 100% of the samples, forms of parasites were detected, being mainly strongyles eggs (97.5%), followed by coccidia oocysts (87.5%), Balantioides coli cysts (75%), Strongyloides ransomi eggs (42.5%), Ascaris suum (25%) eggs and Trichuris suis (7.5%), with statistically significant differences between these frequencies. From the parasitological survey carried out, it was possible to identify a high frequency of parasites in the fecal samples of the animals, confirming that the pig farm had precarious conditions for sanitary management.

Recognizing the emotional state of human and virtual instructors

Students' learning from an instructional video could be affected by the instructor's emotional stance during a lesson. A first step in investigating this emotional design hypothesis is to determine whether students perceive the emotions displayed by an instructor during an instructional video. Building on Russell’s (1980, 2003) model of core affect and the media equation theory (Reeves & Nass, 1996) this study investigated how well participants were able to perceive different emotions portrayed by a human and virtual instructor (i.e., animated pedagogical agent) in a video lecture on statistics. Participants were shown short video clips of either a human instructor or virtual instructor displaying four different emotions: happy, content, bored, and frustrated. The participants were asked to rate how well each video clip displayed each of those four emotions. Participants were able to recognize each of the emotions displayed by the instructor but were much better at distinguishing between positive (happy and content) and negative (bored and frustrated) emotions than between active (happy and frustrated) and passive (content and bored) emotions. Furthermore, participants were able to recognize the emotions of the instructor for both the human instructor and the animated agent. However, emotions that involved higher activity (happy and frustrated) were more easily recognized in a human instructor than an animated agent. This research shows that learners are aware of the emotions being portrayed by an instructor, both human and animated agent, and establishes the first link in the chain between how the emotional tone displayed by an instructor affects learning outcomes.

Determination of Antibacterial Agents for Animals in Swine Muscles by Microbiological Screening and LC-MS/MS

The determination of antibacterial agents for animals in swine muscles was improved by microbiological screening and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. In the first instance, the residual drugs were extracted from the samples using the Na2EDTA-McIlvaine buffer (pH 6.0). Subsequently, the agents were purified utilizing a PLS-3 cartridge and extracted with acetonitrile. Considering the microbiological methods, the sensitivities of the investigated drugs were higher and the test plate conditions were improved using a new reference organism Geobacillus stearothermophilus. As a result, a microbiological screening approach able to detect 33 drugs at MRL was developed in Japan. Remarkable, no false positives were detected. Moreover, the same preparation method enabled rapid and reliable microbiological screening, resulting in efficient screening with no undeterminable results.

Immunostimulating effect of <i>Yersinia enterocolitica</i>-antigen conjugated gold nanoparticles

Yersinia enterocolitica is one of the most common causative agents of nosocomial infections and the causative agent of yersiniosis, an acute infectious disease of the gastrointestinal tract. Intestinal yersiniosis caused by Y. enterocolitica is a very pressing issue for clinical and veterinary medicine requiring use of sero- and immunodiagnostics to identify the causative agent in humans, animals and animal products. Gold nanoparticles represent one of the most popular antigen nanocarriers used for immunization and vaccination. The advantages of using gold nanoparticles as vaccine delivery vehicles are associated with their relatively small size promoting their tissue penetration, low toxicity and prolonged in vivo circulation, low cost, and reproducibility. Currently, the development of new gold nanoparticle-containing diagnostic tests and vaccines against viral, bacterial, parasitic infections, including bacteria of the genus Yersinia is underway. The aim of the study was to examine efficacy of 15 nm gold nanoparticles as an immunomodulator coupled to Y. enterocolitica-derived antigen for immunization and vaccination. Final conjugate construct was used to immunize laboratory animals. The methods of immunochemical analysis assessed sensitivity and specificity of the antibodies obtained. After that, protective effects were examined in animals vaccinated with antigen conjugates and comparison preparations. It was found that immunization with a Y. enterocolitica antigen-gold nanoparticle conjugate resulted in obtaining serum that specifically recognized yersiniosis ~35 and ~14 kDa proteins evaluated in Western blot analysis. It was shown that the specific sera cross-reacted with Y. pseudotuberculosis species, but not with intestinal group Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Proteus vulgaris, suggesting the generic specificity of the antibodies obtained. To determine the protective effect, animals were vaccinated with antigen-gold nanoparticle conjugates and comparison preparations. Two weeks after the last vaccination, mice were challenged intraperitoneally with pathogenic strain Y. enterocolitica culture. The groups immunized with antigen-gold nanoparticle conjugate had 70—80% survival rate, whereas all control mice died. It was shown that Yersinia-derived antigen-gold nanoparticle conjugates possessed higher immunomodulating activity than unconjugated antigen. The antibodies obtained can be used for effective yersiniosis immunodiagnostics. Thus, Y. enterocolitica antigen-gold nanoparticle conjugates may serve as a basis for creating preventive vaccines.

Novel Antimicrobial Peptide from Temporin L in The Treatment of Staphylococcus pseudintermedius and Malassezia pachydermatis in Polymicrobial Inter-Kingdom Infection.

Interkingdom polymicrobial diseases are caused by different microorganisms that colonize the same niche, as in the case of yeast-bacteria coinfections. The latter are difficult to treat due the absence of any common therapeutic target for their elimination, both in animals and humans. Staphylococcus pseudintermedius and Malassezia pachydermatis belong to distinct kingdoms. They can colonize the same skin district or apparatus being the causative agents of fastidious pet animals’ pathologies. Here we analysed the antimicrobial properties of a panel of 11 peptides, derived from temporin L, against Malassezia pachydermatis. Only peptide 8 showed the best mycocidal activity at 6.25 μM. Prolonged application of peptide 8 did not cause M. pachydermatis drug-resistance. Peptide 8 was also able to inhibit the growth of Staphylococcus pseudintermedius, regardless of methicillin resistance, at 1.56 μM for methicillin-susceptible S. pseudintermedius (MSSP) and 6.25 μM for methicillin-resistant S. pseudintermedius (MRSP). Of interest, peptide 8 increased the susceptibility of MRSP to oxacillin. Oxacillin MIC value reduction was of about eight times when used in combination with peptide 8. Finally, the compound affected the vitality of bacteria embedded in S. pseudintermedius biofilm. In conclusion, peptide 8 might represent a valid therapeutic alternative in the treatment of interkingdom polymicrobial infections, also in the presence of methicillin-resistant bacteria.

The ability of lactic acid bacteria strains to remove di‐n‐butyl phthalate in simulated food matrices

SummaryIn this study, DBP (Di‐n‐butyl phthalate) was used as an example to evaluate the plasticiser‐binding ability of lactic acid bacteria strains (LABs). Three strains of Lactobacillus plantarum 121, Leuconostoc mesenteroides DM12 and Lactobacillus acidophilus NCFM were selected from LABs to test their DBP binding ability in different simulated food matrices. The results indicated that starch, gelatinised starch and glucose could significantly promote the DBP binding efficiencies of LABs. Low‐concentration proteins (<5%) increased the binding rates. However, denatured protein had no significant effect on the DBP binding ability. On the contrary, amino acids reduced the binding rates. AFM detection results indicated that the cell morphology of LAB binding with DBP had no change after treated in gastrointestinal digestive simulation systems. Collectively, the LABs could be employed as a biological detoxification agent for human and animals by eliminating plasticiser from foods, feeds and digestive tract in the future.

Multimodal perception of prominence in spontaneous speech: A methodological proposal using mixed models and AIC

Research on prominence perception has made use of animated agents and controlled speech in experimental settings, but these methodologies have disregarded some aspects of the acoustic and visual correlates of prominence. To overcome these limitations we propose a new methodological approach using spontaneous speech data. For this, we created a small database with extracts from a television talent show and neutralised the prominence-lending properties of the acoustic cues of prominence in the speech signal. In our pilot study twelve naïve listeners marked words for binary prominence (prominent vs. non-prominent) in two modalities, i.e. audio-only and audiovisual, under three conditions involving neutralisation of (a) fundamental frequency, (b) intensity, and (c) both fundamental frequency and intensity. Additionally, the marks of two trained listeners served as control condition. Different generalised linear mixed models were estimated and compared using the Akaike Information Criterion (AIC). The most parsimonious model was then examined using traditional null-hypothesis testing in order to provisionally establish the effects of our independent variables on prominence marking. We argue that spontaneous speech can be successfully applied to the study of the multimodal perception of prominence.

Acute reproductive toxicology after intratesticular injection of silver nanoparticles (AgNPs) in Wistar rats

This study aimed to evaluate the effects of an intratesticular injection of silver nanoparticles (AgNPs) on reproductive parameters and health of rats, and to evaluate the AgNPs biodistribution in order to develop a nanotechnological contraceptive agent for male animals. Treated animals received 220 μL of AgNPs solution (0.46 µg-Ag/ml) in each testicle and were euthanized: seven, 14, 28, and 56 days after injection. A significant decrease (p < 0.05) in the percentage of motile sperm in D7 (8.8%) was observed, comparing to the control (73.3%), D14 (86.0%), D28 (68.2%), and D56 (90.0%) groups. D7 group also presented a decrease (p < 0.05) in the percentage of normal spermatozoa. Additionally, D7 group showed an increase (p < 0.05) in abnormal midpiece and sperm head morphology compared to the Control group. Seminiferous tubules presented all germline cell types and spermatozoa for all groups. However, D7 group did not present spermatozoa in the epididymis, whereas some spermatozoa and cellular debris were visible in D14 and D28 groups. All animals presented hematological parameters, creatinine, and alanine aminotransferase values within the normal limits for Wistar rats. The percentage of silver found in the liver was always higher than in the other organs analyzed. A pioneering mathematical model is proposed, from which the half-life time of silver in the liver (17 days), spleen (23 days), lungs (30 days), and kidneys (35 days) was extracted. In conclusion, some acute and severe toxic effects were observed in sperm cells following intratesticular injection of AgNPs, although these effects were reversible. No adverse effects to general animal health were observed.

Speed Up, Size Down: How Animated Movement Speed in Product Videos Influences Size Assessment and Product Evaluation

Digital ads often display video content in which immobile products are presented as if they are moving spontaneously. Six studies demonstrate a speed-based scaling effect, such that consumers estimate the size of an immobile product to be smaller when it is animated to move faster in videos, due to the inverse size–speed association they have learned from the domain of animate agents (e.g., animals, humans). Supporting a cross-domain knowledge transfer model of learned size–speed association, this speed-based scaling effect is (1) reduced when consumers perceive a product’s movement pattern as less similar to animate agents’ movement patterns, (2) reversed when a positive size–speed association in the base domain of animate agents is made accessible, (3) attenuated for consumers who have more knowledge about the target product domain, and (4) mitigated when explicit product size information is highlighted. Furthermore, by decreasing assessed product size, fast animated movement speed can either positively or negatively influence willingness to pay, depending on consumers’ size preferences.

Do You Forgive Past Mistakes of Animated Agents? A Study of Instances of Assistance by Animated Agents

Many studies on human–computer interaction have demonstrated that the visual appearance of an agent or a robot significantly influences people’s perceptions and behaviors. Several studies on the appearance of agents/robots have concluded that consistency between expectations from an agent’s or a robot’s appearance and performances was an important factor to the continuous use of these agents/robots. This is because users would stop interacting with the agents/robots when predictions are not met by actual experiences. However, previous studies mainly focused on the consistency between an initial expectation and a performance of a single instance of a task. The influence of the orders of successes or failures for more than one instance of a task has not been examined in detail. Therefore, in this study, we investigate the order effects of how the timing of sufficient or insufficient results of animated agents affects user evaluation. This will lead to the contribution to fill the lack of studies regarding more than one task in the field of human–computer interaction and to realize the continuous use of agents/robots as long as possible and to avoid stopping to use the agents/robots owing to their successful design. We create a simulated retrieval website and conduct an experiment using retrieval assistant agents that show both sufficient and insufficient results for more than one instance of retrieval tasks. The experimental results demonstrated a recency effect wherein the users significantly revised their evaluations of the animated agents based on new information more than that based on initial evaluations. The investigation of the case of repeated instances of a task and the influence of successes or failures is important for designing intelligent agents that may show incomplete results in intelligent tasks. Furthermore, the result of this study will contribute to build strategies to design behaviors of agents/robots that have a high or low evaluation based on their appearance in advance to prevent users from stopping use of the agents/robots.

The livestock growth-promoter zeranol facilitates GLUT4 translocation in 3T3 L1 adipocytes

Zeranol is an approved but controversial growth-promoting agent for livestock in North America. It is a mycotoxin metabolite secreted by the Fusarium family fungi. The regulatory bodies in this region have established the acceptable daily intake and exposure below the level would not significantly increase the health risk for humans. However, their European counterparts have yet to establish an acceptable level and do not permit the use of this agent in farm animals. Given the growth-promoting ability of zeranol, its effect on energy metabolism was investigated in the current study. Our results indicated that zeranol could induce glucose transporter type 4 (GLUT4) expression in 3T3 L1 cells at 10 μM and initiate the translocation of the glucose transporter to the membrane as assayed by confocal microscopy. The translocation was likely triggered by the increase of GLUT4 and p-Akt. The insulin signal transduction pathway of glucose translocation was analyzed by Western blot analysis. Since no increase in the phosphorylated insulin receptor substrate in zeranol-treated cells was evidenced, the increased p-Akt and GLUT4 amount should be the mechanism dictating the GLUT4 translocation. In summary, this study showed that zeranol could perturb glucose metabolism in differentiated 3T3 L1 adipocytes. Determining the growth-promoting mechanism is crucial to uncover an accepted alternative to the general public.

Species-level drivers of mammalian ectoparasite faunas.

Traditionally, most studies have described the organization of host-parasite interaction networks by considering only few host groups at limited geographical extents. However, host-parasite relationships are merged within different taxonomic groups and factors shaping these interactions likely differ between host and parasite groups, making group-level differences important to better understand the ecological and evolutionary dynamics of these interactive communities. Here we used a dataset of 629 ectoparasite species and 251 species of terrestrial mammals, comprising 10 orders distributed across the Nearctic and Neotropical regions of Mexico to assess the species-level drivers of mammalian ectoparasite faunas. Specifically, we evaluated whether body weight, geographical range size and within-range mammal species richness (i.e. diversity field) predict mammal ectoparasite species richness (i.e. degree centrality) and their closeness centrality within the mammal-ectoparasite network. In addition, we also tested if the observed patterns differ among mammal orders and if taxonomic closely related host mammals could more likely share the same set of ectoparasites. We found that ectoparasite species richness of small mammals (mainly rodents) with large proportional range sizes was high compared to large-bodied mammals, whereas the diversity field of mammals had no predictive value (except for bats). We also observed that taxonomic proximity was a main determinant of the probability to share ectoparasite species. Specifically, the probability to share ectoparasites in congeneric species reached up to 90% and decreased exponentially as the taxonomic distance increased. Further, we also detected that some ectoparasites are generalists and capable to infect mammalian species across different orders and that rodents have a remarkable role in the network structure, being closely connected to many other taxa. Hence, because many rodent species have synanthropic habits they could act as undesired reservoirs of disease agents for humans and urban animals. Considering the reported worldwide phenomenon of the proliferation of rodents accompanying the demographic decrease or even local extinction of large-bodied mammal species, these organisms may already be an increasing health threat in many regions of the world.

New drugs for horses and production animals in 2019

In 2019, one novel pharmaceutical agent for horses or food-producing animals was released on the German market: An injection suspension for horses containing chondrogenic-induced equine allogeneic peripheral blood-derived mesenchymal stem cells as the active ingredient (ArtiCell® Forte) is now available. Two established veterinary active pharmaceutical ingredients became accessible for additional species: a salicylanilide anthelmintic oxyclozanide (Distocur®) for the treatment and control of fascioliasis in sheep as well as a type-I synthetic pyrethroid permethrin (Z-Itch®), which now has additionally been authorized for donkeys. Additionally, one veterinary drug with a new combination of active ingredients (toltrazuril + gleptoferron), 2 drugs (permethrin, omeprazole) in a new pharmaceutical formulation, one drug (lidocaine) with a novel route of administration, one drug (amprolium) with a higher content of the active ingredient, as well as one active substance (butylscopolaminium bromide) in mono-preparation have been launched on the market for horses and food-producing animals.

New drugs for small animals in 2019

In 2019, 2 novel pharmaceutical agents for small animals were released on the German market: The non-steroidal, non-cyclooxygenase inhibiting anti-inflammatory agent grapiprant (Galliprant®) of the piprant class as well as fluorescein (Ophtorescein®), a xanthen dye-based diagnostic agent for corneal defects. One substance was authorized for an additional species: The antidiabetic protamine zinc insulin of human origin (ProZinc®) is now additionally authorized for dogs. Additionally, one veterinary drug with a new combination of active ingredients (fluralaner + moxidectin), 5 agents in a novel pharmaceutical formulation (acepromazine, chloramphenicol, dexamethasone, metronidazole, torasemide), one drug (lidocaine) with a new route of administration, as well as one agent (apomorphine) in a new primary packaging with extended opening stability have been launched on the market for small animals.

Dietary polyphenols as antidiabetic agents: Advances and opportunities

AbstractDietary polyphenols have been widely investigated as antidiabetic agents in cell, animals, human study, and clinical trial. The number of publication (Indexed by Web of Science) on “polyphenols and diabetes” significantly increased since 2010. This review highlights the advances and opportunities of dietary polyphenols as antidiabetic agents. Dietary polyphenols prevent and manage Type 2 diabetes mellitus via the insulin‐dependent approaches, for instance, protection of pancreatic islet β‐cell, reduction of β‐cell apoptosis, promotion of β‐cell proliferation, attenuation of oxidative stress, activation of insulin signaling, and stimulation of pancreas to secrete insulin, as well as the insulin‐independent approaches including inhibition of glucose absorption, inhibition of digestive enzymes, regulation of intestinal microbiota, modification of inflammation response, and inhibition of the formation of advanced glycation end products. Moreover, dietary polyphenols ameliorate diabetic complications, such as vascular dysfunction, nephropathy, retinopathy, neuropathy, cardiomyopathy, coronary diseases, renal failure, and so on. The structure–activity relationship of polyphenols as antidiabetic agents is still not clear. The individual flavonoid or isoflavone has no therapeutic effect on diabetic patients, although the clinical data are very limited. Resveratrol, curcumin, and anthocyanins showed antidiabetic activity in human study. How hyperglycemia influences the bioavailability and bioactivity of dietary polyphenols is not well understood. An understanding of how diabetes alters the bioavailability and bioactivity of dietary polyphenols will lead to an improvement in their benefits and clinical outcomes.

Chemical composition and antibacterial activity of essential oils against pathogens often related to cattle endometritis.

Endometritis is a condition marked by inflammation of the endometrium that affects dairy cows from 21 days after parturition, causing damage to herd fertility and economic losses on farms. The use of active compounds obtained from plant sources has gained importance as disease treatment agents in farm animals due to the high resistance rates currently observed against traditional antibiotics commonly used. The study was carried out to examine the chemical composition and to investigate the antibacterial activity of rosemary, cinnamon, cloves, eucalyptus, lemon, oregano and thyme essential oils against the reference strain of Escherichia coli (ATCC 25922), Fusobacterium necrophorum (ATCC 25286), Trueperella pyogenes (ATCC 19411) and Staphylococcus aureus (ATCC 29213), considered as typical bacteria causing endometritis. The chemical composition of the seven essential oils were analyzed by GC-MS and their antibacterial activity was evaluated by the disc diffusion method. Thirty-six components were identified in total using GC-MS analyzes. The main compounds were cinnamaldehyde (86.5% for cinnamon essential oil), eugenol (85.7% for clove essential oil), 1,8-cineol (80% for eucalyptus and 47.8% rosemary essential oils), limonene (65.5% for lemon essential oil), carvacrol (72.1% for oregano essential oil) and thymol (48.8% for thyme essential oil). The disc diffusion assay revealed that cinnamon, clove, oregano, and thyme essential oils showed the best results compared to the other three essential oils, showing the largest zone of inhibition against all bacteria evaluated. These findings indicated that essential oils are a potential agent to be used as an alternative for bovine endometritis treatment.

Manual for reporting on zoonoses and zoonotic agents,within the framework of Directive 2003/99/EC, and on some other pathogenic microbiological agents for information derived from the year 2019

This reporting manual provides guidance to Member States (MSs) for reporting on zoonoses and zoonotic agents in animals, food and feed under the framework of Directive 2003/99/ECand of Commission Delegated Regulation (EU) 2018/772 and also on the reporting of other pathogenic microbiological agents in food. The objective of this manual is to harmonise and streamline reporting by MSs to ensure that the data collected are relevant and comparable for analysis at the European Union (EU) level. Thismanual covers all the zoonoses and zoonotic agents included under the current data collection system run by the European Food Safety Authority (EFSA). Detailed instructions are provided onthe reporting of data in tables and text in text forms. The instructions given relate to the description of the sampling and monitoring schemes applied by the MSs, as well as the monitoring results. Special reference is made to data elements which allow trend watching over time and the analysis of sources at the EU level. This manual is specifically aimed at guiding the reporting of information deriving from the year 2019.

Animals exhibit consistent individual differences in their movement: A case study on location trajectories of Japanese macaques

Researching individual recognition (IR) is essential to understand the life history and adaptive behavior of social animals. Investigation of possible IR clues may also help us build new hypotheses about how social animals distinguish between different individuals. This study investigates behavioral individuality in Japanese macaques (Macaca fuscata), focusing on one specific trait that is movement. Using a recently developed tracking system based on Bluetooth® Low Energy beacons, we collected three-dimensional (3D) location data from five Japanese macaques living in a group cage. A non-parametric, neural network-based analysis of the data revealed the existence of consistent individual differences in extremely limited aspects of the movement data (2-min trajectory of 3D location). Our results support the validity of multimodal approaches in studying IR, beyond the typical single-frame face recognition method, both for researchers and animal agents.

Development of luminescent serum for diagnostics of animal bacillus anthracix agent

The purpose of the study was to obtain luminescent serum based on highly purified anthracoid globulins to diagnose anthrax agents in animals. To date, there are plenty diagnostic agents that allow rapid and accurate diagnostics of infectious diseases of animals. One of them is the luminescent microscopy of the fluorescent antibody method, which is used as an express method and provides for diagnostics within 3-5 hours. Hyperimmune serum globulins prepared on two types of antigens – protective from strain 55 (VNIIVViM) and capsular from Lange-2 strain at five-fold scheme of introduction of these antigens – were used to make luminescent anthracoid serum. The luminescent serum made on the basis of highly purified anthracoid globulins has a coloring titer (specific activity) of 1:16. When examining the specificity of the obtained luminescent serum in smears from anthrax agent strains, clear fluorescence was observed with more intense luminescence along the periphery of microbial cells.

ФОРМИРОВАНИЕ СПЕЦИФИЧЕСКОЙ ИММУНОЛОГИЧЕСКОЙ АРЕАКТИВНОСТИ В ПЕРИОД БЕРЕМЕННОСТИ У СУПОРОСНЫХ СВИНОМАТОК

Changes of immunological reactivity to viral and bacterial antigens can cause in-creased susceptibility to infectious diseases. Different levels of this changes in newborn and adult animal organisms should be based on the fetus and newborn reactivity, first coming into contact with the antigen after birth, whereas the adult organism already has partial sensitization. Chronic vectors of pathological agents in animals and their in-fluence on the spread of infectious process is a persisting problem of modern veterinary medicine. The ability to use vaccination in newborns is limited by the presence of maternal antibodies that have immunosuppres-sive effects. High level of functional abilities of preg-nant organism is important in prevention of intrauterine infection. Infection in the prena-tal period of development affects fetal growth and development processes on the one hand, and on the other - isoimmuniza-tion of the maternal organism with fetal anti-gens occurs, accompanied by increased sen-sitivity of the organism with predominant manifestation of cellular phenomena, in the absence of increased synthesis of antibodies. Given the high importance of the functional reserves of the newborn organism, the intra-uterine development and completeness of the placental barrier are important. Detection of the transportation of infectious agent in inac-tive phase during pregnancy of different gestational period should be considered from the point of view of avidity and seropositivi-ty of pregnant animals. The high variety of clinical manifesta-tions of intrauterine infection requires the development of minimally invasive methods of antenatal and intranatal prediction, which allow at the stage of pregnancy and/or child-birth to assess the presence of an agent in a very small amount as the risk of the future development of infectious disease of the fetus and newborn, or complications of the early neonatal period.