Abstract
The purpose of the study was to obtain luminescent serum based on highly purified anthracoid globulins to diagnose anthrax agents in animals. To date, there are plenty diagnostic agents that allow rapid and accurate diagnostics of infectious diseases of animals. One of them is the luminescent microscopy of the fluorescent antibody method, which is used as an express method and provides for diagnostics within 3-5 hours. Hyperimmune serum globulins prepared on two types of antigens – protective from strain 55 (VNIIVViM) and capsular from Lange-2 strain at five-fold scheme of introduction of these antigens – were used to make luminescent anthracoid serum. The luminescent serum made on the basis of highly purified anthracoid globulins has a coloring titer (specific activity) of 1:16. When examining the specificity of the obtained luminescent serum in smears from anthrax agent strains, clear fluorescence was observed with more intense luminescence along the periphery of microbial cells.
Highlights
Introduction[1] At present, anthrax in only found in certain cases and outbreaks, as evidenced by its registration statistics in most countries of the world
Anthrax is an acute infectious disease dangerous to animals and humans
The modern production of diagnostic products is a single biotechnological system, which consists of successive stages and operations, the number and features of which depend on the type of product manufactured
Summary
[1] At present, anthrax in only found in certain cases and outbreaks, as evidenced by its registration statistics in most countries of the world. This infection has a global prevalence, not uniformly covering all continents and not registering in just a few island territories [2, 3]. The stability of anthracoid microbe in soil caused by the phenomenal ability of spores to persist for decades contributes to potentially dangerous anthrax areas leading to the development of epizootic and epidemic outbreaks of the disease. The causative agent exists in two forms: vegetative one in vivo and spore one in the environment, which causes the most important epizootiological and epidemiological characteristics of anthrax. The extreme survival of the spore forms of the pathogen in such an element of the environment as the soil creates favorable conditions for the formation of a natural foci
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