This paper aims to investigate the seismic behavior of single plate shear (SPS) connection between steel–concrete composite (SCC) beam and reinforced-concrete (RC) column. A total of four SCC beam to RC column composite joints were fabricated: one specimen was tested under monotonic loading, and three specimens were tested under cyclic loading. The failure modes, hysteretic and skeleton curves, strain distribution of anchor bars, RC slab and longitudinal reinforcement, ductility, energy dissipation and stiffness degradation were discussed, and the influence of longitudinal reinforcement ratio ρsl and the type of embedded parts on the seismic performance of joint were studied. The results indicated that the joint exhibited good seismic behavior. With the increase of ρsl, the initial stiffness, hogging moment and rotation capacities increased, while the ductility and energy dissipation capacity decreased slightly. The type of embedded parts had influence on the failure modes and initial stiffness, but had little effect on the moment and rotation capacities, ductility and energy dissipation capacity of joint. Finally, based on the Zhang’s model, considering the composite action of the floor slab and the contribution of additional bending moment, a calculation method was proposed to predict the ultimate sagging moment capacity of the joint, and the strength of the embedded parts of all specimens in this study and open literature were checked. The results showed that the Zhang’s model and the proposed model have a good prediction accuracy, and it is suggested that when designing the embedded parts of the joint under sagging moment, the cross-sectional area of anchor bars should be checked to avoid early failure.
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