Anand City Research Articles

Evaluation of Hand Written and Computerized Out-Patient Prescriptions in Urban Part of Central Gujarat.

Prescription order is an important therapeutic transaction between physician and patient. A good quality prescription is an extremely important factor for minimizing errors in dispensing medication and it should be adherent to guidelines for prescription writing for benefit of the patient. To evaluate frequency and type of prescription errors in outpatient prescriptions and find whether prescription writing abides with WHO standards of prescription writing. A cross-sectional observational study was conducted at Anand city. Allopathic private practitioners practising at Anand city of different specialities were included in study. Collection of prescriptions was started a month after the consent to minimize bias in prescription writing. The prescriptions were collected from local pharmacy stores of Anand city over a period of six months. Prescriptions were analysed for errors in standard information, according to WHO guide to good prescribing. Descriptive analysis was performed to estimate frequency of errors, data were expressed as numbers and percentage. Total 749 (549 handwritten and 200 computerised) prescriptions were collected. Abundant omission errors were identified in handwritten prescriptions e.g., OPD number was mentioned in 6.19%, patient's age was mentioned in 25.50%, gender in 17.30%, address in 9.29% and weight of patient mentioned in 11.29%, while in drug items only 2.97% drugs were prescribed by generic name. Route and Dosage form was mentioned in 77.35%-78.15%, dose mentioned in 47.25%, unit in 13.91%, regimens were mentioned in 72.93% while signa (direction for drug use) in 62.35%. Total 4384 errors out of 549 handwritten prescriptions and 501 errors out of 200 computerized prescriptions were found in clinicians and patient details. While in drug item details, total number of errors identified were 5015 and 621 in handwritten and computerized prescriptions respectively. As compared to handwritten prescriptions, computerized prescriptions appeared to be associated with relatively lower rates of error. Since out-patient prescription errors are abundant and often occur in handwritten prescriptions, prescribers need to adapt themselves to computerized prescription order entry in their daily practice.

English

A growing concern for public transit is its inability to shift passenger’s mode from private to public transport. The transit route network design (TRND) problem seeks a set of bus routes and schedules that is optimal in the sense that it maximizes the utility of an urban bus system for passengers while minimizing operator cost In this paper various data is collected to finding an optimal route length of VTCOS bus’s selected route, passenger capacity, total trip, boarding and alighting survey of passenger. TRANSCAD software provides useful tools such as road network links, shortest paths, O-D Matrices. This paper present some route of Anand city are very low passenger, so running empty buss and some route heavy passenger so increase in number of busses is advised. Our study area suggested route no. 2 & 7 is more reliable and suitable optimization route for the public transportation system.

Detection of Salmonella spp. from chevon, mutton and its environment in retail meat shops in Anand city (Gujarat), India.

Aim:The aim of this study was (i) To attempt isolation and identification of Salmonella species from samples. (ii) Serotyping of Salmonella isolates. (iii) Detection of virulence factor associated genes by polymerase chain reaction (PCR).Materials and Methods:A total of 284 samples comprised of chevon and mutton (112 samples each) as well as 60 samples (20 each of retail meat shops environment samples viz. Butchers’ hands, knives and log swabs) were collected from the retail meat shops in and around Anand City under aseptic precautions. Rappaport-vassiliadis soy bean meal broth and tetrathionate broth was used for the enrichment of all the samples and inoculation was done on brilliant green agar and xylose lysine deoxycholate agar. This was followed by the confirmation of isolates using biochemical tests. For the serotyping, isolates were sent to the National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh. Detection of virulence genes was performed by PCR technique using previously reported primer.Result:Of 284 meats and retail meat shops environment samples, 13 (4.58%) samples were found positive for Salmonella. It was interesting to know that incidence of Salmonella was more in mutton (6.25%) than chevon (3.57%). In case of meat shop environmental samples 1 (5.00%) sample observed positive for Salmonella separately among the butchers’ hands and knives swabs (Each of 20 samples) examined. Out of 13, eleven isolates detected as Salmonella Typhimurium, whereas only two isolates were detected as Salmonella Enteritidis. All Salmonella isolates possess invA and stn genes, whereas nine isolates had a presence of spvR gene while only five of the isolates revealed the presence of spvC gene as shown by in vitro detection of virulence genes by PCR.Conclusion:Therefore, might be suggested that the good hygiene practices and effective control measures should be taken to encourage clean meat production with prolonged shelf-life.

Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and anti-bio gram.

Aim:During the last decades, number of food poisoning cases due to Campylobacter occurred, immensely. After poultry, raw milk acts as a second main source of Campylobacter. Therefore, the present study was undertaken to detect the prevalence of Campylobacters in milk and milk products and to know the antibiotic sensitivity and virulence gene profile of Campylobacter spp. in Anand city, Gujarat, India.Material and Methods:A total of 240 samples (85 buffalo milk, 65 cow milk, 30 cheese, 30 ice-cream and 30 paneer) were collected from the different collection points in Anand city. The samples were processed by microbiological culture method, and presumptive isolates were further confirmed by genus and species-specific polymerase chain reaction using previously reported primer. The isolates were further subjected to antibiotic susceptibility assay and virulence gene detection.Result:Campylobacter species were detected in 7 (2.91%) raw milk samples whereas none of the milk product was positive. All the isolate identified were Campylobacter jejuni. Most of the isolates showed resistance against nalidixic acid, ciprofloxacin, and tetracyclin. All the isolates have three virulence genes cadF, cdtB and flgR whereas only one isolate was positive for iamA gene and 6 isolates were positive for fla gene.Conclusion:The presence of Campylobacter in raw milk indicates that raw milk consumption is hazardous for human being and proper pasteurization of milk and adaptation of hygienic condition will be necessary to protect the consumer from this zoonotic pathogen.

Biotyping of thermophilic Campylobacter spp. isolated from poultry in and around Anand city, Gujarat, India

Biotyping of thermophilic Campylobacter spp. isolated from poultry in and around Anand city, Gujarat, India

Isolation of pathogenic Escherichia coli from stool samples of diarrhoeal patients with history of raw milk consumption

Aim: To detect the occurrence of pathogenic Escherichia coli from stool samples of diarrhoeal patients with history of raw milk consumption and to determine the public health significance of isolates, especially their role in causing human diseases. Materials and Methods: A total of 100 stool samples from diarrhoeal patients, with history of raw milk consumption were collected from primary health centres in and around Anand city, under aseptic conditions and a total of 50 raw milk samples were collected from milk vendors, retail shops located in Anand city in sterilized sample bottles. MacConkey broth was used for the enrichment of all the samples and inoculation was done on MacConkey agar and EMB agar was used as the selective media. This was followed by the confirmation of isolates using biochemical tests. For the serotyping, E. coli isolates were sent to the National Salmonella and Escherichia Centre, Central Research Institute (CRI), Kasauli, Himachal Pradesh. Detection of virulence genes was performed using PCR technique. Results: During the present investigation, 26 (52%) E. coli isolates from 50 milk samples and 59 (59%) E. coli isolates from 100 stool samples were recovered. Out of 85 E. coli isolates sent for serotyping, 74 isolates could be typed which were further distributed into 13 different serogroups O2, O4, O8, O17, O22, O25, O29, O36, O45, O60, O90, O116 and O172, whereas 8 isolates were found untypable and 3 isolates were reported rough isolates. Of the 59 E. coli isolates from stool samples of diarrhoeal patients tested, 15 isolates (25.42%) were reported to be positive for stx genes, among that 6 (10.16%) were positive for stx1 gene, 9 (15.25%) isolates were positive for stx2 gene, while 3 isolates (5.08%) were positive for eaeA gene. In this study, 21 E. coli isolates were found to be Shiga toxin producing E. coli (STEC) while none of the isolates were positive for the serotype O157. Conclusions: Our present findings indicate that raw milk may act as a source of pathogenic E. coli and it may be responsible for the occurrence of diarrhoea and various other health-related complications in humans. We therefore recommend proper managemental practices and effective control measures for improved hygiene and sanitation.

Study on isolation, molecular detection of virulence gene and antibiotic sensitivity pattern of Escherichia coli isolated from milk and milk products

Aim: The study was undertaken to isolate pathogenic E. coli from milk and various milk products, detection of virulence gene using Polymerase chain reaction (PCR) and investigate their antibiotic sensitivity pattern. Materials and Methods: Altogether 250 milk and various milk products samples consisting of raw milk (50), cheese (50), ice-cream (50), mawa (50) and dahi (50) were collected from milk vendors, retail shops located in Anand city, under aseptic precautions. For the enrichment of the organism from the collected samples, MacConkey broth was used and inoculation was carried out on MacConkey agar and EMB agar. Later on, to confirm the isolates, various biochemical tests such as IMViC test, Urease test were performed. Evaluation of antibiotic sensitivity pattern of E. coli was assessed by disk diffusion method. Finally the E. coli isolates were screened for the presence of virulence associated genes by PCR . Results: The prevalence of E. coli was observed 32 % in the samples comprising of milk (52.00%), cheese (28.00%), icecream (20.00%), mawa (44.00%), and dahi (16.00%). Antibiotic sensitivity was recorded high for Co-trimoxazole (100%) followed by Gentamicin (96.73%), Trimithoprime (93.47%) and Doxycycline hydochloride (92.39%). Least sensitivity was recorded for Ampicillin (8.69%). In this study, out of 80 E. coli isolates, 25 isolates (31.25%) were positive for stx genes, of which 7 (8.75%) isolates were positive for stx1 gene only, while 12 (15.00%) isolates were positive for stx2 gene only and 5 (6.25%) isolates were positive for both stx1 and stx2, 7 isolates (8.75%) were positive for eaeA gene and all the isolate were negetive for rfb O157 gene. Conclusions: Current study supports the finding that raw milk and various milk products can be regarded as critical source of pathogenic E. coli This explains the need of strict monitoring and surveillance for effective measures of hygiene and sanitary practice during production of milk and various milk products.

Isolation and identification of Staphylococcus aureus from milk and milk products and their drug resistance patterns in Anand, Gujarat

Aim: The study was carried out with aim to isolate Staphylococcus aureus from milk and milk products (pedha and curd) and determine antibiogram pattern of S. aureus isolates. Materials and Methods: During 9 months duration of study a total of 160 milk and milk product samples (pedha and curd) were collected from different places in and around Anand city such as milk collection centre of Co-operative milk dairies, cattle farms, individual household, milk vendors and sweet shops. The samples were collected under aseptic precautions and were enriched in Peptone Water (PW) followed by direct plating on selective media viz. Baird-Parker Agar. The presumptive S. aureus isolates were identified by biochemical tests. Antibiogram pattern of S. aureus to antimicrobial agents were evaluated by disk diffusion method. Results: Analysis of result revealed that out of total 160 samples of milk (100) and milk products i.e. curd (30) and pedha (30) resulted in the isolation of 10 isolates (6.25 %) of S. aureus. In the present study S. aureus isolates were found variably resistant to the antibiotics tested. The S. aureus isolates showed highest sensitivity towards cephalothin (100.00 %), co-trimoxazole (100.00 %), cephalexin (100.00 %) and methicillin (100.00 %) followed by gentamicin (90.00 %), ciprofloxacin (80.00 %), oxacillin (70.00 %), streptomycin (60.00 %) and ampicillin (60.00 %). The pattern clearly indicated that the overall high percent of S. aureus isolates were resistant to Penicillin-G (100.00 %) followed by ampicillin (40.00 %), oxytetracycline and oxacillin (20.00 %) and streptomycin and gentamicin (10.00 %) Conclusions: Results clearly suggested a possibility of potential public health threat of S. aureus resulting from contamination of milk and milk products with pathogenic bacteria is mainly due to unhygienic processing, handling and unhygienic environment.

Zinc and Chromium Load in Road Dust, Suspended Particulate Matter and Foliar Dust Deposits of Anand City, Gujarat

Anand, the milk capital of India, is a developing city with increasing vehicles and developmental activities going on at a fast pace. This study attempts to investigate the zinc and chromium concentration in street dust, suspended particulate matter and in foliar dust deposits. Ten sampling locations were selected based on the traffic density on the roads and different anthropogenic activity. Sampling was carried out in the dry months of January to March 2011. The range of Zn and Cr was 16.82 - 108.29 ppm and 118 - 151.5 ppm in the street dust respectively. Zn concentration in Suspended particulate matter lies in the range of 12.41 to 86 ppm and Cr concentration between 75 to 130 ppm. The range of Cr in foliar deposited dust varied from 79.54 ppm to 31 ppm. Whereas, for Zn maximum concentration was in S10 which is 42.34 ppm and minimum was in site S9, 23.73 ppm. ANOVA single factor showed that at 0.05 level of significance site wise variation of zinc and chromium concentration in SPM, Street dust and foliar deposited dust was not significant signifying similar source of contamination. Which is further strengthened by the good positive correlation found between the Zn and Cr concentration of street dust, leaf deposited dust and SPM. The Contamination Factor in the sites where metal concentration was high was 1.24 in S10 and 1.06 in S5 for Zn. For chromium the value of CF was 1.77 in S10 and 1.67 in S5. These values indicate that street dust is moderately contaminated with respect to zinc and chromium.

Study on occurrence and antibiogram pattern of Escherichia coli from raw milk samples in Anand, Gujarat, India

Aim: The study was carried out with aim to isolate Escherichia coli from raw milk samples and determine antibiogram pattern of E. coli isolates. Materials and Methods: During 6 months duration of study a total of 100 raw milk samples were collected from different places in and around Anand city such as individual household, cattle farms, milk collection centres of Co-operative milk dairies and milk vendors. All raw milk samples were enriched in peptone water and inoculated on selective media and various biochemical tests were performed for confirmation of isolates. Antibiogram pattern of E.coli to antimicrobial agents was evaluated by disk diffusion method. E. coli isolates were sent to National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh for serotyping. Results: The result of present study revealed that out of 100 samples, 38 samples were found contaminated with E.coli. Antibiogram pattern revealed high resistance against ampicillin (100 %), whereas moderate resistance was observed for streptomycin (57.89 %), oxytetracycline (47.37 %) and amoxy-clav (42.11 %). Also lesser percentage of resistance was observed for co-trimoxazole (13.16 %) and chloramphenicol (5.26 %). Serotypes detected were O24 (7 isolates), followed by

Study of parasitic infections in non-human primates of Gujarat state, India

Aim: The study was conducted to provide baseline data on faecal parasites in groups of captive and free- living Non Human Primates of Gujarat state. Materials and Methods: Eighty two faecal samples from hanuman langur (Presbytis entellus) and fifteen samples from rhesus macaque were analysed from June, 2010 to March, 2011 using sedimentation technique as described by Georgi. Identification of parasitic ova was carried out as described by Soulsby (1982) and Wallach and Boever (1983). Results: Out of these 28 (34.14%) in hanuman langur and 6 (40.00%) in rhesus macaque, were found positive for the presence of total of six parasite species viz. Strongyloides spp., Trichuris spp., Entaemoeba histolytica spp., Ascaris spp., Entamoeba coli, Spirometra spp. Conclusion: Incidence of parasitic infection was 34.14% and 40.00% in Hanuman Langur and Resus macaque respectively. The exchange of disease is a concern for wildlife living Non Human Primates of Gujarat state. conservation both outside and inside the boundaries of parks and reserves. The presence of tourists, researchers Materials and Methods and park personnel has created a situation that may About 15 gram of fresh stool (faeces) was facilitate disease transmission between humans, collected in 10% formalin from each out of 82 livestock and wildlife (1, 2). Though, some data is Hanuman langur (Presbytis entellus) and 15 Rhesus available on parasitic infection in captive Non Human macaque (Macaque mulatta) during the period of Primates, little or no population data exist for parasitic June, 2010 to March, 2011. The sample collection was infections of free living Non Human Primates. The done without disturbing the animals. The sample paucity of data, combined with ever increasing human collection of free living animals was carried out from encroachment into previously pristine areas of the forest areas of Mount Abu and Ambaji, Anand city Neotropical forest, has necessitated this study with the and surrounding areas, Gandhinagar city and primary objective to provide baseline data on the surrounding areas, Panchmahal district forest areas presence of faecal parasites in groups of Non Human (Ratanmahal, Jambugoda and Pavagadh), Patan city Primates from free living and captive areas of Gujarat and surroun-ding areas whereas sample collection of state. In the Gujarat state mainly two species hanuman captive animals was carried out from Indroda park, langur and rhesus macaque of Non Human Primates Gandhinagar, Kamla Nehru Zoological park, Sayaji are exist more frequently with a population of 100865 Baug Zoo- Baroda as well as from Jiv Daya Charitable and 6115, respectively (3). Trust, Ahmedabad of the Gujarat state. The study of parasitic infection will be useful in When an identified individual defecated, faeces understanding the prevalence of parasites as well as to was collected immediately and placed in a polythene control morbidity and mortality in captive and free container containing formalin as fixative. The samples living Non Human Primates of the state.

Identification and Antibiogram of Microbes Associated with Bovine Mastitis

An investigation of Mastitis in cattle was carried out in Anand city and in nearby villages of Gujarat state using California Mastitis Test (CMT) kit. The prevalence of clinical and subclinical mastitis was found to be 5.5% and 15.75%, respectively. Staphylococcus aureus was identified through strain specific polymerase chain reaction; the remaining isolates identified on the basis of molecular analysis by 16S rDNA sequencing and phylogenetic analysis were Staphylococcus species, B. pumilus, Staphylococcus chromogenes, Bacillus species, and Pseudomonas species. In vitro antimicrobial susceptibility pattern of all the isolates was checked against 13 different antibiotics using the agar disc diffusion method. Highest bacterial resistance was observed with penicillin G and oxacillin antibiotics. It was also observed that the patterns of bacterial resistance have not changed in India over the years. The data supports the decrease in the incidence of mastitis but the rate of decrease is minimal. More effective control strategies are required.

Incidence of microalbuminuria in tobacco chewers.

It is well established that smoking increases the risk for cardiovascular disease. From the studies in diabetic subjects it has been shown that smoking induces microalbuminuria and accelerates the progression to end stage renal disease. Little is known whether smoking is also related to microalbuminuria and renal end organ damage in non diabetic subjects. The hypothesis which was put forward that tobacco chewing is related to microalbuminuria and renal functional changes in non diabetic subjects. We therefore performed a population based study in the Anand city of Gujarat in which we studied the relation between tobacco chewing and urinary albumin excretion. Tobacco chewers had a higher urinary albumin excretion (Albumin excretion 373±13.9 mg/day: P<0.01) than those who do not consume tobacco. In conclusion tobacco chewing is associated with albuminuria.

Land Use/Land Cover Change Mapping In Mahi Canal Command Area, Gujarat, Using Multi-temporal Satellite Data

The temporal changes (1988-89 to 1997) in land-use/land cover were studied using multi-temporal satellite data in Mahi Right Bank Canal (MRBC) command area in Kheda district of Gujarat state. The canal command area is affected by waterlogging and salinity. The land-use/land cover change is maximum in a distributary (Lambhvel) situated in highly urbanised zone around Anand city, where built-up area increased from 205 ha to 868 ha. In Nadiad command area also there is an increase in urban area from 281 ha to 460 ha, causing a decrease in agricultural area. Waterlogging is significant in Pansora command area with 586 ha of waterlogged area in 1997. Water logging has also increased in commands of other distributaries. The salt affected area has increased in Chikhaliya command whereas it has decreased in Manej command.

食品成分の分析法の開発と基準化について 栄養成分を中心として

A study was conducted to know the microbiological and chemical quality of mango pulp (5 samples each of loose and tinned mango pulp) available at Anand city. The average chemical composition of the loose mango pulp showed total solids 20.38, proteins 0.58, fat 0.61, ash 0.31, carbohydrates 18.88% and acidity 0.26 as percent citric acid. The corresponding values for tinned mango pulp were 25.68, 0.63. 0.53. 0.33, 24.19 and 0.65, respectively. The preservative (benzoic acid) was detected in only one loose sample and the coal-tar dyes were absent in all the samples. Among various microbial counts carried out, the average values for standard plate count, coliform count, yeast, mold, acid producers and psychrotrophic counts for loose mango pulp samples were 7.51. 4.57, 5.21, 4.70. 6.76 and 6.38 log cfu/g, respectively. The tinned pulp indicated average standard plate count and psychrotrophic count of 62 and 58 cfu/g, respectively. Coliforms were absent in lg product in all tins. The average values for yeasts, molds and acid producers' counts were 2. 3 and 20 cfu/g respectively. Test for two pathogens viz., Staphylococcus aureus and Salmonella spp. in 25 g product indicated presence of staphylococci in all 10 samples. Ilowever, all the isolates were coagulase negative. On the other hand, though the packed tins showed absence of Salmonella spp., they were detected in 3 out of 5 loose samples.