Ammoniation Treatment Research Articles

In Vitro Nutrient Digestibility and Ruminal Fermentation Characteristics of Ammoniated and Fermented Treatment of Soaked and Unsoaked Cymbopogon nardus Waste

Cymbopogon nardus waste has potential as an ingredient of cattle diet. C. nardus waste requires feed processing to increase ruminal fermentation characteristics. This study has aimed to discover the effect of fermentation and ammoniation treatment of soaked and unsoaked C. nardus on in vitro ruminal fermentation characteristics and nutrient digestibility. Five experimental diets with four replications were evaluated in this study: Cymbopogon nardus (CNW) as control, unsoaked fermented CNW (FCNW1), soaked fermented CNW (FCNW2), unsoaked ammoniated CNW (ACNW1), and soaked ammoniated CNW (ACNW2). A randomized block design was used and followed by post hoc Tukey-HSD if there is a significant effect (P<0.05). The results showed that there was no significant effect (P>0.05) of soaked or unsoaked ammoniated and fermented CNW on in vitro dry matter digestibility (DMD) and organic digestibility (OMD). Meanwhile, there was significant effect (P<0.05) on in vitro digestibility of crude protein (CPD), ADF (ADFD), NDF (ADFD), cellulose (CLD), and hemicellulose (HCLD). There was also no significant effect (P>0.05) of soaked or unsoaked ammoniated and fermented CNW on in vitro ruminal fermentation characteristics (pH, NH3, and total VFA level). In conclusion, soaked ammoniated of C. nardus waste has potential as cattle diet because it has high value of in vitro digestibility of crude protein (56.81%), ADF (47.67%), NDF (48.68%), cellulose (47.19%), and hemicellulose (50.52%).

Supercritical Ammoniation-Enabled Interfacial Polarization for Function-Mode Transformation and Overall Optimization of Thin-Film Transistors.

Thin-film transistors (TFTs) have drawn widespread applications in the increasingly sophisticated display field. Despite the mature process of fabricating enhancement-mode TFTs, lack of facile methods to realize depletion-mode TFTs restrains the implementation of complementary-type circuits, which in turn leads to relatively high power. Here, the supercritical fluid technique is introduced to elaborately design and tune the interface, providing the opportunity for function-mode transformation of TFTs. By harnessing supercritical-assisted ammoniation (SCA) treatment, interfacial polarization induces negative shift of threshold voltage (from 0.2 to -9.8 V), which allows TFTs to remain normally on-state in the absence of complex capacitor-integrated circuits. This convenient technique, without an additional manufacturing process to achieve function-mode transformation, can thus enable the fabrication of comprehensive-mode TFTs under the same process. Furthermore, comprehensive optimizations in the mobility (increases from 2.08 to 17.12 cm2 V-1 s-1), leakage current (reduces from 1.33 × 10-11 to 2.22 × 10-12 A), hysteresis (reduces from 11.2 to 0.2 V), and on/off current ratio (increases from 9.65 × 104 to 7.98 × 106) are achieved simultaneously. Based on conjoint analysis of electrical and material characterization, a reaction model is established for a clearer understanding of the interfacial polarization process. Overall, this low-temperature SCA treatment offers an environmentally benign strategy to modulate the function mode of electronic devices via interfacial engineering and optimize device performance at the same time, exhibiting promise in promoting the implementation of complementary, low-power circuit.

A novel ammoniation treatment of barley as a strategy to optimize rumen pH, feed degradability and microbial protein synthesis in sheep.

BACKGROUNDMeeting the energy and nitrogen (N) requirements of high‐performing ruminants at the same time as avoiding digestive disturbances (i.e. rumen acidosis) is a key priority in ruminant nutrition. The present study evaluated the effect of a cereal ammoniation treatment, in which barley grains are combined with urea and enzymes that catalyze the conversion of urea to ammonia to optimize rumen function. Twelve rumen cannulated sheep were randomly divided into two groups and fed a diet containing 60% of ammoniated barley (AMM) or untreated barley supplemented with urea (CTL) to investigate the impact on rumen fermentation and feed utilization.RESULTSAMM had higher total N content and effective rumen degradable N than untreated barely. AMM sheep had a consistently higher rumen pH throughout the day (6.31 versus 6.03) and tended to have a lower post‐prandial ammonia peak and higher acetate molar proportion (+5.1%) than CTL sheep. The rumen environment in AMM sheep favored the colonization and utilization of agro‐industrial by‐products (i.e. orange pulp) by the rumen microbes leading to a higher feed degradability. AMM sheep also had higher total tract apparent N digestibility (+21.7%) and urinary excretion of purine derivatives (+34%), suggesting a higher N uptake and microbial protein synthesis than CTL sheep.CONCLUSIONThe inclusion of AMM in the diet of ruminants represents a valid strategy for maintaining rumen pH within a physiological range and improving N utilization by the rumen microbes, which could have positive effects on the health and productivity of animals in intensive production systems. These findings warrant further studies under conventional farm conditions. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

The multi-level ammoniation on the digestibility of palm press fiber.

Objective:This research aimed to study the multi-level ammoniation on the digestibility of palm press fiber (PPF) through in vitro methods.Materials and Methods:This research was determined using a complete randomized design of three ammoniation treatments on PPF with three replications: (1) untreated PPF (Con), (2) ammonia treatment 4% (A-4), and (3) ammonia multi-levels of 6%, 4%, and 2% (A-Mul). Results:The results showed a significant effect on the chemical composition of feed in the A-Mul treatment compared to Con (p < 0.05) and a non-significant effect when compared to the A-4 treatment. Overall, the content of crude protein, ether extract, and crude fiber in the A-Mul treatment increased. Except in the dry matter (DM), neutral detergent fiber and acid detergent fiber decreased. The results of the in vitro analysis showed an increase in digestibility of DM, Organic matter digestibility, N-NH3, and volatile fatty acids (VFA) in ammoniation treatment. N-NH3 and VFA showed non-significant differences between treatments A-4 and A-Mul (p > 0.05).Conclusion:Multi-level ammoniation has affected the loosening of the fiber fraction bonding in the PPF, thereby changing the value of the chemical composition and the digestibility of PPF. Multi-level ammonia can be used as an alternative to processing high-fiber feed.

The Impact of Ammoniation Treatment on the Chemical Composition and In Vitro Digestibility of Rice Straw in Chinese Holsteins.

Simple SummaryRice straw has many essential uses as a byproduct of agriculture. As a feed source, due to low digestibility, low crude protein and minerals contents, the pretreatment of rice straw is required before use in ruminant feeding. To enhance the nutritive value of rice straw, different methods are practiced. Among them, treatment with ammoniation might be effective regarding the rice straw intake, enhancement of straw digestibility and crude protein levels, which are essential for enhancing the productive ability of dairy cattle. In the current study, we experimentally proved the efficiency of ammoniation treatment to enhance the different feed value parameters (dry matter digestibility, neutral detergent fiber, crude protein, gas production, acetic acid, butyric acid, and total volatile fatty acid) of rice straw.The current study was conducted to explore the ammoniation treatment effects on the chemical composition and in vitro digestibility of rice straw in Chinese Holsteins. For this purpose, rice straw was stored in polyethylene bags (35 × 25 cm, 350 g per bag) including (i) no additives (RS); (ii) 5% urea (5U, dry matter (DM) basis); (iii) 9% corn steep liquor + 5% urea (9C5U, DM basis); (iv) 9C2.5U; and (v) 9C2.5U + 3% molasses (9C2.5U3M, DM basis). The air-dry matter of the mixture was kept at the same level at 55% for all treatments. Fifteen bags (5 treatments × 3 repeats) were prepared and stored at ambient temperature (25 ± 3 °C). The chemical composition and in vitro digestibility were measured at day 60 after storage. Our analysis revealed that all the four ammoniation treatments improved the in vitro DM and neutral detergent fiber (IVNDFD) digestibility. In addition, all the four ammoniation treatments significantly (P < 0.001) increased the levels of crude protein (CP), gas production (GP), acetic acid (AA), butyric acid (BA) and total volatile fatty acid (TVFA) contents of the rice straw and decreased the neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the rice straw compared to the control. Within four treated groups, the 9C5U treatment was most effective. Finally, we concluded that ammoniation treatments increased the nutritive value of rice straw. In addition the 9C5U treatment could be an effective ammoniation treatment for the better utilization of rice straw.

(The Interactive Effect between Fermentation with Trichoderma reesei on Ammoniation on Fiber Component of Sugar Palm Pulp

The purpose of this research was to examine the combination effect of fermentation time by Trichoderma reesei and ammoniation on fiber component of sugar palm pulp. The research used sugar palm pulp from Boyolali which was sun-dried. Sugar palm pulp fermentation with Trichoderma reesei requires nitrogen for microbial growth. Ammoniation process could loose lignocellulosic bonds and provides the nitrogen supply for Trichoderma reesei, therefore these treatments could optimize Trichoderma reesei in the fermentation process. Samples were fermented for 0, 3 and 6 days with 1,5% Trichoderma reesei. The research treatments were allocated according to a completely randomize design with pattern of 2x3 factorial. First factor was ammoniation treatment and second factor was variation of fermentation time. The parameters observedd were NDF, ADF, hemicellulose, cellulose and lignin. Research result showed that there is was no interactive effect between treatments on reducing levels of NDF and ADF, but interaction treatments decreased (P&lt;0.05) levels of, cellulose and lignin. In conclusion, the interactive effet between fermentation time with Trichoderma reesei and ammoniation reduce levels of hemicellulose, cellulose and lignin in sugar palm pulp.

PENGARUH PERLAKUAN FERMENTASI DAN AMONIASI KULIT SINGKONG TERHADAP NILAI KECERNAAN BAHAN KERING DAN BAHAN ORGANIK RANSUM PADA DOMBA JANTAN

This research aimed to determine the effect of fermentation and ammoniation treatment of cassava peels on ration on dry matter digestibility (DMD) and organic matter digestibility (OMD) in male sheeps. This research was conducted in May--June 2019 in the cage unit and the Laboratory of Nutrition and Animal Feed, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. The experimental design applied was a completely randomized design (CRD) with 3 treatments and 3 replications. The experimental unit used were 9 sheeps. The treatments given were ration+15% cassava peels without treatment (R0), ration+15% fermented cassava peels (R1), and ration+15% ammoniated cassava peels (R2). The results showed that the use of cassava peel fermentation and ammoniation treatment had a significant effect (P&lt;0.05) on dry matter digestibility (DMD) and organic matter digestibility in male sheep. The average of total digestibility of dry matter and organic matter of ration showed an increase of digestibility. The treatment of fermentation and ammoniation gave the best effect when compared to that without processing.&#x0D; &#x0D; Keywords: Ammoniation, Fermentation, Cassava peels, Dry matter digestibility and organic matter digesbility, Sheep

PENGARUH PERLAKUAN AMONIASI DAN FERMENTASI KULIT SINGKONG TERHADAP NILAI KECERNAAN PROTEIN KASAR DAN SERAT KASAR PADA DOMBA EKOR TIPIS JANTAN

This study aimed to determine the effect of ammoniation and fermentation of cassava peel on the digestibility of crude protein and crude fiber and the best effect of ammoniation or fermentation on the digestibility of crude protein and crude fiber in thin-tail sheep. This research was conducted in April-August 2019 in the sheepfold of Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. The research used 9 male thin-tail sheep, feedstuff, and sheepfold with 9 pens and feed bunk. This study used a Completely Randomized Design (CRD) consisted of 3 replications and 3 treatments. The treatments given were R0 (basal ration + 15% cassava peel), R1 (basal ration + 15% fermented cassava peel) and R2 (basal ration + 15% ammoniated cassava peel). Data were analyzed with analysis of variance and continued with Least Significant Difference Test (LSD) at 5% and or 1% significant level. The results showed that the effect of ammoniation and fermentation treatment of cassava peel had no significant effect (P&gt; 0.05) on crude protein digestibility and crude fiber digestibility.&#x0D; &#x0D; Keywords: Thin-tail sheep, Crude protein, Crude fiber, Ammoniated and fermented cassava peel, digestibility

In vitro rumen fermentation characteristics of ammoniated stover of Samurai 2 sorghum fertilized with different level of Urea

The limitation stock of forages in dry season cause serious problem for ruminant productivity. Various efforts have been made to obtain an alternative feed. Samurai 2 has a high productivity, immune to leaf rust disease and midrange rot and suitable for animal feed. However, sorghum stover is a poor-quality fodder, low in protein and digestibility. It is needing to be processing to increase nutritive value by providing a source of protein and or non-protein and certain minerals through ammoniation. The effect of ammoniation treatments on a sorghum stover on in vitro feed fermentation and gas production on ruminal fluid was the main objective of this study. P12 treatment gave highest gas production than other treatment. Total gas production 48 hours, gas production from soluble fraction (a) and insoluble fraction (b) also total fraction (a+b) of the sorghum stover was significantly affected by treatments (P<0.05). Volatile Fatty acid (VFA), in vitro degradability, NH3, and biomass microbial rumen indicated no significant difference among treatments. In this study sorghum stover using basic fertilizer 90 kg/ha with ammoniation treatment has the best degradability on rumen fermentation characteristics in vitro of sorghum stover.

The effect of fermentation with different times of corn husk which has obtained ammoniation treatment in the production of VFA-NH3 by in vitro digestibility

The objective of the research is to examine the effect of the difference time of fermentation process using Aspergilus niger to corn husk that has been ammoniated on fermentability parameter in vitro which included the production of VFA and NH3. The aimed of this research was to determine the proper processing technique for corn husk in an effort to improve its quality as a complete feed ingredient for beef cattle. The experimental design used was completely randomized design with 3 treatment (different time of fermentation : 0; 1 and 2 weeks) and 4 replication. Corn husk which has been ammoniated by 6% ammonia with the temperature of 60 °C for 3 days, added 5% of Aspergilus niger as starter, than fermentation with different time. Parameter observed were fermentability parameter in vitro including the production of VFA and NH3. Data was analyzed using analysis of variance and if there was significant effect continued by Duncan’s test. The results indicate that the longer the fermentation process, the production of VFA and NH3 increased (P<0.05). The conclusion obtained is that the fermentation process using starter of A. niger 5% to 2 weeks can increase the production of VFA, NH3. The best production of VFA and NH3 occurs in 2 weeks, namely: 122.42 ± 4.35 and 5.99 ± 0.53 mm.

A Novel Capacitive Negative Electrode Material of Fe3N

A capacitive negative electrode material of Fe3N has been synthesized via a simple precursor ammoniation treatment. Electrochemical measure shows that the electrode exhibits a specific capacitance of 270[Formula: see text]F[Formula: see text]g[Formula: see text] at a current density of 0.5[Formula: see text]A[Formula: see text]g[Formula: see text] in 6[Formula: see text]M KOH, 66% of the initial value remains at a specific current density of 5[Formula: see text]A[Formula: see text]g[Formula: see text]. Capacitance retention after 5000 cycles is about 61.5% of the initial capacitance. The electrochemical performance is relatively great in comparison with the literature reports of some Fe-based compounds. Because of the low cost and simple preparation method of Fe3N, the material is promising in electrochemical capacitive energy storage applications.

Effect of urea treatment of cocoa pod on rumen fermentation characteristics in vitro

Indonesia is a third largest country in the world for cocoa production. A cocoa pod could be utilized as alternative feeds due to their sufficient quantity and availability throughout the year. On the other hand, low nutritional quality such as highly fibrous materials and low protein content usually characterized in agricultural and plantation by-products as it appears on cocoa pod. Ammoniation treatment using urea improve the nutritional quality of feedstuff. The objective of this study was to evaluate the effect of ammoniation treatments on a cocoa pod on in vitro feed fermentation and gas production on ruminal fluid. KA treatment gave highest gas production than other treatment. Total gas production during 48 hours of the cocoa pod was significantly affected by treatments (P<0.05). Total volatile acid (VFA), acetate (C2), propionate (C3), butyrate (C4) total VFA and A/P ratio indicated no significant difference among treatments. KA and KD treatments have tendency effect on IVDMD than control (KS) treatment. In this study ammoniation treatment using 5% of urea had a positive and effect on the best degradability and ruminal fermentation of cocoa pod.

Structure, phase transition and negative thermal expansion in ammoniated ZrW2O8

The local structural changes of ZrW2O8 after ammoniation treatment.

Synthesis and CO gas sensing properties of surface-nitridated Ga2O3 nanowires

Surface-nitrided Ga2O3 nanowire sensors showed responses of 160–363% at CO concentrations of 10–200 ppm at 150 °C. Our results showed that the sensitivity of Ga2O3 nanowire could be considerably enhanced by simple ammoniation treatment.

A study on feeding ammoniated and processed barley to feedlot steers

A total of 120 commercial crossbred steer calves (284±1.9 kg) were allocated in a 2×2 arrangement to two barley processing methods; whole (W) or rolled (R) barley and two ammoniation treatments; ammoniated (A) or non-ammoniated (N) barley. Steers were randomly allocated to twelve pens with 10 steers per pen and 3 pens (replications) per diet. The study was divided into two stages, growing (0–84 day) and finishing (85–196 day). Average daily gain (ADG) for the overall 196 day period was affected by processing ( p<0.01) averaging 1.10 and 1.20 kg day −1 for steers on W and R barley, respectively. Steers on the R barley gained 19.6% more than those on W barley during the growing stage and 0.8% more during the finishing stage. There was a trend towards an improvement ( p=0.06) in overall ADG by ammoniation. The ADG of steers was significantly higher ( p<0.01) during the finishing (1.22 kg day −1) than during the growing (1.06 kg day −1) stage. Feed efficiency was better with R than with W barley (6.8 vs. 7.7; p=0.02). Processing and ammoniation had no effect ( p>0.05) on carcass traits or grades.

Comparison of two Ammoniation Procedures to Reduce the Toxicity of Endophyte-Infected Tall Fescue Seed Fed to Rats

ABSTRACTTo determine the effect of extending the duration of ammonia (2% dry matter basis) treatment ti'om 1 to 5 wk on the toxicity of endophyte-infected tall fescue seed, 60 male Harlan Sprague-Dawley rats were randomly assigned to the following six treatments during a 28-d trial: endophyte-free (E-), endophyte-infected (E+), 1 wk ammoniated endophyte-fee (1AE-), I wk ammoniated endophyte-infected (1AE+), 5 wk ammoniated endophyte-free (5AE-), and 5 wk ammoniated endophyte-infected (5AE+) tall fescue seed The concentration of total pyrrolizidine alkaloids (N-acetyl and N-formyl loline) orE+ rescue was reduced from 4203 12g/g to 3009 and 2533 I-tg/g by the 1AE+ and 5AE+ treatments, respectively. Ergovaline was lowered from 3.77 to 1.57 12g/g by 1AE+ and eliminated by 5AE+. Endophyte-infected treatment groups had depressed (P<0.0001) dally feed intakes (DFI), daily weight gains (DWG), feed efficiencies (G/F), primary antibody responses, and T cell and B cell mitogenic responses than endophyte-free treatment groups. Ammoniation of endophyte-infected rescue seed improved DFI and DWG (P<0.0001) and G/F (P<0.05); however, there was no difference in performance criteria between the 1-wk and 5-wk ammoniation treatments. Endophyte-induced depressions in immune function were not alleviated by ammoniation.

Comparison of two Ammoniation Procedures to Reduce the Toxicity of Endophyte-Infected Tall Fescue Seed Fed to Rats

ABSTRACTTo determine the effect of extending the duration of ammonia (2% dry matter basis) treatment from 1 to 5 wk on the toxicity of endophyte-infected tall fescue seed, 60 male Harlan Sprague-Dawley rats were randomly assigned to the following six treatments during a 28-d trial: endophyte-free (E-), endophyte-infected (E+), 1 wk ammoniated endophyte-free (1AE-), 1 wk ammoniated endophyte-infected (1AE+), 5 wk ammoniated endophyte-free (5AE-), and 5 wk ammoniated endophyte-infected (5AE+) tall fescue seed. the concentration of total pyrrolizidine alkaloids (N-acetyl and N-formyl loline) of E+ fescue was reduced from 4203 äg/g to 3009 and 2533 ä;g/g by the 1AE+ and 5AE+ treatments, respectively. Ergovaline was lowered from 3.77 to 1.57 ä;g/g by 1AE+ and eliminated by 5AE+. Endophyte-infected treatment groups had depressed (P < 0.0001) daily feed intakes (DFI), daily weight gains (DWG), feed efficiencies (G/F), primary antibody responses, and T cell and B cell mitogenic responses than endophyte-free treatment groups. Ammoniation of endophyte-infected fescue seed improved DFI and DWG (P< 0.0001) and G/F (P< 0.05); however, there was no difference in performance criteria between the 1-wk and 5-wk ammoniation treatments. Endophyte-induced depressions in immune function were not alleviated by ammoniation.

Microbial degradation of mycotoxins

There is an important need to control aflatoxin formation under storage conditions and in the field. When aflatoxin is present in agricultural commodities procedures have been developed to detoxify such products. In particular, ammoniation treatment has been of particular success. However, the use of microorganisms to detoxify aflatoxin is now being actively pursued. This paper reviews the biological methods of detoxification giving particular attention to in vivo detoxification by the producer organism Aspergillus flavus.

EFFECTIVENESS OF ANHYDROUS AMMONIA AS A PRESERVATIVE FOR HIGH-MOISTURE HAY

Two experiments were conducted to determine the effectiveness of anhydrous ammonia as a preservative for high-moisture hays. In the first experiment, alfalfa hay, containing approximately 35% moisture was baled, stacked, sealed in polyethylene and treated with 1 and 2% (wt/wt) anhydrous ammonia. The plastic was removed at either 4 or 21 days after ammoniation and the bales were sampled following 4 mo in storage. The effects of ammoniation on heating, spoilage, chemical composition and in vitro organic matter digestibility (IVDOM) relative to untreated high- and low-moisture control stacks were determined. In the second experiment, ammonia was injected at the same rates into stacks of baled bromegrass hay containing approximately 30% moisture. The treated stacks were sealed for periods of 1 and 4 mo. In addition to the parameters measured in the first experiment, the bromegrass hays were fed to wethers to measure voluntary intake and digestibility in vivo. Injection of 2% anhydrous ammonia was completely effective in preventing heating and mold growth in the legume and grass hays during both the treatment period and following removal of the polyethylene cover. The 1% ammonia treatment reduced but did not prevent heat damage and mold growth. Ammoniation increased the crude protein content of all treated hays, the 2% application rate being more effective than 1%. Retention of the added N varied from 26.5 to 72% depending on application rate and duration of the treatment period. Applying 2% anhydrous ammonia to alfalfa hay prevented the decrease in IVDOM which occurred during storage in all other treatments and limited the increases in the proportion of NDF and ADF relative to the changes in these components which occurred in the high moisture control and 1% ammoniation treatments. Ammoniation of bromegrass hay did not affect voluntary intake (P &gt; 0.05). The treated hays were readily consumed by the wethers and no toxic or other deleterious effects were observed. Ammoniation of bromegrass hay increased in vivo digestibility of all components measured except protein. In general, the increases in digestibility were greater following application of 2% than 1% ammonia and for the 119 vs. the 29 day treatment period. Key words: Anhydrous ammonia, preservative, hay, sheep, digestibility

Effect of Ammoniation Treatment of Wheat Straw on in Vitro and in Vivo Digestibility

Effect of Ammoniation Treatment of Wheat Straw on in Vitro and in Vivo Digestibility