Alterations In Hematological Parameters Research Articles

Omalizumab does not lead to a distinct alteration in hematological parameters and complete blood count-derived inflammation biomarkers except for basophil count

Purpose Omalizumab is a monoclonal anti-IgE antibody used to treat patients with chronic spontaneous urticaria by decreasing free IgE levels. Omalizumab may have an anti-inflammatory effect by inhibiting T-cell activation and inducing eosinophil apoptosis. In this study, we evaluated the effect of omalizumab on hematological parameters and inflammation biomarkers in patients with chronic spontaneous urticaria. Methods Between July 2018 and November 2019, medical records of 60 patients (44 female, 16 male) with chronic spontaneous urticaria who were treated with omalizumab were reviewed retrospectively. Hematological parameters and inflammation biomarkers including the neutrophil/lymphocyte, monocyte/lymphocyte, platelet/lymphocyte and mean platelet volume/platelet count ratios were compared before and after 12 weeks of omalizumab treatment. Results The absolute count of basophils and percentage of basophils increased significantly after omalizumab treatment (p = 0.04, p = 0.004). The absolute count of eosinophils, percentage of eosinophils, neutrophil/lymphocyte, monocyte/lymphocyte, and mean platelet volume/platelet ratios decreased, while platelet/lymphocyte ratio increased after omalizumab treatment. Nevertheless, these changes were not statistically significant. Conclusions Increased basophil counts suggest that omalizumab has a crucial effect through basophils in chronic spontaneous urticaria. Further studies focussing on basophils may contribute to the literature both to elucidate the etiopathogenesis of urticaria and to improve novel treatment agents for the disease. On the other hand, our study revealed that omalizumab did not have a distinct effect on complete blood count-derived inflammation biomarkers and thus inflammation.

Dose dependent changes in oxidative stress, hematological variables, tissue pathology, and apoptosis following chronic sodium tungstate exposure in rats

Tungsten (W) toxicity has been identified as one of the major health concerns in the last decade. It has been identified in few hazardous waste sites and proposed for inclusion in the Environmental Protection Agency National Priorities List. The purpose of this study was to provide biochemical basis for understanding the tungstate induced interference in different organs and blood. Male Wistar rats were exposed to the concentration of 50, 100 and 200 ppm of tungstate in drinking water for 120 days. The hematological profiling was done after 15, 30 and 120 days. The biochemical estimation of oxidative stress parameters and histopathology of different organs were performed after 120 days. Significant reduction in the WBC and RBC counts, and hemoglobin levels was observed after exposure to 200 ppm of tungstate for 120 days. Significant alterations were observed in oxidative stress markers, being more pronounced in the liver and spleen. This was consistent with elevated number of apoptotic cells in spleen, and high tungstate concentration in blood, liver and spleen. Hence, we can conclude that the free radical induced oxidative stress which alters the antioxidant response are the possible mechanisms behind tungstate toxicity as we observed alterations in hematological parameters, histopathological observations, number of apoptotic cells, and bio-distribution of W. The study provides useful data for assessing the need to conduct health checkup in individuals living near hazardous waste sites or other sites associated with W exposure. Also, it provides information for assessing the potential health risks associated with tungstate toxicity.

Anti-tumor potential of cucurbitacin triterpenoids of Momordica dioica Roxb. fruit by EAC induced ascites tumor model

Momordica dioicaRoxb. (Cucurbitaceae) is commonly known as spiny gourd and traditionally used as astringent, febrifuge, antiseptic, anthelmintic, spermicidal and also used in bleeding piles, urinary infection and as a sedative. Studies indicate that it possesses antioxidant, hepatoprotective, antibacterial, anti-inflammatory, anti-lipid peroxidative, hypoglycaemic and analgesic properties. In this study, the anticancer efficacy of Cucurbitacins obtained from Momordica dioicaRoxb. (MDR) has been evaluated. Based on previous in-vitro studies performed, in-vivo studies were carried out on mice model. Ehrlich ascites carcinoma (EAC) cells were inoculated into swiss albino mice intraperitoneally to form a liquid tumor and then treated with oral administration of 50, 100, 200mg/kg. Evaluation parameters involved the mean survival time (MST), body weight, hematological parameters, Percentage increase in life span were measured in normal control, EAC control and Cucurbitacintreated groups (n = 6). Treatment with Cucurbitacins enriched fraction has shown anti-tumor effects against liquid tumor as indicated by a significant (P < 0.05) reduction in body weight. Interestingly, the enriched bio fraction restored the altered hematological parameters of tumor-bearing animals and significantly increased their life span. These data indicate the cytotoxic potential effects of MDRon tumor cells opening new opportunities for further studies on the anti-cancer effects of this agent.

Melatonin supplementation revives diabetic induced biochemical, histological and hematological impairments in rats

Diabetes is very common all over the world, but still not curable and controlled; it causes alteration in all over body. It needs serious concern for the scientific community to find out some control measures. The current work was planned to explore the possible defensive effect of melatonin against the diabetes induced changes in whole blood profile. For this study albino rats were treated with streptozotocin [(STZ) (15 mg/kg for 6 days)] to induce diabetes. Induction was confirmed by blood glucose and serum sugar assessment. Total 36 rats were randomly selected for the experimental purpose and were divided into two major groups. Major group-1 consisting eighteen (18) and were further sub-divided into three (3) different groups viz. group-I served as normal control, group-II served as melatonin treated, group-III served as glibenclamide treated. Major group-2 consisting eighteen (18) rats were given streptozotocin (STZ) injection (15 mg/kg) for 6 days. After confirmation of diabetes by measuring blood glucose level, animals having blood glucose level above 250 mg/dl) confirmed as diabetic. Eighteen (18) Diabetic rats were three subdivided into following sub-groups and were given different therapeutic treatments, Viz group-IV served as Diabetic control, group-V treated with melatonin, group-VI treated with glibenclamide, respectively. Diabetic rats demonstrated inflection in all hematological variables. Diabetic animals revealed considerable reduction in RBCs count, HB content and its associated indices (HCT, RDW, MCV, MCH and MCHC). Decrease in WBCs and its related indices (polymorphs and lymphocytes). Platelet count showed significant increase, but platelet distribution width (PDW %) was found decreased. However administration of melatonin restored all the alterations in hematological parameters. Therefore, it can be concluded that melatonin will be better therapeutic molecule to revive hematological alterations during diabetes.

Structural changes of serum albumin in response to oxidative stress caused byWalker-256 carcinosarcoma growth.

To assess oxidative stress and structural changes of the serum albumin in rats with transplanted Walker-256carcinosarcoma (W256) strains with varying sensitivity to doxorubicin (Dox). The study was performed on female Wistar rats with transplanted W256. On the 9th day after tumor cell transplantation an analysis of peripheral blood, oxidative stress parameters, and structural changes of serum albumin of experimental animals was performed. On the 9th day after W256transplantation a significant increase in the leukocyte counts was observed in the groups of animals with the Dox-resistant and parental (Dox-sensitive) W256tumors compared with the group of the intact animals: up to 14.24 ± 1.92•103/μl and 9.78 ± 1.03•103/μl, vs 8.92 ± 1.04•103/μl, respectively, due to the increase of granulocyte and monocyte counts. The number of lymphocytes was within the normal range. The level of hemoglobin and the erythrocyte counts were also within normal limits, but hematocrit in both groups of animals with tumors somewhat increased against the background of 1.2-fold elevation of the mean erythrocyte volume. In the group of rats with Dox-resistant W256, there was observed a decrease in the plateletcrit by almost 22% and thrombocyte counts- by 28%. Analysis of oxidative stress indices revealed a significant increase in the level of reactive oxygen species, 2-fold increase of malonic dialdehyde level and the degree of oxidative damage of blood plasma proteins, as well as a decrease in the activity of catalase in hemolysates (by 12-15%) in both groups of tumor-bearing rats. With the use of differential scanning calorimetry, UV and fluorescence spectroscopy we have revealed anomalous conformational changes of albumin caused by tumor development: structural rearrangements in the region of its first drug binding site located in the IIA domain, separation of globular parts of albumin molecule, and partial "opening" in a protein molecular three-domain structure resulting a loss of its thermal resistance. The development of transplanted Walker-256carcinosarcoma, especially its Dox-resistant variant, results in severe metabolic intoxication reflected in alteration of hematological parameters, and indices of oxidative stress, as well as architectonic changes of serum albumin.

Impact of a simulated marine heatwave in the hematological profile of a temperate shark (Scyliorhinus canicula)

• A simulated heatwave altered hematological parameters in temperate catsharks. • It increased leukocyte and thrombocyte counts, along with ventilation rates. • Erythrocyte counts and size ratio decreased in response to this treatment. As Earth’s temperature continues to rise, sudden warming events, designated as marine heatwaves (MHWs), are becoming more frequent and longer. This phenomenon is already shown to significantly impact marine ecosystems and respective fauna. While experimental acclimation to higher temperatures is known to affect predatory behavior, metabolism and overall fitness of sharks, the effects of short-term exposure to high temperatures on sharks’ physiology has yet to be investigated in a MHW context. Thus, the aim of our work was to study the impact of a category II MHW (Δ3 °C, 15 days) on: i) hematological parameters (total erythrocytes, leukocytes, thrombocytes, erythrocyte nuclear abnormalities (ENAs) counts, and nucleus to cytoplasmic ratio), ii) heart and spleen to body ratios, and iii) ventilation rates of juvenile catsharks ( Scyliorhinus canicula ). We found that MHW exposure led to significant changes in normal blood cell counts, by lowering erythrocyte counts and nucleus to cytoplasm ratio, and increasing leukocyte and thrombocyte counts. Moreover, ventilation rates increased consistently over the course of the MHW. However, there were no changes regarding the presence of ENA, as well as spleen and heart to body ratios. Our findings indicate limited capabilities for coping with sudden warming events, suggesting potential disruption in shark physiological homeostasis as the frequency, duration and intensity of MHWs are expected to be strengthened.

Haematological profile and its outcome in different species of malaria: a two year study

Background: Malaria is a major health problem in many parts of India and some parts of Andhra Pradesh, which is also one of the endemic areas for malaria. Several factors have been attributed to increased morbidity and mortality in malaria especially with altered hematological and coagulation parameters playing an important role. The aim of present study was to study the hematological and coagulation abnormalities that correspond to severity and the final outcome.Methods: The present study was carried out on 100 patients admitted during the period of November 2016 to October 2018 at Narayana Medical College and hospital, Nellore. All of these patients were confirmed by Peripheral Smear or MPQBC or Antigen Assay followed by detailed clinical history, physical examination and investigated with hematological and coagulation parameters. Then subsequently required routine and special investigation which was followed by monitoring the outcome of the patients with respect to morbidity and mortality.Results: Out of 100 patients 20 patients had severe anemia (Hb% <7 gm%) and most of them patients were falciparum and mixed infection cases. Thrombocytopenia was observed in 63% of the patients and severe thrombocytopenia (<50,000 mm3) was seen in 12% of the patients. PT and APTT were increased in 18% and 13% of the cases respectively. BT was increased in 5% of the cases. None of the patients expired in this study.Conclusions: severe anemia is a poor prognostic factor and has adverse outcome. Thrombocytopenia, increased PT, APTT does not have any correlation to mortality.

Effects of aqueous leaf extract of avocado (Persea americana) on total cholesterol, triacylglycerols, protein and haematological parameters in CCl4-intoxicated rats

BackgroundAvocado (Persea americana) is one of the plants widely used in ethnomedicine in Nigeria. The present study was aimed at investigating effects of aqueous Avocado (Persea americana) leaf extract on total cholesterol, triacylglycerols, protein and haematological parameters in carbon tetrachloride (CCl4)-intoxicated rats.MethodsWe evaluated the possible effects of pre-treatment with aqueous extract of Persea americana (AEPA) on protein, total cholesterol (T-CHOL), triacylglycerols (TAGs) and haematological parameters in Wistar male albino rats intoxicated with CCl4. Group 1 was the healthy control; group 2 rats were pre-treated with Reducdyn® (100 mg/kg/day) as a standard drug, groups 4 and 5 rats were pre-treated with AEPA at a dose of 100 mg/kg and 200 mg/kg per day respectively, the treatments were administered orally for 7 days. On the seventh day, rats in the treatment groups were injected with a fresh mixture of CCl4 and olive oil (3 ml/kg, 1:1; sc).ResultsPre-treatment of rats with AEPA resulted in marked increase (p < 0.05) in total protein and reduction in T-CHOL (19–34%) compared to CCl4 alone. Also, there was significant decrease (p < 0.05) in serum TAG concentration when rats were pre-treated with 100 mg and 200 mg kg− 1 b. wt. AEPA. Similarly, AEPA provoked (p < 0.05) a lowering of T-CHOL and TAG levels and an increase in liver protein concentration in the rats. Administration of AEPA at both concentrations restored (p < 0.05) WBC count and ameliorated neutropenia and lymphocytosis caused by CCl4 intoxication.ConclusionThese results suggest that AEPA could be protective against the development of fatty liver and might also be exhibiting the potential to prevent alterations in haematological parameters caused by CCl4 intoxication in rats.

Anti-arthritic evaluation of Eclipta alba in a murine model Freund’s adjuvant provoked arthritis

Eclipta alba (E.alba) is a medicinal plant with wide range of biological action encompassing antioxidant and anti-inflammatory. However, its anti arthritic activity is not reported till date. So we have evaluated the anti-arthritic property of E.alba methanolic extract in arthritis induced rats. The rats were made arthritic by single intradermal injection of complete freunds adjuvant (CFA) and E.alba (200 and 400mg/kg) were administered for 28 days. The assessment of arthritis was done by evaluating body weight, paw volume and alteration in hematological parameters (WBC, RBC, Hb and ESR). Further, to evaluate oxidative stress, malondialdehyde (MDA), a marker of lipid peroxidation and antioxidants (SOD, CAT, GPx and GSH) were measured. The arthritis induced rats showed significant decrease in body weight, elevated paw oedema, and changes in blood parameters. Treatment with E.alba significantly reduced the arthritic symptoms by its anti-inflammatory effect. Further, arthritic rats displayed elevated MDA and decreased antioxidant levels and treatment with E.alba inhibited the lipid peroxidation and restored the antioxidants to normal. The present study reveals that Eclipta alba showed effective anti-arthritic activity through its antioxidant and anti-inflammatory property. Further the anti-arthritic activity of E. alba might be due to the presence of various phytochemicals such as flavanoids and polyphenols.

Total and differential leukocyte count and oxygen saturation of hemoglobin changes in healthy smokers and non-smokers

Background: Inflammatory markers are altered in smokers and smoking has been proved as one of the main causative factors for cardiovascular diseases (CVDs), inflammatory diseases, and oxidative stress stimulation. An alteration in hematological parameters and oxygen saturation of hemoglobin is expected with smoking tobacco. Aim and Objective: This study has been planned with the aim to evaluate the changes associated with the extent of adverse effects of tobacco smoking in total and differential leukocyte count (DLC) and oxygen saturation of hemoglobin in healthy smokers and non-smokers. Materials and Methods: A total of 150 healthy adults were participated in this cross-sectional study. Anthropometric measurements such as height, weight, and body mass index were taken, and information of smoking habits were obtained by a questionnaire. Blood samples were taken for the estimation of total and DLC using MS-9 automated hematology cell counter. Oxygen saturation of hemoglobin was done using fingertip pulse oximeter Nidek Medical 5300. Results: Our results showed a statistically significant rise in the total leukocyte count (TLC) (P < 0.001), lymphocyte count (P < 0.001), granulocyte count (P < 0.01), and monocyte count (P < 0.02) and showed a statistically significant decrease in SpO2 (P < 0.04) in smokers as compared to non-smokers. Conclusion: The study has shown that altered values of TLC and DLC and oxygen saturation of hemoglobin in smokers should be considered during diagnosis, interpretation of result, and treatment of patients. A high TLC and DLCs exhibited in this research may be responsible for chronic inflammation and subsequent high risk of CVD in smokers. Therefore, quitting smoking should be encouraged for better health.

Combined administration of exogenous melatonin and insulin ameliorates streptozotocin induced toxic alteration on hematological parameters in diabetic male Wistar rats

The aim of the present was to ameliorate the protective effect of exogenous melatonin and insulin against the diabetes induced alterations in the different hematological variables. Albino rats were administrated streptozotocin at the dose of 15 mg/kg for 6 days. Total 54 rats were randomly selected for the experimental purpose and were divided into two major groups. Group-1 consisting twenty four (24) and were further sub-divided into four (4) different groups viz. group-I served as normal control, group-II served as melatonin treated, group-III served as insulin treated and group-IV served as glibenclamide treated. Group-2 consisting thirty (30) rats were given streptozotocin (STZ) injection (15 mg/kg) for 6 days. After confirmation of diabetes by measuring blood glucose level, animals having blood glucose level above 250 mg/dl) confirmed as diabetic. Thirty (30) Diabetic rats were further subdivided into following sub-groups and were given different therapeutic treatments, Viz group-I served as Diabetic control, group-II treated with melatonin, group-III treated with insulin, group-IV given treatment of melatonin and insulin and group-V were given treatment of glibenclamide respectively. Diabetic rats showed modulation in all the studied hematological variables. Diabetic rats displayed significant decline in RBCs count, HB level and its associated indices (HCT, RDW, MCV, MCH, MCHC), WBCs and its related indices (polymorphs and lymphocytes) and platelet distribution width (PDW %) whereas platelet count showed significant increase. Nonetheless alone as well as combined treatment of exogenous melatonin and insulin restored all altered hematological parameters. However, significant recovery was found in the group in which combined dose of melatonin and insulin was administrated. Therefore, it might be concluded that combined administration of melatonin and insulin will be better remedy to normalize the altered blood profile during the diabetic condition.

Potential Curative Effect of Curcumin on Gastric Ulcer Induced by Piroxicam in Male Albino Rats

This study intended to investigate the curative effect of curcumin on piroxicam-induced gastric ulcer in rats. Twenty-seven male albino rats were allocated into three similar groups (9 of each). The firstgroup (G1) was the control, G2 received piroxicam (30 mg∕ kg BW.) to induce gastric ulcer and G3 received piroxicam (30 mg∕ kg BW.) and on the third day it received curcumin (200 mg ∕ kg BW.) orally for 21 day. At the end of the experiment, the rats were humanly euthazied and blood and serum samples were taken for haematological and biochemical analysis, respectively. Subsequently, the stomachs were opened along their curvature for evaluation of ulceration, gastric juice was measured and gastric samples were preserved in 10% neutral buffered formalin for histopathological examination. Piroxicam administration induced gastric ulceration (100%), non-significant changes in the gastric juice volume and a remarkable decrease in the number of red blood cells, hemoglobin concentration and packed cell volume with a significant increase in platelets count, white blood cells, neutrophils, eosinophils and monocytes. Furthermore, it displayed a significant reduction of glutathione peroxidase, superoxide dismutase and catalase activities with a significant increase in malondialdehyde and tumor necrosis factor alpha concentrations. Histopathological results revealed focal mucosal erosive and ulcerative changes with sever hyperemia of stomach in piroxicam treated group (G2). Oral administration of curcumin treated the piroxicam induced gastric ulceration and improved the altered hematological parameters, oxidant ∕ antioxidant status and histological pictures of rats in G3. Therefore, curcumin may offer an attractive strategy for treatment of gastric ulcer.

Trema orientalis (Linn.) leaves promotes anticancer activity in Ehrlich ascites carcinoma (EAC) in Swiss albino mice.

Background The in vivo anticancer effect of the Trema orientalis leaves crude methanol extract (TLME) was screened against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Materials and methods The cytotoxic activity of TLME was determined in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The growth inhibitory activity and morphological alterations were determined by the hemocytometer counting of the EAC cells using trypan blue dye. The apoptotic cells were assessed by DAPI (4',6-diamidino-2-phenylindole) staining. The hematological and biochemical parameters of experimental mice were also estimated. Results After treatment with the TLME, the viable tumor cell count, morphological changes and nuclear damages of the EAC cells were observed along with the hematological parameters of the experimental mice. The LD50 of TLME was 3120.650 mg/kg body weight, and this extract was proven to be safe at a dose of as high as 800 mg/kg body weight. The oral administration of the TLME at 400 mg/kg body weight resulted in approximately 59% tumor cell growth inhibition compared with the control mice, with considerable apoptotic features, including membrane blebbing, chromatin condensation, nuclear fragmentation and aggregation of the apoptotic bodies in DAPI staining under a fluorescence microscope. The TLME also dose-dependently restored the altered hematological parameters to approximately normal levels. The TLME exhibited bolstering cytotoxic effect against the EAC cell with the IC50 value of 29.952 ± 1.816 μg/mL. Conclusion The TLME has potential as a natural anti-cancer product with apoptosis induction property and cytotoxicity against carcinoma cells.

Hematologic Parameters and Blood Cultures from the Gingival Vein Compared with the Cranial Vena Cava in Guinea Pigs.

Blood collection methods in guinea pigs are limited due to the animals' compact neck, short limbs, and lack of a tail. Gingival venipuncture is a recently described blood sampling technique that is minimally traumatic with no significant alterations in hematologic parameters when multiple blood samples were collected weekly for 6 wk. The purpose of this study was to determine whether the gingival vein can be used as an alternative blood collection site in guinea pigs, such that: (1) hematologic parameters would be consistent with samples collected from the cranial vena cava; and (2) no contaminants from the oral cavity would be introduced into the sample. Blood samples were obtained from both the gingival vein and cranial vena cava of anesthetized Dunkin Hartley guinea pigs for CBC (n = 9) and aerobic blood cultures (n = 10). Only MCV was significantly different between sampling sites. Bland-Altman analyses calculated a small mean bias for all hematologic parameters, indicating clinical interpretation is unlikely to be affected by the sampling site. Bacterial growth occurred in all 5 gingival vein blood samples prepared by using saline and 2 of the 5 prepared with dilute chlorhexidine. Bacteria did not grow from any cranial vena caval blood samples prepared with dilute chlorhexidine. No clinical signs of hemorrhage or trauma were detected at either site. These results provide evidence that gingival venipuncture can be used as an alternative blood collection method for guinea pigs for hematologic analysis but should not be used for blood culture.

Assessment of Toxicity and Therapeutic Effects of Goose Bone in a Rat Model

Goose bone is traditionally used in the treatment of many ailments including in bone fracture. The aim of the present study was to evaluate the subacute toxicity of goose bone in a rat model by investigating some hematological and biochemical parameters in rats. Subsequently, a histopathological study was performed to confirm the presence of pathological lesions in the rat’s vital organs including the liver, kidney, heart, brain, pancreas, lung, spleen, and stomach. Adult Wistar rats were divided into four groups (n = 8) and were orally administrated with three doses (30, 60, and 120 mg/kg) of goose bone once daily for 21 days as compared to control animals (received only drinking water). Goose bone did not cause any significant changes on body weight, relative organ weight, and percentage water content at any of the administered doses. There were also no significant alterations in hematological parameters seen. All three doses administered significantly reduced the triglyceride levels as well as the atherogenic index of plasma (AIP). Animals treated with 120 mg/kg doses had significantly reduced alkaline phosphatase (ALP) activity as compared to the control group. There was no significant alteration on other serum biochemical parameters seen. Additionally, histopathological findings confirmed that there was no inflammatory, necrotic, or other toxicological feature seen for all three doses. It is concluded that goose bone is nontoxic and is safe for consumption besides having the potential to be investigated for the treatment of high triglycerides or liver-related disorder.

Selenium nanoparticles act as an intestinal p53 inhibitor mitigating chemotherapy-induced diarrhea in mice

Selenium, at high-dose levels approaching its toxicity, protects tissues from dose-limiting toxicities of many cancer chemotherapeutics without compromising their therapeutic effects on tumors, there by allowing the delivery of higher chemotherapeutic doses to achieve increased cure rate. In this regard, selenium nanoparticles (SeNPs), which show the lowest toxicity among extensively investigated selenium compounds including methylselenocysteine and selenomethionine, are more promising for application. The key issue remains to be resolved is whether low-toxicity SeNPs possess a selective protective mechanism. p53 or p53-regulated thrombospondin-1 has each been confirmed to be an appropriate target for therapeutic suppression to reduce side effects of anticancer therapy. The present study demonstrated that SeNPs transiently suppressed the expression of many intestinal p53-associated genes in healthy mice. SeNPs did not interfere with tumor-suppressive effect of nedaplatin, a cisplatin analogue; however, effectively reduced nedaplatin-evoked diarrhea. Nedaplatin-induced diarrhea was associated with activation of intestinal p53 and high expression of intestinal thrombospondin-1. The preventive effect of SeNPs on nedaplatin-induced diarrhea was correlated with a powerful concomitant suppression of p53 and thrombospondin-1. Moreover, the high-dose SeNPs used in the present study did not suppress growth nor caused liver and kidney injuries as well as alterations of hematological parameters in healthy mice. Overall, the present study reveals that chemotherapeutic selectivity conferred by SeNPs involves a dual suppression of two well-documented targets, the p53 and thrombospondin-1, providing mechanistic and pharmacologic insights on low-toxicity SeNPs as a potential chemoprotectant for mitigating chemotherapy-induced diarrhea.

Hematological and biochemical parameters in Sea turtles (Caretta caretta) after stranding

The turtle Caretta caretta is the most abundant of sea turtles that reproduces in the Mediterranean Sea. Caretta caretta is also classified as a vulnerable species and the Mediterranean population in the past years has been in decline. The danger for these marine organisms is linked to the anthropization of the coasts, to marine traffic, to the pollution of the sea, above all due to the presence of plastic debris and threats related to fishing.The aim of this study was to document the state of health of these animals after stranding. We examined whether hematological and biochemical parameters determine the survival of the individual or not. We highlighted how anthropic pressure continues to alter the typical ecosystems of the Mediterranean Sea.Turtles that cannot survive after stranding show altered hematological and biochemical parameters compared to surviving individuals. This highlights the presence in these animals of severe anemia, kidney damage (evidenced by increased level of uric acid and alanine transferase) and cellular necrosis in various tissues (high levels of aspartate aminotransferase and Lactate dehydrogenase).The results obtained show that the hematological and biochemical parameters can be a good tool to evaluate the possibility of survival of stranded turtles.

Barium chloride dose-dependently induced heart and lung injury in Wistar rats.

Barium (Ba) is one of the environmental pollutant metals that incite deleterious effects on human health. The present study investigated the effects of exposure to different doses of barium chloride (BaCl2 ) on heart and lung of Wistar rats. Rats were exposed to BaCl2 at 150, 300, and 600 mg/L for seven consecutive days. Results indicated that exposure to Ba caused heart and lung damage evidenced by significant increase in plasma lactate dehydrogenase and creatine kinase activities, total cholesterol, triglyceride, and low-density lipoprotein-cholesterol levels, while high-density lipoprotein-cholesterol level decreased when compared with control. Moreover, BaCl2 significantly decreased superoxide dismutase, catalase, and acetylcholinesterase activities as well as glutathione level in heart and lung of the treated rats. Furthermore, the dose-dependent increase in cardiac and lung lipid peroxidation, advanced oxidative protein product and nitric oxide levels were accompanied by marked increase in metallothionein in the BaCl2 -treated rats. Administration of BaCl2 altered hematological parameters and significantly increased concentrations of interleukin-6 in the treated rats. Histology analysis showed significant alteration in the heart and lung tissues of Ba-treated rats. In conclusion, BaCl2 -induced heart and lung damages via disruption of antioxidant defense systems, and activation of inflammatory mediators and alteration in hematological parameters in rats.

A comparative study assessing the effect of haematological and biochemical parameters on the pathogenesis of malaria

Malaria is one of the most common parasitic disease affecting mankind since millennia. The most pronounced changes related to malaria involve the blood and the blood forming system, the spleen and the liver. The abnormal haematological and biochemical parameters observed in malaria cases adversely affect the prognosis of the disease. The aim of this study was to assess the severity of malaria by observing the significant abnormalities in haematological and biochemical parameters in the malaria infected cases as compared to the healthy controls. The study population comprised of 138 individuals, of which 69 were malaria cases and 69 were apparently healthy controls. All the 138 individuals were subjected to haematological and biochemical workup, following which statistical analysis was done to observe any association of altered haematological and biochemical parameters with severity of malaria, as compared to the healthy controls. Among the 138 study population, 69 patients were malaria cases whereas the other 69 were healthy controls. Haematological investigations revealed, that the haemoglobin levels, total RBC counts and haematocrit were significantly altered in the malaria cases as compared to the healthy controls. Also the leucogram profile showed significant leucopenia and neutropenia in the malaria patients as compared to the controls. Thrombocytopenia was also seen to be more pronounced in the malaria infected. The liver enzymes and serum bilirubin levels were raised in the malaria cases more than the controls. Altered haematological and biochemical parameters are indicators of disease progression to severity. Early detection and management of these parameters, will prevent the development of complications in malaria.

Increased DNA damage is not associated to polymorphisms in OGGI DNA repair gene, CYP2E1 detoxification gene, and biochemical and hematological findings in soybeans farmers from Central Brazil

Our study evaluated 163 individuals, being 74 soybean farmers, occupationally exposed to pesticides, and 89 individuals from Goias municipalities, Central Brazil, with similar conditions to the exposed group, comprising the control group. Of the 74 soybean farmers, 43 exposed directly to pesticides and 31 exposed indirectly. The exposed group consisted of individuals aged 19 to 63 years, 21 women and 53 men, and the control group had ages ranging from 18 to 64 years, being 36 women and 53 men. 18.9% of the exposed group were poisoned by pesticides, and the most common symptoms were headache and gastrointestinal problems. The genotype frequencies of the rs2031920 (T>C) polymorphism in the CYP2E1 gene present significant differences between the exposed and control groups (p = 0.02), showing that 24.3% of the exposed group were heterozygotes against 6.7% in the control group. For the OGG1 gene, two SNPs, rs1052133 (G>C) and rs293795 (T>C), were evaluated and the genotype frequencies were not statistically different between the exposed and control groups. The DNA damage was distinct (p < 0.05) in the three analyzed comet parameters (tail length, Olive tail moment, %DNA) between groups. However, there was no influence of age and alcohol consumption between the groups associated with the polymorphisms in the CYP2E1 and OGG1 genes and DNA damage. We also did not find altered hematological and biochemical parameters in the exposed group. Thus, this pioneering study at Goias State carried out an overview of the health of soybean farmers. We evaluated classic laboratory exams, associated with exposure markers (comet assay) and susceptibility markers (genetic polymorphisms), emphasizing the need to expand the Brazilian health assessment protocol. We found, in soybean farmers, increased DNA damage and a higher number of heterozygotes in CYP2E1 gene, compared with the control group, despite the lack of association with age, educational level, smoking, drinking habits, and genetic polymorphisms.