Alpinia Galanga Research Articles

Simple preparation of the dried galangal (Alpinia galanga) in ethanol is effective to suppress Curvularia sp., which causes grain discoloration of rice (Oryza sativa ‘Suphan Buri 2’)

ISHS IX International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation (physiological, biochemical, embryological, genetic and legal aspects) Simple preparation of the dried galangal (Alpinia galanga) in ethanol is effective to suppress Curvularia sp., which causes grain discoloration of rice (Oryza sativa ‘Suphan Buri 2’)

In vitro screening of crude extracts from plants against Fusarium sacchari and Curvularia lunata and testing the efficacy of a selected crude extract of Alpinia galanga against grain discoloration and other rice diseases in the fields

In vitro screening of crude extracts from plants against <i>Fusarium sacchari</i> and <i>Curvularia lunata</i> and testing the efficacy of a selected crude extract of <i>Alpinia galanga</i> against grain discoloration and other rice diseases in the fields

Green synthesis of silver nanoparticles from Alpinia galanga extract with microwave irradiation and antibacterial activity against Escherichia coli

Background: This study deals with the rapid green synthesis of silver nanoparticles (Ag NPs) by microwave irradiation using water extract of Alpinia galanga as a reducing agent. Methods: The effect of concentrations of water extract and silver nitrate aqueous solution (1 mM) on the synthesis was investigated using the ratios 1:1, 1:5, 1:10, and 1:20. The formation of Ag NPs was observed after being microwaved for 30, 60, 90, 120, 150, and 180 seconds. Results: The best Ag NPs were produced from the ratio of 1:10 after 180 s irradiation, confirmed by the optimum absorbance at 450 nm using a UV-visible spectrophotometer. Transmission electron microscopy analysis showed that the size of the Ag NPs was 50 nm. Their antibacterial activity was determined using Resazurin microtiter assay against Escherichia coli, and the results showed that it inhibited 94.4% of E. coli. It was significantly different (p &lt; 0.05) with the inhibition of extract and Amoxsan, which inhibited 71.1% and 85.8% of E. coli, respectively. Conclusion: The use of microwave irradiation on the synthesis of silver nanoparticles using Alpinia galanga extract as a reducing agent appears to be rapid and eco-friendly.

In silico ADMET analysis, Molecular docking and in vivo anti diabetic activity of polyherbal tea bag formulation in Streptozotocin-nicotinamide induced diabetic rats

Ichnocarpus frutescens, Ficus dalhousie, Crateva magna, Alpinia galangal, and Swertia chiraita are well-known plants available throughout India and they are commonly used for the treatment of various diseases including diabetes mellitus. The antidiabetic activity of the individual plant parts is well known, but the synergistic or combined effects are unclear. Polyherbal formulations enhance the therapeutic action and reduce the concentrations of single herbs, thereby reducing adverse events. The aim of the present study is to formulate a polyherbal tea bag formulation and evaluate its in vivo antidiabetic potential. The polyherbal formulation was formulated using the crude powder of the plants. The herbal formulation depicts hyperglycemic effects in both normal and experimentally induced hyperglycemia rats. The antidiabetic activity of the polyherbal formulation (100 mL/kg b.w.) was screened against streptozotocin-nicotinamide induced diabetes mellitus in rats. The infusion extract was administered for 21 consecutive days, and the effect of the polyherbal formulation on blood glucose levels was studied at regular intervals. At the end of the study, the blood samples were collected from all the animals for biochemical estimation (SGOT and SGPT). Polyherbal tea bag formulation showed significant antidiabetic activity and this effect was comparable with that of Metformin.

Physicochemical, Phytochemical and HPTLC Analysis of a Novel Combined Herbal Formulation

Background: Cinnamomum zeylanicum (Darchini), Alpinia galanga (Khulanjan), and Withania somnifera (Asgand) have been used in joint diseases by Unani scholars since long. The combination of these ingredients may be more effective than they are given alone as these drugs are having potent anti-inflammatory, antioxidant, analgesic and rejuvenating properties. As per the WHO guideline, standardization should be done before undertaking any experiment which claims safety and efficacy. Therefore, this study is aimed to establish the physicochemical and phytochemical quality standards for this novel combination of herbal formulation for future references. Methods: In present study, extractive values such as methanol extract, aqueous extract, chloroform extract and aqueous ethanol extract was obtained. Further, loss on drying, pH, ash values, fluorescence analysis, qualitative phytochemical analysis and HPTLC fingerprint profile were done. Results: Mean values of methanolic, water, chloroform, and aqueous ethanolic extracts were 10.4±0.77%, 13.0±1.3%, 2.3±0.26% and 15.28±1.18% respectively. Further, the total ash, acid insoluble ash, and water-soluble ash were 3.3±0.32%, 1.13±0.09%, and 1.72±0.2% respectively. pH of 1% extract was 5.57±0.16 and 5.37±0.32 for 10% extract. It was observed that the percentage of moisture content was 4.67±0.42%. Qualitative phytochemical analysis revealed that the extract contains carbohydrates, glycosides, phenolic compounds, flavonoids, proteins and amino-acids, alkaloids, and saponins. Further, HPTLC fingerprint revealed the presence of many plant metabolites and active ingredients. Conclusion: The data generated in this study have been reported for the first time for this novel drug combination, which could be used as a source for future studies.

UPAYA PENINGKATAN PRODUKTIVITAS GETAH PINUS (Pinus merkusii) MENGGUNAKAN STIMULANSIA EKSTRAK LENGKUAS (Alpinia galanga) DAN JAHE (Zingiber officinale)

With the increasing world demand for pine resin, one of the efforts that can be made to increase the production of pine resin is to use stimulants of sap / stimulants. So far, in the activity of tapping pine sap, inorganic stimulants are used, but the main components are sulfuric acid and nitric acid or a mixture of which is a strong oxidizing agent that can damage human skin, wood and the environment if used in the long term and excessively. Alternative use of stimulants from natural ingredients that are safe, relatively inexpensive, and easy to obtain, such as galangal (Alpinia galanga) and ginger (Zingiber officinale). This research was conducted to determine the effect of stimulant administration of galangal and ginger extracts on increasing the productivity of pine sap. The results of this study showed that the highest sap productivity was produced by pine trees using ginger organic stimulants with a 50% concentration of 11.15 g / koakan / day. The quality of pine sap that has the best quality is produced by the stimulant of ginger, while the use of the stimulant of galangal has second quality based on SNI 7837: 2016.

Efficacy of Different Solvents on the Extraction Process of Antioxidant and Potential Compounds in Alpinia Galanga L. from Indonesia

Objectives: To determine the most effective solvent for extraction of antioxidant and potential compounds in Alpinia galanga, expressed via the percentage of components in the sample. Methods: A comparison test of 4 extractants, including water, hexane, chloroform and ethyl acetate, was conducted for the extraction procedure. In addition, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to perform antioxidant experiments. HPLC and GC-MS were then employed to determine quercetin content and scan the chemical compositions of the extract, respectively. Results: Ethyl acetate extract showed the strongest antioxidant activity (DPPH IC50=127.67ppm, ABTS IC50=54.82ppm), containing the highest level of quercetin (39.61mg/g DW of extract) and 2 major components of methyl oleate (15.36%) and methyl palmitate (12.01%). In the correlation analysis, the antioxidant activity of Alpinia galanga extracts was proportional to its total phenolic, flavonoid, and quercetin contents. Conclusion: Ethyl acetate produces the best performance in extracting anti-oxidative agents and quercetin in Alpinia galanga. The presence of quercetin and FAMEs in the extract highlighted the potentials of antioxidant, antihypertensive, anti-inflammatory, antibacterial, antiviral, and antidiabetic properties of Alpinia galanga.

Subchronic toxicological evaluation of EnXtra™ (standardised extract of Alpinia galanga rhizome) in rats.

Alpinia galanga is a commonly used ingredient in Asian food and traditional medicine. But an extract of the rhizome had never been used commercially in food supplements and functional foods. There is some evidence of safety and tolerability in humans for a proprietary A.galanga rhizome extract (EnXtra™) and it is Generally Recognised as Safe (GRAS) in the US already. However, this botanical ingredient has not been evaluated for its subchronic toxicity in rats to confirm its safety in wider food applications. Sprague Dawley rats were orally administered the test item for 90days by following OECD (Test Guideline: 408), with a recovery period of 28days. Cumulative effects and No Observed Adverse Effect Level (NOAEL) were estimated. EnXtra™ was administered orally at 0, 1,000, 2,000 and 3,000mgkg-1 body weight (b. wt.) with additional vehicle and high dose recovery groups. Observations included clinical signs, haematology, clinical chemistry, gross pathology and histopathology. On terminal sacrifice, no treatment-related adverse effects were observed viz., clinical signs, mortality, body weight changes and feed consumption parameters. Haematology, clinical biochemistry and thyroid hormone levels were within the normal range. Further, no treatment-related gross and microscopic pathological lesions were observed across the treatment groups. Based on the results of the toxicological evaluation, NOAEL of A.galanga rhizome extract (AGRE) was fixed at 3,000mgkg-1b. wt. per day and ADI of 1800mg day-1 in the case of humans.

Alpinia galanga Extract Inhibits MCF-7/HER2+ Cells by Inducing Apoptosis

Background: Cancer is a disease caused by abnormal growth of body tissue cells. Cancer treatment strategy by induce apoptosis and inhibit proliferation.&#x0D; Aim: This research aims to examine the anticancer effect of Alpinia galanga extract on MCF-7/HER2+ breast cancer cells. &#x0D; Method: The MTT Assay cytotoxic test was carried out to determine the growth inhibitory activity. Apoptotic assay to determine the activity of compounds in apoptosis, as well as through a structural approach utilizing various virtual platforms to monitor its activity.&#x0D; Result: Based on the MTT assay Alpinia galanga extract possessed cytotoxic effect in dose-dependent manner with IC50 value of 330.79 μg/mL. The extract induces apoptosis of MCF-7 cells up to 25.56%.&#x0D; Conclusion: It can be concluded that Alpinia galanga extract demonstrated cytotoxic activity toward MCF-7/HER2+ breast through apoptosis induction. This extract had an opportunity to be developed as a potential anticancer agent to overcome breast cancer diseases.

Evaluation of the anti-dyslipidemic effect of spice fixed oils in the in vitro assays and the high fat diet-induced dyslipidemic mice

Dyslipidemia is a condition in which the lipid levels are altered in the body. The fixed oils (FOs) from spices, Alpinia galanga, Cinnamomum zeylanicum, Trigonella foenum-graecum, Foeniculum vulgare, and Myristica fragrans were evaluated as potential sources of anti-dyslipidemic activity. The nutraceutical compound fractions such as polyphenols, tocopherols, γ-oryzanol, and phytosterols were extracted from spice FOs, tested individually and in combination for cytotoxicity and their effect on lipid accumulation in 3T3-L1 and Hep-G2 cell lines. Further, the best performing FOs in the in vitro studies were fed to high fat diet-induced dyslipidemic C57BL/6 mice and studied its activity in the liver and adipose tissue. The in vitro assays showed no cytotoxicity in the studied FOs up to 200 μg/mL concentration. Out of all the studied nutraceutical compound fractions, the γ-oryzanol and phytosterols fractions showed a reduction in the accumulation of lipid droplets in 3T3-L1 cell lines. Moreover, the reconstituted nutraceutical fractions from C. zeylanicum, T. foenum-graecum, and F. vulgare FOs exhibited a dose dependent inhibition of lipid accumulation compared to the fractions of A. galanga and M. fragrans FOs. The in vivo studies confirmed the anti-dyslipidemic activity of C. zeylanicum FO that showed an improved lipid profile, antioxidant enzymes, and reduced lipid droplets in the liver and adipose tissue. The histopathological images of the liver and adipose tissue showed a comparable visualisation in Atorvastatin, and C. zeylanicum fed group mice. Hence, the spice FOs are potentially efficacious anti-dyslipidemic functional food ingredients and could be safer than statins.

ANALISIS USAHATANI LENGKUAS (Alpinia galanga) DI KELURAHAN SUKA MULYA KECAMATAN SEMATANG BORANG KOTA PALEMBANG PROPINSI SUMATERA SELATAN

Analysis of Lengkuas Farming (Alpinia galanga (L) Sw) case study in Sukamulya Subdistrict Sematang District Borang Palembang City.  This study aims to find out how much income and profits galangal farming is implemented in Sukamulya Village District Sematang Borang Palembang City, This study was conducted from February to June 2018.Population in this research is farmer of lengkuas in Sukamulya Subdistrict of Sematang District Borang Palembang as many as 60 farmers. Sampling is using simple random sampling (Simple Random Sampling) of 30% of the total population of 18 farmers as a respondent lengkuas. The calculated operational definitions are fixed costs including land lease fees and equipment depreciation. Variable costs include purchasing seeds, fertilizers, labor in the family. Production of galangal, receipts received by farmers per year, farmer's income and profit galangal farming in Sukamulya Village Sematang District Borang Palembang City. The results showed that the average production cost of sample farmers in Sukamulya village was Rp 49.393.594, -/Ha/Planting season. Receipt of farmers in Sukamulya Village Sukamulya District Borang Palembang City an average of Rp 77.385.000, -/Ha/Planting season. The average income of lengkuas farming in Sukamulya sub-district of Sematang Borang sub-district of Palembang is Rp. 27.991.406, -/Ha/Planting season. The result of analysis of farmer farming system in Sukamulya Village Subdistrict Sematang Borang City Palembang R/C is obtained at the farmer of example of galangal farming of 1.57 means that every Rp 1000, - the cost incurred by farmer, will get the acceptance of Rp 1,570,-. So the cultivated galvanized farming is profitable because R/C &gt;1. Key words : Farming, Lengkuas, Cost and Marketing Flow

Utilization of Rhizome (&amp;lt;i&amp;gt;Alpinia galangal&amp;lt;/i&amp;gt; L.) in Improvement of Some Quality Attributes of Some Processed Meat

Rhizome Galangal (Alpinia galanga L.) is considered a natural source of fiber, minerals and natural antioxidant compounds. This research was performed to study. The effect of adding galangal powder in different proportions (1.5, 2.5, and 3.5 gm.). Also, the effect of adding galangal extract (5%, 10% and 15%) in proportions from the total formula of beef burger patties on chemical, physiochemical, microbiological and sensory characteristics of beef burger patties during the frozen storage period at -18°C ± 2°C up to 6 months. The obtained results showed that galangal contained crude fibers (14.00%), Moisture (5.43%), K (1108.13 ppm), Mg (970 ppm), P (729.21 ppm) and Ca (370.81 ppm), while Fe (58.53 ppm) and Na (33.74 ppm) as macro and micro elements. The percent of antioxidants was about (92.60%), phenols (123.81 mg/g) and flavonoids were about (109.77 mg/g) was higher in galangal extract than galangal powder, which was recorded in its antioxidants (72.95%), phenolic (47.66 mg/g) and flavonoids (34.02 mg/g), as well as the improvement of physiochemical quality criteria (pH, protein and TBA values) throughout frozen storage in comparison with the control sample. In addition, galangal inhibited the growth and activity of microbial oxidation in tested prepared product. Also, beef burger patties containing the galangal exhibited a good sensory property and palatable, especially those containing (2.5 and 3.5 g) galangal powder and (10% and 15%) galangal extract, even after storage for 6 months under frozen conditions. The present results are useful for using galangal in the fortification of some meat products to improve nutritionally and healthy safe.

Recent Findings on Alpinia Galanga (L.) Wild for the Treatment of Arthritis Part-1

Arthritic inflammation of joints affects people of all age groups. The treatment is a challenge as arthritis is a complex disease and evolves over years. Patients often have to take medicines for the rest of their life. Commonly prescribed medicines like analgesics, glucocorticoids and non-steroidal anti-inflammatory drugs have side effects. The new disease-modifying medicines are costly. People in the healthcare system are assessing the dynamics of complementary and alternative medicines. One such remedy is Alpinia galanga (AG) of Zingiberaceae family. It is characterized by the presence of rhizome, wide leaves and terminal inflorescence. The references for its medicinal uses are found in traditional medicines. It is cultivated in tropical regions of south east Asia. Its rhizome is highly aromatic and most frequently used as a food and medicine. The various extracts of AG are prepared by the researchers and studied for its phytoconstituents and pharmacological activities. Clinical, in vitro, in vivo and in silico experimentation techniques are used to validate the claims for various therapeutic activities. This article focuses on reviewing the literature to ascertain the anti-arthritic potential of AG. The article has been divided in 2 parts and includes analgesic, anti-arthritic, anti-inflammatory, antioxidant, other therapeutic effects as well as safety and toxicity of AG.

Recent Findings on Alpinia Galanga (L.) Wild for the Treatment of Arthritis Part-2

Arthritic inflammation of joints affects people of all age groups. The treatment is a challenge as arthritis is a complex disease and evolves over years. Patients often have to take medicines for the rest of their life. Commonly prescribed medicines like analgesic, glucocorticoids and non-steroidal anti-inflammatory drugs have side effects. The new disease-modifying medicines are costly. People in the healthcare system are assessing the dynamics of complementary and alternative medicines. One such remedy is Alpinia galanga (AG) of Zingiberaceae family. It is characterized by the presence of rhizome, wide leaves and terminal inflorescence. The references for its medicinal uses are found in traditional medicines. It is cultivated in tropical regions of south east Asia. Its rhizome is highly aromatic and most frequently used as a food and medicine. The various extracts of AG are prepared by the researchers and studied for its phytoconstituents and pharmacological activities. Clinical, in vitro, in vivo and in silico experimentation techniques are used to validate the claims for various therapeutic activities. This article focuses on reviewing literature to ascertain the anti-arthritic potential of AG. The article has been divided in 2 parts and includes analgesic, anti-arthritic, anti-inflammatory, antioxidant, other therapeutic effects as well as safety and toxicity of AG.

In vitro Inhibitory Effect on Alpha Amylase Enzyme by Polyherbal Dip Tea in Diabetes

The presentstudy aims to preparenovel polyherbal formulations in aunique and accessible tea bag form and also analysesthe antidiabetic potentials in vitro.Material and method:The formulation wasmade from Ichnocarpus frutescens(IC), Ficusdalhousiae (FD),Cretevamagna (CM),Alpiniagalanga (AG)andSwertiachirata (SC)herbs andused in this study. Indian medicinal plants i.e.,polyherbalwere subjected to progressivephytochemical analysis and testswere carried outfor the determination of α-amylase inhibitionactivity.The Phytochemical analysis revealedthepresence of Alkaloids,Tannins,Anthraquinone, Flavonoids,Saponinand reducing sugar, as the active compounds. The tea infusions of the aforementioned plants were prepared in differentformulations like F1, F2, F3, and F4. The formulations weretested for antidiabetic activity in vitro through the inhibition of alpha-glycosidase.Results: TheF3 (91.6%) and F4 (92.7%) showedthehigher inhibition data againstporcine pancreatic α-amylase among the Fourformulations. The minimum% alpha-amylase inhibition was recordedin F2 (90.4%)amongthe medicinalactiveplants studied.Conclusion: Hyperglycaemia level is reducedvia a controllable condition of starch breakdown through pancreaticα-amylase inhibitors.Best results were obtained by the infusion containing The F2 formulation which showed significant antidiabetic activity.©2022iGlobal Research and PublishingFoundation. All rights reserved.

The Effect of Alpinia Galanga Extract to Seminiferous Tubular Epithelial Thickness and Seminiferous Tubular Diameter of Male Mice Induced to Lead Acetate

The purpose of this research was to determine the effect of Alpinia galanga extract for seminiferous tubular epithelial thickness, and seminiferous tubular diameter of mice induced to lead acetate. This study was experimental, 25 male mice (Mus musculus) strain Balb/C with average arround 30 gram were divided into 5 groups in a completely randomized design with different treatments for 24 days. The negative control group C (-) was given CMC-NA, the positive control group C(+) was given CMC-NA + lead acetate, groups P1, P2, and P3 were given galangal extract 200, 400, and 800 mg/kgBB + CMC-NA + lead acetate. Mice were sacrificed at a predetermined time and then histopathological preparations of testicular organs were made for epithelial thickness and seminiferous tubule diameter. Data analysis used ANOVA test and continued with LSD test. The result of the research galangal extract administration showed an increase in epithelial thickness and diameter significantly by the three treatment groups against the control (+) (p&lt;0.05). group P2 which given galangal extract 200 mg/kgBw/day is the most effective to prevent epithelial thickness (59.83 ± 0.48; p&lt;0.05) and diameter of seminiferous tubules (180.89 ± 0.85; p&lt;0.05) because the result not significantly different from the control group (-) where epithelial thickness (59.64 ± 1.83; p&lt;0.05) and seminiferous tubule diameter (180.71 ± 1.37; p&lt;0.05) were given CMC- NA 0.2 ml/day. It can be concluded that giving galangal extract to male mice exposed to lead acetate was proven to maintain thick epithelium and seminiferous tubule diameter.

The Effect of Alpinia Galanga Warm Compress in Reducing Rheumatoid Arthritis Pain Intensity in the Elderly: A Quasi-Experimental Study

Backgrounds: Rheumatoid arthritis is a chronic inflammatory disease that causes inflammation and swelling of the synovial membrane. In patients with advanced stages, it will make the patient unable to carry out daily activities and his quality of life decreases.The study at to know the effect of Alpinia Galanga Warm Compress in Reducing Rheumatoid Arthritis Pain Intensity in the Elderly.Methods: The method of the research was used quasi experiment by using one group pre-test and post-test design without control group. This study was conducted on 45 patients with Rheumatoid Arthritis in Guna Bakti Foundation in Medan, Indonesia. The research process began with measuring the intensity of pain in all of patient using an observation sheet and numerical pain intensity scale before being given a warm galangal compress (pretest), then a warm galangal compress was carried out for 20 minutes, after which it was measured again (posttest) the intensity of the pain scale. Results: Based on the results of the study, 80% of respondents experienced a decrease in the Rheumatoid arthritis pain scale as a result of giving warm galangal compresses. From the significance value of the Wilcoxon test results obtained p-value &lt;0.001 which value is smaller than the error level (α) 0.05 or with a significance of 95%, then the hypothesis Ho is rejected while Ha is accepted. The conclusion of this study is the effect of Alpinia galanga warm compress in reducing rheumatoid arthritis pain (p&lt;0.001).Conclusion: Galangal warm compresses are effective in reducing Rheumatoid Arthritis pain. Therefore, such interventions are recommended for these patients.

Individual or Combination Impacts of Alpinia galangal and Zinc Sulfate on Growth Performance, Digestibility, and Carcass Traits of Weaning Male Rabbits

Individual or Combination Impacts of Alpinia galangal and Zinc Sulfate on Growth Performance, Digestibility, and Carcass Traits of Weaning Male Rabbits

Preservation Potential of Galangal Water Extract on Tofu

Galangal (Alpinia galanga (L.) Willd.) has been long used for medicinal purposes. It showed promising effects on the food-spoilage microorganisms and is commonly used as spices in various cuisines. This study aimed to evaluate the microbial growth inhibitory activity and the preservation potential of galangal water extracts on tofu. The water extracts were prepared by infundation method in the concentrations of 5, 10, and 20%w/v. The phytochemical compounds in the extracts were screened as per standard methods. The tofu was preserved by immersion method for eight days under room temperature with an evaluation time point of two days. The microbial growth inhibitory activity was evaluated by indirect optical density (OD)-based enumeration, and the OD reduction by the extracts was calculated accordingly. The preservation potential was calculated from the changes of the physical characters on tofu evaluated by sensorial analysis. Galangal water extracts contained tannins and terpenoids. It concentration- dependently inhibited microbial growth on tofu, and the extract showed the best OD reduction at a concentration of 20%. It changed the color of tofu but maintained its texture. It also masked the odor of fresh tofu with the aromatic galangal scent. All three tested concentrations showed an equal preservation potential of 2 days. Our data suggested galangal water extract in the optimum concentration of 20% inhibited the microbial growth on tofu and potentially preserved it for two days. This moderate preservation effect might be attributable to its tannins and terpenoids content.

Documentation of Double and Triple Phytotherapeutic Remedies Against Diabetes Mellitus in Faisalabad City, Pakistan

Diabetic mellitus is a major health concern all over the world associated with many functional and structural ailments and is a serious metabolic disorder. Various natural products, plants and herbs have been used effectively for antidiabetic effects. The present study was conducted to know the ethnomedicinal practices (double and triple phytotherapy) for diabetes mellitus by having interviews with qualified herbalists (Hakeems) practising in Faisalabad city, Pakistan. It was revealed that a total of 40 species belonging to 25 families are presently used for the treatment of diabetic mellitus. Among these 4 families (Paparaceae, Convolvulaceae, Asteraceae, and Solanaceae) had two species each, and one family (Moraceae) had three species (Ficus religiosa, Ficus benghalansis, and Ficus carica) while another one family (Zingiberaceae) had four species (Zingiber officinale, Curcuma longa, Elettaria cardamomum, and Alpinia galangal) had been successfully used for the treatment of diabetic mellitus. In double therapy, ten out of 15 prescriptions were being used in pulverized form while the others were in liquid form for treatment. In triple therapy, 17 remedies out of 20 were used in powder form while the remaining others were used in mixing with liquid. According to the herbalists both double phytotherapy and triple phytotherapy were successfully used to reduce the sugar level and exerted antidiabetic effects. It was concluded that these plants may have potential ingredients which can be exploited in further studies for the development of an effective drug against diabetes.