The Effect of Alpinia Galanga Extract to Seminiferous Tubular Epithelial Thickness and Seminiferous Tubular Diameter of Male Mice Induced to Lead Acetate

Abstract

The purpose of this research was to determine the effect of Alpinia galanga extract for seminiferous tubular epithelial thickness, and seminiferous tubular diameter of mice induced to lead acetate. This study was experimental, 25 male mice (Mus musculus) strain Balb/C with average arround 30 gram were divided into 5 groups in a completely randomized design with different treatments for 24 days. The negative control group C (-) was given CMC-NA, the positive control group C(+) was given CMC-NA + lead acetate, groups P1, P2, and P3 were given galangal extract 200, 400, and 800 mg/kgBB + CMC-NA + lead acetate. Mice were sacrificed at a predetermined time and then histopathological preparations of testicular organs were made for epithelial thickness and seminiferous tubule diameter. Data analysis used ANOVA test and continued with LSD test. The result of the research galangal extract administration showed an increase in epithelial thickness and diameter significantly by the three treatment groups against the control (+) (p<0.05). group P2 which given galangal extract 200 mg/kgBw/day is the most effective to prevent epithelial thickness (59.83 ± 0.48; p<0.05) and diameter of seminiferous tubules (180.89 ± 0.85; p<0.05) because the result not significantly different from the control group (-) where epithelial thickness (59.64 ± 1.83; p<0.05) and seminiferous tubule diameter (180.71 ± 1.37; p<0.05) were given CMC- NA 0.2 ml/day. It can be concluded that giving galangal extract to male mice exposed to lead acetate was proven to maintain thick epithelium and seminiferous tubule diameter.