Abstract
Objectives: To determine the most effective solvent for extraction of antioxidant and potential compounds in Alpinia galanga, expressed via the percentage of components in the sample. Methods: A comparison test of 4 extractants, including water, hexane, chloroform and ethyl acetate, was conducted for the extraction procedure. In addition, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to perform antioxidant experiments. HPLC and GC-MS were then employed to determine quercetin content and scan the chemical compositions of the extract, respectively. Results: Ethyl acetate extract showed the strongest antioxidant activity (DPPH IC50=127.67ppm, ABTS IC50=54.82ppm), containing the highest level of quercetin (39.61mg/g DW of extract) and 2 major components of methyl oleate (15.36%) and methyl palmitate (12.01%). In the correlation analysis, the antioxidant activity of Alpinia galanga extracts was proportional to its total phenolic, flavonoid, and quercetin contents. Conclusion: Ethyl acetate produces the best performance in extracting anti-oxidative agents and quercetin in Alpinia galanga. The presence of quercetin and FAMEs in the extract highlighted the potentials of antioxidant, antihypertensive, anti-inflammatory, antibacterial, antiviral, and antidiabetic properties of Alpinia galanga.
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