A feeding trial was conducted to assess the effects of dietary Nannochloropsis sp. as powder or lipid on survival, growth performance and fatty acids content in Kuruma shrimp juvenile. Seven isonitrogenous, isolipidic and isocaloric diets were formulated to contain in first group graded levels of Nannochloropsis powder: 0% (CON, control diet), 4% (A), 7% (B) and 10% (C) dry matter then in second group algae oil proportionally extracted (AE, BE and CE, respectively). The feeding trial was conducted for 50 days. The results showed that the substitution of fish oil (FO) with algae powder diets A, B and C significantly (P < .05) improved shrimp growth compared to control, with the highest final body weight (FBW), weight gain (WG) and specific growth rate (SGR) recorded for C group. Shrimp fed diet containing algae lipid as FO replacement (1:1 dry matter) performed higher than control with an exception of group AE. The fatty acids analyse of shrimp tissue revealed that the group fed algae lipid had an improved profile of the fatty acids as the essential fatty acids was digested better than algae powder diet or CON. Fish oil partially replaced with dietary algae significantly increased the amount of n-3 highly unsaturated fatty acids (HUFA) in shrimp muscle. A small addition of DHA content was also observed indicating the occurrence of n-3 PUFA bioconversion from Nannochloropsis sp. into n-3 HUFA. Nannochloropsis powder can be used to substitute fish oil at the optimum level of 1.58 g algae lipid (26.33% FO) without negative effect on growth, survival and fatty acids profile of juvenile kuruma shrimp.